Click here to switch to the map view.

The map label for this gene is atoS [H]

Identifier: 222525469

GI number: 222525469

Start: 2809570

End: 2811570

Strand: Reverse

Name: atoS [H]

Synonym: Chy400_2217

Alternate gene names: 222525469

Gene position: 2811570-2809570 (Counterclockwise)

Preceding gene: 222525470

Following gene: 222525464

Centisome position: 53.36

GC content: 55.97

Gene sequence:

>2001_bases
ATGAGCAGTTGGGCTGGCCACGTATTGGTTGTCGATGACGATCCGCAGTTACGCGACCTGTGTCGGCAAACGCTGGCACG
GGCCGGGTTTGTTGTAACAACGGCGGCTGATGTCACGACCGCGTTGGAGGCACTGGAACAAACGGTGTTTGATCTGGTTA
TGGTTGATCTAATCATGCCAGGGATGAATGGCCTCCAACTGCTCGAATTGATCCGATCACGTGACATCAGTGTGCCCATT
TTGATCGTCAGTGGTGCTGCTTCTATCGAGCAGATTTCCTACGCCATGCGCCTCGGTGCCCGTGGTCTGTTGATCAAACC
GTTCCGCGCCCAGGAATTGGCCGATATGGCCCGCGAGCTGGTTGCCAAACGGCAGGCCATCCGGGCCAGCGACCGCCTGG
CTGCGTTGCGCCCGCTGTTGCAAATCAGTCAGCATCTGTTGGCCGAGCTTGATCAGTCACGCCTGTACGCTTTGATTATC
GAAACGGTACGGGTTGAGGTACATGCTGAACGCGCTTCACTCATGCTATTGGAGCCGGATGAGCAGGCTCTCCGCATCGT
GGCCTGCACTGGCCTGCCTGACACAGTCGGCATTGGTGCCCTGATACCGGTAGACAGGAGTATCTCTGGATGGGTAGTTC
GTCATCGCCAGCCACTTCTGATCGATCAGCACCTTGTCTCATCACCCTGGTACAAAGAACTCAAGCCTCATCTCCTGTCC
CCTGAACTGACTACCGCGTTGTCGGTTCCTATCATGGCCGGCGAACGTGTGCTTGGTGTGCTTAACGCAGCAAAAGCGAC
GCTCGATGCGGCCTTTACGGCTGCTGATCAGGAACTATTACAACTACTCGCCGGGCAGGCAGCCGTGGCGATAGAGAATG
CCCGGTTGTACAGTGAGGTCTTGCGTTCAGAAGAGCGCTATCGCGCGCTCCTCCGTCATGCCAACGATGCAGTCTTACTG
CTCGATGCAACCGGGATGAACGTTCTCGATGCCAATTTAGCGTTGACCCGCCTGTCGGGGTACCCGGGTGAGGAAATACT
GCGGCTCAATCCTTGCCGATTGTTGCCAACCTTGCCTGCCCTGTTAAGCACTGCGGTCAACGGTCGCGCTGAGCACAGCG
AAATCGAAACGACACTCTGTACCGCCGATGGACATGAGACTGCGGTGGCAGTGAGCGTCAGTGTGGTGCCCGATGGCGAT
CAGCGCCTCTTTCTGGTCATTGCCCGCGATATTAGCGAGCGACAGCGTCTGGCGCGCGAACTTGCTCAGGCTGAAAAACT
TGCGGCGATTGGTCGTCTGGCTACCGCGATGGCGCACGAGATTAATAATCCACTGCAAGCGCTTTCAAGCACAGTCCACC
TCTTACAGCACTCTGATTTATCACCCGAACGACGCGATCTCTATTTGACCAAGGCTGCCGCTGAACTTGATGGGTTAATT
CAGCGCGTGCAACGCATTCTCGACATCTCACGCCCGAATCTCGATGGCCGTCGCCCGGTTGATCTCAATCAAGTCGTAAT
CGACGTGATTGCAACGTATCGCCAAACCCTGATCGCTCGCAACATTCGTTATATTGGTGAACTGACCTCAACGCTGCCGT
GGGTCACAGCCGTCATCAGCCATCTGAAACAGGTGTGTCAGACACTAGTCCAAAATGCAATCGAGGCCATGCCAGAGGGA
GGCACGCTCCGCATCCGCACCTATCTGCGTCCACCAGACGGTGAACCAGACCGGGCATCCTTCTGGCGTGCCGGCGGTGG
TTCGCGCCAGTTTCCTTCTGAGCAGGCGGTCGTGTTAGAGATTAGTGATACCGGGGTGGGCATTGCCCAGGAAGAGGTAC
CCAAGATTTTTGAACCGTTTTACTCGACGCGATTTGACGGGCTGGGAATAGGTTTGTCGCTCTGCTACAGTATCGTGGAT
TTTCACGGTGGCGAGTTATGGGTGCGTTCACAGCCTGAGCAGGGGACAACCTTTAGAGTTATTCTACCGATTGCCCAGTA
A

Upstream 100 bases:

>100_bases
GTGTTGCTGGAACAGCAAGGTATGACAGCAGAGGCGGTGTGGGCTGAACTGGCCCGTCGCTATAGCTCCTCGTAATCACT
GGAGATTGCGGGATAACCGT

Downstream 100 bases:

>100_bases
GGTTCAATCGAGTAAACCGGCAATTGGGCGGATCACCAGGCGCCGTTCACCGACATCAATTGCCACAATAAAATCACGGA
CCATCGGTACCAGCGCATCA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MSSWAGHVLVVDDDPQLRDLCRQTLARAGFVVTTAADVTTALEALEQTVFDLVMVDLIMPGMNGLQLLELIRSRDISVPI
LIVSGAASIEQISYAMRLGARGLLIKPFRAQELADMARELVAKRQAIRASDRLAALRPLLQISQHLLAELDQSRLYALII
ETVRVEVHAERASLMLLEPDEQALRIVACTGLPDTVGIGALIPVDRSISGWVVRHRQPLLIDQHLVSSPWYKELKPHLLS
PELTTALSVPIMAGERVLGVLNAAKATLDAAFTAADQELLQLLAGQAAVAIENARLYSEVLRSEERYRALLRHANDAVLL
LDATGMNVLDANLALTRLSGYPGEEILRLNPCRLLPTLPALLSTAVNGRAEHSEIETTLCTADGHETAVAVSVSVVPDGD
QRLFLVIARDISERQRLARELAQAEKLAAIGRLATAMAHEINNPLQALSSTVHLLQHSDLSPERRDLYLTKAAAELDGLI
QRVQRILDISRPNLDGRRPVDLNQVVIDVIATYRQTLIARNIRYIGELTSTLPWVTAVISHLKQVCQTLVQNAIEAMPEG
GTLRIRTYLRPPDGEPDRASFWRAGGGSRQFPSEQAVVLEISDTGVGIAQEEVPKIFEPFYSTRFDGLGIGLSLCYSIVD
FHGGELWVRSQPEQGTTFRVILPIAQ

Sequences:

>Translated_666_residues
MSSWAGHVLVVDDDPQLRDLCRQTLARAGFVVTTAADVTTALEALEQTVFDLVMVDLIMPGMNGLQLLELIRSRDISVPI
LIVSGAASIEQISYAMRLGARGLLIKPFRAQELADMARELVAKRQAIRASDRLAALRPLLQISQHLLAELDQSRLYALII
ETVRVEVHAERASLMLLEPDEQALRIVACTGLPDTVGIGALIPVDRSISGWVVRHRQPLLIDQHLVSSPWYKELKPHLLS
PELTTALSVPIMAGERVLGVLNAAKATLDAAFTAADQELLQLLAGQAAVAIENARLYSEVLRSEERYRALLRHANDAVLL
LDATGMNVLDANLALTRLSGYPGEEILRLNPCRLLPTLPALLSTAVNGRAEHSEIETTLCTADGHETAVAVSVSVVPDGD
QRLFLVIARDISERQRLARELAQAEKLAAIGRLATAMAHEINNPLQALSSTVHLLQHSDLSPERRDLYLTKAAAELDGLI
QRVQRILDISRPNLDGRRPVDLNQVVIDVIATYRQTLIARNIRYIGELTSTLPWVTAVISHLKQVCQTLVQNAIEAMPEG
GTLRIRTYLRPPDGEPDRASFWRAGGGSRQFPSEQAVVLEISDTGVGIAQEEVPKIFEPFYSTRFDGLGIGLSLCYSIVD
FHGGELWVRSQPEQGTTFRVILPIAQ
>Mature_665_residues
SSWAGHVLVVDDDPQLRDLCRQTLARAGFVVTTAADVTTALEALEQTVFDLVMVDLIMPGMNGLQLLELIRSRDISVPIL
IVSGAASIEQISYAMRLGARGLLIKPFRAQELADMARELVAKRQAIRASDRLAALRPLLQISQHLLAELDQSRLYALIIE
TVRVEVHAERASLMLLEPDEQALRIVACTGLPDTVGIGALIPVDRSISGWVVRHRQPLLIDQHLVSSPWYKELKPHLLSP
ELTTALSVPIMAGERVLGVLNAAKATLDAAFTAADQELLQLLAGQAAVAIENARLYSEVLRSEERYRALLRHANDAVLLL
DATGMNVLDANLALTRLSGYPGEEILRLNPCRLLPTLPALLSTAVNGRAEHSEIETTLCTADGHETAVAVSVSVVPDGDQ
RLFLVIARDISERQRLARELAQAEKLAAIGRLATAMAHEINNPLQALSSTVHLLQHSDLSPERRDLYLTKAAAELDGLIQ
RVQRILDISRPNLDGRRPVDLNQVVIDVIATYRQTLIARNIRYIGELTSTLPWVTAVISHLKQVCQTLVQNAIEAMPEGG
TLRIRTYLRPPDGEPDRASFWRAGGGSRQFPSEQAVVLEISDTGVGIAQEEVPKIFEPFYSTRFDGLGIGLSLCYSIVDF
HGGELWVRSQPEQGTTFRVILPIAQ

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=360, Percent_Identity=30.5555555555556, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790436, Length=261, Percent_Identity=29.1187739463602, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790300, Length=294, Percent_Identity=30.2721088435374, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787487, Length=103, Percent_Identity=37.8640776699029, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788713, Length=286, Percent_Identity=25.5244755244755, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790299, Length=120, Percent_Identity=34.1666666666667, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788550, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI48994928, Length=350, Percent_Identity=23.1428571428571, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788191, Length=118, Percent_Identity=36.4406779661017, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786784, Length=121, Percent_Identity=28.9256198347107, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081816, Length=255, Percent_Identity=27.843137254902, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786600, Length=288, Percent_Identity=24.3055555555556, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1790437, Length=148, Percent_Identity=30.4054054054054, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=107, Percent_Identity=31.7757009345794, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73075; Mature: 72944

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSWAGHVLVVDDDPQLRDLCRQTLARAGFVVTTAADVTTALEALEQTVFDLVMVDLIMP
CCCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
GMNGLQLLELIRSRDISVPILIVSGAASIEQISYAMRLGARGLLIKPFRAQELADMAREL
CCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHH
VAKRQAIRASDRLAALRPLLQISQHLLAELDQSRLYALIIETVRVEVHAERASLMLLEPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCC
EQALRIVACTGLPDTVGIGALIPVDRSISGWVVRHRQPLLIDQHLVSSPWYKELKPHLLS
HHCEEEEEECCCCCCCCCCEEEECCCCCCCCEEECCCCCEEHHHHHCCCCHHHCCCCCCC
PELTTALSVPIMAGERVLGVLNAAKATLDAAFTAADQELLQLLAGQAAVAIENARLYSEV
CCHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHH
LRSEERYRALLRHANDAVLLLDATGMNVLDANLALTRLSGYPGEEILRLNPCRLLPTLPA
HHCHHHHHHHHHCCCCEEEEEECCCCCEEECCHHEEECCCCCCCCEEEECCHHHHHHHHH
LLSTAVNGRAEHSEIETTLCTADGHETAVAVSVSVVPDGDQRLFLVIARDISERQRLARE
HHHHHHCCCCCHHHHHEEEEECCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHH
LAQAEKLAAIGRLATAMAHEINNPLQALSSTVHLLQHSDLSPERRDLYLTKAAAELDGLI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHH
QRVQRILDISRPNLDGRRPVDLNQVVIDVIATYRQTLIARNIRYIGELTSTLPWVTAVIS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLKQVCQTLVQNAIEAMPEGGTLRIRTYLRPPDGEPDRASFWRAGGGSRQFPSEQAVVLE
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCEEEEE
ISDTGVGIAQEEVPKIFEPFYSTRFDGLGIGLSLCYSIVDFHGGELWVRSQPEQGTTFRV
EECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
ILPIAQ
EEECCC
>Mature Secondary Structure 
SSWAGHVLVVDDDPQLRDLCRQTLARAGFVVTTAADVTTALEALEQTVFDLVMVDLIMP
CCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
GMNGLQLLELIRSRDISVPILIVSGAASIEQISYAMRLGARGLLIKPFRAQELADMAREL
CCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHH
VAKRQAIRASDRLAALRPLLQISQHLLAELDQSRLYALIIETVRVEVHAERASLMLLEPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCC
EQALRIVACTGLPDTVGIGALIPVDRSISGWVVRHRQPLLIDQHLVSSPWYKELKPHLLS
HHCEEEEEECCCCCCCCCCEEEECCCCCCCCEEECCCCCEEHHHHHCCCCHHHCCCCCCC
PELTTALSVPIMAGERVLGVLNAAKATLDAAFTAADQELLQLLAGQAAVAIENARLYSEV
CCHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHH
LRSEERYRALLRHANDAVLLLDATGMNVLDANLALTRLSGYPGEEILRLNPCRLLPTLPA
HHCHHHHHHHHHCCCCEEEEEECCCCCEEECCHHEEECCCCCCCCEEEECCHHHHHHHHH
LLSTAVNGRAEHSEIETTLCTADGHETAVAVSVSVVPDGDQRLFLVIARDISERQRLARE
HHHHHHCCCCCHHHHHEEEEECCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHH
LAQAEKLAAIGRLATAMAHEINNPLQALSSTVHLLQHSDLSPERRDLYLTKAAAELDGLI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHH
QRVQRILDISRPNLDGRRPVDLNQVVIDVIATYRQTLIARNIRYIGELTSTLPWVTAVIS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLKQVCQTLVQNAIEAMPEGGTLRIRTYLRPPDGEPDRASFWRAGGGSRQFPSEQAVVLE
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCEEEEE
ISDTGVGIAQEEVPKIFEPFYSTRFDGLGIGLSLCYSIVDFHGGELWVRSQPEQGTTFRV
EECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
ILPIAQ
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]