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The map label for this gene is bphD [H]

Identifier: 222525142

GI number: 222525142

Start: 2374857

End: 2375708

Strand: Reverse

Name: bphD [H]

Synonym: Chy400_1880

Alternate gene names: 222525142

Gene position: 2375708-2374857 (Counterclockwise)

Preceding gene: 222525154

Following gene: 222525138

Centisome position: 45.09

GC content: 56.57

Gene sequence:

>852_bases
ATGCCAGCAACAACTGAGGTGGCGATTACTGATCAACATGTGGTTATTGATGGATTTCGCTTGCGTGTGTTGACCGCCGG
TCAGGGGCCAGCCGTGCTCTTGCTGCACGGCTTCGTCGTGAGTGCTGACGACTGGATTCCGACCATTCGTCTGTTAGCGG
CGAGTGGTTTTCAGGCGATTGCTCCCGATGCGTTAGGATTTGGTGCCTCAGATAAGCCAGGCGGTGCCGTCTACACGTTG
CGTCGATATGCCGATCTCAATGCAGGGTTACTGCGCTTCTTCGCGGTTGATCAGGCAGCGGTTGTCGGTCATTCAATGGG
CGGGAAACATGCGCTGGCAACGACTATCCTCTACCCACAGCAGGTTTCGCGCTTAGTCATTGCCGATAGCGAAGGCTTCA
TGCAGCTTCCATTGTTTATGCGCAAAGGGGGAGCAATACCATTCCTCGGTGAAGCGCTGGTCGGGTTGTCGGCGCTGCCC
TTTGTGGTGCGGATGCAGTTACGAACGGCGTTTGCCAATCCTGATCGCTACATTACCCCTGATCTGATCGCTCGTGGCAC
AGCAACCCTGGGTAATCCTGAGATTCGACGCACCATGGTGGCGCTGAGTCGCTATTTTGATGCCAACGATCTGCGCGGGA
GTGGTCTCTGGCCGCGACTTGCCGACATCCGGCAGCCGACGCTCATCATGTGGGGGGCTGAAGATCGGCTCTTCCCGGTA
CGCTACGCCTACGAAGCGCAACGTGCTCTCCCGCACGCCCGACTGGAGATTATTCCGAATTGCGGTCATTTTCCGATGAT
CGAAGCGGCAGATCGCTTCCATCAGCTCTTGCTCGAATTTTTGCGCCAGTGA

Upstream 100 bases:

>100_bases
GTCGGGTCTTGTCCTGACATTGCGAGCGTTATTTGCTAGAGTGAGATCGTGACAAAGAGCAGTTCGTATTCTCCGCTTTG
CAAAGAGGAGTATCGAGTGT

Downstream 100 bases:

>100_bases
TCAGGTACCCGGCAACCTGTGATTATCCGACTATGATCAATACCTGCCGCATCTTTACGGGAAAACACAGGACTCGGCCT
GTGTTAACCCTCTGTTACCT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: HOPDA hydrolase; 2,6-dioxo-6-phenylhexa-3-enoate hydrolase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPATTEVAITDQHVVIDGFRLRVLTAGQGPAVLLLHGFVVSADDWIPTIRLLAASGFQAIAPDALGFGASDKPGGAVYTL
RRYADLNAGLLRFFAVDQAAVVGHSMGGKHALATTILYPQQVSRLVIADSEGFMQLPLFMRKGGAIPFLGEALVGLSALP
FVVRMQLRTAFANPDRYITPDLIARGTATLGNPEIRRTMVALSRYFDANDLRGSGLWPRLADIRQPTLIMWGAEDRLFPV
RYAYEAQRALPHARLEIIPNCGHFPMIEAADRFHQLLLEFLRQ

Sequences:

>Translated_283_residues
MPATTEVAITDQHVVIDGFRLRVLTAGQGPAVLLLHGFVVSADDWIPTIRLLAASGFQAIAPDALGFGASDKPGGAVYTL
RRYADLNAGLLRFFAVDQAAVVGHSMGGKHALATTILYPQQVSRLVIADSEGFMQLPLFMRKGGAIPFLGEALVGLSALP
FVVRMQLRTAFANPDRYITPDLIARGTATLGNPEIRRTMVALSRYFDANDLRGSGLWPRLADIRQPTLIMWGAEDRLFPV
RYAYEAQRALPHARLEIIPNCGHFPMIEAADRFHQLLLEFLRQ
>Mature_282_residues
PATTEVAITDQHVVIDGFRLRVLTAGQGPAVLLLHGFVVSADDWIPTIRLLAASGFQAIAPDALGFGASDKPGGAVYTLR
RYADLNAGLLRFFAVDQAAVVGHSMGGKHALATTILYPQQVSRLVIADSEGFMQLPLFMRKGGAIPFLGEALVGLSALPF
VVRMQLRTAFANPDRYITPDLIARGTATLGNPEIRRTMVALSRYFDANDLRGSGLWPRLADIRQPTLIMWGAEDRLFPVR
YAYEAQRALPHARLEIIPNCGHFPMIEAADRFHQLLLEFLRQ

Specific function: Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6- oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. BphD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=277, Percent_Identity=31.7689530685921, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR017727 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 31047; Mature: 30915

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATTEVAITDQHVVIDGFRLRVLTAGQGPAVLLLHGFVVSADDWIPTIRLLAASGFQAI
CCCCCEEEEECCEEEEECEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCC
APDALGFGASDKPGGAVYTLRRYADLNAGLLRFFAVDQAAVVGHSMGGKHALATTILYPQ
CCHHHCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHEEECHH
QVSRLVIADSEGFMQLPLFMRKGGAIPFLGEALVGLSALPFVVRMQLRTAFANPDRYITP
HHCEEEEECCCCCEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
DLIARGTATLGNPEIRRTMVALSRYFDANDLRGSGLWPRLADIRQPTLIMWGAEDRLFPV
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEE
RYAYEAQRALPHARLEIIPNCGHFPMIEAADRFHQLLLEFLRQ
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PATTEVAITDQHVVIDGFRLRVLTAGQGPAVLLLHGFVVSADDWIPTIRLLAASGFQAI
CCCCEEEEECCEEEEECEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCC
APDALGFGASDKPGGAVYTLRRYADLNAGLLRFFAVDQAAVVGHSMGGKHALATTILYPQ
CCHHHCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHEEECHH
QVSRLVIADSEGFMQLPLFMRKGGAIPFLGEALVGLSALPFVVRMQLRTAFANPDRYITP
HHCEEEEECCCCCEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
DLIARGTATLGNPEIRRTMVALSRYFDANDLRGSGLWPRLADIRQPTLIMWGAEDRLFPV
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEE
RYAYEAQRALPHARLEIIPNCGHFPMIEAADRFHQLLLEFLRQ
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA