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The map label for this gene is trpE [H]

Identifier: 222524802

GI number: 222524802

Start: 1948172

End: 1949683

Strand: Reverse

Name: trpE [H]

Synonym: Chy400_1529

Alternate gene names: 222524802

Gene position: 1949683-1948172 (Counterclockwise)

Preceding gene: 222524803

Following gene: 222524801

Centisome position: 37.0

GC content: 56.94

Gene sequence:

>1512_bases
ATGTACTATCCAACCCTCGATCAATTACAGGAGTTACGCCAGCAGGGTAATCTCTGCCCCATTTATCGCGAGATTATGGC
TGATCTGGAGACGCCAGTATCAGCCTATCTCAAGATCGCTCAGGGTGGGCTGGGCTTTCTCCTCGAAAGTGTGACCGGTG
GTCAGAATATCGGTCGCTACTCGTTCATCGGCAGCGATCCGTATATGGTGCTGCGTATGCACGACGGTGTGGCCCAGGCG
ACCCATGGCGGTTACAAGCAGACCCTCACCTACACCGATCCGTTGATCGTGCTGGAAAGTTATCTGAACGCCTACCGCCC
GATTCGCCTGCCCAATCTACCGATCTTCGTCGGTGGTGCGGTCGGCTATCTCAATTATGAGTCGGCGCGCTACTTCGAGC
GCCTGCCGGTACCTCCAGTGCGCCCATACGATATGCCCGATAGCTGGTGGATGTTTGTTGATACGCTGCTGGCCTTTGAT
CACGTGCGCCACAAGATTATCGTTATTTCGCACGTTCATCTCGATGTCGAAGACCTTGCCGCTGAGTATCAGCGCGCCGT
GCAGCGGATCGAGACCCTGATCGCACGTTTACAACGCCCGCTACCGCCGGGTATTGGCTTGAGCCTGCGCAACTACGAAC
CACACAACACACCGGCGCGGGTGATGACCAACCCTATCGTCTCGAATCGGACCGAGGCCGAATTTAAGGCGGCGGTGCTG
CGGGCGAAAGAGTACATCATGGCCGGCGACATCTTCCAGGTGCAGATTTCGCAGCGCTTCAGCAAAGCCACCAGCGCCGA
TAGCTTCACCATCTATCGGGCATTGCGCACGATCAATCCTTCACCGTACATGTTTTACATCCGTACCGGCGAAGGTGATC
TGGTTGGGGCATCGCCTGAGATGCTGGTACAGGTCCGCGATGGGAAGGTGACCACGCGCCCTATCGCCGGAACCCGCTGG
CGTGGTCGTGATGCGGCTGAAGATGAACAACTGGCTGCCGATCTCCTGGCCGACGAAAAGGAGCGGGCTGAGCATCTGAT
GCTGGTTGATCTGGGACGCAATGATCTGGGACGCATCAGCCAGCCCGGTACGGTTCAGGTGCCGGTGTTCATGACCATCG
AGAAGTATAGCCACGTTCAACATATTGTGTCAGAAGTGACCGGTCTGTTGCGGGCTGACTTGAAACCAATCGATGCGTTG
CGAGCCTGTTTCCCTGCCGGCACCGTGACCGGCGCACCGAAGATCCGGTCGATGGAGATCATTGCCGAACTTGAAGGTGA
GCAGCGGGGGATTTATGCCGGTGCCGTTGGTCATCTCGGTTTTAACGGTGACCTCGATACCTGCATTGCACTCCGCACGC
TGATTGTCAAAGACGGTATTGCCTATGCCCAGGCCGCTGCCGGTGTGGTAGCCGACAGTACGCCGGAGTATGAGTTTAAC
GAAAGCTGTAATAAAGCAGCCGCTTCGTTGCGGGCTATTGATCTGGCCGAAGAGCTACAGGCCGGTGGCTAG

Upstream 100 bases:

>100_bases
TTGGCGCCGAACAGGCACGTGCCGTGGCTGAACCGAAGATGACGCTTATCCTTGAGCGGGTAGGCTTCGTATTACCCAAT
GACCGTAGATAAGGAATTGT

Downstream 100 bases:

>100_bases
AGGCTGGCTCGCAGCTTAGAGTGTTGCACCAGATGAATGTACGTGCCGCAGCTCACGGGGGTGAAAGCGCAACCCTCGCC
CCCGTGAGCTGAAGGCATGA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MYYPTLDQLQELRQQGNLCPIYREIMADLETPVSAYLKIAQGGLGFLLESVTGGQNIGRYSFIGSDPYMVLRMHDGVAQA
THGGYKQTLTYTDPLIVLESYLNAYRPIRLPNLPIFVGGAVGYLNYESARYFERLPVPPVRPYDMPDSWWMFVDTLLAFD
HVRHKIIVISHVHLDVEDLAAEYQRAVQRIETLIARLQRPLPPGIGLSLRNYEPHNTPARVMTNPIVSNRTEAEFKAAVL
RAKEYIMAGDIFQVQISQRFSKATSADSFTIYRALRTINPSPYMFYIRTGEGDLVGASPEMLVQVRDGKVTTRPIAGTRW
RGRDAAEDEQLAADLLADEKERAEHLMLVDLGRNDLGRISQPGTVQVPVFMTIEKYSHVQHIVSEVTGLLRADLKPIDAL
RACFPAGTVTGAPKIRSMEIIAELEGEQRGIYAGAVGHLGFNGDLDTCIALRTLIVKDGIAYAQAAAGVVADSTPEYEFN
ESCNKAAASLRAIDLAEELQAGG

Sequences:

>Translated_503_residues
MYYPTLDQLQELRQQGNLCPIYREIMADLETPVSAYLKIAQGGLGFLLESVTGGQNIGRYSFIGSDPYMVLRMHDGVAQA
THGGYKQTLTYTDPLIVLESYLNAYRPIRLPNLPIFVGGAVGYLNYESARYFERLPVPPVRPYDMPDSWWMFVDTLLAFD
HVRHKIIVISHVHLDVEDLAAEYQRAVQRIETLIARLQRPLPPGIGLSLRNYEPHNTPARVMTNPIVSNRTEAEFKAAVL
RAKEYIMAGDIFQVQISQRFSKATSADSFTIYRALRTINPSPYMFYIRTGEGDLVGASPEMLVQVRDGKVTTRPIAGTRW
RGRDAAEDEQLAADLLADEKERAEHLMLVDLGRNDLGRISQPGTVQVPVFMTIEKYSHVQHIVSEVTGLLRADLKPIDAL
RACFPAGTVTGAPKIRSMEIIAELEGEQRGIYAGAVGHLGFNGDLDTCIALRTLIVKDGIAYAQAAAGVVADSTPEYEFN
ESCNKAAASLRAIDLAEELQAGG
>Mature_503_residues
MYYPTLDQLQELRQQGNLCPIYREIMADLETPVSAYLKIAQGGLGFLLESVTGGQNIGRYSFIGSDPYMVLRMHDGVAQA
THGGYKQTLTYTDPLIVLESYLNAYRPIRLPNLPIFVGGAVGYLNYESARYFERLPVPPVRPYDMPDSWWMFVDTLLAFD
HVRHKIIVISHVHLDVEDLAAEYQRAVQRIETLIARLQRPLPPGIGLSLRNYEPHNTPARVMTNPIVSNRTEAEFKAAVL
RAKEYIMAGDIFQVQISQRFSKATSADSFTIYRALRTINPSPYMFYIRTGEGDLVGASPEMLVQVRDGKVTTRPIAGTRW
RGRDAAEDEQLAADLLADEKERAEHLMLVDLGRNDLGRISQPGTVQVPVFMTIEKYSHVQHIVSEVTGLLRADLKPIDAL
RACFPAGTVTGAPKIRSMEIIAELEGEQRGIYAGAVGHLGFNGDLDTCIALRTLIVKDGIAYAQAAAGVVADSTPEYEFN
ESCNKAAASLRAIDLAEELQAGG

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=421, Percent_Identity=38.479809976247, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1788114, Length=461, Percent_Identity=32.3210412147505, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082077, Length=213, Percent_Identity=31.4553990610329, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786809, Length=269, Percent_Identity=26.3940520446097, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=517, Percent_Identity=41.9729206963249, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324361, Length=406, Percent_Identity=25.3694581280788, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 55762; Mature: 55762

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYPTLDQLQELRQQGNLCPIYREIMADLETPVSAYLKIAQGGLGFLLESVTGGQNIGRY
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCE
SFIGSDPYMVLRMHDGVAQATHGGYKQTLTYTDPLIVLESYLNAYRPIRLPNLPIFVGGA
EEECCCCEEEEEECCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCE
VGYLNYESARYFERLPVPPVRPYDMPDSWWMFVDTLLAFDHVRHKIIVISHVHLDVEDLA
EEEEECHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHH
AEYQRAVQRIETLIARLQRPLPPGIGLSLRNYEPHNTPARVMTNPIVSNRTEAEFKAAVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHCCCHHCCCCHHHHHHHHH
RAKEYIMAGDIFQVQISQRFSKATSADSFTIYRALRTINPSPYMFYIRTGEGDLVGASPE
HHHHHHEECCEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCHH
MLVQVRDGKVTTRPIAGTRWRGRDAAEDEQLAADLLADEKERAEHLMLVDLGRNDLGRIS
EEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCEEEEEECCCCCCCCCC
QPGTVQVPVFMTIEKYSHVQHIVSEVTGLLRADLKPIDALRACFPAGTVTGAPKIRSMEI
CCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHH
IAELEGEQRGIYAGAVGHLGFNGDLDTCIALRTLIVKDGIAYAQAAAGVVADSTPEYEFN
HEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCC
ESCNKAAASLRAIDLAEELQAGG
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYYPTLDQLQELRQQGNLCPIYREIMADLETPVSAYLKIAQGGLGFLLESVTGGQNIGRY
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCE
SFIGSDPYMVLRMHDGVAQATHGGYKQTLTYTDPLIVLESYLNAYRPIRLPNLPIFVGGA
EEECCCCEEEEEECCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCE
VGYLNYESARYFERLPVPPVRPYDMPDSWWMFVDTLLAFDHVRHKIIVISHVHLDVEDLA
EEEEECHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHH
AEYQRAVQRIETLIARLQRPLPPGIGLSLRNYEPHNTPARVMTNPIVSNRTEAEFKAAVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHCCCHHCCCCHHHHHHHHH
RAKEYIMAGDIFQVQISQRFSKATSADSFTIYRALRTINPSPYMFYIRTGEGDLVGASPE
HHHHHHEECCEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCHH
MLVQVRDGKVTTRPIAGTRWRGRDAAEDEQLAADLLADEKERAEHLMLVDLGRNDLGRIS
EEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCEEEEEECCCCCCCCCC
QPGTVQVPVFMTIEKYSHVQHIVSEVTGLLRADLKPIDALRACFPAGTVTGAPKIRSMEI
CCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHH
IAELEGEQRGIYAGAVGHLGFNGDLDTCIALRTLIVKDGIAYAQAAAGVVADSTPEYEFN
HEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCC
ESCNKAAASLRAIDLAEELQAGG
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2732211 [H]