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The map label for this gene is 222525221

Identifier: 222525221

GI number: 222525221

Start: 2489571

End: 2490887

Strand: Direct

Name: 222525221

Synonym: Chy400_1961

Alternate gene names: NA

Gene position: 2489571-2490887 (Clockwise)

Preceding gene: 222525220

Following gene: 222525222

Centisome position: 47.25

GC content: 53.3

Gene sequence:

>1317_bases
ATGGCGGATAAGTGGGGGACAGTGAGGCTTGGAGATGTGGCTACGATCAATCCAGATGCAATTGGTGCTAATTGGCCCTT
CTTACACATAAGATACATAGACATTTCGTCAGTAGGTGAAGGAACAATCATTGAGAAACCAAGTCAAATCAGCCTTTCAG
AAGCTCCAAGCCGCGCCAAGCGTCTAATTCGTGAGGGTGACACAGTTCTTTCAATGGTACGCCCCAACAGGCGCTCAATG
TTCTTCGTCACTACGTTTGAACCTGACTTGGTTGTGTCTACTGGATTTGCAGTCCTACGACCAAAACCCAAAGTTATTCA
TCCCCGTTATCTGTACGCTTGCGTGTTCGATAGAGCTTTCACTGACTATTTGGTTTCAAGGGAAAAGGGTGCTGCTTACC
CTGCAGTGCTATCGGAGGACATTGCGGATGCCAAGATCCCTTTCCCCCCCCTCCCCGAACAACGCGCCATTGCCCACATC
CTCGGCACGCTGGACGACAAAATCGAGCTGAACCGGCGCATGAGCGAGACGCTGGAACAGATGGCGCGGGCACTGTTCAA
GGCGTGGTTCGTCGACTTCGAGCCGGTGCGGGCGAAGATAGAGTGCCGCTGGCAGCGCGGTCAATCCCTGCCCGGTCTGC
CTGCCCATTTCTACGACCTGTTCCCCGAACGCCTGGTAGACTCGGAGTTGGGGGAGATTCCGGAGGGATGGGGGGTGGGG
AGGCTGTCAGAACTAATCGAATTGAACCCACCGCGAGTACTAAGGAAGGGTGAAGTTGCTCCTTACCTTGATATGGCAAA
TATGCCAACTCGTGGTCACGTCCCTGGCGATGTTGTTGATCGTCCATTTGGATCAGGCACCCGTTTCATAAACGGTGATA
CGCTCCTTGCCCGAATCACTCCATGTTTGGAGAATGGGAAGACTGCATTCGTTGATTTTCTCCGAAATGGGCAAGTTGGT
TGGGGTTCAACAGAGTACATTGTACTAAGACCGAGAGAGCCTTTGCCTGCCGAGTTTGCTTACTGCTTGGCACGCAGTGA
GAATTTCCGTGACTTCGCCATTCAGAATATGACTGGCACAAGTGGTAGGCAGCGGGTACAAACAGAAGCTATTGCTCATT
ACCTGCTTGTCGCACCACCTGCTCCTGTGGCCGAGGCGTTCGGCAGAACCGTAAAGCAGCTATTTGCCAGGGCAACTAGA
GCATCGTGCGAATCCCGCACCCTCGCCGCGCTGCGCGATGCGCTGCTGCCGAAGCTCATCCGTGGTGAGATACGAGTGAA
GGATGCGGAAAAGTTTTTGCAGGAGAGGGGGTTATGA

Upstream 100 bases:

>100_bases
CGCGAAGCGACTGGCAACGCCGTAATAGGTGTTAGCCTGTGAACTGCACGTGAATATCTTGAGTGAAGAGAGTGAAGTGT
TGTGAAATGGGAGTTAAGCT

Downstream 100 bases:

>100_bases
AGTGGGAGGTAAAGCGTTTGCTGGAGAAGGCGCTTGATTCGATTCTGCTTGCTATTGAGATCTTCAATCGTCCCTATGAT
CGAGGAAGAGTCAGCGCAAC

Product: restriction modification system DNA specificity domain-containing protein

Products: NA

Alternate protein names: S.HindVIIP; Type I restriction enzyme HindVIIP specificity protein; S protein [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MADKWGTVRLGDVATINPDAIGANWPFLHIRYIDISSVGEGTIIEKPSQISLSEAPSRAKRLIREGDTVLSMVRPNRRSM
FFVTTFEPDLVVSTGFAVLRPKPKVIHPRYLYACVFDRAFTDYLVSREKGAAYPAVLSEDIADAKIPFPPLPEQRAIAHI
LGTLDDKIELNRRMSETLEQMARALFKAWFVDFEPVRAKIECRWQRGQSLPGLPAHFYDLFPERLVDSELGEIPEGWGVG
RLSELIELNPPRVLRKGEVAPYLDMANMPTRGHVPGDVVDRPFGSGTRFINGDTLLARITPCLENGKTAFVDFLRNGQVG
WGSTEYIVLRPREPLPAEFAYCLARSENFRDFAIQNMTGTSGRQRVQTEAIAHYLLVAPPAPVAEAFGRTVKQLFARATR
ASCESRTLAALRDALLPKLIRGEIRVKDAEKFLQERGL

Sequences:

>Translated_438_residues
MADKWGTVRLGDVATINPDAIGANWPFLHIRYIDISSVGEGTIIEKPSQISLSEAPSRAKRLIREGDTVLSMVRPNRRSM
FFVTTFEPDLVVSTGFAVLRPKPKVIHPRYLYACVFDRAFTDYLVSREKGAAYPAVLSEDIADAKIPFPPLPEQRAIAHI
LGTLDDKIELNRRMSETLEQMARALFKAWFVDFEPVRAKIECRWQRGQSLPGLPAHFYDLFPERLVDSELGEIPEGWGVG
RLSELIELNPPRVLRKGEVAPYLDMANMPTRGHVPGDVVDRPFGSGTRFINGDTLLARITPCLENGKTAFVDFLRNGQVG
WGSTEYIVLRPREPLPAEFAYCLARSENFRDFAIQNMTGTSGRQRVQTEAIAHYLLVAPPAPVAEAFGRTVKQLFARATR
ASCESRTLAALRDALLPKLIRGEIRVKDAEKFLQERGL
>Mature_437_residues
ADKWGTVRLGDVATINPDAIGANWPFLHIRYIDISSVGEGTIIEKPSQISLSEAPSRAKRLIREGDTVLSMVRPNRRSMF
FVTTFEPDLVVSTGFAVLRPKPKVIHPRYLYACVFDRAFTDYLVSREKGAAYPAVLSEDIADAKIPFPPLPEQRAIAHIL
GTLDDKIELNRRMSETLEQMARALFKAWFVDFEPVRAKIECRWQRGQSLPGLPAHFYDLFPERLVDSELGEIPEGWGVGR
LSELIELNPPRVLRKGEVAPYLDMANMPTRGHVPGDVVDRPFGSGTRFINGDTLLARITPCLENGKTAFVDFLRNGQVGW
GSTEYIVLRPREPLPAEFAYCLARSENFRDFAIQNMTGTSGRQRVQTEAIAHYLLVAPPAPVAEAFGRTVKQLFARATRA
SCESRTLAALRDALLPKLIRGEIRVKDAEKFLQERGL

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 49089; Mature: 48958

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKWGTVRLGDVATINPDAIGANWPFLHIRYIDISSVGEGTIIEKPSQISLSEAPSRAK
CCCCCCCEEECCEEEECCCCCCCCCCEEEEEEEEECCCCCCCEECCCCCCCHHCCHHHHH
RLIREGDTVLSMVRPNRRSMFFVTTFEPDLVVSTGFAVLRPKPKVIHPRYLYACVFDRAF
HHHHCCCHHEEHCCCCCCEEEEEEECCCCEEEECCEEEECCCCCEECCHHHHHHHHHHHH
TDYLVSREKGAAYPAVLSEDIADAKIPFPPLPEQRAIAHILGTLDDKIELNRRMSETLEQ
HHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MARALFKAWFVDFEPVRAKIECRWQRGQSLPGLPAHFYDLFPERLVDSELGEIPEGWGVG
HHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
RLSELIELNPPRVLRKGEVAPYLDMANMPTRGHVPGDVVDRPFGSGTRFINGDTLLARIT
HHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEECCCEEEEHHH
PCLENGKTAFVDFLRNGQVGWGSTEYIVLRPREPLPAEFAYCLARSENFRDFAIQNMTGT
HHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHEEECCCCC
SGRQRVQTEAIAHYLLVAPPAPVAEAFGRTVKQLFARATRASCESRTLAALRDALLPKLI
CHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGEIRVKDAEKFLQERGL
HCCEEEHHHHHHHHHCCC
>Mature Secondary Structure 
ADKWGTVRLGDVATINPDAIGANWPFLHIRYIDISSVGEGTIIEKPSQISLSEAPSRAK
CCCCCCEEECCEEEECCCCCCCCCCEEEEEEEEECCCCCCCEECCCCCCCHHCCHHHHH
RLIREGDTVLSMVRPNRRSMFFVTTFEPDLVVSTGFAVLRPKPKVIHPRYLYACVFDRAF
HHHHCCCHHEEHCCCCCCEEEEEEECCCCEEEECCEEEECCCCCEECCHHHHHHHHHHHH
TDYLVSREKGAAYPAVLSEDIADAKIPFPPLPEQRAIAHILGTLDDKIELNRRMSETLEQ
HHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MARALFKAWFVDFEPVRAKIECRWQRGQSLPGLPAHFYDLFPERLVDSELGEIPEGWGVG
HHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
RLSELIELNPPRVLRKGEVAPYLDMANMPTRGHVPGDVVDRPFGSGTRFINGDTLLARIT
HHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEECCCEEEEHHH
PCLENGKTAFVDFLRNGQVGWGSTEYIVLRPREPLPAEFAYCLARSENFRDFAIQNMTGT
HHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHEEECCCCC
SGRQRVQTEAIAHYLLVAPPAPVAEAFGRTVKQLFARATRASCESRTLAALRDALLPKLI
CHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGEIRVKDAEKFLQERGL
HCCEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]