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The map label for this gene is sigB [H]

Identifier: 222524178

GI number: 222524178

Start: 1132032

End: 1132973

Strand: Direct

Name: sigB [H]

Synonym: Chy400_0897

Alternate gene names: 222524178

Gene position: 1132032-1132973 (Clockwise)

Preceding gene: 222524177

Following gene: 222524179

Centisome position: 21.48

GC content: 62.0

Gene sequence:

>942_bases
ATGAGGAACCCTACCACGATTGGCCGCAGTGCATCTGACCCAATAGTGCTCTACCTGAACGAAATCGGCGCCGAGCCGTT
GCTGACCTTCGAGCAGGAACAGCACCTGGCCCAAACTATGAGTCAGGGTCGGCGAGCAGCCGAGCGCTTGCACAATGAGC
CACTGCTTCCCCTGGCTGAAAAGCAACGGCTCATAGCCCAGGTCAATGCCGGGGAACAGGCCCGTGCCCAACTGATCAAC
TCAAACCTGCGTCTGGTTGTGAGCATCGCCCGCCGTTATCAGGGTCACGGCCTGAGCCTGCTCGATCTGATCCAGGAGGG
GAGTGTTGGCCTGATGCGCGCCGTTGACAAGTTTGACGCCAGTCGTGGGCTGAAGTTTTCCACCTACGCCACGTACTGGA
TTCGGCAGAGCATTGGCAGAGCCATTGCTGACCATAGTCGCACCGTGCGCCTCCCCGTTCATCTCGGCGAACGGCTCAGC
CGGCTTTCGCGTCTGCGCCAGGAACTCTCGCAACAGCTCGACCGCGAACCAACCCTGGAAGAGCTGGCTCAGGCTGCCGG
CCTGAGCGTAGAGCAGGTCGAACGGGCCGAACAGGCCGGTCTGGCGCCGACCTCACTCGATGAACCGCATACCGAAGATG
GCGGTGGCGCACTGGCCGAAATCCTGACCGATCCGCTCCAGCCCACCCCCTTCGACGAAGTGAGCGAGAGCATGCTGCAA
GCCGATGTCCAGCACGCCCTGCAACAGCTTTCGCCGCGCGAACGCAGCATCCTACGCCTGCGCTACGGCCTGGACGGCGA
GCCGATGCACACCCTCGAACAGATCGGACAGCGCCTCCGCCTGACCCGCGAGCGCGTGCGCCAGTTGGAACACGAAGCGC
TCCGCAAACTGCGCGATCCATCCCTGCACCCACAATTGCAGGGTTATGTGAACGAAGAATAA

Upstream 100 bases:

>100_bases
CCGTGGGGAACCACCGAGGCACCTGCTGACGTCTCATAAGTACCCCTCACGGATCACATGACAGACATCGGCAGTGTTCA
CCGATGTCTAAGGAGGTAGA

Downstream 100 bases:

>100_bases
TCATCCAGCGCAGCAATCACCCCGCTACAGGCGCGTCGTTGGCCAACGGCGCGCCGTGTCATATTGAGAGTGGTGATAGC
CGTACAAATTCCCTCCATCC

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRNPTTIGRSASDPIVLYLNEIGAEPLLTFEQEQHLAQTMSQGRRAAERLHNEPLLPLAEKQRLIAQVNAGEQARAQLIN
SNLRLVVSIARRYQGHGLSLLDLIQEGSVGLMRAVDKFDASRGLKFSTYATYWIRQSIGRAIADHSRTVRLPVHLGERLS
RLSRLRQELSQQLDREPTLEELAQAAGLSVEQVERAEQAGLAPTSLDEPHTEDGGGALAEILTDPLQPTPFDEVSESMLQ
ADVQHALQQLSPRERSILRLRYGLDGEPMHTLEQIGQRLRLTRERVRQLEHEALRKLRDPSLHPQLQGYVNEE

Sequences:

>Translated_313_residues
MRNPTTIGRSASDPIVLYLNEIGAEPLLTFEQEQHLAQTMSQGRRAAERLHNEPLLPLAEKQRLIAQVNAGEQARAQLIN
SNLRLVVSIARRYQGHGLSLLDLIQEGSVGLMRAVDKFDASRGLKFSTYATYWIRQSIGRAIADHSRTVRLPVHLGERLS
RLSRLRQELSQQLDREPTLEELAQAAGLSVEQVERAEQAGLAPTSLDEPHTEDGGGALAEILTDPLQPTPFDEVSESMLQ
ADVQHALQQLSPRERSILRLRYGLDGEPMHTLEQIGQRLRLTRERVRQLEHEALRKLRDPSLHPQLQGYVNEE
>Mature_313_residues
MRNPTTIGRSASDPIVLYLNEIGAEPLLTFEQEQHLAQTMSQGRRAAERLHNEPLLPLAEKQRLIAQVNAGEQARAQLIN
SNLRLVVSIARRYQGHGLSLLDLIQEGSVGLMRAVDKFDASRGLKFSTYATYWIRQSIGRAIADHSRTVRLPVHLGERLS
RLSRLRQELSQQLDREPTLEELAQAAGLSVEQVERAEQAGLAPTSLDEPHTEDGGGALAEILTDPLQPTPFDEVSESMLQ
ADVQHALQQLSPRERSILRLRYGLDGEPMHTLEQIGQRLRLTRERVRQLEHEALRKLRDPSLHPQLQGYVNEE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=294, Percent_Identity=38.7755102040816, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=42.1602787456446, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789871, Length=298, Percent_Identity=28.5234899328859, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788231, Length=205, Percent_Identity=29.7560975609756, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35243; Mature: 35243

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNPTTIGRSASDPIVLYLNEIGAEPLLTFEQEQHLAQTMSQGRRAAERLHNEPLLPLAE
CCCCCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
KQRLIAQVNAGEQARAQLINSNLRLVVSIARRYQGHGLSLLDLIQEGSVGLMRAVDKFDA
HHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHH
SRGLKFSTYATYWIRQSIGRAIADHSRTVRLPVHLGERLSRLSRLRQELSQQLDREPTLE
HCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELAQAAGLSVEQVERAEQAGLAPTSLDEPHTEDGGGALAEILTDPLQPTPFDEVSESMLQ
HHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
ADVQHALQQLSPRERSILRLRYGLDGEPMHTLEQIGQRLRLTRERVRQLEHEALRKLRDP
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLHPQLQGYVNEE
CCCCHHCCCCCCC
>Mature Secondary Structure
MRNPTTIGRSASDPIVLYLNEIGAEPLLTFEQEQHLAQTMSQGRRAAERLHNEPLLPLAE
CCCCCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
KQRLIAQVNAGEQARAQLINSNLRLVVSIARRYQGHGLSLLDLIQEGSVGLMRAVDKFDA
HHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHH
SRGLKFSTYATYWIRQSIGRAIADHSRTVRLPVHLGERLSRLSRLRQELSQQLDREPTLE
HCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELAQAAGLSVEQVERAEQAGLAPTSLDEPHTEDGGGALAEILTDPLQPTPFDEVSESMLQ
HHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
ADVQHALQQLSPRERSILRLRYGLDGEPMHTLEQIGQRLRLTRERVRQLEHEALRKLRDP
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLHPQLQGYVNEE
CCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]