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The map label for this gene is rpfG [H]

Identifier: 222525136

GI number: 222525136

Start: 2368688

End: 2370412

Strand: Direct

Name: rpfG [H]

Synonym: Chy400_1874

Alternate gene names: 222525136

Gene position: 2368688-2370412 (Clockwise)

Preceding gene: 222525135

Following gene: 222525137

Centisome position: 44.96

GC content: 54.43

Gene sequence:

>1725_bases
ATGGGTCAAGAGGGGGAACTGGTTATGAACGATCAAGAACAAGCACCTAAACGCTACTTAGAGATTATCCAACGTGCCAA
TCAGATTGCTGCCACAACCCAACTTGATGATCTCCTTGATCGTTTGCTCGATCTCATTATCGAGATCACGCAATCAGAAG
CCGGTACCCTCTACCTGTACGATGCCGCAACCGATGAACTCATCTTCAAAGTCGTAAAAGGCAGTCCTGCCGGCGAGGCG
CTGCTCGGTCGCCGCCTGCCGGCCAATACCGGATTGGCCGGTTATGCCTTGCAACATCGGCAACCGTTTTTCGTCAACGA
TGTCGCGAATGATCCACGCTGGAATCGAACCTTCGGCGAAATGGGTTCACTCGATCTGCGCACGATGTTCTGTGTACCTT
TGCTGTTACGGGGCGAACCGGTGGGCGTGGTGCAGGTCTTCAATGGCGCGTTTGAGGCGGTTGATGAACAGGAAGAGCTG
CTTCTGATCGATTTGCTTACGTCACGGCTGGTGACTGAAATCGAAAAAGCCCGGTTGTTAGAGGAGTCGCAACAGCGCGA
ACGCCGCCAGCATGCGCTGGTCGAGATCGTCTCACATTTGACGACCACCCTGGAACGTGATGAGTTGTTACGCCGGATCA
TGAGTTACGCCTGCGAACTGCTTGAGGTCGAAGCGGCATCGATCTGGCTGATCGATCATGAGCGAAACGATCTGGTCTTG
CACATTGCCGGCGGTGAAAACAGCGAGCGCGTAGAGTCGTTACGTGTGCCACGCGGACAGGGCATCATCGGTCACGTGAT
TGAAACCGGTGAAACAGTTGTCGTTAATGATGTCCAGCGCGATCATCGCTTCTACCAGGGACTTGATCAACGGAGCGGAT
TTCAGACCCGTGCCATTCTCTGTGTACCGTTGCGCTCGCCCCAAATCATTCTTGGTGGTGAACGTGGTGAAGTGGAGGGG
ATGATCATCGGTGGGTCGCAAGCCCTCAACCCAAAGCACGGTCGAACATTTTCTAACGACGATATTGTTCTGTTTCAAAC
CCTTGCGTCACAGGCAGCAACGGTGATTCGGCTGGCTGAACTGTATCGAGAGACGGATAGCCTGTTTACCCGGATTATTG
CCGCCATCACGGGGGCGATTGATCTTAAAGACCCGTACACTCGTGGACACAGTCAGCGGGTCAGCGATTTTTCGGTAGCC
ATTGCCCAAGAGCTAGGGCTATCACGGGAACAGATTTACCAGTTGCGCATTGCCAGTAAGTTGCACGATGTTGGCAAGAT
TCGTGTTCCTGACCATATCCTCAAGAAACCGGGTCGGCTTGACGAGGAAGAGTTTGCCGAGATGCGCCGGCATCCTCTCT
ACGGCATCGAATTTCTCCGCGATAATGGGTTGCTCGAACTCGATCTGCTGCGCGAGTCGTGGACGGCACTGGCTCAGCAC
CACGAACGGCTTGATGGCCGTGGCTATCCATACGGTCTGAAGGGTGAAGATATTTCACTCTTTGGCCGCATTGTCGCGGT
TGCCGATGTGTTCGATGCGATTACCAGCCATCGACCATATCGGCCCCCAATGCCCATCGACGAAGCCCTGGCACTTTTGC
AGCGCGGTGCCGGTACGGAGTTCGATCCGGCATGTGTGGAAGCGCTGATTCGGGCACGGGCGAAAGGGGCTATCCTGACC
CAAGATGAACGTGAACAGGCAATTGCATCGCAGACGATGGAGTGA

Upstream 100 bases:

>100_bases
CGGTGAGGCCTCTGAACTGGTGCGCTACGTACAGGCATTGGCCCGCAGTGACCAGGAGTAGTAGGTGCTGGTCACATAAG
TTCTCGCGTGCATCTCAGCT

Downstream 100 bases:

>100_bases
TAATTATGCGCGTTCTCCTGATCGACAACTACGACTCGTTTACATACAATCTGTACCAGTATCTTTGTGAACTGGGCGCT
GACGTTGAGGTGGTGCGGAA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MGQEGELVMNDQEQAPKRYLEIIQRANQIAATTQLDDLLDRLLDLIIEITQSEAGTLYLYDAATDELIFKVVKGSPAGEA
LLGRRLPANTGLAGYALQHRQPFFVNDVANDPRWNRTFGEMGSLDLRTMFCVPLLLRGEPVGVVQVFNGAFEAVDEQEEL
LLIDLLTSRLVTEIEKARLLEESQQRERRQHALVEIVSHLTTTLERDELLRRIMSYACELLEVEAASIWLIDHERNDLVL
HIAGGENSERVESLRVPRGQGIIGHVIETGETVVVNDVQRDHRFYQGLDQRSGFQTRAILCVPLRSPQIILGGERGEVEG
MIIGGSQALNPKHGRTFSNDDIVLFQTLASQAATVIRLAELYRETDSLFTRIIAAITGAIDLKDPYTRGHSQRVSDFSVA
IAQELGLSREQIYQLRIASKLHDVGKIRVPDHILKKPGRLDEEEFAEMRRHPLYGIEFLRDNGLLELDLLRESWTALAQH
HERLDGRGYPYGLKGEDISLFGRIVAVADVFDAITSHRPYRPPMPIDEALALLQRGAGTEFDPACVEALIRARAKGAILT
QDEREQAIASQTME

Sequences:

>Translated_574_residues
MGQEGELVMNDQEQAPKRYLEIIQRANQIAATTQLDDLLDRLLDLIIEITQSEAGTLYLYDAATDELIFKVVKGSPAGEA
LLGRRLPANTGLAGYALQHRQPFFVNDVANDPRWNRTFGEMGSLDLRTMFCVPLLLRGEPVGVVQVFNGAFEAVDEQEEL
LLIDLLTSRLVTEIEKARLLEESQQRERRQHALVEIVSHLTTTLERDELLRRIMSYACELLEVEAASIWLIDHERNDLVL
HIAGGENSERVESLRVPRGQGIIGHVIETGETVVVNDVQRDHRFYQGLDQRSGFQTRAILCVPLRSPQIILGGERGEVEG
MIIGGSQALNPKHGRTFSNDDIVLFQTLASQAATVIRLAELYRETDSLFTRIIAAITGAIDLKDPYTRGHSQRVSDFSVA
IAQELGLSREQIYQLRIASKLHDVGKIRVPDHILKKPGRLDEEEFAEMRRHPLYGIEFLRDNGLLELDLLRESWTALAQH
HERLDGRGYPYGLKGEDISLFGRIVAVADVFDAITSHRPYRPPMPIDEALALLQRGAGTEFDPACVEALIRARAKGAILT
QDEREQAIASQTME
>Mature_573_residues
GQEGELVMNDQEQAPKRYLEIIQRANQIAATTQLDDLLDRLLDLIIEITQSEAGTLYLYDAATDELIFKVVKGSPAGEAL
LGRRLPANTGLAGYALQHRQPFFVNDVANDPRWNRTFGEMGSLDLRTMFCVPLLLRGEPVGVVQVFNGAFEAVDEQEELL
LIDLLTSRLVTEIEKARLLEESQQRERRQHALVEIVSHLTTTLERDELLRRIMSYACELLEVEAASIWLIDHERNDLVLH
IAGGENSERVESLRVPRGQGIIGHVIETGETVVVNDVQRDHRFYQGLDQRSGFQTRAILCVPLRSPQIILGGERGEVEGM
IIGGSQALNPKHGRTFSNDDIVLFQTLASQAATVIRLAELYRETDSLFTRIIAAITGAIDLKDPYTRGHSQRVSDFSVAI
AQELGLSREQIYQLRIASKLHDVGKIRVPDHILKKPGRLDEEEFAEMRRHPLYGIEFLRDNGLLELDLLRESWTALAQHH
ERLDGRGYPYGLKGEDISLFGRIVAVADVFDAITSHRPYRPPMPIDEALALLQRGAGTEFDPACVEALIRARAKGAILTQ
DEREQAIASQTME

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Homo sapiens, GI5729972, Length=386, Percent_Identity=24.3523316062176, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI194595488, Length=386, Percent_Identity=24.3523316062176, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI4505657, Length=331, Percent_Identity=25.3776435045317, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI226246591, Length=331, Percent_Identity=25.3776435045317, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI219802706, Length=331, Percent_Identity=25.3776435045317, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI116536085, Length=364, Percent_Identity=24.7252747252747, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI61744430, Length=180, Percent_Identity=29.4444444444444, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI61744432, Length=180, Percent_Identity=29.4444444444444, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI61744435, Length=180, Percent_Identity=29.4444444444444, Blast_Score=71, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193202333, Length=165, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161076955, Length=303, Percent_Identity=26.4026402640264, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI161076953, Length=303, Percent_Identity=26.4026402640264, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 64425; Mature: 64294

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQEGELVMNDQEQAPKRYLEIIQRANQIAATTQLDDLLDRLLDLIIEITQSEAGTLYLY
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DAATDELIFKVVKGSPAGEALLGRRLPANTGLAGYALQHRQPFFVNDVANDPRWNRTFGE
ECCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHC
MGSLDLRTMFCVPLLLRGEPVGVVQVFNGAFEAVDEQEELLLIDLLTSRLVTEIEKARLL
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EESQQRERRQHALVEIVSHLTTTLERDELLRRIMSYACELLEVEAASIWLIDHERNDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEEEECCCCEEE
HIAGGENSERVESLRVPRGQGIIGHVIETGETVVVNDVQRDHRFYQGLDQRSGFQTRAIL
EEECCCCHHHHHHHCCCCCCCCHHHHCCCCCEEEEECHHHHHHHHHCHHHCCCCCEEEEE
CVPLRSPQIILGGERGEVEGMIIGGSQALNPKHGRTFSNDDIVLFQTLASQAATVIRLAE
EEECCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
LYRETDSLFTRIIAAITGAIDLKDPYTRGHSQRVSDFSVAIAQELGLSREQIYQLRIASK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LHDVGKIRVPDHILKKPGRLDEEEFAEMRRHPLYGIEFLRDNGLLELDLLRESWTALAQH
HHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHH
HERLDGRGYPYGLKGEDISLFGRIVAVADVFDAITSHRPYRPPMPIDEALALLQRGAGTE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC
FDPACVEALIRARAKGAILTQDEREQAIASQTME
CCHHHHHHHHHHHHCCCEECCHHHHHHHHHHCCC
>Mature Secondary Structure 
GQEGELVMNDQEQAPKRYLEIIQRANQIAATTQLDDLLDRLLDLIIEITQSEAGTLYLY
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DAATDELIFKVVKGSPAGEALLGRRLPANTGLAGYALQHRQPFFVNDVANDPRWNRTFGE
ECCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCHHC
MGSLDLRTMFCVPLLLRGEPVGVVQVFNGAFEAVDEQEELLLIDLLTSRLVTEIEKARLL
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EESQQRERRQHALVEIVSHLTTTLERDELLRRIMSYACELLEVEAASIWLIDHERNDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEEEECCCCEEE
HIAGGENSERVESLRVPRGQGIIGHVIETGETVVVNDVQRDHRFYQGLDQRSGFQTRAIL
EEECCCCHHHHHHHCCCCCCCCHHHHCCCCCEEEEECHHHHHHHHHCHHHCCCCCEEEEE
CVPLRSPQIILGGERGEVEGMIIGGSQALNPKHGRTFSNDDIVLFQTLASQAATVIRLAE
EEECCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
LYRETDSLFTRIIAAITGAIDLKDPYTRGHSQRVSDFSVAIAQELGLSREQIYQLRIASK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LHDVGKIRVPDHILKKPGRLDEEEFAEMRRHPLYGIEFLRDNGLLELDLLRESWTALAQH
HHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHH
HERLDGRGYPYGLKGEDISLFGRIVAVADVFDAITSHRPYRPPMPIDEALALLQRGAGTE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC
FDPACVEALIRARAKGAILTQDEREQAIASQTME
CCHHHHHHHHHHHHCCCEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]