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The map label for this gene is wcaJ [H]

Identifier: 222524902

GI number: 222524902

Start: 2063570

End: 2064940

Strand: Reverse

Name: wcaJ [H]

Synonym: Chy400_1636

Alternate gene names: 222524902

Gene position: 2064940-2063570 (Counterclockwise)

Preceding gene: 222524903

Following gene: 222524901

Centisome position: 39.19

GC content: 56.09

Gene sequence:

>1371_bases
ATGCTGGAACGCCTGCGCACGCGACTGATCGGGAGCGTTCTCGCGTGGGATCTGATAGCAACATTGCTGTGTCTCGCCCT
CGTCGTTCGCAATGATCCAACTCTTACACGCCAGTGGCCGCTCTACGGCGGTGTTGCTGTGATCTGGACACTGGTATTCA
TCGTTCTGGCCCCACAACGGGCACTGTTCACCCGCACCCTTCTCGAAGCCTTTACCCGTCTCTTCCTCGCTGTACTGCTG
GCGGCAACCTCATTCGCCGGGTTACTCTATCTGGTGAACGGGGTGGCTATTGATCGGGGAACATTCACCTGGTTCGTCAT
TCTTAATCTGGTCGTCCTGGGCGTGATCCATCTGGGATTTCGGACGTTCGCCCGCTGGCGGCAGCAGCGTGGAAATGGGC
GACGAGTACTGGTGGTTGGCGAGGTGAATGCTGCCATCCGCCTGGCCGATGAATTTGTGCGCCGGCCATGGACCGGCGTA
CAAGTGGTGGGGTATGCCTCTGATGAGCGCGATGCCCCAACAAGCCTTCCCCGCCTGGGCGGTTTACACGATCTCGTGAC
CCTGGTGCGTCGGTGTCAGATCGATGAAGTGATCTTCGCGTTGCCACCATCACGCTACGATCAGGTGGTTGAGTTGTCGT
TGCAATTACTCAAAGAGCCGGTGATGATTCACACAGTCCCAACGGCGCTCGATCTGGCGTTTGCCCGGACTCCAGTGGAT
AGTGTAGGTGGTATTCCACTGGTGTCACTCCGCGAGTCGGCACTGACCGACTCGCAACGGGTGGTGAAGCGTCTATTTGA
TATTGTTGTGAGTTTGCTGTTGATCGTGCTGCTCTCTCCTTTAATGCTGCTGATCGCGCTGCTTATCAAACTCGAGTCGC
CGGGGCCGATTATTTTTCGTCAGGAGCGCATTGGTGAGCATGGGCGACGCTTTCAGATGCTGAAGTTTCGCAGCATGTAC
GTCGATGCAGATCGGCGCTGGCGTGAGGTAGCCCAGCGTGATGAACACGGTCGGCTTATTCATAAGATGAAAGATGATCC
GCGCATCACTCGTATTGGGCGAAAATTACGCCGCACATCCCTCGATGAATTACCACAACTGTTCAATGTATTACGCGGCG
AAATGAGTCTGGTGGGGCCGCGACCAGAGATGCCTTATATCGCTGCCGAGTATGAGCCGTGGCAGTGGCAGCGCTTCCGG
GTTCCGCCGGGCATGACCGGCTGGTGGCAGGTGAATGGTCGTAGTGATAAGCCGATGCATTTGCACACCGAAGATGATCT
GTACTACATCCAGAATTATTCATTCTGGCTCGATCTGCGCATCCTGCTCAAAACGGTGATCGTCGTCTGGCAGGGGCATG
GCGCATATTGA

Upstream 100 bases:

>100_bases
TACTATCAACCGGCATACCCCCTGGATGGGTTTGTACCGGTTGACATGGTGTGAAGGCGCGGCCACGACGCCGGCAGGCG
CCTTCACGTAAGGAGCCGGC

Downstream 100 bases:

>100_bases
TAAGATCACGTACAATAGCAGCACAGGCGTAGTTTGCATCCCACGGCATCATCACCAGTAGATGCACAATGTACCCATTG
ACAGACGGCGTGAAGGTGTC

Product: exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLERLRTRLIGSVLAWDLIATLLCLALVVRNDPTLTRQWPLYGGVAVIWTLVFIVLAPQRALFTRTLLEAFTRLFLAVLL
AATSFAGLLYLVNGVAIDRGTFTWFVILNLVVLGVIHLGFRTFARWRQQRGNGRRVLVVGEVNAAIRLADEFVRRPWTGV
QVVGYASDERDAPTSLPRLGGLHDLVTLVRRCQIDEVIFALPPSRYDQVVELSLQLLKEPVMIHTVPTALDLAFARTPVD
SVGGIPLVSLRESALTDSQRVVKRLFDIVVSLLLIVLLSPLMLLIALLIKLESPGPIIFRQERIGEHGRRFQMLKFRSMY
VDADRRWREVAQRDEHGRLIHKMKDDPRITRIGRKLRRTSLDELPQLFNVLRGEMSLVGPRPEMPYIAAEYEPWQWQRFR
VPPGMTGWWQVNGRSDKPMHLHTEDDLYYIQNYSFWLDLRILLKTVIVVWQGHGAY

Sequences:

>Translated_456_residues
MLERLRTRLIGSVLAWDLIATLLCLALVVRNDPTLTRQWPLYGGVAVIWTLVFIVLAPQRALFTRTLLEAFTRLFLAVLL
AATSFAGLLYLVNGVAIDRGTFTWFVILNLVVLGVIHLGFRTFARWRQQRGNGRRVLVVGEVNAAIRLADEFVRRPWTGV
QVVGYASDERDAPTSLPRLGGLHDLVTLVRRCQIDEVIFALPPSRYDQVVELSLQLLKEPVMIHTVPTALDLAFARTPVD
SVGGIPLVSLRESALTDSQRVVKRLFDIVVSLLLIVLLSPLMLLIALLIKLESPGPIIFRQERIGEHGRRFQMLKFRSMY
VDADRRWREVAQRDEHGRLIHKMKDDPRITRIGRKLRRTSLDELPQLFNVLRGEMSLVGPRPEMPYIAAEYEPWQWQRFR
VPPGMTGWWQVNGRSDKPMHLHTEDDLYYIQNYSFWLDLRILLKTVIVVWQGHGAY
>Mature_456_residues
MLERLRTRLIGSVLAWDLIATLLCLALVVRNDPTLTRQWPLYGGVAVIWTLVFIVLAPQRALFTRTLLEAFTRLFLAVLL
AATSFAGLLYLVNGVAIDRGTFTWFVILNLVVLGVIHLGFRTFARWRQQRGNGRRVLVVGEVNAAIRLADEFVRRPWTGV
QVVGYASDERDAPTSLPRLGGLHDLVTLVRRCQIDEVIFALPPSRYDQVVELSLQLLKEPVMIHTVPTALDLAFARTPVD
SVGGIPLVSLRESALTDSQRVVKRLFDIVVSLLLIVLLSPLMLLIALLIKLESPGPIIFRQERIGEHGRRFQMLKFRSMY
VDADRRWREVAQRDEHGRLIHKMKDDPRITRIGRKLRRTSLDELPQLFNVLRGEMSLVGPRPEMPYIAAEYEPWQWQRFR
VPPGMTGWWQVNGRSDKPMHLHTEDDLYYIQNYSFWLDLRILLKTVIVVWQGHGAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=380, Percent_Identity=34.2105263157895, Blast_Score=201, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52346; Mature: 52346

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERLRTRLIGSVLAWDLIATLLCLALVVRNDPTLTRQWPLYGGVAVIWTLVFIVLAPQR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
ALFTRTLLEAFTRLFLAVLLAATSFAGLLYLVNGVAIDRGTFTWFVILNLVVLGVIHLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHH
RTFARWRQQRGNGRRVLVVGEVNAAIRLADEFVRRPWTGVQVVGYASDERDAPTSLPRLG
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCC
GLHDLVTLVRRCQIDEVIFALPPSRYDQVVELSLQLLKEPVMIHTVPTALDLAFARTPVD
CHHHHHHHHHHHCHHHHHEECCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCH
SVGGIPLVSLRESALTDSQRVVKRLFDIVVSLLLIVLLSPLMLLIALLIKLESPGPIIFR
HCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
QERIGEHGRRFQMLKFRSMYVDADRRWREVAQRDEHGRLIHKMKDDPRITRIGRKLRRTS
HHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHCC
LDELPQLFNVLRGEMSLVGPRPEMPYIAAEYEPWQWQRFRVPPGMTGWWQVNGRSDKPMH
HHHHHHHHHHHHCHHHCCCCCCCCCEEEECCCCCCCEEEECCCCCCCEEEECCCCCCCEE
LHTEDDLYYIQNYSFWLDLRILLKTVIVVWQGHGAY
EECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLERLRTRLIGSVLAWDLIATLLCLALVVRNDPTLTRQWPLYGGVAVIWTLVFIVLAPQR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
ALFTRTLLEAFTRLFLAVLLAATSFAGLLYLVNGVAIDRGTFTWFVILNLVVLGVIHLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHH
RTFARWRQQRGNGRRVLVVGEVNAAIRLADEFVRRPWTGVQVVGYASDERDAPTSLPRLG
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCC
GLHDLVTLVRRCQIDEVIFALPPSRYDQVVELSLQLLKEPVMIHTVPTALDLAFARTPVD
CHHHHHHHHHHHCHHHHHEECCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCH
SVGGIPLVSLRESALTDSQRVVKRLFDIVVSLLLIVLLSPLMLLIALLIKLESPGPIIFR
HCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
QERIGEHGRRFQMLKFRSMYVDADRRWREVAQRDEHGRLIHKMKDDPRITRIGRKLRRTS
HHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHCC
LDELPQLFNVLRGEMSLVGPRPEMPYIAAEYEPWQWQRFRVPPGMTGWWQVNGRSDKPMH
HHHHHHHHHHHHCHHHCCCCCCCCCEEEECCCCCCCEEEECCCCCCCEEEECCCCCCCEE
LHTEDDLYYIQNYSFWLDLRILLKTVIVVWQGHGAY
EECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]