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The map label for this gene is acoA [H]

Identifier: 222524730

GI number: 222524730

Start: 1842925

End: 1843929

Strand: Reverse

Name: acoA [H]

Synonym: Chy400_1457

Alternate gene names: 222524730

Gene position: 1843929-1842925 (Counterclockwise)

Preceding gene: 222524731

Following gene: 222524729

Centisome position: 35.0

GC content: 45.87

Gene sequence:

>1005_bases
ATGGAAATGCTCATGTCTACATCAGAGATTTTCTCTCCCGATCATAGTAAGCTCATCAACGAATTAGGGAATGAACGCTT
GCGTGAATGGGTGCTTAGAATGCAGATTATTCGTGCTTTTGAAGAAAAAGCCGAAGAACTGTTTGCGCGTGGATTAGTAC
ACGGCACGATGCATCTTTCGATCGGGCAGGAGGCTGTGGCGATAGGTGCTTCTGCAGCGATGAAACCTGGAGATTATTTG
CTTAACCATCATCGTGGCCATGGACATTGTCTGGCCTGGGGTAGTGATGTACGGCTAATGATGGCAGAGTTTTTAGGCAA
AGAAACAGGATATTGCCGTGGTCGTGGTGGATCAATGCACATTGCTAATGTTGAAATGAATAATTTGGGTGCAAATGGTA
TTGTTGGTGGTGGTATTCCCATTTCAGTTGGTGTTGGATTGAGTATTAAAAAGCGCCGATCTTCTCAGGTTTGTCTCACT
ATTTTTGGTGATGGTGCAGTCAATACTGGTGCTTTTCACGAATCCCTAAATATGGCCAGTATCTGGAACTTACCCGTGGT
TTATCTCTGTGAGAACAACCAATACGCTATGTCAATGCCGATCCAGAAAGCGTGCAGACTCAATCACCTGAGCCAGCGAG
CTGCTGCATATGCGATAGCTGGAATAACGGTTGATGGTAACGATGCATTAGCAGTGTATGAAGCAGTTCGCCAGGCTGTG
GCAAGAGCGCGAAGTGGTTATGGGCCAACACTTGTTGAAGCGATTACCTATCGTTGGAAAGGTCATTCTAAGAGTGACCG
TCAAGCTTATCGGAGTCGTGATGAGGTGAAAGATTGGCAATCTCGTGATCCAATCATGCGATTAGCCAGACTGATTCAAA
TGAGTGACGCTGAATTCAAAGCTATCGTTGATCAAGCTCGGACTATGATCGAGGAAGCGGTCGAATTTGCTCAGGCCAGT
CCGGAACCTGATCCTGATACTATTTTTGAGGGATTGTATGCGTGA

Upstream 100 bases:

>100_bases
CAAAGTTCATCAATTATTTAGTGACTGATGAAATAGCTGCCCTCCGAATACAGAAGTTTTTTGAGGGTGATGGAACTCTG
TGAGGAGATAAGTCATACTT

Downstream 100 bases:

>100_bases
AATTACTTATGTCGAAGCAATTCGTGAAGCCCTACGGCAGAAGATGAAAGAAGATGAAACGGTTTTTTTGATTGGCGAAG
ATATTGGCTTGTATGGTGGT

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MEMLMSTSEIFSPDHSKLINELGNERLREWVLRMQIIRAFEEKAEELFARGLVHGTMHLSIGQEAVAIGASAAMKPGDYL
LNHHRGHGHCLAWGSDVRLMMAEFLGKETGYCRGRGGSMHIANVEMNNLGANGIVGGGIPISVGVGLSIKKRRSSQVCLT
IFGDGAVNTGAFHESLNMASIWNLPVVYLCENNQYAMSMPIQKACRLNHLSQRAAAYAIAGITVDGNDALAVYEAVRQAV
ARARSGYGPTLVEAITYRWKGHSKSDRQAYRSRDEVKDWQSRDPIMRLARLIQMSDAEFKAIVDQARTMIEEAVEFAQAS
PEPDPDTIFEGLYA

Sequences:

>Translated_334_residues
MEMLMSTSEIFSPDHSKLINELGNERLREWVLRMQIIRAFEEKAEELFARGLVHGTMHLSIGQEAVAIGASAAMKPGDYL
LNHHRGHGHCLAWGSDVRLMMAEFLGKETGYCRGRGGSMHIANVEMNNLGANGIVGGGIPISVGVGLSIKKRRSSQVCLT
IFGDGAVNTGAFHESLNMASIWNLPVVYLCENNQYAMSMPIQKACRLNHLSQRAAAYAIAGITVDGNDALAVYEAVRQAV
ARARSGYGPTLVEAITYRWKGHSKSDRQAYRSRDEVKDWQSRDPIMRLARLIQMSDAEFKAIVDQARTMIEEAVEFAQAS
PEPDPDTIFEGLYA
>Mature_334_residues
MEMLMSTSEIFSPDHSKLINELGNERLREWVLRMQIIRAFEEKAEELFARGLVHGTMHLSIGQEAVAIGASAAMKPGDYL
LNHHRGHGHCLAWGSDVRLMMAEFLGKETGYCRGRGGSMHIANVEMNNLGANGIVGGGIPISVGVGLSIKKRRSSQVCLT
IFGDGAVNTGAFHESLNMASIWNLPVVYLCENNQYAMSMPIQKACRLNHLSQRAAAYAIAGITVDGNDALAVYEAVRQAV
ARARSGYGPTLVEAITYRWKGHSKSDRQAYRSRDEVKDWQSRDPIMRLARLIQMSDAEFKAIVDQARTMIEEAVEFAQAS
PEPDPDTIFEGLYA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=307, Percent_Identity=39.0879478827362, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI4505685, Length=296, Percent_Identity=41.8918918918919, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI291084742, Length=307, Percent_Identity=40.7166123778502, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI291084744, Length=303, Percent_Identity=40.9240924092409, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI291084757, Length=296, Percent_Identity=37.5, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI11386135, Length=280, Percent_Identity=32.8571428571429, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI258645172, Length=280, Percent_Identity=32.8571428571429, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=37.7358490566038, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=37.7358490566038, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI86563355, Length=236, Percent_Identity=33.8983050847458, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI86563357, Length=236, Percent_Identity=33.8983050847458, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321026, Length=341, Percent_Identity=36.3636363636364, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24639744, Length=321, Percent_Identity=36.4485981308411, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28571106, Length=321, Percent_Identity=36.4485981308411, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24639740, Length=321, Percent_Identity=36.4485981308411, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24639746, Length=308, Percent_Identity=37.6623376623377, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24639748, Length=320, Percent_Identity=35.625, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21355903, Length=323, Percent_Identity=26.9349845201238, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36845; Mature: 36845

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLMSTSEIFSPDHSKLINELGNERLREWVLRMQIIRAFEEKAEELFARGLVHGTMHLS
CCCHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
IGQEAVAIGASAAMKPGDYLLNHHRGHGHCLAWGSDVRLMMAEFLGKETGYCRGRGGSMH
CCCHHHHHCCHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEECCCCCEE
IANVEMNNLGANGIVGGGIPISVGVGLSIKKRRSSQVCLTIFGDGAVNTGAFHESLNMAS
EEEEEECCCCCCCEECCCEEEEECCCCEEECCCCCCEEEEEECCCCCCCCHHHHCCCHHE
IWNLPVVYLCENNQYAMSMPIQKACRLNHLSQRAAAYAIAGITVDGNDALAVYEAVRQAV
EECCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHH
ARARSGYGPTLVEAITYRWKGHSKSDRQAYRSRDEVKDWQSRDPIMRLARLIQMSDAEFK
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
AIVDQARTMIEEAVEFAQASPEPDPDTIFEGLYA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MEMLMSTSEIFSPDHSKLINELGNERLREWVLRMQIIRAFEEKAEELFARGLVHGTMHLS
CCCHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
IGQEAVAIGASAAMKPGDYLLNHHRGHGHCLAWGSDVRLMMAEFLGKETGYCRGRGGSMH
CCCHHHHHCCHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEECCCCCEE
IANVEMNNLGANGIVGGGIPISVGVGLSIKKRRSSQVCLTIFGDGAVNTGAFHESLNMAS
EEEEEECCCCCCCEECCCEEEEECCCCEEECCCCCCEEEEEECCCCCCCCHHHHCCCHHE
IWNLPVVYLCENNQYAMSMPIQKACRLNHLSQRAAAYAIAGITVDGNDALAVYEAVRQAV
EECCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHH
ARARSGYGPTLVEAITYRWKGHSKSDRQAYRSRDEVKDWQSRDPIMRLARLIQMSDAEFK
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
AIVDQARTMIEEAVEFAQASPEPDPDTIFEGLYA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]