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The map label for this gene is bfmBAB [H]

Identifier: 222525095

GI number: 222525095

Start: 2321326

End: 2322309

Strand: Reverse

Name: bfmBAB [H]

Synonym: Chy400_1833

Alternate gene names: 222525095

Gene position: 2322309-2321326 (Counterclockwise)

Preceding gene: 222525096

Following gene: 222525094

Centisome position: 44.08

GC content: 56.71

Gene sequence:

>984_bases
ATGCCTGAGATGAACTTGCTGGAAGCTATTCGCCAGGGCCTCGATGAAGCCATGGCTGCCGATTCACGGGTATTCATTTT
CGGTGAAGATGTGGGCAAACGCGGCGGTGTCTTCCGTGTCACCGAAGGTTTGTACGACAAGTATGGCCCCATGCGCGTCA
TTGACTCGCCGCTGGCCGAGAGTGTCATTGTAGGCGCCTGTATCGGCGCCGCAATGAACGATACATTGCCCATCGCCGAG
ATTCAGTTTGCCGATTTTATTGCCCCAGCTTTTAATCAGATCGTGCAGGAAGCGGCCCGTATTCACTACCGCTCTAATGG
TGACTGGGAAGTACCGCTGGTCATTCGGGTGCCTTACGGCGGCGGTATTCACGGGGCACTCTACCATAGCCAAAGCGTGG
AAGCCTTCTTCGCCCACGTGCCCGGCCTGAAGGTCGTGACCCCGGCTACCCCTTACGATGCAAAAGGGTTGCTGAAGAGC
GCGATTGAAGACCCCAATCCAGTCCTCTTCCTCGAACACAAGAAGACCTACCGGCTGATTAAGGGGTTTGTGCCCGAAGA
AGATTATCGAGTGCCGATTGGCCCCGCCGACATCAAACGTCCCGGTGAAGACATGTCAGTCTTTGCCTACGGACTGATGC
TGCACTACTGCCTCGAAGCAGCCCAAACCCTGGCCGCAGAAGGGGTGAGCGTAGAGGTTGTCGATCTACGCACCCTGCGA
CCACTCGATACCGAAACGATCCTCGCCAGTGTCCGCCGTACCGGCAAGGTCTTGATCGTCCACGAGGATAATCTGTTTGG
CGGTTTTGGTGGTGAAGTAGCAGCGATCATTGCCGAACACGCATTTGAGTATCTCGATGGGCCAATTGTGCGTATCGGTG
GCCCTGATGTGCCGGCGATGCCCTTTGCTCATTCGCTGGAAGCGGCGTTTATGCCCTCTCCGGCCTCGATTGCAGCAGCA
ATGCGGCGGCTGGCCGCTTACTGA

Upstream 100 bases:

>100_bases
CGAAGAGGCCGAGCGAGCACCGATGCCAGAACCGGAAACCCTCTACGACCACGTCTACGCGCCGTTGCGCCAGCGTCGCT
GATATGCGGGGAGGATAAAT

Downstream 100 bases:

>100_bases
TGGCTGTCATTAGTCCTGCGACTGATTGGAGAGTGGACACATTCTTTCGTGAGTTACGTAGCAGGTGAGCTTCATGATCG
ATATCAAAATGCCTCAGCTC

Product: transketolase central subunit

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPEMNLLEAIRQGLDEAMAADSRVFIFGEDVGKRGGVFRVTEGLYDKYGPMRVIDSPLAESVIVGACIGAAMNDTLPIAE
IQFADFIAPAFNQIVQEAARIHYRSNGDWEVPLVIRVPYGGGIHGALYHSQSVEAFFAHVPGLKVVTPATPYDAKGLLKS
AIEDPNPVLFLEHKKTYRLIKGFVPEEDYRVPIGPADIKRPGEDMSVFAYGLMLHYCLEAAQTLAAEGVSVEVVDLRTLR
PLDTETILASVRRTGKVLIVHEDNLFGGFGGEVAAIIAEHAFEYLDGPIVRIGGPDVPAMPFAHSLEAAFMPSPASIAAA
MRRLAAY

Sequences:

>Translated_327_residues
MPEMNLLEAIRQGLDEAMAADSRVFIFGEDVGKRGGVFRVTEGLYDKYGPMRVIDSPLAESVIVGACIGAAMNDTLPIAE
IQFADFIAPAFNQIVQEAARIHYRSNGDWEVPLVIRVPYGGGIHGALYHSQSVEAFFAHVPGLKVVTPATPYDAKGLLKS
AIEDPNPVLFLEHKKTYRLIKGFVPEEDYRVPIGPADIKRPGEDMSVFAYGLMLHYCLEAAQTLAAEGVSVEVVDLRTLR
PLDTETILASVRRTGKVLIVHEDNLFGGFGGEVAAIIAEHAFEYLDGPIVRIGGPDVPAMPFAHSLEAAFMPSPASIAAA
MRRLAAY
>Mature_326_residues
PEMNLLEAIRQGLDEAMAADSRVFIFGEDVGKRGGVFRVTEGLYDKYGPMRVIDSPLAESVIVGACIGAAMNDTLPIAEI
QFADFIAPAFNQIVQEAARIHYRSNGDWEVPLVIRVPYGGGIHGALYHSQSVEAFFAHVPGLKVVTPATPYDAKGLLKSA
IEDPNPVLFLEHKKTYRLIKGFVPEEDYRVPIGPADIKRPGEDMSVFAYGLMLHYCLEAAQTLAAEGVSVEVVDLRTLRP
LDTETILASVRRTGKVLIVHEDNLFGGFGGEVAAIIAEHAFEYLDGPIVRIGGPDVPAMPFAHSLEAAFMPSPASIAAAM
RRLAAY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=328, Percent_Identity=47.8658536585366, Blast_Score=286, Evalue=1e-77,
Organism=Homo sapiens, GI34101272, Length=328, Percent_Identity=47.8658536585366, Blast_Score=286, Evalue=1e-77,
Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=41.8461538461538, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=40, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1786622, Length=285, Percent_Identity=22.1052631578947, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17506935, Length=328, Percent_Identity=42.9878048780488, Blast_Score=251, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17538422, Length=324, Percent_Identity=41.0493827160494, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=37.8048780487805, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI160714832, Length=327, Percent_Identity=44.0366972477064, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI160714828, Length=327, Percent_Identity=44.0366972477064, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI21358145, Length=314, Percent_Identity=42.3566878980892, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24650940, Length=314, Percent_Identity=42.3566878980892, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=38.8888888888889, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=38.8888888888889, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 35465; Mature: 35334

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEMNLLEAIRQGLDEAMAADSRVFIFGEDVGKRGGVFRVTEGLYDKYGPMRVIDSPLAE
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECHHHHHCCCCHHHHCCHHHH
SVIVGACIGAAMNDTLPIAEIQFADFIAPAFNQIVQEAARIHYRSNGDWEVPLVIRVPYG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEECCC
GGIHGALYHSQSVEAFFAHVPGLKVVTPATPYDAKGLLKSAIEDPNPVLFLEHKKTYRLI
CCCCHHHCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGFVPEEDYRVPIGPADIKRPGEDMSVFAYGLMLHYCLEAAQTLAAEGVSVEVVDLRTLR
HCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
PLDTETILASVRRTGKVLIVHEDNLFGGFGGEVAAIIAEHAFEYLDGPIVRIGGPDVPAM
CCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
PFAHSLEAAFMPSPASIAAAMRRLAAY
CCHHCCCHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PEMNLLEAIRQGLDEAMAADSRVFIFGEDVGKRGGVFRVTEGLYDKYGPMRVIDSPLAE
CCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECHHHHHCCCCHHHHCCHHHH
SVIVGACIGAAMNDTLPIAEIQFADFIAPAFNQIVQEAARIHYRSNGDWEVPLVIRVPYG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEECCC
GGIHGALYHSQSVEAFFAHVPGLKVVTPATPYDAKGLLKSAIEDPNPVLFLEHKKTYRLI
CCCCHHHCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGFVPEEDYRVPIGPADIKRPGEDMSVFAYGLMLHYCLEAAQTLAAEGVSVEVVDLRTLR
HCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
PLDTETILASVRRTGKVLIVHEDNLFGGFGGEVAAIIAEHAFEYLDGPIVRIGGPDVPAM
CCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
PFAHSLEAAFMPSPASIAAAMRRLAAY
CCHHCCCHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]