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The map label for this gene is cca [H]

Identifier: 222525428

GI number: 222525428

Start: 2754143

End: 2755405

Strand: Reverse

Name: cca [H]

Synonym: Chy400_2173

Alternate gene names: 222525428

Gene position: 2755405-2754143 (Counterclockwise)

Preceding gene: 222525430

Following gene: 222525427

Centisome position: 52.3

GC content: 58.27

Gene sequence:

>1263_bases
ATGAAAGATCTGATAACGCGACTCGATCCCAAATTACAACATCTGCTGGCTCGTGCTGCCGCCCTGGCCGCAGATGTTGG
CGCGACATTGTGGCTGGTTGGCGGTGTTGTACGCGATCTCTGGCTTGAGCTGCCGATTGGCCGTGACATTGATCTGGCTG
TTGAGGGAGACATCAATCAACTTATTGTTCCTTTAGCGGATGCCTGGGACGGTACCATCACGGCGCGCCACCCGGCGTTT
GGGACGGCGACGATCACTGTCGGGCACTGGGTGATCGATCTGGCACAGACGCGCACCGAACGTTACCCCAAACCCGCAAT
ACTACCCGAAGTGCAGCCGGCACCCCTAACGAAAGACCTTTACCGACGAGATTTCAGTATCAACGCCATGGCCGTCCAGA
TTATTGCCGATGGCGATCAGCTCTTATCGGCGCCGCTCATTGACCCCCTGGGTGGAGTTGCCGATCTGACAGCCCGCCGC
CTGCGTCTCTTGCACGATCAGAGCCTGCGCGATGATCCAACTCGCCTCTTGCGCGGGGTACGATTAGCCGCACGCCTGCA
ACTGACGCCCGATGCTGCAACAGCCGCTCAGATCGCCGACGCACTGGAAGCAGGTTACCTGGCCTTGCTCTCACCTGAAC
GGATACAGACTGAACTCTGTCTGACACTGGAGGAACCCGATCCGGCTGCTGTGATTGCACTGGCCGATCAATGGGGACTC
ACCCCTCACGTCATCCCCGGCCTGCACTGGACGCCGGCTCTGGCCGAGCGGCTAAGCCGCTATCGCCAGGACACCAGCGA
GATATCCAGACTGGCACCGGACGGATTGGTAATCGCCGGTCTCGTGCTCTACGACCTGAGCGATGATGCACTGACACAGG
TTCTACATCGCTACCATCTTCCTGCGCCGGCAAGGAACCTGATCCAGGACCTGATCGCACTACGCCCGCACATCGCTACC
ATTGCCGCCGCTACCAGTCCGAGTGCTATCGATACGTTACTACAACCCTATAGCATAGCTGCGATTGCTGTGCTACACTA
CGCGACAGAAACTTCAATTCAGCAAGTGGTATCACGCTATTTACACGAATGGCGACCATTGCGCCCCCCACTCAACGGCG
ATGATCTGCGCCATTTAGGGGTACCAACCGGCCCGCAAATTGGGCAACTTCTGCGCCGGCTGCGCGCAGCTACACTCGAT
GGCCTGATAACAGATCGTACAACTGCCGAACAATGGGTTCGCCGGCATCTGGTCGATTCCTGA

Upstream 100 bases:

>100_bases
ACCACTGACCTCCACGATGTCAACGTGGCGCTCTAACCAACTGAGCTAATCGCCCGACGGTCAATATCGTATCATTGAAT
GCCTGCTCTGTCAAGCGCAC

Downstream 100 bases:

>100_bases
TAGGTGAGAGAGGAAAAATCTGTGGATGCAATGCTGCAACTCTTGAAAGATCTGACCGAGGCTCCCGGCGCTTCCGGCTA
TGAGAGTCCGGTTCGCGCAG

Product: polynucleotide adenylyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKDLITRLDPKLQHLLARAAALAADVGATLWLVGGVVRDLWLELPIGRDIDLAVEGDINQLIVPLADAWDGTITARHPAF
GTATITVGHWVIDLAQTRTERYPKPAILPEVQPAPLTKDLYRRDFSINAMAVQIIADGDQLLSAPLIDPLGGVADLTARR
LRLLHDQSLRDDPTRLLRGVRLAARLQLTPDAATAAQIADALEAGYLALLSPERIQTELCLTLEEPDPAAVIALADQWGL
TPHVIPGLHWTPALAERLSRYRQDTSEISRLAPDGLVIAGLVLYDLSDDALTQVLHRYHLPAPARNLIQDLIALRPHIAT
IAAATSPSAIDTLLQPYSIAAIAVLHYATETSIQQVVSRYLHEWRPLRPPLNGDDLRHLGVPTGPQIGQLLRRLRAATLD
GLITDRTTAEQWVRRHLVDS

Sequences:

>Translated_420_residues
MKDLITRLDPKLQHLLARAAALAADVGATLWLVGGVVRDLWLELPIGRDIDLAVEGDINQLIVPLADAWDGTITARHPAF
GTATITVGHWVIDLAQTRTERYPKPAILPEVQPAPLTKDLYRRDFSINAMAVQIIADGDQLLSAPLIDPLGGVADLTARR
LRLLHDQSLRDDPTRLLRGVRLAARLQLTPDAATAAQIADALEAGYLALLSPERIQTELCLTLEEPDPAAVIALADQWGL
TPHVIPGLHWTPALAERLSRYRQDTSEISRLAPDGLVIAGLVLYDLSDDALTQVLHRYHLPAPARNLIQDLIALRPHIAT
IAAATSPSAIDTLLQPYSIAAIAVLHYATETSIQQVVSRYLHEWRPLRPPLNGDDLRHLGVPTGPQIGQLLRRLRAATLD
GLITDRTTAEQWVRRHLVDS
>Mature_420_residues
MKDLITRLDPKLQHLLARAAALAADVGATLWLVGGVVRDLWLELPIGRDIDLAVEGDINQLIVPLADAWDGTITARHPAF
GTATITVGHWVIDLAQTRTERYPKPAILPEVQPAPLTKDLYRRDFSINAMAVQIIADGDQLLSAPLIDPLGGVADLTARR
LRLLHDQSLRDDPTRLLRGVRLAARLQLTPDAATAAQIADALEAGYLALLSPERIQTELCLTLEEPDPAAVIALADQWGL
TPHVIPGLHWTPALAERLSRYRQDTSEISRLAPDGLVIAGLVLYDLSDDALTQVLHRYHLPAPARNLIQDLIALRPHIAT
IAAATSPSAIDTLLQPYSIAAIAVLHYATETSIQQVVSRYLHEWRPLRPPLNGDDLRHLGVPTGPQIGQLLRRLRAATLD
GLITDRTTAEQWVRRHLVDS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=206, Percent_Identity=33.9805825242718, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46025; Mature: 46025

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLITRLDPKLQHLLARAAALAADVGATLWLVGGVVRDLWLELPIGRDIDLAVEGDINQ
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
LIVPLADAWDGTITARHPAFGTATITVGHWVIDLAQTRTERYPKPAILPEVQPAPLTKDL
HHEEHHCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
YRRDFSINAMAVQIIADGDQLLSAPLIDPLGGVADLTARRLRLLHDQSLRDDPTRLLRGV
HHCCCCCCEEEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RLAARLQLTPDAATAAQIADALEAGYLALLSPERIQTELCLTLEEPDPAAVIALADQWGL
HHHHHEEECCCHHHHHHHHHHHHHCEEEEECHHHHCEEEEEEECCCCCCEEEEEEHHCCC
TPHVIPGLHWTPALAERLSRYRQDTSEISRLAPDGLVIAGLVLYDLSDDALTQVLHRYHL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
PAPARNLIQDLIALRPHIATIAAATSPSAIDTLLQPYSIAAIAVLHYATETSIQQVVSRY
CCCHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LHEWRPLRPPLNGDDLRHLGVPTGPQIGQLLRRLRAATLDGLITDRTTAEQWVRRHLVDS
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDLITRLDPKLQHLLARAAALAADVGATLWLVGGVVRDLWLELPIGRDIDLAVEGDINQ
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
LIVPLADAWDGTITARHPAFGTATITVGHWVIDLAQTRTERYPKPAILPEVQPAPLTKDL
HHEEHHCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
YRRDFSINAMAVQIIADGDQLLSAPLIDPLGGVADLTARRLRLLHDQSLRDDPTRLLRGV
HHCCCCCCEEEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RLAARLQLTPDAATAAQIADALEAGYLALLSPERIQTELCLTLEEPDPAAVIALADQWGL
HHHHHEEECCCHHHHHHHHHHHHHCEEEEECHHHHCEEEEEEECCCCCCEEEEEEHHCCC
TPHVIPGLHWTPALAERLSRYRQDTSEISRLAPDGLVIAGLVLYDLSDDALTQVLHRYHL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
PAPARNLIQDLIALRPHIATIAAATSPSAIDTLLQPYSIAAIAVLHYATETSIQQVVSRY
CCCHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LHEWRPLRPPLNGDDLRHLGVPTGPQIGQLLRRLRAATLDGLITDRTTAEQWVRRHLVDS
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA