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The map label for this gene is nuoL [H]

Identifier: 222525186

GI number: 222525186

Start: 2442178

End: 2443761

Strand: Reverse

Name: nuoL [H]

Synonym: Chy400_1925

Alternate gene names: 222525186

Gene position: 2443761-2442178 (Counterclockwise)

Preceding gene: 222525193

Following gene: 222525185

Centisome position: 46.38

GC content: 54.29

Gene sequence:

>1584_bases
ATGGACGTGTTGGTCAGTAACCTGGCGCTGTTGGGGTATCTCCCACCACTGTTGTTGATGGCCGTGGCCTTGCTCGCGGC
CATCAATCTGTTGCCGTCGACTCATCTGACATTTCAGATCGGACGGCTGATCGGGATCGGTTCATTTTTGATCCTCCTCG
GTATTGGGATTGTTGTCTTTACTTCTGGGCCAATAGTAAGCCCAACCATCGGTGTTGGTGAGATCGGGTTTGTCGCCAGG
TTTGACGCACTGAGTGTCGTAATGTCGTGGTTGGTCATCTTGCTGGGTACGCTCTTGATCCAGTTTAGCCGTAATTACCT
CGATGGTGATCCACGCCAGAAGGTGTTTTTTCTTCGTCTCTATCTAACAATTGGTACAGTGTTATTGATGGTATCATCCG
GCAATCTCTGGCAATTGGTGCTGGCCTGGATTGGTACCAGTCTTGCCCTGCACGAATTGCTGGTCTTCTACCCCGAACGC
TCACGCGCCATCCGGGCCGCCCGCAAGAAGTTTGTCGTAGCGCGGATCGGAGACATCTGCCTTATCATTGCGGCAGTTCT
CCTTGCCTCGGATTTTGGCACGACCGACATCGGCATGATCCGGGCTGCCGCTGCCTCAGCTCTTGCCACAGGCGTTATCC
CAACCGGAGCAGCCATCGCTGCCATTTTGATTGTCATAACCGCAGCTTTGAAATCGGCGATGTTTCCCTTCCACGGTTGG
CTGCTTGAGGTGATGGAGACACCTACGCCGGTTTCAGCACTGCTGCACGCCGGTCTGCTGAATGCGGGCATCTTCCTGGT
TGTCCGTTTCGGTGAACTGGTGTTTCTCTCCACCCCGGCACTGATCCTGCTGATCGTGATTGGTGGCTTTACCGCCATCT
TCGCTTCATCGGCAATGATTACTCAGAGCAGCGTGAAGGTCTCGCTGGCCTATTCCAGTGCTGCACACATGGCGTTTATG
CTGATGCTGTGTGGGTTTGGCGCGCACACAGTAGCGATTATGCACCTGATCGCCCACTCGTGTTACAAGGCGCACGCTTT
CCTGTCGTCGGGCAGCATTATTGAATACGTGCGCAATACCGGCATCCAGAAACTCGACAAAGTCCCCAATCCGTTGCAGT
TGCTGGTGAATATCGTGCTGGCAATCGCCATCTTCCTGGCAGTCGCGACAGCACTGGGGATTGATGTCACCAAACGCCCC
GCCGAGACCGCAATGATCACGATCTTTATGATCGCGGTTGCCTATCTGATTGTCAAAGGCACGACCGGCCAGGCCCCGCT
GTACGTTGCGAGTCGCACCATTGGTATGGCAACCCTGGTCACCATCGCCTTTTTCATCCTCGAAGTTGTCGCTGAAGCCA
TATTCGGTTCGGCGGTTGCTGTCTACCCACCACTTGATCCGACTACCTTCACAATGGTGATTGTGTCGGTGATCGCGTTT
GCGTTGGTAATGATCCTCAGCGTCTACCTGCCGATCCTGAAGGATCGTCCGGCCTGGCGCACCTTCTACGTCCATCTCAA
GAATGGTTTCTACGCTAACACGCTGTTTGACCGCTTCATCGATACGTTTCGCCTGGCCCGTTAA

Upstream 100 bases:

>100_bases
TCTTAGTAGAACGTTTTATACAGATACATATATTTTACTGATTATTTAACACATGCTACTATCGAGATGTCGAGTATATT
GTGAAGGAGGGAACGACAGT

Downstream 100 bases:

>100_bases
TCGTTGAATGGGGATTACTCGCTTGCAATGGAGGTAATACCAATGACCATGATTGAAACGCAGCTTCGCTCGCTACTGGT
GACCACGAAGCCAATCCGCG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MDVLVSNLALLGYLPPLLLMAVALLAAINLLPSTHLTFQIGRLIGIGSFLILLGIGIVVFTSGPIVSPTIGVGEIGFVAR
FDALSVVMSWLVILLGTLLIQFSRNYLDGDPRQKVFFLRLYLTIGTVLLMVSSGNLWQLVLAWIGTSLALHELLVFYPER
SRAIRAARKKFVVARIGDICLIIAAVLLASDFGTTDIGMIRAAAASALATGVIPTGAAIAAILIVITAALKSAMFPFHGW
LLEVMETPTPVSALLHAGLLNAGIFLVVRFGELVFLSTPALILLIVIGGFTAIFASSAMITQSSVKVSLAYSSAAHMAFM
LMLCGFGAHTVAIMHLIAHSCYKAHAFLSSGSIIEYVRNTGIQKLDKVPNPLQLLVNIVLAIAIFLAVATALGIDVTKRP
AETAMITIFMIAVAYLIVKGTTGQAPLYVASRTIGMATLVTIAFFILEVVAEAIFGSAVAVYPPLDPTTFTMVIVSVIAF
ALVMILSVYLPILKDRPAWRTFYVHLKNGFYANTLFDRFIDTFRLAR

Sequences:

>Translated_527_residues
MDVLVSNLALLGYLPPLLLMAVALLAAINLLPSTHLTFQIGRLIGIGSFLILLGIGIVVFTSGPIVSPTIGVGEIGFVAR
FDALSVVMSWLVILLGTLLIQFSRNYLDGDPRQKVFFLRLYLTIGTVLLMVSSGNLWQLVLAWIGTSLALHELLVFYPER
SRAIRAARKKFVVARIGDICLIIAAVLLASDFGTTDIGMIRAAAASALATGVIPTGAAIAAILIVITAALKSAMFPFHGW
LLEVMETPTPVSALLHAGLLNAGIFLVVRFGELVFLSTPALILLIVIGGFTAIFASSAMITQSSVKVSLAYSSAAHMAFM
LMLCGFGAHTVAIMHLIAHSCYKAHAFLSSGSIIEYVRNTGIQKLDKVPNPLQLLVNIVLAIAIFLAVATALGIDVTKRP
AETAMITIFMIAVAYLIVKGTTGQAPLYVASRTIGMATLVTIAFFILEVVAEAIFGSAVAVYPPLDPTTFTMVIVSVIAF
ALVMILSVYLPILKDRPAWRTFYVHLKNGFYANTLFDRFIDTFRLAR
>Mature_527_residues
MDVLVSNLALLGYLPPLLLMAVALLAAINLLPSTHLTFQIGRLIGIGSFLILLGIGIVVFTSGPIVSPTIGVGEIGFVAR
FDALSVVMSWLVILLGTLLIQFSRNYLDGDPRQKVFFLRLYLTIGTVLLMVSSGNLWQLVLAWIGTSLALHELLVFYPER
SRAIRAARKKFVVARIGDICLIIAAVLLASDFGTTDIGMIRAAAASALATGVIPTGAAIAAILIVITAALKSAMFPFHGW
LLEVMETPTPVSALLHAGLLNAGIFLVVRFGELVFLSTPALILLIVIGGFTAIFASSAMITQSSVKVSLAYSSAAHMAFM
LMLCGFGAHTVAIMHLIAHSCYKAHAFLSSGSIIEYVRNTGIQKLDKVPNPLQLLVNIVLAIAIFLAVATALGIDVTKRP
AETAMITIFMIAVAYLIVKGTTGQAPLYVASRTIGMATLVTIAFFILEVVAEAIFGSAVAVYPPLDPTTFTMVIVSVIAF
ALVMILSVYLPILKDRPAWRTFYVHLKNGFYANTLFDRFIDTFRLAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=302, Percent_Identity=29.1390728476821, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1788614, Length=340, Percent_Identity=32.9411764705882, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788831, Length=272, Percent_Identity=27.9411764705882, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788829, Length=328, Percent_Identity=27.4390243902439, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56529; Mature: 56529

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLVSNLALLGYLPPLLLMAVALLAAINLLPSTHLTFQIGRLIGIGSFLILLGIGIVVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
TSGPIVSPTIGVGEIGFVARFDALSVVMSWLVILLGTLLIQFSRNYLDGDPRQKVFFLRL
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YLTIGTVLLMVSSGNLWQLVLAWIGTSLALHELLVFYPERSRAIRAARKKFVVARIGDIC
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIIAAVLLASDFGTTDIGMIRAAAASALATGVIPTGAAIAAILIVITAALKSAMFPFHGW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
LLEVMETPTPVSALLHAGLLNAGIFLVVRFGELVFLSTPALILLIVIGGFTAIFASSAMI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHE
TQSSVKVSLAYSSAAHMAFMLMLCGFGAHTVAIMHLIAHSCYKAHAFLSSGSIIEYVRNT
ECCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
GIQKLDKVPNPLQLLVNIVLAIAIFLAVATALGIDVTKRPAETAMITIFMIAVAYLIVKG
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TTGQAPLYVASRTIGMATLVTIAFFILEVVAEAIFGSAVAVYPPLDPTTFTMVIVSVIAF
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
ALVMILSVYLPILKDRPAWRTFYVHLKNGFYANTLFDRFIDTFRLAR
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVLVSNLALLGYLPPLLLMAVALLAAINLLPSTHLTFQIGRLIGIGSFLILLGIGIVVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
TSGPIVSPTIGVGEIGFVARFDALSVVMSWLVILLGTLLIQFSRNYLDGDPRQKVFFLRL
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YLTIGTVLLMVSSGNLWQLVLAWIGTSLALHELLVFYPERSRAIRAARKKFVVARIGDIC
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIIAAVLLASDFGTTDIGMIRAAAASALATGVIPTGAAIAAILIVITAALKSAMFPFHGW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
LLEVMETPTPVSALLHAGLLNAGIFLVVRFGELVFLSTPALILLIVIGGFTAIFASSAMI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHE
TQSSVKVSLAYSSAAHMAFMLMLCGFGAHTVAIMHLIAHSCYKAHAFLSSGSIIEYVRNT
ECCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
GIQKLDKVPNPLQLLVNIVLAIAIFLAVATALGIDVTKRPAETAMITIFMIAVAYLIVKG
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TTGQAPLYVASRTIGMATLVTIAFFILEVVAEAIFGSAVAVYPPLDPTTFTMVIVSVIAF
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
ALVMILSVYLPILKDRPAWRTFYVHLKNGFYANTLFDRFIDTFRLAR
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]