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The map label for this gene is gltX

Identifier: 222524934

GI number: 222524934

Start: 2099730

End: 2101241

Strand: Direct

Name: gltX

Synonym: Chy400_1668

Alternate gene names: 222524934

Gene position: 2099730-2101241 (Clockwise)

Preceding gene: 222524933

Following gene: 222524936

Centisome position: 39.85

GC content: 58.93

Gene sequence:

>1512_bases
ATGAGTTCCATCGAAGGGCCGGTGCGGGTGCGCTTTGCACCCAGCCCAACCGGTAGTTTGCACATTGGCGGTGTACGCAC
TGCCCTTTTCAACTGGCTCTGTGCCCGCCACTACGGGGGGCAATTTATTCTACGGATCGAAGATACCGACGAAAAGCGAT
TCGTGCCGGGCGCGGCTGACGACATTAGCGCCTCACTGCGCTGGGTGGGTATCGATTGGGACGAAGGGCCAGATGTGGGT
GGCCCTTACGGCCCTTACGTGCAATCAGAACGGTTTGAGCAGGGAATCTATCAGCCATTCATCAACCAGCTTCTGGAAGC
CGGTCTTGCCTACATGTCGTTTACCACCGAAGAAGAACTGGCTCAGATGCGGGCCGCAGCGGAAGCTGCTGGCATCAAGG
CATTTCGCTTCCGTGGCCCGGAGCGTGACTGGCCGCTTGAGCGCCAGCGTGAAGAGGCGGCCAGTGGCAAGCCGTACACC
ATCCGGCTCAAAACGCCCCTTGAAGGCGAAACTCGCTTTCGCGATCTGATCCGCGGTGGTGACGAGATTGTGGTGCAGAA
TAATCAGTTGCAAGACATTGTGTTGATCAAATCGACCGGAATGCCGGTCTACCACTTCGCCCATCTGGTCGATGATCACT
TGATGAAGATCACTCACGTTATGCGTGGCGAAGAGTGGGTACCCAGTACCCCGTACCACGTTCTGCTTTACGACTTCTTC
GGCTGGCCGCGCCCGGTCTTTGCCCATTTGCCGGCGATTCTGCGGCAAGATGGCCGTGGCAAGTTGTCGAAGCGCAAAGA
CGATGTAGCGACACAGCGATTTCGTGAGCGTGGCTACCTGCCGGAGACAATCTTCAACTATCTCGCCTTGCAGGGATGGA
GCTACGATGGCGTGACCGAAATTATGACGCGCGACGAGTTGATTGCGCGTTTCACGCTTGAGCGAATACAACCCTCGCCG
GCGCGCTGGAACCCTGAGAAGCTGCGCGACATGAACGGTATCTACATCCGTAAACTGACGACAGAGCAGTTGGCCGAGCG
CGTGCTGCCGTTCATGCAACGAGCGGGTCTGATCGGCGATCCGGCAAGCGAGACGGAACGCGCGTATCTCATCAGTTTGA
TCCCATTGATTCACGAGCGGCTGGAAGAGTTGCAGGAAGCGCCTGATCTGCTGGCCTTCTTCTACCAGGATGTGGTACCC
GGCGAGACGTACAACCCTGCCGATCTGATTCCAAAGAAGCACGACGCGGCGCAAACCGTCGCCTTACTGCGGGCTGCGCA
CGAAGCTCTGAGTCAGCAAACCGATTGGACACCACCCGCGCTGGAGACAACGCTGCGCGCCCTCTGCGAACAATTGCAGG
TGAAGCCGGGGCCGCTCTTCGGGGCGATTCGCGTCGCCATTACCGGGCGCAGCGTGGCACCACCGCTGTTTGATACGCTC
GCCGGTGTAGGACGCGAACGGAGTCTCTCGCGACTGGCGGCGGCGATTGCCGCACTGGAGAACCTGGCATGA

Upstream 100 bases:

>100_bases
TTGAGAGGATATTGGCCAATAAACCAGAAACAAACATTGCGAGCAGACACCACAGCACGTACAGCACAGAGAGCTAACCT
ATCACAACAAGGAGCATTCC

Downstream 100 bases:

>100_bases
GCACTGTACACTGGGAAACCAGTATTCATCACGACGGATCACCGATGTATCTGCGCTTCAGTGGCAGACCAGGTGACACA
GCGGTATTTCGGCTCCGTAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSSIEGPVRVRFAPSPTGSLHIGGVRTALFNWLCARHYGGQFILRIEDTDEKRFVPGAADDISASLRWVGIDWDEGPDVG
GPYGPYVQSERFEQGIYQPFINQLLEAGLAYMSFTTEEELAQMRAAAEAAGIKAFRFRGPERDWPLERQREEAASGKPYT
IRLKTPLEGETRFRDLIRGGDEIVVQNNQLQDIVLIKSTGMPVYHFAHLVDDHLMKITHVMRGEEWVPSTPYHVLLYDFF
GWPRPVFAHLPAILRQDGRGKLSKRKDDVATQRFRERGYLPETIFNYLALQGWSYDGVTEIMTRDELIARFTLERIQPSP
ARWNPEKLRDMNGIYIRKLTTEQLAERVLPFMQRAGLIGDPASETERAYLISLIPLIHERLEELQEAPDLLAFFYQDVVP
GETYNPADLIPKKHDAAQTVALLRAAHEALSQQTDWTPPALETTLRALCEQLQVKPGPLFGAIRVAITGRSVAPPLFDTL
AGVGRERSLSRLAAAIAALENLA

Sequences:

>Translated_503_residues
MSSIEGPVRVRFAPSPTGSLHIGGVRTALFNWLCARHYGGQFILRIEDTDEKRFVPGAADDISASLRWVGIDWDEGPDVG
GPYGPYVQSERFEQGIYQPFINQLLEAGLAYMSFTTEEELAQMRAAAEAAGIKAFRFRGPERDWPLERQREEAASGKPYT
IRLKTPLEGETRFRDLIRGGDEIVVQNNQLQDIVLIKSTGMPVYHFAHLVDDHLMKITHVMRGEEWVPSTPYHVLLYDFF
GWPRPVFAHLPAILRQDGRGKLSKRKDDVATQRFRERGYLPETIFNYLALQGWSYDGVTEIMTRDELIARFTLERIQPSP
ARWNPEKLRDMNGIYIRKLTTEQLAERVLPFMQRAGLIGDPASETERAYLISLIPLIHERLEELQEAPDLLAFFYQDVVP
GETYNPADLIPKKHDAAQTVALLRAAHEALSQQTDWTPPALETTLRALCEQLQVKPGPLFGAIRVAITGRSVAPPLFDTL
AGVGRERSLSRLAAAIAALENLA
>Mature_502_residues
SSIEGPVRVRFAPSPTGSLHIGGVRTALFNWLCARHYGGQFILRIEDTDEKRFVPGAADDISASLRWVGIDWDEGPDVGG
PYGPYVQSERFEQGIYQPFINQLLEAGLAYMSFTTEEELAQMRAAAEAAGIKAFRFRGPERDWPLERQREEAASGKPYTI
RLKTPLEGETRFRDLIRGGDEIVVQNNQLQDIVLIKSTGMPVYHFAHLVDDHLMKITHVMRGEEWVPSTPYHVLLYDFFG
WPRPVFAHLPAILRQDGRGKLSKRKDDVATQRFRERGYLPETIFNYLALQGWSYDGVTEIMTRDELIARFTLERIQPSPA
RWNPEKLRDMNGIYIRKLTTEQLAERVLPFMQRAGLIGDPASETERAYLISLIPLIHERLEELQEAPDLLAFFYQDVVPG
ETYNPADLIPKKHDAAQTVALLRAAHEALSQQTDWTPPALETTLRALCEQLQVKPGPLFGAIRVAITGRSVAPPLFDTLA
GVGRERSLSRLAAAIAALENLA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=507, Percent_Identity=34.3195266272189, Blast_Score=253, Evalue=4e-67,
Organism=Escherichia coli, GI1788743, Length=497, Percent_Identity=36.4185110663984, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI226510925, Length=258, Percent_Identity=32.5581395348837, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1786895, Length=258, Percent_Identity=24.4186046511628, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=490, Percent_Identity=31.8367346938775, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324540, Length=512, Percent_Identity=32.6171875, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324742, Length=346, Percent_Identity=24.5664739884393, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=506, Percent_Identity=38.3399209486166, Blast_Score=302, Evalue=4e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CHLAA (A9WAX3)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001635143.1
- GeneID:   5828641
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1535
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56742; Mature: 56611

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIEGPVRVRFAPSPTGSLHIGGVRTALFNWLCARHYGGQFILRIEDTDEKRFVPGAAD
CCCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCC
DISASLRWVGIDWDEGPDVGGPYGPYVQSERFEQGIYQPFINQLLEAGLAYMSFTTEEEL
CCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AQMRAAAEAAGIKAFRFRGPERDWPLERQREEAASGKPYTIRLKTPLEGETRFRDLIRGG
HHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCC
DEIVVQNNQLQDIVLIKSTGMPVYHFAHLVDDHLMKITHVMRGEEWVPSTPYHVLLYDFF
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHC
GWPRPVFAHLPAILRQDGRGKLSKRKDDVATQRFRERGYLPETIFNYLALQGWSYDGVTE
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHH
IMTRDELIARFTLERIQPSPARWNPEKLRDMNGIYIRKLTTEQLAERVLPFMQRAGLIGD
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCCCC
PASETERAYLISLIPLIHERLEELQEAPDLLAFFYQDVVPGETYNPADLIPKKHDAAQTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHH
ALLRAAHEALSQQTDWTPPALETTLRALCEQLQVKPGPLFGAIRVAITGRSVAPPLFDTL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHH
AGVGRERSLSRLAAAIAALENLA
HCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSIEGPVRVRFAPSPTGSLHIGGVRTALFNWLCARHYGGQFILRIEDTDEKRFVPGAAD
CCCCCCEEEEECCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCC
DISASLRWVGIDWDEGPDVGGPYGPYVQSERFEQGIYQPFINQLLEAGLAYMSFTTEEEL
CCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AQMRAAAEAAGIKAFRFRGPERDWPLERQREEAASGKPYTIRLKTPLEGETRFRDLIRGG
HHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCC
DEIVVQNNQLQDIVLIKSTGMPVYHFAHLVDDHLMKITHVMRGEEWVPSTPYHVLLYDFF
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHC
GWPRPVFAHLPAILRQDGRGKLSKRKDDVATQRFRERGYLPETIFNYLALQGWSYDGVTE
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHH
IMTRDELIARFTLERIQPSPARWNPEKLRDMNGIYIRKLTTEQLAERVLPFMQRAGLIGD
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCCCC
PASETERAYLISLIPLIHERLEELQEAPDLLAFFYQDVVPGETYNPADLIPKKHDAAQTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHH
ALLRAAHEALSQQTDWTPPALETTLRALCEQLQVKPGPLFGAIRVAITGRSVAPPLFDTL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHH
AGVGRERSLSRLAAAIAALENLA
HCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA