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The map label for this gene is pyrG [H]

Identifier: 222524505

GI number: 222524505

Start: 1563705

End: 1565345

Strand: Reverse

Name: pyrG [H]

Synonym: Chy400_1229

Alternate gene names: 222524505

Gene position: 1565345-1563705 (Counterclockwise)

Preceding gene: 222524506

Following gene: 222524503

Centisome position: 29.71

GC content: 56.86

Gene sequence:

>1641_bases
ATGACAAAGTACATTTTTGTGACAGGTGGTGTGGTTTCATCCGTGGGGAAGGGCATTGGTGTCGCCTCCATTGGCCGCCT
GCTGAAGAGTCGTGGCCTTTCAGTTTCGGTTATGAAGCTCGATCCGTACCTGAATGTCGATCCGGGTACGATGTCACCGT
ATCAGCATGGTGAGGTCTTTGTCACGGCTGATGGAGCTGAAACCGATCTCGATTTAGGTCACTATGAACGATTCATCGAT
GTCAATCTCAGTCGGCTGAGCAACGTCACCACCGGTCAGATCTACTCGGCGGTTATCGCGAAAGAGCGGCGGGGTGATTA
CCTTGGCGGAACGATTCAGGTTATTCCGCATATCACCAACGAGATCAAGTCGCGGATTGGGTCACTGGCCAGGAGCAGTC
AGGCCGATGTAGTGATTGTCGAAATTGGTGGTACAGTTGGCGACATTGAAAGCCTGCCGTTTCTGGAAGCCATCCGCCAA
ATGCGTAAGGATGTCGGGCGCGACAATATCCTTTACATTCACGTAACGCTACTGCCACATATCAGCACTGGCGAACTTAA
GACAAAACCGACCCAACACTCGGTGATGGCGCTGCGCAACGTTGGAATTAGCGCCGACATCATCCTCTGCCGGGCCGACC
GTCCGATTGACGATGAGATTCGCGAGAAGATTGCCTTCTTCGCCGACGTGGACGTGCGGGCTGTCATTCCGGTGCCGACG
GTCGACTCGATCTACGAAGTACCACTGGTGCTGGAAGATATGGGATTAGGCGATTATCTGGTCGAACGGTTAGGGTTACC
GGCTACCCCGCCCGATCTCGAAGAGTGGCGCGCACTCGTTGCCCGCATCCGTCAGGAAAAGCGGCGGGTGCCAATCGCAC
TGGTCGGCAAATATGTCGAGCTGCACGATGCCTATATCAGTGTCGTTGAGGCACTGCACCACGCCGGTCTCGAACAATCG
ATTGATATTGACATTCGTTGGATTGCCGCCGAGGAAGTGGAACGGGAAGGGCCGGCGCGCTTGCTGTCGGGAGTGTATGG
GATACTGGTGCCGGGTGGTTTCGGCGAACGAGGGATCGAAGGCAAGATTGCGGCTGCTGACTACGCCCGGATTCACGGCA
TCCCCTATCTGGGTCTTTGTCTGGGGATGCAATGCGCCACCATCGCCTTTGCCCGCCATGTCCTCGGCACCCACGACGTC
AACAGCACCGAATTCAACCCCCAGACTGCCCATCCGGTGATTGATCTGATGCCCGATCAGCGCGACATTACCGAAAAGGG
TGGCACCATGCGGCTGGGTTTGTACCCATGTGATTTGGTACCGGGGACGCGAGCGCATGCCGCTTATGGCTGCGACCGGG
TAGAAGAACGCCATCGCCATCGGTTTGAGTTTAACAATCGCTATCGCAGCGTGCTCGAAGCTGCCGGTCTGGTGATCAGT
GGTATCTCACCCGATAAGCGGTTGGTCGAGATCATCGAACTTCGCGACCATCCCTGGTACGTTGCCAGTCAGTTCCATCC
CGAATTTCAGTCCCGGCCCGGCAAACCGCACCCGCTCTTCCGCGGCTTTGTAGCGGCAGCCGCGCAGACACTCCTCGCCG
GCGAAGCGCGCCAGTTGCCGCTGGTGGAAAGCACGTCGTAG

Upstream 100 bases:

>100_bases
TGCAATCGTCCTGGATACAAGTGAAACGAGTACCGGTGTGGGTCGGTTCAACCCGAAGGTTGACCGACCCACAAATCTTT
AAGACGGGGAGTACTGCTCT

Downstream 100 bases:

>100_bases
TGCGGAAGCACAGACAGCGGATATGTAGCGTCGGTTTGGAGGTGCGGGAGCATAGCTTCCGCACCTGGTGTGTTTTGTCA
CACCAGCCTCCTTTGAACGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTKYIFVTGGVVSSVGKGIGVASIGRLLKSRGLSVSVMKLDPYLNVDPGTMSPYQHGEVFVTADGAETDLDLGHYERFID
VNLSRLSNVTTGQIYSAVIAKERRGDYLGGTIQVIPHITNEIKSRIGSLARSSQADVVIVEIGGTVGDIESLPFLEAIRQ
MRKDVGRDNILYIHVTLLPHISTGELKTKPTQHSVMALRNVGISADIILCRADRPIDDEIREKIAFFADVDVRAVIPVPT
VDSIYEVPLVLEDMGLGDYLVERLGLPATPPDLEEWRALVARIRQEKRRVPIALVGKYVELHDAYISVVEALHHAGLEQS
IDIDIRWIAAEEVEREGPARLLSGVYGILVPGGFGERGIEGKIAAADYARIHGIPYLGLCLGMQCATIAFARHVLGTHDV
NSTEFNPQTAHPVIDLMPDQRDITEKGGTMRLGLYPCDLVPGTRAHAAYGCDRVEERHRHRFEFNNRYRSVLEAAGLVIS
GISPDKRLVEIIELRDHPWYVASQFHPEFQSRPGKPHPLFRGFVAAAAQTLLAGEARQLPLVESTS

Sequences:

>Translated_546_residues
MTKYIFVTGGVVSSVGKGIGVASIGRLLKSRGLSVSVMKLDPYLNVDPGTMSPYQHGEVFVTADGAETDLDLGHYERFID
VNLSRLSNVTTGQIYSAVIAKERRGDYLGGTIQVIPHITNEIKSRIGSLARSSQADVVIVEIGGTVGDIESLPFLEAIRQ
MRKDVGRDNILYIHVTLLPHISTGELKTKPTQHSVMALRNVGISADIILCRADRPIDDEIREKIAFFADVDVRAVIPVPT
VDSIYEVPLVLEDMGLGDYLVERLGLPATPPDLEEWRALVARIRQEKRRVPIALVGKYVELHDAYISVVEALHHAGLEQS
IDIDIRWIAAEEVEREGPARLLSGVYGILVPGGFGERGIEGKIAAADYARIHGIPYLGLCLGMQCATIAFARHVLGTHDV
NSTEFNPQTAHPVIDLMPDQRDITEKGGTMRLGLYPCDLVPGTRAHAAYGCDRVEERHRHRFEFNNRYRSVLEAAGLVIS
GISPDKRLVEIIELRDHPWYVASQFHPEFQSRPGKPHPLFRGFVAAAAQTLLAGEARQLPLVESTS
>Mature_545_residues
TKYIFVTGGVVSSVGKGIGVASIGRLLKSRGLSVSVMKLDPYLNVDPGTMSPYQHGEVFVTADGAETDLDLGHYERFIDV
NLSRLSNVTTGQIYSAVIAKERRGDYLGGTIQVIPHITNEIKSRIGSLARSSQADVVIVEIGGTVGDIESLPFLEAIRQM
RKDVGRDNILYIHVTLLPHISTGELKTKPTQHSVMALRNVGISADIILCRADRPIDDEIREKIAFFADVDVRAVIPVPTV
DSIYEVPLVLEDMGLGDYLVERLGLPATPPDLEEWRALVARIRQEKRRVPIALVGKYVELHDAYISVVEALHHAGLEQSI
DIDIRWIAAEEVEREGPARLLSGVYGILVPGGFGERGIEGKIAAADYARIHGIPYLGLCLGMQCATIAFARHVLGTHDVN
STEFNPQTAHPVIDLMPDQRDITEKGGTMRLGLYPCDLVPGTRAHAAYGCDRVEERHRHRFEFNNRYRSVLEAAGLVISG
ISPDKRLVEIIELRDHPWYVASQFHPEFQSRPGKPHPLFRGFVAAAAQTLLAGEARQLPLVESTS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=47.7313974591652, Blast_Score=499, Evalue=1e-141,
Organism=Homo sapiens, GI28559085, Length=553, Percent_Identity=46.2929475587703, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI28559083, Length=553, Percent_Identity=46.2929475587703, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI221316689, Length=553, Percent_Identity=46.2929475587703, Blast_Score=479, Evalue=1e-135,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=54.7309833024119, Blast_Score=574, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25148299, Length=606, Percent_Identity=40.5940594059406, Blast_Score=425, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=561, Percent_Identity=44.7415329768271, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322563, Length=564, Percent_Identity=44.8581560283688, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24664469, Length=551, Percent_Identity=48.2758620689655, Blast_Score=490, Evalue=1e-138,
Organism=Drosophila melanogaster, GI21357815, Length=496, Percent_Identity=46.5725806451613, Blast_Score=415, Evalue=1e-116,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60070; Mature: 59939

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSVGKGIGVASIGRLLKSRGLSVSVMKLDPYLNVDPGTMSPYQHGEVF
CCEEEEEECCHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCCCEEE
VTADGAETDLDLGHYERFIDVNLSRLSNVTTGQIYSAVIAKERRGDYLGGTIQVIPHITN
EEECCCCCCCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
EIKSRIGSLARSSQADVVIVEIGGTVGDIESLPFLEAIRQMRKDVGRDNILYIHVTLLPH
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECC
ISTGELKTKPTQHSVMALRNVGISADIILCRADRPIDDEIREKIAFFADVDVRAVIPVPT
CCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
VDSIYEVPLVLEDMGLGDYLVERLGLPATPPDLEEWRALVARIRQEKRRVPIALVGKYVE
HHHHHHCCEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LHDAYISVVEALHHAGLEQSIDIDIRWIAAEEVEREGPARLLSGVYGILVPGGFGERGIE
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHCCCCHHHHHHCHHHEEECCCCCCCCCC
GKIAAADYARIHGIPYLGLCLGMQCATIAFARHVLGTHDVNSTEFNPQTAHPVIDLMPDQ
CEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCC
RDITEKGGTMRLGLYPCDLVPGTRAHAAYGCDRVEERHRHRFEFNNRYRSVLEAAGLVIS
HHHHHCCCEEEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHCCHHHHHHHHHHHCCEEEE
GISPDKRLVEIIELRDHPWYVASQFHPEFQSRPGKPHPLFRGFVAAAAQTLLAGEARQLP
CCCCHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
LVESTS
CCCCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSVGKGIGVASIGRLLKSRGLSVSVMKLDPYLNVDPGTMSPYQHGEVF
CEEEEEECCHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCCCEEE
VTADGAETDLDLGHYERFIDVNLSRLSNVTTGQIYSAVIAKERRGDYLGGTIQVIPHITN
EEECCCCCCCCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
EIKSRIGSLARSSQADVVIVEIGGTVGDIESLPFLEAIRQMRKDVGRDNILYIHVTLLPH
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECC
ISTGELKTKPTQHSVMALRNVGISADIILCRADRPIDDEIREKIAFFADVDVRAVIPVPT
CCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
VDSIYEVPLVLEDMGLGDYLVERLGLPATPPDLEEWRALVARIRQEKRRVPIALVGKYVE
HHHHHHCCEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LHDAYISVVEALHHAGLEQSIDIDIRWIAAEEVEREGPARLLSGVYGILVPGGFGERGIE
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHCCCCHHHHHHCHHHEEECCCCCCCCCC
GKIAAADYARIHGIPYLGLCLGMQCATIAFARHVLGTHDVNSTEFNPQTAHPVIDLMPDQ
CEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCC
RDITEKGGTMRLGLYPCDLVPGTRAHAAYGCDRVEERHRHRFEFNNRYRSVLEAAGLVIS
HHHHHCCCEEEEEEEECCCCCCCCCHHHHCHHHHHHHHHHHCCHHHHHHHHHHHCCEEEE
GISPDKRLVEIIELRDHPWYVASQFHPEFQSRPGKPHPLFRGFVAAAAQTLLAGEARQLP
CCCCHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
LVESTS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA