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The map label for this gene is kipI [H]

Identifier: 222524709

GI number: 222524709

Start: 1824677

End: 1825321

Strand: Reverse

Name: kipI [H]

Synonym: Chy400_1436

Alternate gene names: 222524709

Gene position: 1825321-1824677 (Counterclockwise)

Preceding gene: 222524710

Following gene: 222524708

Centisome position: 34.64

GC content: 61.71

Gene sequence:

>645_bases
ATGAGCGGACGGGTAGATCCATTTGGCGAAGCAGCGTTCCTGGTGCGCTGGCCGACCGATCTGGCCGGCGCAGGGGCACC
GCTGGCCGCAATGGCTGCACTCCAATCCGATCCACCCCCCGGTTTGATCGATCTGGTGCCGGCAATTGACTCGCTACTGG
TCTGTTTCGATCCGTTGATCGCCAATCGTGATCTTCTTGCCGCCCGTCTGGCCGATCTGGCTGCACATCCACCCTTAACC
GCGATGCCCGTCGGACGTGAGGTGGTTATTCCGGTGCGTTACGGCGGCGATGAGGGGCCTGATCTCCACGAGGTCGCTGC
CCAATCCGGGTTGTCACCGGATGAGGTAGTGGCCTTACATACCGGTACCGTACAGCGTGTGCTGATGATCGGTTTTGCTC
CGGGCTACCCTTATCTGGGCTGGTTACCGCCAGCCCTTCATCTGCCACGGCGGGCAACGCCGCGCCTGGCAGTTCCCGCC
GGATCGGTGGCAATTGCTGCCGGTATGACCGGTATCTATCCGACAAAGTTGCCCGGCGGCTGGCATATCATCGGTCGCAC
CGGCCTGCGATTGTTCAATCCGACTGCTGATCCACCGACGCTGTTGCAACCGGGTGATCGGGTGCGCTTTGTGCGAGAAG
AGTGA

Upstream 100 bases:

>100_bases
CAATTTGTATCCACGGCGATACACCGGGTGCCGCGCTGCGAGCAGCAACGATCCGTCAGGGTCTGGCTGCCGCAGGCATC
ACCGTACAGGCGCCACAACG

Downstream 100 bases:

>100_bases
CCAACGCCGGTATTGGGCATCCTCTTCGCCAGTGTATCTTCGGGTAGGGGCATGGCATGCCGTGTCCCTACCGCATCAGG
TTGATCTGGATTTGCACCAA

Product: Allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSGRVDPFGEAAFLVRWPTDLAGAGAPLAAMAALQSDPPPGLIDLVPAIDSLLVCFDPLIANRDLLAARLADLAAHPPLT
AMPVGREVVIPVRYGGDEGPDLHEVAAQSGLSPDEVVALHTGTVQRVLMIGFAPGYPYLGWLPPALHLPRRATPRLAVPA
GSVAIAAGMTGIYPTKLPGGWHIIGRTGLRLFNPTADPPTLLQPGDRVRFVREE

Sequences:

>Translated_214_residues
MSGRVDPFGEAAFLVRWPTDLAGAGAPLAAMAALQSDPPPGLIDLVPAIDSLLVCFDPLIANRDLLAARLADLAAHPPLT
AMPVGREVVIPVRYGGDEGPDLHEVAAQSGLSPDEVVALHTGTVQRVLMIGFAPGYPYLGWLPPALHLPRRATPRLAVPA
GSVAIAAGMTGIYPTKLPGGWHIIGRTGLRLFNPTADPPTLLQPGDRVRFVREE
>Mature_213_residues
SGRVDPFGEAAFLVRWPTDLAGAGAPLAAMAALQSDPPPGLIDLVPAIDSLLVCFDPLIANRDLLAARLADLAAHPPLTA
MPVGREVVIPVRYGGDEGPDLHEVAAQSGLSPDEVVALHTGTVQRVLMIGFAPGYPYLGWLPPALHLPRRATPRLAVPAG
SVAIAAGMTGIYPTKLPGGWHIIGRTGLRLFNPTADPPTLLQPGDRVRFVREE

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=129, Percent_Identity=58.1395348837209, Blast_Score=144, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 22513; Mature: 22382

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRVDPFGEAAFLVRWPTDLAGAGAPLAAMAALQSDPPPGLIDLVPAIDSLLVCFDPLI
CCCCCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ANRDLLAARLADLAAHPPLTAMPVGREVVIPVRYGGDEGPDLHEVAAQSGLSPDEVVALH
CCCHHHHHHHHHHHCCCCCEEECCCCEEEEEEEECCCCCCCHHHHHHHCCCCHHHEEEEE
TGTVQRVLMIGFAPGYPYLGWLPPALHLPRRATPRLAVPAGSVAIAAGMTGIYPTKLPGG
CCHHHEEEEEECCCCCCCCCCCCHHHCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCC
WHIIGRTGLRLFNPTADPPTLLQPGDRVRFVREE
EEEEECCCEEEECCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SGRVDPFGEAAFLVRWPTDLAGAGAPLAAMAALQSDPPPGLIDLVPAIDSLLVCFDPLI
CCCCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ANRDLLAARLADLAAHPPLTAMPVGREVVIPVRYGGDEGPDLHEVAAQSGLSPDEVVALH
CCCHHHHHHHHHHHCCCCCEEECCCCEEEEEEEECCCCCCCHHHHHHHCCCCHHHEEEEE
TGTVQRVLMIGFAPGYPYLGWLPPALHLPRRATPRLAVPAGSVAIAAGMTGIYPTKLPGG
CCHHHEEEEEECCCCCCCCCCCCHHHCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCC
WHIIGRTGLRLFNPTADPPTLLQPGDRVRFVREE
EEEEECCCEEEECCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]