Click here to switch to the map view.

The map label for this gene is tig [H]

Identifier: 222524925

GI number: 222524925

Start: 2088727

End: 2090262

Strand: Direct

Name: tig [H]

Synonym: Chy400_1659

Alternate gene names: 222524925

Gene position: 2088727-2090262 (Clockwise)

Preceding gene: 222524924

Following gene: 222524926

Centisome position: 39.64

GC content: 52.73

Gene sequence:

>1536_bases
ATGGCGCGAAGCGGTATTGTGTTTAAGGATACAACAGTGAAAGTTACTACTGAGAAACTACCAAAGAGCCTGATCGCATT
GCAGATTGAGATCGACCGCGATCAGTTCGAGCGCAGTCTTGATCAGGCGGCACGTCGGCTATCACAAAAGTTTCCGATCC
AGGGCTTCCGTCCCGGTAAGGCACCTCGTTTTATCATTGAACGCACCTTCGGGCGCGAGGCATTGATTGAAGAAGCGACG
GAAGAGCTGATTAACACCGGCTATCGCAAGGCCATCAAGCAAGAAAACATCGAAGTCGTTGGTCCGCCAAATCTTGATAA
GATTCATTCCATCGAACCATTTGTCTTCACCGTTCATGTGCCGGTACCTCCCACGGTTACGCTGCCCGATTACCGTAGCA
TTCATGTACCGCTAGAGGTAGAACCGATCACCGACGAGATGGTCGAAGCGGCGTTAGAACAGATTCGTGAAAAACACCTC
ACGTTGCAGGAACTTGACGAACCACGACCGGCTCAGCAAGGTGATCAGCTACAGGTGCGTTTGAAGACGGAGATCGAGGG
AGAAGAAGAGGAAGCAACTGAAGAGCAGTCTGAAGAAGCTGCTGATGATTCTGAAGCCAAGGCCGAGACCGCAGCCGACA
CCGAACAGACCGATGACGAGGAGGAAGAGGAGGAAGATGACGAAATCGAGAATGAAGGTAGCGAAGAGACGTTACCTCTC
GAACCGAATCGCCTCGTCCCCGAACTGTACGAAGGTTTGATCGGCACGAGCGTCGGTGAGAAGAAAACCATCGTTGCTGT
TTTACCTGAAGATCACCATGAAGAGTCGCTGCGTGGTAAAAAGATAACCTTCCACGTCGAGATACTCGACATCAAACGAC
GGATCGTCCCGGCGTGGGAAGAGCTGCCGGCGCTTGAGAGCTTCAACGGCACATTTGAGGAATTTCGTGCGCATGTGCGC
GAGAACCTCGCCAATCAAGAGCGCAAACGTGCCGAACAAGAGCAGCTTAATGCGTACATCGAGCAGCTCGTTGAGCAGAC
CACGTTTGATATTCCCGATGTGATGATCCGCGATGTTGCGCACTCGATGCTCCATGAGCAAGAGCAGCAGTTCGCACGCT
ATGGAATCACCCTTGACCAGGTCTTGCAATATCGCGGTGTGACCCACGATCAGGCGGTTGAAGAGCTTATCCCTGAAGCC
GAGAAGCAGGTCAAGGTCACACTGGCGCTCAGTGAAGTGATCAAACAGGAGGGAATCACGGTCGGCGAGGATGAGATCGA
GGCCGAGATTCAGACGATTCTGGAAAGCTACGACGAGAAGCAACGGCCACAGGTCGAGAGAACCCTGCGCGGTCAATTAC
GCTCGTCGGTAGCAAATGTAGTCCTTGATAAAAAGCTACGTGCCCGTCTCAGTGCAATAGCGAGCGGCAATTCAACAGAC
ACCTCTGATTCCAGCCCTGCCGCTCCCGACGCAGAAGGTGCAACTGATGTTACAAATTCCGATGAACCGGTGAGCCGGTC
ATCGAACGAAGCATGA

Upstream 100 bases:

>100_bases
GCCGCGTAATCTCTTCGCATAGACCAGATAGACGTGCTATAATGCACCATACAGCGCACCGATGTGTGCGTATTTTCGAG
CAAGCAATAAGCCACTTCTG

Downstream 100 bases:

>100_bases
GGAGTGTCGTATGAATTGGTCGTCTCGTTACAGTCACGATTGGCGTAGTATGCCGGGACCAGAATGGCTCTCGCAACGAC
CGGAACTACTCATCCCGATG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MARSGIVFKDTTVKVTTEKLPKSLIALQIEIDRDQFERSLDQAARRLSQKFPIQGFRPGKAPRFIIERTFGREALIEEAT
EELINTGYRKAIKQENIEVVGPPNLDKIHSIEPFVFTVHVPVPPTVTLPDYRSIHVPLEVEPITDEMVEAALEQIREKHL
TLQELDEPRPAQQGDQLQVRLKTEIEGEEEEATEEQSEEAADDSEAKAETAADTEQTDDEEEEEEDDEIENEGSEETLPL
EPNRLVPELYEGLIGTSVGEKKTIVAVLPEDHHEESLRGKKITFHVEILDIKRRIVPAWEELPALESFNGTFEEFRAHVR
ENLANQERKRAEQEQLNAYIEQLVEQTTFDIPDVMIRDVAHSMLHEQEQQFARYGITLDQVLQYRGVTHDQAVEELIPEA
EKQVKVTLALSEVIKQEGITVGEDEIEAEIQTILESYDEKQRPQVERTLRGQLRSSVANVVLDKKLRARLSAIASGNSTD
TSDSSPAAPDAEGATDVTNSDEPVSRSSNEA

Sequences:

>Translated_511_residues
MARSGIVFKDTTVKVTTEKLPKSLIALQIEIDRDQFERSLDQAARRLSQKFPIQGFRPGKAPRFIIERTFGREALIEEAT
EELINTGYRKAIKQENIEVVGPPNLDKIHSIEPFVFTVHVPVPPTVTLPDYRSIHVPLEVEPITDEMVEAALEQIREKHL
TLQELDEPRPAQQGDQLQVRLKTEIEGEEEEATEEQSEEAADDSEAKAETAADTEQTDDEEEEEEDDEIENEGSEETLPL
EPNRLVPELYEGLIGTSVGEKKTIVAVLPEDHHEESLRGKKITFHVEILDIKRRIVPAWEELPALESFNGTFEEFRAHVR
ENLANQERKRAEQEQLNAYIEQLVEQTTFDIPDVMIRDVAHSMLHEQEQQFARYGITLDQVLQYRGVTHDQAVEELIPEA
EKQVKVTLALSEVIKQEGITVGEDEIEAEIQTILESYDEKQRPQVERTLRGQLRSSVANVVLDKKLRARLSAIASGNSTD
TSDSSPAAPDAEGATDVTNSDEPVSRSSNEA
>Mature_510_residues
ARSGIVFKDTTVKVTTEKLPKSLIALQIEIDRDQFERSLDQAARRLSQKFPIQGFRPGKAPRFIIERTFGREALIEEATE
ELINTGYRKAIKQENIEVVGPPNLDKIHSIEPFVFTVHVPVPPTVTLPDYRSIHVPLEVEPITDEMVEAALEQIREKHLT
LQELDEPRPAQQGDQLQVRLKTEIEGEEEEATEEQSEEAADDSEAKAETAADTEQTDDEEEEEEDDEIENEGSEETLPLE
PNRLVPELYEGLIGTSVGEKKTIVAVLPEDHHEESLRGKKITFHVEILDIKRRIVPAWEELPALESFNGTFEEFRAHVRE
NLANQERKRAEQEQLNAYIEQLVEQTTFDIPDVMIRDVAHSMLHEQEQQFARYGITLDQVLQYRGVTHDQAVEELIPEAE
KQVKVTLALSEVIKQEGITVGEDEIEAEIQTILESYDEKQRPQVERTLRGQLRSSVANVVLDKKLRARLSAIASGNSTDT
SDSSPAAPDAEGATDVTNSDEPVSRSSNEA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=432, Percent_Identity=24.7685185185185, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 57717; Mature: 57585

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSGIVFKDTTVKVTTEKLPKSLIALQIEIDRDQFERSLDQAARRLSQKFPIQGFRPGK
CCCCCEEEECCEEEEEHHHHCHHHEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCC
APRFIIERTFGREALIEEATEELINTGYRKAIKQENIEVVGPPNLDKIHSIEPFVFTVHV
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCEEEEEEE
PVPPTVTLPDYRSIHVPLEVEPITDEMVEAALEQIREKHLTLQELDEPRPAQQGDQLQVR
CCCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEE
LKTEIEGEEEEATEEQSEEAADDSEAKAETAADTEQTDDEEEEEEDDEIENEGSEETLPL
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
EPNRLVPELYEGLIGTSVGEKKTIVAVLPEDHHEESLRGKKITFHVEILDIKRRIVPAWE
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCEEEEEEEEEEHHHHHCCCHH
ELPALESFNGTFEEFRAHVRENLANQERKRAEQEQLNAYIEQLVEQTTFDIPDVMIRDVA
HCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HSMLHEQEQQFARYGITLDQVLQYRGVTHDQAVEELIPEAEKQVKVTLALSEVIKQEGIT
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCHHHHHEEHHHHHHHHHHHCCCC
VGEDEIEAEIQTILESYDEKQRPQVERTLRGQLRSSVANVVLDKKLRARLSAIASGNSTD
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSDSSPAAPDAEGATDVTNSDEPVSRSSNEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARSGIVFKDTTVKVTTEKLPKSLIALQIEIDRDQFERSLDQAARRLSQKFPIQGFRPGK
CCCCEEEECCEEEEEHHHHCHHHEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCC
APRFIIERTFGREALIEEATEELINTGYRKAIKQENIEVVGPPNLDKIHSIEPFVFTVHV
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCEEEEEEE
PVPPTVTLPDYRSIHVPLEVEPITDEMVEAALEQIREKHLTLQELDEPRPAQQGDQLQVR
CCCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEE
LKTEIEGEEEEATEEQSEEAADDSEAKAETAADTEQTDDEEEEEEDDEIENEGSEETLPL
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
EPNRLVPELYEGLIGTSVGEKKTIVAVLPEDHHEESLRGKKITFHVEILDIKRRIVPAWE
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCEEEEEEEEEEHHHHHCCCHH
ELPALESFNGTFEEFRAHVRENLANQERKRAEQEQLNAYIEQLVEQTTFDIPDVMIRDVA
HCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
HSMLHEQEQQFARYGITLDQVLQYRGVTHDQAVEELIPEAEKQVKVTLALSEVIKQEGIT
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHCHHHHHEEHHHHHHHHHHHCCCC
VGEDEIEAEIQTILESYDEKQRPQVERTLRGQLRSSVANVVLDKKLRARLSAIASGNSTD
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TSDSSPAAPDAEGATDVTNSDEPVSRSSNEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA