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The map label for this gene is bfmBB [H]

Identifier: 222525094

GI number: 222525094

Start: 2319933

End: 2321252

Strand: Reverse

Name: bfmBB [H]

Synonym: Chy400_1832

Alternate gene names: 222525094

Gene position: 2321252-2319933 (Counterclockwise)

Preceding gene: 222525095

Following gene: 222525093

Centisome position: 44.06

GC content: 63.56

Gene sequence:

>1320_bases
ATGATCGATATCAAAATGCCTCAGCTCGGTGAGAGTGTCACCGAGGGCACAGTTGGGCGCTGGCTCAAGCGGCCCGGCGA
TCCGGTCGCTAAATACGAACCGCTGCTCGAAGTGGTGACAGACAAAGTTGACACCGAAGTGCCGGCTCCTGAAGCCGGCG
TACTCCACGAGATTCTGGTACCGGAGGGGGAGACAGTGCGGGTCGGGACGGTCATCGCGCGCCTGGCCCCGGCAGGCGCA
ACGGTACCTGCAACACCGGCTTCGGCACCAGCCGCAACCACCGTCGCCGCAACGGCATCAGCTACCACCACCGTGACGGC
TACCGTAGCTCCACCGGTAGCAGATGGGCGTAACACCTATCTTTCGCCGGTGGTTGCCCGCTTACTGTCCGAGCACAACC
TCGATCCCGGCCAGATTCGCGGTACCGGTCAGGGTGGCCGTATCACCAAGCAGGACGTGTTGCGCTTCCTGGCCGAACGA
CAACAACAACCGTCTGCGCCGGTGGCGGCGCCGGTCGCTCCCACCCCTGTCGCACCCTCGCCGGCCCCGGCACCAGCCCC
CGCACCGGTAAGTCCAACCCCGGCACCGACCCCGGCACCGACCCCGGCACCAGCCCCTGCACCGGCAACCTTCGATATTC
CTGCGGATGCAGAATTGGTGCCACTCACCCCGATGCGACGCAGTATTGCCGAACATATGGTACGTTCGGTGCGCACCTCA
CCCCACGTGACCACGGTGATGGAGGTCGATCTGAGTCGCGTCATTGCCCATCGCGCCGCCCACCAGGAAGCGTTCAGTCG
CCAGGGTGTGCGTCTGACCATGACCCCCTATTTTGTGATGGCAGCAGTTGCCGGCCTCCAGGCAGTGCCGGTGTTCAACG
GCAGTTTCACCGACCAGGGGATCATCCTGCATCGGCGGATCAACATCGGCGTAGCGGTCGCGCTGCAGGAAGGCTTGCTG
GTGCCGGTAATCCCTGATGCCGACGAGAAGAATCTGCTCGGTCTGGCCCGTGCCGTCAGCGACCTCGCCGAGCGCGCTCG
CACCAAACGATTGCGCCCGGAAGAGACCCAGGGTGGTACCTTCACTATCACCAATCACGGTGTGACCGGCAGCCTCTTCG
CCACCCCGATCATCAACCAGCCCCAGGCCGGCATCCTCGGCGTCGGCGCGATTGTCAAACGCCCGGTCGTGATCACGCAA
AACGGGCTTGATGCAATCGCCCATTCGTCCGCTCTGTTACCTCTCATTCACCTTCGATCACCGGATTGCCGATGGCGCGA
CCGCCGATCAGTTCCTGGCAACGGTGAAAAAACGCCTTGA

Upstream 100 bases:

>100_bases
GCAATGCGGCGGCTGGCCGCTTACTGATGGCTGTCATTAGTCCTGCGACTGATTGGAGAGTGGACACATTCTTTCGTGAG
TTACGTAGCAGGTGAGCTTC

Downstream 100 bases:

>100_bases
AGAGTGGGAAGGATAGGCCCCACACATAACGAATGCGCTTGAACCGCTTCAGAATCGGTTCAAGCGCATTCCTTGCGCCA
TACACGACGCAACCCTCACC

Product: hypothetical protein

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIDIKMPQLGESVTEGTVGRWLKRPGDPVAKYEPLLEVVTDKVDTEVPAPEAGVLHEILVPEGETVRVGTVIARLAPAGA
TVPATPASAPAATTVAATASATTTVTATVAPPVADGRNTYLSPVVARLLSEHNLDPGQIRGTGQGGRITKQDVLRFLAER
QQQPSAPVAAPVAPTPVAPSPAPAPAPAPVSPTPAPTPAPTPAPAPAPATFDIPADAELVPLTPMRRSIAEHMVRSVRTS
PHVTTVMEVDLSRVIAHRAAHQEAFSRQGVRLTMTPYFVMAAVAGLQAVPVFNGSFTDQGIILHRRINIGVAVALQEGLL
VPVIPDADEKNLLGLARAVSDLAERARTKRLRPEETQGGTFTITNHGVTGSLFATPIINQPQAGILGVGAIVKRPVVITQ
NGLDAIAHSSALLPLIHLRSPDCRWRDRRSVPGNGEKTP

Sequences:

>Translated_439_residues
MIDIKMPQLGESVTEGTVGRWLKRPGDPVAKYEPLLEVVTDKVDTEVPAPEAGVLHEILVPEGETVRVGTVIARLAPAGA
TVPATPASAPAATTVAATASATTTVTATVAPPVADGRNTYLSPVVARLLSEHNLDPGQIRGTGQGGRITKQDVLRFLAER
QQQPSAPVAAPVAPTPVAPSPAPAPAPAPVSPTPAPTPAPTPAPAPAPATFDIPADAELVPLTPMRRSIAEHMVRSVRTS
PHVTTVMEVDLSRVIAHRAAHQEAFSRQGVRLTMTPYFVMAAVAGLQAVPVFNGSFTDQGIILHRRINIGVAVALQEGLL
VPVIPDADEKNLLGLARAVSDLAERARTKRLRPEETQGGTFTITNHGVTGSLFATPIINQPQAGILGVGAIVKRPVVITQ
NGLDAIAHSSALLPLIHLRSPDCRWRDRRSVPGNGEKTP
>Mature_439_residues
MIDIKMPQLGESVTEGTVGRWLKRPGDPVAKYEPLLEVVTDKVDTEVPAPEAGVLHEILVPEGETVRVGTVIARLAPAGA
TVPATPASAPAATTVAATASATTTVTATVAPPVADGRNTYLSPVVARLLSEHNLDPGQIRGTGQGGRITKQDVLRFLAER
QQQPSAPVAAPVAPTPVAPSPAPAPAPAPVSPTPAPTPAPTPAPAPAPATFDIPADAELVPLTPMRRSIAEHMVRSVRTS
PHVTTVMEVDLSRVIAHRAAHQEAFSRQGVRLTMTPYFVMAAVAGLQAVPVFNGSFTDQGIILHRRINIGVAVALQEGLL
VPVIPDADEKNLLGLARAVSDLAERARTKRLRPEETQGGTFTITNHGVTGSLFATPIINQPQAGILGVGAIVKRPVVITQ
NGLDAIAHSSALLPLIHLRSPDCRWRDRRSVPGNGEKTP

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI203098753, Length=409, Percent_Identity=32.518337408313, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI203098816, Length=408, Percent_Identity=32.1078431372549, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=28.8329519450801, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI110671329, Length=399, Percent_Identity=26.3157894736842, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI19923748, Length=180, Percent_Identity=41.1111111111111, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI260898739, Length=91, Percent_Identity=48.3516483516484, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786946, Length=435, Percent_Identity=34.2528735632184, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=29.4554455445545, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=402, Percent_Identity=24.6268656716418, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=27.6404494382022, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=300, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=282, Percent_Identity=28.0141843971631, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=255, Percent_Identity=32.5490196078431, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6324258, Length=177, Percent_Identity=29.3785310734463, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18859875, Length=404, Percent_Identity=26.980198019802, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24645909, Length=180, Percent_Identity=37.2222222222222, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI20129315, Length=180, Percent_Identity=32.2222222222222, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24582497, Length=180, Percent_Identity=32.2222222222222, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 45959; Mature: 45959

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIKMPQLGESVTEGTVGRWLKRPGDPVAKYEPLLEVVTDKVDTEVPAPEAGVLHEILV
CCCCCCCHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHC
PEGETVRVGTVIARLAPAGATVPATPASAPAATTVAATASATTTVTATVAPPVADGRNTY
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEECCCCEEEEEEECCCCCCCCCHH
LSPVVARLLSEHNLDPGQIRGTGQGGRITKQDVLRFLAERQQQPSAPVAAPVAPTPVAPS
HHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAPAPAPAPVSPTPAPTPAPTPAPAPAPATFDIPADAELVPLTPMRRSIAEHMVRSVRTS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEECCCHHHHHHHHHHHHHHCCC
PHVTTVMEVDLSRVIAHRAAHQEAFSRQGVRLTMTPYFVMAAVAGLQAVPVFNGSFTDQG
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCC
IILHRRINIGVAVALQEGLLVPVIPDADEKNLLGLARAVSDLAERARTKRLRPEETQGGT
EEEEEEECCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FTITNHGVTGSLFATPIINQPQAGILGVGAIVKRPVVITQNGLDAIAHSSALLPLIHLRS
EEEECCCCCCCEEECCCCCCCCCCEEEHHHHHHCCEEEECCCHHHHHHCCHHEEEEECCC
PDCRWRDRRSVPGNGEKTP
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDIKMPQLGESVTEGTVGRWLKRPGDPVAKYEPLLEVVTDKVDTEVPAPEAGVLHEILV
CCCCCCCHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHC
PEGETVRVGTVIARLAPAGATVPATPASAPAATTVAATASATTTVTATVAPPVADGRNTY
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEECCCCEEEEEEECCCCCCCCCHH
LSPVVARLLSEHNLDPGQIRGTGQGGRITKQDVLRFLAERQQQPSAPVAAPVAPTPVAPS
HHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAPAPAPAPVSPTPAPTPAPTPAPAPAPATFDIPADAELVPLTPMRRSIAEHMVRSVRTS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEECCCHHHHHHHHHHHHHHCCC
PHVTTVMEVDLSRVIAHRAAHQEAFSRQGVRLTMTPYFVMAAVAGLQAVPVFNGSFTDQG
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCC
IILHRRINIGVAVALQEGLLVPVIPDADEKNLLGLARAVSDLAERARTKRLRPEETQGGT
EEEEEEECCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FTITNHGVTGSLFATPIINQPQAGILGVGAIVKRPVVITQNGLDAIAHSSALLPLIHLRS
EEEECCCCCCCEEECCCCCCCCCCEEEHHHHHHCCEEEECCCHHHHHHCCHHEEEEECCC
PDCRWRDRRSVPGNGEKTP
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]