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The map label for this gene is rsmE [H]

Identifier: 222525248

GI number: 222525248

Start: 2523250

End: 2524008

Strand: Reverse

Name: rsmE [H]

Synonym: Chy400_1988

Alternate gene names: 222525248

Gene position: 2524008-2523250 (Counterclockwise)

Preceding gene: 222525249

Following gene: 222525247

Centisome position: 47.9

GC content: 59.03

Gene sequence:

>759_bases
ATGGTTGATGACACACTCGCAATTCCCAACACACGCCGCTTCTTCGTCAATCCTGGCTGGCTACGCACCGATCAGGTAGA
GATTGATGATGCGGCCATCATTCATCAATGGGAGCGTGTCTTACGCCTCCGCGCCGGTGATCGGGTGATGCTGCTCGACG
GACAGGGATTGGCGGCAATCGTTGAGATCACTCACATTGATCGGCGACGCGCTCGCGGTCAGGTAGTGGCTCGCTGCCAG
GCTTCCGGCGAGCCGGCTGTGACCTTTGATCTCTATCTGGCCCTCATCCGTCCTGAGCGCTTCGAGTGGGCAATCCAGAA
AGCTGTTGAGCTAGGGGTATACCGGATTGTACCGGTGACCTTCGCCCGTTCGCTCATCACCGGTCGGATTGACGAACATC
GTCTGGCGCGCTGGCAACGCATTGCTACCGAAGCAGCCGAGCAGGCCGGACGCGGACGATTGCCGGTTGTCGCTGAACCG
CAGTCGTTCGCGGCGGCACTGACCAGAGCGCATACCGCCGACCTGGCTATTCTGCTCGATGAACAGGCAACGGTGCATCT
ACGCACAGTGCTGCCGCCACAACCATCGTTACGCCAGATTGCCATTTATTCGGGACCCGAAGGTGGCATTGATCCCGCCG
AACGAACGGCGGCGCATCAGCACGGCATTCAATCGGTGTCACTGGGACCACGCATTTTACGGGCCGAAACGGCACCGCTG
GTAGCACTGGCCATGCTTCAATATGCACTTCAATACTAG

Upstream 100 bases:

>100_bases
ATGATGCAACCCTGTTGCGTTCATATATTCCCGATATACCGGCGTTAATCAGCACACTTGGCGTATCACCGGGTACCGGT
CCAATCTTCCCCTAAAGCCG

Downstream 100 bases:

>100_bases
AAAAGATACTGCTATGGAACGGGTGTGGGCAATTATCGGCAAAATCGTGCAATGGCTGGCTGTCGTGACCGTGGCGTTTC
TCGGCGGGTGGATTACCGGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MVDDTLAIPNTRRFFVNPGWLRTDQVEIDDAAIIHQWERVLRLRAGDRVMLLDGQGLAAIVEITHIDRRRARGQVVARCQ
ASGEPAVTFDLYLALIRPERFEWAIQKAVELGVYRIVPVTFARSLITGRIDEHRLARWQRIATEAAEQAGRGRLPVVAEP
QSFAAALTRAHTADLAILLDEQATVHLRTVLPPQPSLRQIAIYSGPEGGIDPAERTAAHQHGIQSVSLGPRILRAETAPL
VALAMLQYALQY

Sequences:

>Translated_252_residues
MVDDTLAIPNTRRFFVNPGWLRTDQVEIDDAAIIHQWERVLRLRAGDRVMLLDGQGLAAIVEITHIDRRRARGQVVARCQ
ASGEPAVTFDLYLALIRPERFEWAIQKAVELGVYRIVPVTFARSLITGRIDEHRLARWQRIATEAAEQAGRGRLPVVAEP
QSFAAALTRAHTADLAILLDEQATVHLRTVLPPQPSLRQIAIYSGPEGGIDPAERTAAHQHGIQSVSLGPRILRAETAPL
VALAMLQYALQY
>Mature_252_residues
MVDDTLAIPNTRRFFVNPGWLRTDQVEIDDAAIIHQWERVLRLRAGDRVMLLDGQGLAAIVEITHIDRRRARGQVVARCQ
ASGEPAVTFDLYLALIRPERFEWAIQKAVELGVYRIVPVTFARSLITGRIDEHRLARWQRIATEAAEQAGRGRLPVVAEP
QSFAAALTRAHTADLAILLDEQATVHLRTVLPPQPSLRQIAIYSGPEGGIDPAERTAAHQHGIQSVSLGPRILRAETAPL
VALAMLQYALQY

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=224, Percent_Identity=31.25, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28020; Mature: 28020

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDDTLAIPNTRRFFVNPGWLRTDQVEIDDAAIIHQWERVLRLRAGDRVMLLDGQGLAAI
CCCCCCCCCCCCEEEECCCEEECCCEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
VEITHIDRRRARGQVVARCQASGEPAVTFDLYLALIRPERFEWAIQKAVELGVYRIVPVT
EEEHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCHHEEHHHH
FARSLITGRIDEHRLARWQRIATEAAEQAGRGRLPVVAEPQSFAAALTRAHTADLAILLD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCEEEEEE
EQATVHLRTVLPPQPSLRQIAIYSGPEGGIDPAERTAAHQHGIQSVSLGPRILRAETAPL
CCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEHHCCCHH
VALAMLQYALQY
HHHHHHHHHHCC
>Mature Secondary Structure
MVDDTLAIPNTRRFFVNPGWLRTDQVEIDDAAIIHQWERVLRLRAGDRVMLLDGQGLAAI
CCCCCCCCCCCCEEEECCCEEECCCEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
VEITHIDRRRARGQVVARCQASGEPAVTFDLYLALIRPERFEWAIQKAVELGVYRIVPVT
EEEHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHCHHEEHHHH
FARSLITGRIDEHRLARWQRIATEAAEQAGRGRLPVVAEPQSFAAALTRAHTADLAILLD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCEEEEEE
EQATVHLRTVLPPQPSLRQIAIYSGPEGGIDPAERTAAHQHGIQSVSLGPRILRAETAPL
CCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEHHCCCHH
VALAMLQYALQY
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]