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The map label for this gene is kinE [H]

Identifier: 222524838

GI number: 222524838

Start: 1989680

End: 1992070

Strand: Direct

Name: kinE [H]

Synonym: Chy400_1565

Alternate gene names: 222524838

Gene position: 1989680-1992070 (Clockwise)

Preceding gene: 222524837

Following gene: 222524841

Centisome position: 37.76

GC content: 56.29

Gene sequence:

>2391_bases
ATGCACACCATTCCGTCATCCTTGCGTGGTACGCTGATCGGATTGTGGAGTGCTGCTGCGCTGGTCGGAGTCATTTCGAG
TTTGCTGGCCCCGCCGGCAGCATTTGATCTGGCTGCCTGGCTGGCCACCATTCTGTTTGCCCTTGGACTTACCATCTTTG
AGGCGTTGACGATTGTGCGCCAGCATGACCGCCCACTGGCCGTGACTCTGGTCTTGTTGATCAGTGCCATGATCGTTTTG
CCATGGCCCCTCTTTTTGCTGGCAACTGCGATAGGGGCAGTCGCCGGCACCCTGGCGCGTCAGTCCCCATGGTGGCAGGC
GCTGGCGCTGGCCTCTATTCGCTGGATCGCTCTTACCGCCGGCAGCGTGGTGCTTATGCTATTTGTGCAGCAGCATCCCT
TTGGTGCTGATATTGCCTCACCTTTTGCTTTGATCGGCCTGATTGGCTGCGGCTTTGTGATCTATTCGATTGAACGGGTG
GCCAGTGCCAGCCTGTCTTCTCCGGTCACTGATGAGCGTTTTCTGCTGGCATTGCGGTGGCGTATCGATGACGCTGGCTG
GTATGCCCTGATCTTTGCTCCGCTGGGGGGGATGCTGGCTATTCTCTGGCAACAGGAGGTGTGGGCTTTTGCGTTGGGGA
TGGCACCGCTGGCGATGGTTCAAATCAGCCTGCGTCGTCAGTACAGGTTAGAAGAGGCTTTGCGCGTTGCTGATACGCAA
TTGCAAACGGCTAATGCCCGTCTCCGCACCTTGTCGCGTCAGACCGATCATTTGCATAGCCTGATTGTCGCACTCATGAC
CAGCCGTGATGTGCCGACAATGCTCAATCTGTTGGGTGAACGGTTGAGTGCTCTGATGGAAGCCGATTGCGCCTGGGTTA
CCCTTTACGATGACCAGGGTAACCTTCAGTTAGTCGCTTCCCACCATTTACCCGTCTCTACCGAAGGGGTCGGACCGCAA
CCGGTACCGCTCCCGCAAGCCTATGAAACAGCCCTGGAACAGAAGCGGGTGACACTCTTCACCAATCAGCAAGTTCAGAT
GCTGGCGCCGGTGGCTGCCTTGACCGAACGTTCAACATGGCAGGCAGTGATTATGCTTCCGCTGCTCGGCGAGCAAGCCC
CACTTGGTGCCGTTTGTCTGGCATTTGAGACGGTACGGGGGCTGAAAGAGGACGAACAACGTCTACTGGCAGCGTTTGCC
CGTCACGCTACTGCTGTAATCCAGAATGCCCGCCTCTTTCGTCGCTGGCAGGAATCGCAAACCGAGCTGGGGCGTTCGGC
CAAACTGGCTGCGGTGGGTACATTTGCCGCCAGTATTGCCCACGAATTCAACAACCTGCTTTCGGGAATGCTGGGGTATG
CACAACTCGGCCTGGCCGACGATGATGTCGAAATGAAGAACGAGGCTTTGAAGGTCGTGCTCGACACCTGTAAGCGTGGT
CGCAGCATTACCGGCAGCCTGCTCACCTTTGCCCGCCGCCGCGACTCGCGTCGGGAATTGTCTGATCTGCGCGAAGTTGT
TGATGGAACGCTCACTCTTATGGAGATAGAACTCCGTAAGCATAATATTCGCGTTGTCCGGCAGATCGATATGGTACCAC
CGACTATCTGTGATTCTGGTCAGATTTCTCAAGTCTTTTTGAATCTGCTCACCAATGCACGTGATGCGATGAAACCGCAG
GGGGGGACGCTAACCGTATCACTGACCTCGGATAACGAATGGATTACCTTGCAGGTTACTGATACCGGTTGCGGCATCCC
TGAAGCGGTACGTCGCCAGATTTTCCAGCCGTTTGTGACCACCAAGAATACCACCGGTAGTCAATCGGGTACCGGTCTGG
GTCTGGCCGTTTCGTATGGCATCATCAAAAACCACAACGGCCACTTTGATGTCGAGAGCGAGGTCGGGAAGGGGACGACG
ATGACGATCCGTCTACCTATTGTGCAAGAAGAGGAAAGTGAAACGAGCAAGGCCCCGATCCCCTCGCTGCGCATGCTGGT
GATTGATGATGATGAAGCTGCTGCCCGCACACTTACCGCCCTTTTGGAACAGGCGGGTCATCAGGTGACCTATTGCGCAC
ACAGTGCAGAGGCCGTAGCCATCTATACCTCCAATCCGTTCGATATTGTTTTGACCGATGTCGTGATGCCCGAACTCGAT
GGGATCAGCCTCCTCAATGTGTTACGCACCTACGATCCTACGGCGAGTATCATCTTGTTCACCGGCCAGATCGAACCCGA
TCAGGTTGCGGCAATGGCGGCCAGCGGTGCGTATGCCGTGTTGCGTAAACCGTTCTCTGCCGAGGAGCTGATGACGACGG
TTCAGGCAGCCTATCACGACCGCGAACGGGCCAAACGCGCATTGGCAGGCTTCGTCGAAACGGCGCGTTGA

Upstream 100 bases:

>100_bases
CCATTAACTGAACAACATATACAACGAGGAGTTGCCCCATCAACGTTCCGTTTTCCATCCTTGCCCGGGCAATTTCGCCA
TCTGTTGAGGAGAGAATTTC

Downstream 100 bases:

>100_bases
GATAAGGTGCGTGGTTGTCTTCTTTTCACTTCTGGTGCGGCAGAAATGATGACACGCCATCACTCTGGTACCGCATTTGA
TCGCGGATGCGCTGAACCAA

Product: multi-sensor hybrid histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MHTIPSSLRGTLIGLWSAAALVGVISSLLAPPAAFDLAAWLATILFALGLTIFEALTIVRQHDRPLAVTLVLLISAMIVL
PWPLFLLATAIGAVAGTLARQSPWWQALALASIRWIALTAGSVVLMLFVQQHPFGADIASPFALIGLIGCGFVIYSIERV
ASASLSSPVTDERFLLALRWRIDDAGWYALIFAPLGGMLAILWQQEVWAFALGMAPLAMVQISLRRQYRLEEALRVADTQ
LQTANARLRTLSRQTDHLHSLIVALMTSRDVPTMLNLLGERLSALMEADCAWVTLYDDQGNLQLVASHHLPVSTEGVGPQ
PVPLPQAYETALEQKRVTLFTNQQVQMLAPVAALTERSTWQAVIMLPLLGEQAPLGAVCLAFETVRGLKEDEQRLLAAFA
RHATAVIQNARLFRRWQESQTELGRSAKLAAVGTFAASIAHEFNNLLSGMLGYAQLGLADDDVEMKNEALKVVLDTCKRG
RSITGSLLTFARRRDSRRELSDLREVVDGTLTLMEIELRKHNIRVVRQIDMVPPTICDSGQISQVFLNLLTNARDAMKPQ
GGTLTVSLTSDNEWITLQVTDTGCGIPEAVRRQIFQPFVTTKNTTGSQSGTGLGLAVSYGIIKNHNGHFDVESEVGKGTT
MTIRLPIVQEEESETSKAPIPSLRMLVIDDDEAAARTLTALLEQAGHQVTYCAHSAEAVAIYTSNPFDIVLTDVVMPELD
GISLLNVLRTYDPTASIILFTGQIEPDQVAAMAASGAYAVLRKPFSAEELMTTVQAAYHDRERAKRALAGFVETAR

Sequences:

>Translated_796_residues
MHTIPSSLRGTLIGLWSAAALVGVISSLLAPPAAFDLAAWLATILFALGLTIFEALTIVRQHDRPLAVTLVLLISAMIVL
PWPLFLLATAIGAVAGTLARQSPWWQALALASIRWIALTAGSVVLMLFVQQHPFGADIASPFALIGLIGCGFVIYSIERV
ASASLSSPVTDERFLLALRWRIDDAGWYALIFAPLGGMLAILWQQEVWAFALGMAPLAMVQISLRRQYRLEEALRVADTQ
LQTANARLRTLSRQTDHLHSLIVALMTSRDVPTMLNLLGERLSALMEADCAWVTLYDDQGNLQLVASHHLPVSTEGVGPQ
PVPLPQAYETALEQKRVTLFTNQQVQMLAPVAALTERSTWQAVIMLPLLGEQAPLGAVCLAFETVRGLKEDEQRLLAAFA
RHATAVIQNARLFRRWQESQTELGRSAKLAAVGTFAASIAHEFNNLLSGMLGYAQLGLADDDVEMKNEALKVVLDTCKRG
RSITGSLLTFARRRDSRRELSDLREVVDGTLTLMEIELRKHNIRVVRQIDMVPPTICDSGQISQVFLNLLTNARDAMKPQ
GGTLTVSLTSDNEWITLQVTDTGCGIPEAVRRQIFQPFVTTKNTTGSQSGTGLGLAVSYGIIKNHNGHFDVESEVGKGTT
MTIRLPIVQEEESETSKAPIPSLRMLVIDDDEAAARTLTALLEQAGHQVTYCAHSAEAVAIYTSNPFDIVLTDVVMPELD
GISLLNVLRTYDPTASIILFTGQIEPDQVAAMAASGAYAVLRKPFSAEELMTTVQAAYHDRERAKRALAGFVETAR
>Mature_796_residues
MHTIPSSLRGTLIGLWSAAALVGVISSLLAPPAAFDLAAWLATILFALGLTIFEALTIVRQHDRPLAVTLVLLISAMIVL
PWPLFLLATAIGAVAGTLARQSPWWQALALASIRWIALTAGSVVLMLFVQQHPFGADIASPFALIGLIGCGFVIYSIERV
ASASLSSPVTDERFLLALRWRIDDAGWYALIFAPLGGMLAILWQQEVWAFALGMAPLAMVQISLRRQYRLEEALRVADTQ
LQTANARLRTLSRQTDHLHSLIVALMTSRDVPTMLNLLGERLSALMEADCAWVTLYDDQGNLQLVASHHLPVSTEGVGPQ
PVPLPQAYETALEQKRVTLFTNQQVQMLAPVAALTERSTWQAVIMLPLLGEQAPLGAVCLAFETVRGLKEDEQRLLAAFA
RHATAVIQNARLFRRWQESQTELGRSAKLAAVGTFAASIAHEFNNLLSGMLGYAQLGLADDDVEMKNEALKVVLDTCKRG
RSITGSLLTFARRRDSRRELSDLREVVDGTLTLMEIELRKHNIRVVRQIDMVPPTICDSGQISQVFLNLLTNARDAMKPQ
GGTLTVSLTSDNEWITLQVTDTGCGIPEAVRRQIFQPFVTTKNTTGSQSGTGLGLAVSYGIIKNHNGHFDVESEVGKGTT
MTIRLPIVQEEESETSKAPIPSLRMLVIDDDEAAARTLTALLEQAGHQVTYCAHSAEAVAIYTSNPFDIVLTDVVMPELD
GISLLNVLRTYDPTASIILFTGQIEPDQVAAMAASGAYAVLRKPFSAEELMTTVQAAYHDRERAKRALAGFVETAR

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081816, Length=395, Percent_Identity=28.6075949367089, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=32.7586206896552, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI48994928, Length=383, Percent_Identity=27.4151436031332, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=31.404958677686, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1790300, Length=243, Percent_Identity=31.6872427983539, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1788713, Length=245, Percent_Identity=29.3877551020408, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=26.9387755102041, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=32.1739130434783, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789149, Length=218, Percent_Identity=23.8532110091743, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=25.7777777777778, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786837, Length=291, Percent_Identity=24.7422680412371, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1790861, Length=220, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 86792; Mature: 86792

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTIPSSLRGTLIGLWSAAALVGVISSLLAPPAAFDLAAWLATILFALGLTIFEALTIVR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHDRPLAVTLVLLISAMIVLPWPLFLLATAIGAVAGTLARQSPWWQALALASIRWIALTA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GSVVLMLFVQQHPFGADIASPFALIGLIGCGFVIYSIERVASASLSSPVTDERFLLALRW
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
RIDDAGWYALIFAPLGGMLAILWQQEVWAFALGMAPLAMVQISLRRQYRLEEALRVADTQ
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTANARLRTLSRQTDHLHSLIVALMTSRDVPTMLNLLGERLSALMEADCAWVTLYDDQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
NLQLVASHHLPVSTEGVGPQPVPLPQAYETALEQKRVTLFTNQQVQMLAPVAALTERSTW
CEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCC
QAVIMLPLLGEQAPLGAVCLAFETVRGLKEDEQRLLAAFARHATAVIQNARLFRRWQESQ
EEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELGRSAKLAAVGTFAASIAHEFNNLLSGMLGYAQLGLADDDVEMKNEALKVVLDTCKRG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
RSITGSLLTFARRRDSRRELSDLREVVDGTLTLMEIELRKHNIRVVRQIDMVPPTICDSG
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEHHCCCEEEEEECCCCCCCCCCC
QISQVFLNLLTNARDAMKPQGGTLTVSLTSDNEWITLQVTDTGCGIPEAVRRQIFQPFVT
CHHHHHHHHHHCHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHC
TKNTTGSQSGTGLGLAVSYGIIKNHNGHFDVESEVGKGTTMTIRLPIVQEEESETSKAPI
CCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
PSLRMLVIDDDEAAARTLTALLEQAGHQVTYCAHSAEAVAIYTSNPFDIVLTDVVMPELD
CCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCEEEEHHHHHCCCC
GISLLNVLRTYDPTASIILFTGQIEPDQVAAMAASGAYAVLRKPFSAEELMTTVQAAYHD
CHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHH
RERAKRALAGFVETAR
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHTIPSSLRGTLIGLWSAAALVGVISSLLAPPAAFDLAAWLATILFALGLTIFEALTIVR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHDRPLAVTLVLLISAMIVLPWPLFLLATAIGAVAGTLARQSPWWQALALASIRWIALTA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GSVVLMLFVQQHPFGADIASPFALIGLIGCGFVIYSIERVASASLSSPVTDERFLLALRW
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
RIDDAGWYALIFAPLGGMLAILWQQEVWAFALGMAPLAMVQISLRRQYRLEEALRVADTQ
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTANARLRTLSRQTDHLHSLIVALMTSRDVPTMLNLLGERLSALMEADCAWVTLYDDQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
NLQLVASHHLPVSTEGVGPQPVPLPQAYETALEQKRVTLFTNQQVQMLAPVAALTERSTW
CEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCC
QAVIMLPLLGEQAPLGAVCLAFETVRGLKEDEQRLLAAFARHATAVIQNARLFRRWQESQ
EEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELGRSAKLAAVGTFAASIAHEFNNLLSGMLGYAQLGLADDDVEMKNEALKVVLDTCKRG
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
RSITGSLLTFARRRDSRRELSDLREVVDGTLTLMEIELRKHNIRVVRQIDMVPPTICDSG
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEHHCCCEEEEEECCCCCCCCCCC
QISQVFLNLLTNARDAMKPQGGTLTVSLTSDNEWITLQVTDTGCGIPEAVRRQIFQPFVT
CHHHHHHHHHHCHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHC
TKNTTGSQSGTGLGLAVSYGIIKNHNGHFDVESEVGKGTTMTIRLPIVQEEESETSKAPI
CCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
PSLRMLVIDDDEAAARTLTALLEQAGHQVTYCAHSAEAVAIYTSNPFDIVLTDVVMPELD
CCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCEEEEHHHHHCCCC
GISLLNVLRTYDPTASIILFTGQIEPDQVAAMAASGAYAVLRKPFSAEELMTTVQAAYHD
CHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHH
RERAKRALAGFVETAR
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]