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The map label for this gene is ycbU [H]

Identifier: 222525154

GI number: 222525154

Start: 2392811

End: 2393953

Strand: Reverse

Name: ycbU [H]

Synonym: Chy400_1892

Alternate gene names: 222525154

Gene position: 2393953-2392811 (Counterclockwise)

Preceding gene: 222525155

Following gene: 222525142

Centisome position: 45.44

GC content: 57.57

Gene sequence:

>1143_bases
ATGACTGTCGATTTGGCCGCTTATCGCGCTGGATTTCCGATTACCGAACGCTACGCCTTTCTTAGCCACGCGGCTGTCTC
ACCGTTGCATCAGCATGTGCAGGCAGCAGTGCAGAGCTACCTTGACCATGCCACCCGTGAGCCGTTTCTGGCCGTCTTTC
CACAGGTGATGGCACAGTTTGCCGAGCTTAAGCAGCGCCTGGCCCGGCTGATCAACGCCCGCTCGCCCGACGAGATAGTG
CTAATGCCCAATACGGCTGCCGGCATTAACACCGCAGCCGTTAGCCTGCCGTTACACCCCGGTGATAATGTGCTGGTGCT
TGATGGTGACTATCCGGCTAACATCTACCCGTGGCAGCAACTGGCATATCGTGGTGTGCTAACAAAGGTCGTGCCGCAAC
ATCAGGGTGGCCTCGATCTCGATCTGCTGGTGCGACGCATTGATCATCGGACGCGGGTGATCGCGCTTAGCTCAGCGATG
TTTGCGACCGGGTTTCGCAATGATCTGGCGACCGTCGGCCAGCTTTGTCGCGAGCGAGGCATCTATTTTGTGGTTGATGC
GATCCAGACACTGGGGGCATTTCCGCTCGATGTGCAGGAATGCCATATCGACATGCTGGCATGTGGTTCGCAAAAGTGGT
TGCTATCAACACCCGGCAGTGGCTTCCTCTACGTGCGCCGTGAGTTGATTCGCGATCTGGTGCCAGGAGCTTATGTCGGC
GCCGCCAGTAGTGTGAGCGGGATGAACTATCTCGACTACAATCTAACCCTGCCTGAAACTGCCGAGCGGTTTACACTGGG
AACACCAAATGTCGCCAACAATCTCGCCCTATTGGCTGCGGTTACGTTGCTGCAAGAGGTTGGCATTGAGCGGATCACGC
ACCAGATTGCGACGCTGGTGGATGCGTTGATCAACGATCTCCAGGAGCGCGGCTATCAACTGGCTGCAAGTACCAAACCA
GAACACCGGAGTGGCATTGTGGTCGCGATGGTCGAGAATCCGGCTGTGGTCGCACAACGCCTCTACGAGGCCGGGATTGT
TGTTACTCCGCGGGGAGCCGGCGTGCGGATCGCCCCCCACTTCTACAATACGCTCGATGAGGTGTTGCGGGTCGGTGAAG
CACTTGATGCGGCAACCCACTGA

Upstream 100 bases:

>100_bases
GCCGGCTGGTTTGGATTGAGCAGTGTGGTCATTTGCCAATGCTGGAACGACCGCACGAATACCACGCTATTCTGACCGAA
TTTCTGAAGGATGGGGCTGT

Downstream 100 bases:

>100_bases
GCGGCAGACAGGCCATCCGATACGTTTATACAGCCTTAGTGCTTCTCATCAACCACTTCGACACCGATAGCACGCAGACG
GTTCAGGATCGTCTGCGTGC

Product: class V aminotransferase

Products: hydrogen selenide; @ALAN01.txt*l-alanine!; acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTVDLAAYRAGFPITERYAFLSHAAVSPLHQHVQAAVQSYLDHATREPFLAVFPQVMAQFAELKQRLARLINARSPDEIV
LMPNTAAGINTAAVSLPLHPGDNVLVLDGDYPANIYPWQQLAYRGVLTKVVPQHQGGLDLDLLVRRIDHRTRVIALSSAM
FATGFRNDLATVGQLCRERGIYFVVDAIQTLGAFPLDVQECHIDMLACGSQKWLLSTPGSGFLYVRRELIRDLVPGAYVG
AASSVSGMNYLDYNLTLPETAERFTLGTPNVANNLALLAAVTLLQEVGIERITHQIATLVDALINDLQERGYQLAASTKP
EHRSGIVVAMVENPAVVAQRLYEAGIVVTPRGAGVRIAPHFYNTLDEVLRVGEALDAATH

Sequences:

>Translated_380_residues
MTVDLAAYRAGFPITERYAFLSHAAVSPLHQHVQAAVQSYLDHATREPFLAVFPQVMAQFAELKQRLARLINARSPDEIV
LMPNTAAGINTAAVSLPLHPGDNVLVLDGDYPANIYPWQQLAYRGVLTKVVPQHQGGLDLDLLVRRIDHRTRVIALSSAM
FATGFRNDLATVGQLCRERGIYFVVDAIQTLGAFPLDVQECHIDMLACGSQKWLLSTPGSGFLYVRRELIRDLVPGAYVG
AASSVSGMNYLDYNLTLPETAERFTLGTPNVANNLALLAAVTLLQEVGIERITHQIATLVDALINDLQERGYQLAASTKP
EHRSGIVVAMVENPAVVAQRLYEAGIVVTPRGAGVRIAPHFYNTLDEVLRVGEALDAATH
>Mature_379_residues
TVDLAAYRAGFPITERYAFLSHAAVSPLHQHVQAAVQSYLDHATREPFLAVFPQVMAQFAELKQRLARLINARSPDEIVL
MPNTAAGINTAAVSLPLHPGDNVLVLDGDYPANIYPWQQLAYRGVLTKVVPQHQGGLDLDLLVRRIDHRTRVIALSSAMF
ATGFRNDLATVGQLCRERGIYFVVDAIQTLGAFPLDVQECHIDMLACGSQKWLLSTPGSGFLYVRRELIRDLVPGAYVGA
ASSVSGMNYLDYNLTLPETAERFTLGTPNVANNLALLAAVTLLQEVGIERITHQIATLVDALINDLQERGYQLAASTKPE
HRSGIVVAMVENPAVVAQRLYEAGIVVTPRGAGVRIAPHFYNTLDEVLRVGEALDAATH

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=322, Percent_Identity=28.2608695652174, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789175, Length=399, Percent_Identity=28.8220551378446, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994898, Length=169, Percent_Identity=28.4023668639053, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=169, Percent_Identity=27.2189349112426, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129463, Length=218, Percent_Identity=26.1467889908257, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.8.1.7 [C]

Molecular weight: Translated: 41480; Mature: 41349

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDLAAYRAGFPITERYAFLSHAAVSPLHQHVQAAVQSYLDHATREPFLAVFPQVMAQF
CCEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AELKQRLARLINARSPDEIVLMPNTAAGINTAAVSLPLHPGDNVLVLDGDYPANIYPWQQ
HHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCHHH
LAYRGVLTKVVPQHQGGLDLDLLVRRIDHRTRVIALSSAMFATGFRNDLATVGQLCRERG
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHCC
IYFVVDAIQTLGAFPLDVQECHIDMLACGSQKWLLSTPGSGFLYVRRELIRDLVPGAYVG
CEEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHC
AASSVSGMNYLDYNLTLPETAERFTLGTPNVANNLALLAAVTLLQEVGIERITHQIATLV
CCCCCCCCEEEEEECCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALINDLQERGYQLAASTKPEHRSGIVVAMVENPAVVAQRLYEAGIVVTPRGAGVRIAPH
HHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEECCCCCEEECHH
FYNTLDEVLRVGEALDAATH
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVDLAAYRAGFPITERYAFLSHAAVSPLHQHVQAAVQSYLDHATREPFLAVFPQVMAQF
CEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AELKQRLARLINARSPDEIVLMPNTAAGINTAAVSLPLHPGDNVLVLDGDYPANIYPWQQ
HHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCHHH
LAYRGVLTKVVPQHQGGLDLDLLVRRIDHRTRVIALSSAMFATGFRNDLATVGQLCRERG
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHCC
IYFVVDAIQTLGAFPLDVQECHIDMLACGSQKWLLSTPGSGFLYVRRELIRDLVPGAYVG
CEEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHC
AASSVSGMNYLDYNLTLPETAERFTLGTPNVANNLALLAAVTLLQEVGIERITHQIATLV
CCCCCCCCEEEEEECCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALINDLQERGYQLAASTKPEHRSGIVVAMVENPAVVAQRLYEAGIVVTPRGAGVRIAPH
HHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEECCCCCEEECHH
FYNTLDEVLRVGEALDAATH
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-selenocysteine; reduced acceptor [C]

Specific reaction: L-selenocysteine + reduced acceptor = hydrogen selenide + @ALAN01.txt*l-alanine! + acceptor [C]

General reaction: Elimination of H2S or RSH [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]