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The map label for this gene is trpB2 [H]

Identifier: 222524805

GI number: 222524805

Start: 1952795

End: 1954156

Strand: Reverse

Name: trpB2 [H]

Synonym: Chy400_1532

Alternate gene names: 222524805

Gene position: 1954156-1952795 (Counterclockwise)

Preceding gene: 222524809

Following gene: 222524803

Centisome position: 37.09

GC content: 58.81

Gene sequence:

>1362_bases
GTGGAAACGGTCAAGTATCTACTCAGTGAAGATCGCCTGCCGGAGACCTGGTACAACATCGCTGCCGATTTGCCATCGCT
GCCGCCGCCGCCATTGCATCCGGCGACGGGTCAACCCATTGGCCCCGCCGATCTGGCGCCACTCTTTCCGATGGAGCTGA
TTAAGCAAGAGGTGAGTACCGAGCGCGCTATCCCAATTCCCGATGAGGTACGGGCCATCTATCGACAGTGGCGGCCAACA
CCGCTCTTCCGTGCGCGCCGGTTAGAGAAAGCCCTTGATACGCCGGCTCGTATCTACTACAAGTACGAAGGGGTTAGCCC
TGCCGGTAGCCACAAACCGAATACCGCGGTGGCTCAGGCGTATTACAACAAGCAAGAAGGAGTCCGCCGCCTGGTCACCG
AAACCGGTGCTGGTCAGTGGGGGTCTTCCCTCGCCTTCGCCGGTGCGCTCTTCGGGCTTGAGGTTGAAGTCTTTATGGTG
AAGGTGAGCTACAATCAGAAGCCGTATCGCCGGGCGCTGATGGAAACATACGGCGCACGGGTGGTGGCAAGCCCCAGCAC
CGAGACGGCTGCCGGACGGGCTATTCTGGCGCAAGACCCCGACAGCACCGGGAGTCTTGGTATTGCGATTTCTGAAGCAG
TAGAAGTTGCTGCGCAGCGTGATGATACGCGCTATGCGTTGGGTAGTGTGCTCAACCACGTGCTGCTCCATCAGACGGTG
ATCGGGCAGGAGGCAATGATCCAGATGGAGATGGCCGGTGATTATCCCGATGTGGTGGTTGGCTGTACCGGAGGTGGTTC
AAACTTTGCTGGCATCGCCTTCCCCTTCATCGGTGCGAAGCTGCGCGGTGAACGTCCGTTGCGCGTGGTTGCCGTTGAAC
CGGCAGCCTGTCCGTCGCTGACCAAAGGGAAGTATGCCTATGACTACGGTGATACTGCTCATCTGACACCGCTGGTCAAG
ATGCATACCCTTGGCAGCAGCTTTGTGCCCCCCGGTATTCACGCCGGTGGCCTACGCTATCACGGCATGGCACCGCTGGT
TAGTCATTTGCTTGAAATTGGGGCAATTGAAGCGATTGCGATCCAGCAGCTTGAAACCTTTGCTGCCGGTGTGCAGTTCG
CCCGTACCGAGGGCATCTTGCCTGCCCCTGAAGCAAACCACGCCATTGCCGGTGTGATCCGCGAGGCGCTCCGCTGCAAG
GAAGAGGGTAAGAGCGAGGTTATTCTCTTCAATCTCTGCGGCCACGGCCATTTCGATTTGCAGGCCTACATGGATTATCA
GGCGGGGCGGTTGCGTGACTACGAGTATTCTGACGCCGAGGTGGCAATGGCGCTGGCCGGTTTGCCTTCAGTTGGGGCCT
AA

Upstream 100 bases:

>100_bases
AGCGGCGGCGAACGAGAGTGGCACCGCGGTTTTTCCCGTCTCTCGACCAGTTCGGTCGAGAGATTTTTTTATTCGTCTGT
TGCAGCAAGGAGAGGTGTCT

Downstream 100 bases:

>100_bases
GCTGATTAGCCGCGAGGCGCATGGTTGTGCGCCTCGCGGTAGCAGGGGTACAGCGGGTACTAGCGACGGGGTTTCAGCGT
GGCCGCGACGTTGATGATTG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
METVKYLLSEDRLPETWYNIAADLPSLPPPPLHPATGQPIGPADLAPLFPMELIKQEVSTERAIPIPDEVRAIYRQWRPT
PLFRARRLEKALDTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQEGVRRLVTETGAGQWGSSLAFAGALFGLEVEVFMV
KVSYNQKPYRRALMETYGARVVASPSTETAAGRAILAQDPDSTGSLGIAISEAVEVAAQRDDTRYALGSVLNHVLLHQTV
IGQEAMIQMEMAGDYPDVVVGCTGGGSNFAGIAFPFIGAKLRGERPLRVVAVEPAACPSLTKGKYAYDYGDTAHLTPLVK
MHTLGSSFVPPGIHAGGLRYHGMAPLVSHLLEIGAIEAIAIQQLETFAAGVQFARTEGILPAPEANHAIAGVIREALRCK
EEGKSEVILFNLCGHGHFDLQAYMDYQAGRLRDYEYSDAEVAMALAGLPSVGA

Sequences:

>Translated_453_residues
METVKYLLSEDRLPETWYNIAADLPSLPPPPLHPATGQPIGPADLAPLFPMELIKQEVSTERAIPIPDEVRAIYRQWRPT
PLFRARRLEKALDTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQEGVRRLVTETGAGQWGSSLAFAGALFGLEVEVFMV
KVSYNQKPYRRALMETYGARVVASPSTETAAGRAILAQDPDSTGSLGIAISEAVEVAAQRDDTRYALGSVLNHVLLHQTV
IGQEAMIQMEMAGDYPDVVVGCTGGGSNFAGIAFPFIGAKLRGERPLRVVAVEPAACPSLTKGKYAYDYGDTAHLTPLVK
MHTLGSSFVPPGIHAGGLRYHGMAPLVSHLLEIGAIEAIAIQQLETFAAGVQFARTEGILPAPEANHAIAGVIREALRCK
EEGKSEVILFNLCGHGHFDLQAYMDYQAGRLRDYEYSDAEVAMALAGLPSVGA
>Mature_453_residues
METVKYLLSEDRLPETWYNIAADLPSLPPPPLHPATGQPIGPADLAPLFPMELIKQEVSTERAIPIPDEVRAIYRQWRPT
PLFRARRLEKALDTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQEGVRRLVTETGAGQWGSSLAFAGALFGLEVEVFMV
KVSYNQKPYRRALMETYGARVVASPSTETAAGRAILAQDPDSTGSLGIAISEAVEVAAQRDDTRYALGSVLNHVLLHQTV
IGQEAMIQMEMAGDYPDVVVGCTGGGSNFAGIAFPFIGAKLRGERPLRVVAVEPAACPSLTKGKYAYDYGDTAHLTPLVK
MHTLGSSFVPPGIHAGGLRYHGMAPLVSHLLEIGAIEAIAIQQLETFAAGVQFARTEGILPAPEANHAIAGVIREALRCK
EEGKSEVILFNLCGHGHFDLQAYMDYQAGRLRDYEYSDAEVAMALAGLPSVGA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=374, Percent_Identity=28.0748663101604, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321412, Length=367, Percent_Identity=29.1553133514986, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 48929; Mature: 48929

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVKYLLSEDRLPETWYNIAADLPSLPPPPLHPATGQPIGPADLAPLFPMELIKQEVST
CCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
ERAIPIPDEVRAIYRQWRPTPLFRARRLEKALDTPARIYYKYEGVSPAGSHKPNTAVAQA
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHH
YYNKQEGVRRLVTETGAGQWGSSLAFAGALFGLEVEVFMVKVSYNQKPYRRALMETYGAR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEEEEEECCCCHHHHHHHHHHCCE
VVASPSTETAAGRAILAQDPDSTGSLGIAISEAVEVAAQRDDTRYALGSVLNHVLLHQTV
EEECCCCCHHCCCEEEECCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IGQEAMIQMEMAGDYPDVVVGCTGGGSNFAGIAFPFIGAKLRGERPLRVVAVEPAACPSL
CCHHHEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEECCCCCEEEEEECCCCCCCC
TKGKYAYDYGDTAHLTPLVKMHTLGSSFVPPGIHAGGLRYHGMAPLVSHLLEIGAIEAIA
CCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHH
IQQLETFAAGVQFARTEGILPAPEANHAIAGVIREALRCKEEGKSEVILFNLCGHGHFDL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCH
QAYMDYQAGRLRDYEYSDAEVAMALAGLPSVGA
HEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
METVKYLLSEDRLPETWYNIAADLPSLPPPPLHPATGQPIGPADLAPLFPMELIKQEVST
CCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
ERAIPIPDEVRAIYRQWRPTPLFRARRLEKALDTPARIYYKYEGVSPAGSHKPNTAVAQA
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHH
YYNKQEGVRRLVTETGAGQWGSSLAFAGALFGLEVEVFMVKVSYNQKPYRRALMETYGAR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEEEEEECCCCHHHHHHHHHHCCE
VVASPSTETAAGRAILAQDPDSTGSLGIAISEAVEVAAQRDDTRYALGSVLNHVLLHQTV
EEECCCCCHHCCCEEEECCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IGQEAMIQMEMAGDYPDVVVGCTGGGSNFAGIAFPFIGAKLRGERPLRVVAVEPAACPSL
CCHHHEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEECCCCCEEEEEECCCCCCCC
TKGKYAYDYGDTAHLTPLVKMHTLGSSFVPPGIHAGGLRYHGMAPLVSHLLEIGAIEAIA
CCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHH
IQQLETFAAGVQFARTEGILPAPEANHAIAGVIREALRCKEEGKSEVILFNLCGHGHFDL
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCH
QAYMDYQAGRLRDYEYSDAEVAMALAGLPSVGA
HEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]