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The map label for this gene is qacF [H]

Identifier: 222525188

GI number: 222525188

Start: 2445395

End: 2445727

Strand: Direct

Name: qacF [H]

Synonym: Chy400_1927

Alternate gene names: 222525188

Gene position: 2445395-2445727 (Clockwise)

Preceding gene: 222525187

Following gene: 222525189

Centisome position: 46.41

GC content: 52.85

Gene sequence:

>333_bases
ATGAAATACTGGCTCTACCTGATGCTGGCAATTCTGACCGAAGTTGTGGCTACCTCAGCGCTCAAAGCCAGCGCCGGCTT
TAGCCGTCCCTTGCCATCACTGATCGTTGTAGCGGGCTATGCAATCTCGTTTTACGCGATGTCGCTGGCGCTGGAGGCAA
TACCCGTTGGCATCGCTTATGCAGTCTGGTCGGGAATCGGGATTGTGCTGATCACGGTTGCGGCCTGGTTCCTGTACGGC
CAGCGGCTTGATGTATGGGCACTCATTGGGATTGGGTTCATTATTGTCGGGGTGGTTATTTTGAATCTTTTGTCAAAAGT
TGAGGTGCGCTAA

Upstream 100 bases:

>100_bases
GGGTTATGAAACTATATTGTGGATGCATCAACAGCACCGTAGCGGCATATTTGTCTATCCTGTCCTGTATTGAATGCCAT
GAACACTGTGGAGAACCATC

Downstream 100 bases:

>100_bases
TAAACTAATGTTTATTGATTTCGATCATCACAACTGCTAGAATGACAGTGTTCAATAGCAGATCAATCGTTCCGGCACTG
TGATGAAACTAACCGTTTTA

Product: small multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Quaternary ammonium determinant F [H]

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MKYWLYLMLAILTEVVATSALKASAGFSRPLPSLIVVAGYAISFYAMSLALEAIPVGIAYAVWSGIGIVLITVAAWFLYG
QRLDVWALIGIGFIIVGVVILNLLSKVEVR

Sequences:

>Translated_110_residues
MKYWLYLMLAILTEVVATSALKASAGFSRPLPSLIVVAGYAISFYAMSLALEAIPVGIAYAVWSGIGIVLITVAAWFLYG
QRLDVWALIGIGFIIVGVVILNLLSKVEVR
>Mature_110_residues
MKYWLYLMLAILTEVVATSALKASAGFSRPLPSLIVVAGYAISFYAMSLALEAIPVGIAYAVWSGIGIVLITVAAWFLYG
QRLDVWALIGIGFIIVGVVILNLLSKVEVR

Specific function: Multidrug exporter. Is implicated for the resistance to bacteriocidal quaternary ammonium compounds [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=106, Percent_Identity=49.0566037735849, Blast_Score=99, Evalue=6e-23,
Organism=Escherichia coli, GI1787883, Length=98, Percent_Identity=38.7755102040816, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI87082378, Length=100, Percent_Identity=38, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 11863; Mature: 11863

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYWLYLMLAILTEVVATSALKASAGFSRPLPSLIVVAGYAISFYAMSLALEAIPVGIAY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVWSGIGIVLITVAAWFLYGQRLDVWALIGIGFIIVGVVILNLLSKVEVR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYWLYLMLAILTEVVATSALKASAGFSRPLPSLIVVAGYAISFYAMSLALEAIPVGIAY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVWSGIGIVLITVAAWFLYGQRLDVWALIGIGFIIVGVVILNLLSKVEVR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9756755 [H]