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The map label for this gene is proB [H]

Identifier: 222524933

GI number: 222524933

Start: 2098502

End: 2099608

Strand: Direct

Name: proB [H]

Synonym: Chy400_1667

Alternate gene names: 222524933

Gene position: 2098502-2099608 (Clockwise)

Preceding gene: 222524932

Following gene: 222524934

Centisome position: 39.83

GC content: 57.18

Gene sequence:

>1107_bases
ATGAGCCGATTCGTTGTCAAACTAGGTACAAGCGTCTTAACGGCCGGCACAGATCGCCTCCATCGCCCTTACTTTGTCGA
ACTGGCCCGCCAAATCGTTCGTCTGAAAGATGCCGGTCACGAAGTCGTCCTTGTCTCATCAGGGGCTGTTGCCGCAGGCA
AAGAACGCCTTGGTGTCAATCCTCATCACCGCAGCAATATTCCCCTGAAGCAGGTCTTTGCTGCGGTCGGTCAGAGTCGC
CTGATGCACATTTACGAACAAATCTTTGAGTTGTACGGTTTACAGGTTGCGCAAGCCCTGCTCACCCGTGACGACCTGCG
CGACCGACGGCGTTACCTCAATGCCCGTAATACGCTCACCCTTTGCCTGGAGCAGGGTATCGTGCCAATCATCAATGAAA
ACGATGCTGTCGTCACTGCCGAGATTCGAGTGGGCGACAACGACAACCTCTCAGCACTGGTGGCCGGCTTGATCGACGCT
GACCTGCTCTTAATTTTGACCGACATTGATGGGGTGTACAGCGCCGATCCACGCAGTGATCCAAACGCGGAACTGATCCG
CGAAATACCAGTTATTGACGAACGTGTCTGGGCGATAGCCGGTGGCAGCGGTACCCATCGTGGCACCGGCGGCATGCAGA
CGAAGATTCAGGCTGCCGATCTGGCCACCCGTTCAGGCGTAGCTGTGGTGATTGCAGCCGGCCACGAGCCAGATGTGATT
GTACGGGTGGCGAATGGTGAACGTATCGGAACCTTCTTCCCGGCTACCACAACTCACCCCGATGCGCGACAGCGGTGGAT
TCTGGCCGAAACAGTTCGTCATTCGCGCATTGTGGTGGATGAAGGCGCAACGACCGCGCTGACCCGACACGGCAAGAGTC
TGCTGGCCGCGGGTATCTGCGAGGTGAGTGGTGAGTTTGACCGCGGGCAAACTGTGCGCATCTTTGCCCGCGATGGGCGC
GAGATAGCGCGCGGCCTGACCCAGTACCGATCCAGCGATCTCCGTATGATTGCCGGTCTCCGTTCATCACAGATCGTTTC
CGTTTTAGGGTATGATTATGGGCCGGAGGTCGTCCATCGCGACGATATGGTTGTACTGAGTGGGTAG

Upstream 100 bases:

>100_bases
AGGCAGGAAACGAAAGCCATACAACCTTCAGGCATGCAACGGGGCGAGTATGAACTCGCCCTCATTGTTACAAAGCAGCG
AATGTAGAGCAGTACAGATT

Downstream 100 bases:

>100_bases
AAATCTACGCAGACGGTGTCGTTGAGAGGATATTGGCCAATAAACCAGAAACAAACATTGCGAGCAGACACCACAGCACG
TACAGCACAGAGAGCTAACC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSRFVVKLGTSVLTAGTDRLHRPYFVELARQIVRLKDAGHEVVLVSSGAVAAGKERLGVNPHHRSNIPLKQVFAAVGQSR
LMHIYEQIFELYGLQVAQALLTRDDLRDRRRYLNARNTLTLCLEQGIVPIINENDAVVTAEIRVGDNDNLSALVAGLIDA
DLLLILTDIDGVYSADPRSDPNAELIREIPVIDERVWAIAGGSGTHRGTGGMQTKIQAADLATRSGVAVVIAAGHEPDVI
VRVANGERIGTFFPATTTHPDARQRWILAETVRHSRIVVDEGATTALTRHGKSLLAAGICEVSGEFDRGQTVRIFARDGR
EIARGLTQYRSSDLRMIAGLRSSQIVSVLGYDYGPEVVHRDDMVVLSG

Sequences:

>Translated_368_residues
MSRFVVKLGTSVLTAGTDRLHRPYFVELARQIVRLKDAGHEVVLVSSGAVAAGKERLGVNPHHRSNIPLKQVFAAVGQSR
LMHIYEQIFELYGLQVAQALLTRDDLRDRRRYLNARNTLTLCLEQGIVPIINENDAVVTAEIRVGDNDNLSALVAGLIDA
DLLLILTDIDGVYSADPRSDPNAELIREIPVIDERVWAIAGGSGTHRGTGGMQTKIQAADLATRSGVAVVIAAGHEPDVI
VRVANGERIGTFFPATTTHPDARQRWILAETVRHSRIVVDEGATTALTRHGKSLLAAGICEVSGEFDRGQTVRIFARDGR
EIARGLTQYRSSDLRMIAGLRSSQIVSVLGYDYGPEVVHRDDMVVLSG
>Mature_367_residues
SRFVVKLGTSVLTAGTDRLHRPYFVELARQIVRLKDAGHEVVLVSSGAVAAGKERLGVNPHHRSNIPLKQVFAAVGQSRL
MHIYEQIFELYGLQVAQALLTRDDLRDRRRYLNARNTLTLCLEQGIVPIINENDAVVTAEIRVGDNDNLSALVAGLIDAD
LLLILTDIDGVYSADPRSDPNAELIREIPVIDERVWAIAGGSGTHRGTGGMQTKIQAADLATRSGVAVVIAAGHEPDVIV
RVANGERIGTFFPATTTHPDARQRWILAETVRHSRIVVDEGATTALTRHGKSLLAAGICEVSGEFDRGQTVRIFARDGRE
IARGLTQYRSSDLRMIAGLRSSQIVSVLGYDYGPEVVHRDDMVVLSG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=283, Percent_Identity=37.4558303886926, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI21361368, Length=285, Percent_Identity=37.1929824561403, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1786437, Length=361, Percent_Identity=55.1246537396122, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17569969, Length=269, Percent_Identity=34.9442379182156, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=269, Percent_Identity=34.9442379182156, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=35.1807228915663, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=32.1256038647343, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI21357643, Length=302, Percent_Identity=34.1059602649007, Blast_Score=151, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40103; Mature: 39972

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFVVKLGTSVLTAGTDRLHRPYFVELARQIVRLKDAGHEVVLVSSGAVAAGKERLGVN
CCCEEEECCCHHEECCHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCC
PHHRSNIPLKQVFAAVGQSRLMHIYEQIFELYGLQVAQALLTRDDLRDRRRYLNARNTLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LCLEQGIVPIINENDAVVTAEIRVGDNDNLSALVAGLIDADLLLILTDIDGVYSADPRSD
HHHHCCCEEEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
PNAELIREIPVIDERVWAIAGGSGTHRGTGGMQTKIQAADLATRSGVAVVIAAGHEPDVI
CCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHCCCCEEEEEEECCCCCEE
VRVANGERIGTFFPATTTHPDARQRWILAETVRHSRIVVDEGATTALTRHGKSLLAAGIC
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCHHHHHHHHH
EVSGEFDRGQTVRIFARDGREIARGLTQYRSSDLRMIAGLRSSQIVSVLGYDYGPEVVHR
HCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHEECCCCCHHHHHHHCCCCCCCEEEC
DDMVVLSG
CCEEEEEC
>Mature Secondary Structure 
SRFVVKLGTSVLTAGTDRLHRPYFVELARQIVRLKDAGHEVVLVSSGAVAAGKERLGVN
CCEEEECCCHHEECCHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCC
PHHRSNIPLKQVFAAVGQSRLMHIYEQIFELYGLQVAQALLTRDDLRDRRRYLNARNTLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LCLEQGIVPIINENDAVVTAEIRVGDNDNLSALVAGLIDADLLLILTDIDGVYSADPRSD
HHHHCCCEEEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
PNAELIREIPVIDERVWAIAGGSGTHRGTGGMQTKIQAADLATRSGVAVVIAAGHEPDVI
CCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHCCCCEEEEEEECCCCCEE
VRVANGERIGTFFPATTTHPDARQRWILAETVRHSRIVVDEGATTALTRHGKSLLAAGIC
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCHHHHHHHHH
EVSGEFDRGQTVRIFARDGREIARGLTQYRSSDLRMIAGLRSSQIVSVLGYDYGPEVVHR
HCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHEECCCCCHHHHHHHCCCCCCCEEEC
DDMVVLSG
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA