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The map label for this gene is purH [H]

Identifier: 222525471

GI number: 222525471

Start: 2812272

End: 2813789

Strand: Reverse

Name: purH [H]

Synonym: Chy400_2219

Alternate gene names: 222525471

Gene position: 2813789-2812272 (Counterclockwise)

Preceding gene: 222525472

Following gene: 222525470

Centisome position: 53.4

GC content: 57.64

Gene sequence:

>1518_bases
GTGCGCGCATTGATGAGCGTCTACGATAAAACGGGGATTGTCGAATTTGCTCAGGCTCTCCACAATCTTGGCATTGAGAT
TATCTCAACCGGTCAGACCCAACGTGTATTACGTGAAGCAGGTATCCCGGCTCTACCTGTCAGTGACGTCACCGGTTTTC
CCGAAATTCTTGATGGCCGGGTGAAAACGCTCCATCCTGCAATTCACGCCGGCTTGCTGGCGCGCCGTGATGTGCCGGCT
CATATGGCCGAACTGGCTGCCCATAATCTACAACCAATTGATCTAGTCGTAGTAAACCTCTACCCCTTCGCCGCTACCAT
TGCCCGCCCCGACGTGACAATGGCTGAAGCGCAAGAGCAGATTGACATCGGCGGTGTTGCATTGCTACGGGCGGCGGCCA
AGAATTTCCCGGCAGTGCTGGTACTGGTCGATCCGGCCGACTATGCCGGCGTGCTCGATGGCTTACGCGCTGGCGATGTG
CCATTGAGCGAACGACAGCGCCTGGCCGCAAAGGCGTTTGCCCATACCGCTGAGTACGATGCGACCATTGCCGCATATCT
CCGCACCGAGCCACTGCCCGATGTGCTGCCGTTGGCCTGGCGAAAATATCAGCCTCTGCGCTACGGCGAAAATCCTCACC
AGGCCGCTGCCCTGTACGGTGATTTTGGTGCCTTCTTCCATCAGTTGCACGGCAAAGAGCTAAGTTATAACAATATTCTT
GATACTGCCGCCGCGCAGGAGTTGATTGAAGAGTTTCCTGCGACTGAAGCGGCTGCGGTTGCCATCATTAAACACACCAA
TCCGTGTGGTGTAGCTATCGCTGCCGATCTCCATAGGGCCTGGGAAGCAGCATTTGCCACCGACCGCGAAGCTCCGTTCG
GTGGCATTATTGCGGTCAACCGACCAGTCGATATCGCGTTTGCCGAAGCGGTAGATGAAATCTTCTCGGAAATCATTATC
GCACCTGATTTTGCCCCCGATGCCCTGGCCTTGCTCCGACGCAAGAAGAATCGGCGGCTGCTCCAAAGCGTGCGCCCGAT
TACCGGTGCTGATCGCTGGCAACTGCGGAGCGTTCCCGGCGGTGTGTTGGTGCAAGAACCTGATCATGCTCCTCTGGTTG
CTGAAGAGTGGCGTGTCGTCACCAAACGTGCCCCAACCGATGCCGAAGCTGCTGCGCTCCGCTTCGCCTGGCGCGTAGTA
AAACACGTGAAGTCAAATGCGATTGTGTATGCAGCTCATGACCGCACTCTTGGCATCGGTGCCGGACAGATGAGCCGGGT
AGATAGCTCGCGCCTGGCTGTCTGGAAAGCCCAACAGGCCGGGATCGATCTGCGAGGCAGTGTCGTGGCAAGCGATGCGC
TCTTTCCCTTTGCTGATGGTGTTGAAGCGGCAATTGCTGCCGGTGCCACCGCCATTATTCAGCCCGGTGGCTCGGTACGC
GACGAAGAGGTGATCGCTGCGGCTGATGCCGCTGGCGCGGCGATGGTCTTTACCGGACGCCGCCATTTCCGCCATTAG

Upstream 100 bases:

>100_bases
GCTCGTTTGATCTGTGGTACAATGGGGCTACGCGACCCTGCCCGTCGCTGCGGGCAGGGTTTCGCTTTGGGTTGTCTCTG
TGATTGGTGAGGAGCGTGTT

Downstream 100 bases:

>100_bases
ATACACTGACAACCGTTACATAGCTGCCTTACAGAATATAAGGAGGTACACCGGACGCATGGTGTCCATAAGTGATACAG
AACTACTACCAGCTATCGTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MRALMSVYDKTGIVEFAQALHNLGIEIISTGQTQRVLREAGIPALPVSDVTGFPEILDGRVKTLHPAIHAGLLARRDVPA
HMAELAAHNLQPIDLVVVNLYPFAATIARPDVTMAEAQEQIDIGGVALLRAAAKNFPAVLVLVDPADYAGVLDGLRAGDV
PLSERQRLAAKAFAHTAEYDATIAAYLRTEPLPDVLPLAWRKYQPLRYGENPHQAAALYGDFGAFFHQLHGKELSYNNIL
DTAAAQELIEEFPATEAAAVAIIKHTNPCGVAIAADLHRAWEAAFATDREAPFGGIIAVNRPVDIAFAEAVDEIFSEIII
APDFAPDALALLRRKKNRRLLQSVRPITGADRWQLRSVPGGVLVQEPDHAPLVAEEWRVVTKRAPTDAEAAALRFAWRVV
KHVKSNAIVYAAHDRTLGIGAGQMSRVDSSRLAVWKAQQAGIDLRGSVVASDALFPFADGVEAAIAAGATAIIQPGGSVR
DEEVIAAADAAGAAMVFTGRRHFRH

Sequences:

>Translated_505_residues
MRALMSVYDKTGIVEFAQALHNLGIEIISTGQTQRVLREAGIPALPVSDVTGFPEILDGRVKTLHPAIHAGLLARRDVPA
HMAELAAHNLQPIDLVVVNLYPFAATIARPDVTMAEAQEQIDIGGVALLRAAAKNFPAVLVLVDPADYAGVLDGLRAGDV
PLSERQRLAAKAFAHTAEYDATIAAYLRTEPLPDVLPLAWRKYQPLRYGENPHQAAALYGDFGAFFHQLHGKELSYNNIL
DTAAAQELIEEFPATEAAAVAIIKHTNPCGVAIAADLHRAWEAAFATDREAPFGGIIAVNRPVDIAFAEAVDEIFSEIII
APDFAPDALALLRRKKNRRLLQSVRPITGADRWQLRSVPGGVLVQEPDHAPLVAEEWRVVTKRAPTDAEAAALRFAWRVV
KHVKSNAIVYAAHDRTLGIGAGQMSRVDSSRLAVWKAQQAGIDLRGSVVASDALFPFADGVEAAIAAGATAIIQPGGSVR
DEEVIAAADAAGAAMVFTGRRHFRH
>Mature_505_residues
MRALMSVYDKTGIVEFAQALHNLGIEIISTGQTQRVLREAGIPALPVSDVTGFPEILDGRVKTLHPAIHAGLLARRDVPA
HMAELAAHNLQPIDLVVVNLYPFAATIARPDVTMAEAQEQIDIGGVALLRAAAKNFPAVLVLVDPADYAGVLDGLRAGDV
PLSERQRLAAKAFAHTAEYDATIAAYLRTEPLPDVLPLAWRKYQPLRYGENPHQAAALYGDFGAFFHQLHGKELSYNNIL
DTAAAQELIEEFPATEAAAVAIIKHTNPCGVAIAADLHRAWEAAFATDREAPFGGIIAVNRPVDIAFAEAVDEIFSEIII
APDFAPDALALLRRKKNRRLLQSVRPITGADRWQLRSVPGGVLVQEPDHAPLVAEEWRVVTKRAPTDAEAAALRFAWRVV
KHVKSNAIVYAAHDRTLGIGAGQMSRVDSSRLAVWKAQQAGIDLRGSVVASDALFPFADGVEAAIAAGATAIIQPGGSVR
DEEVIAAADAAGAAMVFTGRRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=517, Percent_Identity=37.330754352031, Blast_Score=272, Evalue=6e-73,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=49.5274102079395, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71985564, Length=503, Percent_Identity=34.9900596421471, Blast_Score=262, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI71985574, Length=335, Percent_Identity=28.3582089552239, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323056, Length=458, Percent_Identity=37.9912663755458, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6323768, Length=461, Percent_Identity=36.6594360086768, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649832, Length=591, Percent_Identity=33.8409475465313, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54268; Mature: 54268

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALMSVYDKTGIVEFAQALHNLGIEIISTGQTQRVLREAGIPALPVSDVTGFPEILDGR
CCHHHHHHHHCCHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
VKTLHPAIHAGLLARRDVPAHMAELAAHNLQPIDLVVVNLYPFAATIARPDVTMAEAQEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHHHCCCCCHHHHHHHH
IDIGGVALLRAAAKNFPAVLVLVDPADYAGVLDGLRAGDVPLSERQRLAAKAFAHTAEYD
CCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
ATIAAYLRTEPLPDVLPLAWRKYQPLRYGENPHQAAALYGDFGAFFHQLHGKELSYNNIL
HHHEEEHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DTAAAQELIEEFPATEAAAVAIIKHTNPCGVAIAADLHRAWEAAFATDREAPFGGIIAVN
HHHHHHHHHHHCCCCCHHEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEEC
RPVDIAFAEAVDEIFSEIIIAPDFAPDALALLRRKKNRRLLQSVRPITGADRWQLRSVPG
CCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
GVLVQEPDHAPLVAEEWRVVTKRAPTDAEAAALRFAWRVVKHVKSNAIVYAAHDRTLGIG
CEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
AGQMSRVDSSRLAVWKAQQAGIDLRGSVVASDALFPFADGVEAAIAAGATAIIQPGGSVR
CCHHHHCCCCHHEEEEHHHCCCCCCCCEEECCCCCCCCCCHHHHHHCCCEEEECCCCCCC
DEEVIAAADAAGAAMVFTGRRHFRH
CCHHEEECCCCCEEEEEECHHHCCC
>Mature Secondary Structure
MRALMSVYDKTGIVEFAQALHNLGIEIISTGQTQRVLREAGIPALPVSDVTGFPEILDGR
CCHHHHHHHHCCHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
VKTLHPAIHAGLLARRDVPAHMAELAAHNLQPIDLVVVNLYPFAATIARPDVTMAEAQEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHHHCCCCCHHHHHHHH
IDIGGVALLRAAAKNFPAVLVLVDPADYAGVLDGLRAGDVPLSERQRLAAKAFAHTAEYD
CCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
ATIAAYLRTEPLPDVLPLAWRKYQPLRYGENPHQAAALYGDFGAFFHQLHGKELSYNNIL
HHHEEEHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DTAAAQELIEEFPATEAAAVAIIKHTNPCGVAIAADLHRAWEAAFATDREAPFGGIIAVN
HHHHHHHHHHHCCCCCHHEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEEC
RPVDIAFAEAVDEIFSEIIIAPDFAPDALALLRRKKNRRLLQSVRPITGADRWQLRSVPG
CCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
GVLVQEPDHAPLVAEEWRVVTKRAPTDAEAAALRFAWRVVKHVKSNAIVYAAHDRTLGIG
CEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
AGQMSRVDSSRLAVWKAQQAGIDLRGSVVASDALFPFADGVEAAIAAGATAIIQPGGSVR
CCHHHHCCCCHHEEEEHHHCCCCCCCCEEECCCCCCCCCCHHHHHHCCCEEEECCCCCCC
DEEVIAAADAAGAAMVFTGRRHFRH
CCHHEEECCCCCEEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA