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The map label for this gene is appA [H]

Identifier: 222524134

GI number: 222524134

Start: 1072135

End: 1073976

Strand: Direct

Name: appA [H]

Synonym: Chy400_0850

Alternate gene names: 222524134

Gene position: 1072135-1073976 (Clockwise)

Preceding gene: 222524130

Following gene: 222524135

Centisome position: 20.35

GC content: 58.96

Gene sequence:

>1842_bases
GTGTCGCACAAACGACTTTGGGTATGGATGACCCTCGTAGCAGTGGCGGCAATGGTCCTGGCAGCGTGTGGTACGCAACA
ACCGCAGGCACAACCGACCACAGCACCGGCAGCGCAGGCAACTGCTACCCCGCAGCCGACTGCTGCCCCACAGCCAACTG
CTGCACCGGCAGCTACAACTGCACCGGCCCCAACCGAGGCCCCAACCGCCGCGCCGCAGCAGGGTGGTAAGCTGACGATC
CTGTACTGGCAGGCAGTTACGACGCTTAATCCGCACCTGGCAACCGGTACCAAGGACTTCGACGGTGCAACGGTGATTCT
CGAACCGCTGGCCCGCTACAACGAGAAAGACGAGTTGGTGCCGTTCCTGGCCGCTGAAATCCCAACGCTGGAGAATGGCG
GGGTTGCCGCTGATGGTAGCAGCGTGACCTGGAAGATCAAGCCGGGTCTGAAGTGGTCGGACGGGAGTGACTTCACGATT
GACGATATTATCTTCACCTGGCAGTACTGCGCCGATGAAGCCACGGCCTGTACGACCAAGGCGGCCTTCGATCCAATTGC
GAACATCGAGAAGATTGACGACACGACCTTCCGGATCATCTGGAAAGAGCCGAACGCCAACCCCTACATCTCGTTCGTTG
GCCCGCTCGGTATGATCTTGCAGAAGAAGCAGTTCGAGAACTGTATCGGTGCTGCGGCCAGCACCAGTGCTGAATGCCAG
GCGGCTAATCTGGCACCGATTGGCACCAATGCCTGGAAGCTGAAGGAATTCCGCCCCGGTGACACGGTAATCTACGAGCG
GAACCCCTTCTACCGTGATGCTGCCAATGTCTTCTTCGATGAGGTCGAGATTAAGGGTGGTGGTGATGCCACGTCGGCAG
CACGGGCAGTCTGTGAAACGGCTGAGGTCGACTTTGCCTGGAACTTGCAGATTCCGAAGGCCGTGCTCGAACCAATTCTG
GCTGCCGGTAATTGTGATGCTATCGCCGGTGGTTCGTTCGGTGTCGAGCGGATTGTGGTCAACTTCGCTAACCCTGACCC
GGCCCTGGGTGACAAGCGCAGCGAACCCGATCAGCCGCACCCCTTCCTGACCGACCTCGCCGTGCGCCGTGCCATCGCGC
TGGCGATTGACCGCAAGGCTATTGCCGAGCAGCTTTACGGACCGACCGGTGAACCGACCTGCAATATCCTGGTCGTGCCG
GCCAGCGTCAACTCGCCCAACACGAGCTGCGAACGGAATGTCGAAGAGGCGAAGCGGATTCTGGAAGAGGCGGGTTGGGT
GCTGAATGGCTCAGTACGCGAGAAGAATGGGATCAGGCTGATCGTCTCGTTCCAGACCAGCATTAACACGCTGCGCCAGG
GCGAACAGGCGATTATCAAGTCGAACCTGGCCGAAATCGGCATTCAGGTTAACGTGAAGGCTATTGACGCCGCCGTCTTC
TTTGGTGGCGATCCGGGCAACCCCGATACACTGAACAAGTTCTACGCCGACCTGCAAATGTACACCAACGGCCCATCGTC
GACCGACCCGCAGCAGTATTTGCAGGGCTGGATCTGCGCCGAAGTAGCCTCTTCGGCTAACCAGTGGAACGGCAACAACG
ATGGCCGCTACTGCAACCCCGAATACGATGCGCTCTTCGAGGAGTTGAAGGGTGAACTCGACTTGCAGCGCCGGGCAGAG
CTGGCCATTCAGATGAACGATCTGCTGGTCAATGATGTTGCGGTTATTCCGCTGATTAACCGCCGCACGCCCAACGCCAA
GCTGAAGAATCTGGAAGGCCCGACCTTCAATACCTTCGATAGCAGCCTCTGGAATATCGCAAGCTGGCGGCGCGTGCCGT
AA

Upstream 100 bases:

>100_bases
GCGGCAGTGCCGGATCAACGGCAGTGCCTGTGGAATGTGACGGCTGCGGCCAGCGTTGTTCATCAAATACAGGTGTTCGT
TTTAGAGTGAGGAGGTTCCT

Downstream 100 bases:

>100_bases
ACGGTGCCTGCGCGAGGGGTGGCAGGAGCGATCTTGCCGCCTCTCGCACTTCCACGATAGCGAGTTCATCTATCTGCGAC
ATGCTGTACAATGTTGAACG

Product: family 5 extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSHKRLWVWMTLVAVAAMVLAACGTQQPQAQPTTAPAAQATATPQPTAAPQPTAAPAATTAPAPTEAPTAAPQQGGKLTI
LYWQAVTTLNPHLATGTKDFDGATVILEPLARYNEKDELVPFLAAEIPTLENGGVAADGSSVTWKIKPGLKWSDGSDFTI
DDIIFTWQYCADEATACTTKAAFDPIANIEKIDDTTFRIIWKEPNANPYISFVGPLGMILQKKQFENCIGAAASTSAECQ
AANLAPIGTNAWKLKEFRPGDTVIYERNPFYRDAANVFFDEVEIKGGGDATSAARAVCETAEVDFAWNLQIPKAVLEPIL
AAGNCDAIAGGSFGVERIVVNFANPDPALGDKRSEPDQPHPFLTDLAVRRAIALAIDRKAIAEQLYGPTGEPTCNILVVP
ASVNSPNTSCERNVEEAKRILEEAGWVLNGSVREKNGIRLIVSFQTSINTLRQGEQAIIKSNLAEIGIQVNVKAIDAAVF
FGGDPGNPDTLNKFYADLQMYTNGPSSTDPQQYLQGWICAEVASSANQWNGNNDGRYCNPEYDALFEELKGELDLQRRAE
LAIQMNDLLVNDVAVIPLINRRTPNAKLKNLEGPTFNTFDSSLWNIASWRRVP

Sequences:

>Translated_613_residues
MSHKRLWVWMTLVAVAAMVLAACGTQQPQAQPTTAPAAQATATPQPTAAPQPTAAPAATTAPAPTEAPTAAPQQGGKLTI
LYWQAVTTLNPHLATGTKDFDGATVILEPLARYNEKDELVPFLAAEIPTLENGGVAADGSSVTWKIKPGLKWSDGSDFTI
DDIIFTWQYCADEATACTTKAAFDPIANIEKIDDTTFRIIWKEPNANPYISFVGPLGMILQKKQFENCIGAAASTSAECQ
AANLAPIGTNAWKLKEFRPGDTVIYERNPFYRDAANVFFDEVEIKGGGDATSAARAVCETAEVDFAWNLQIPKAVLEPIL
AAGNCDAIAGGSFGVERIVVNFANPDPALGDKRSEPDQPHPFLTDLAVRRAIALAIDRKAIAEQLYGPTGEPTCNILVVP
ASVNSPNTSCERNVEEAKRILEEAGWVLNGSVREKNGIRLIVSFQTSINTLRQGEQAIIKSNLAEIGIQVNVKAIDAAVF
FGGDPGNPDTLNKFYADLQMYTNGPSSTDPQQYLQGWICAEVASSANQWNGNNDGRYCNPEYDALFEELKGELDLQRRAE
LAIQMNDLLVNDVAVIPLINRRTPNAKLKNLEGPTFNTFDSSLWNIASWRRVP
>Mature_612_residues
SHKRLWVWMTLVAVAAMVLAACGTQQPQAQPTTAPAAQATATPQPTAAPQPTAAPAATTAPAPTEAPTAAPQQGGKLTIL
YWQAVTTLNPHLATGTKDFDGATVILEPLARYNEKDELVPFLAAEIPTLENGGVAADGSSVTWKIKPGLKWSDGSDFTID
DIIFTWQYCADEATACTTKAAFDPIANIEKIDDTTFRIIWKEPNANPYISFVGPLGMILQKKQFENCIGAAASTSAECQA
ANLAPIGTNAWKLKEFRPGDTVIYERNPFYRDAANVFFDEVEIKGGGDATSAARAVCETAEVDFAWNLQIPKAVLEPILA
AGNCDAIAGGSFGVERIVVNFANPDPALGDKRSEPDQPHPFLTDLAVRRAIALAIDRKAIAEQLYGPTGEPTCNILVVPA
SVNSPNTSCERNVEEAKRILEEAGWVLNGSVREKNGIRLIVSFQTSINTLRQGEQAIIKSNLAEIGIQVNVKAIDAAVFF
GGDPGNPDTLNKFYADLQMYTNGPSSTDPQQYLQGWICAEVASSANQWNGNNDGRYCNPEYDALFEELKGELDLQRRAEL
AIQMNDLLVNDVAVIPLINRRTPNAKLKNLEGPTFNTFDSSLWNIASWRRVP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=518, Percent_Identity=22.3938223938224, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66293; Mature: 66162

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKRLWVWMTLVAVAAMVLAACGTQQPQAQPTTAPAAQATATPQPTAAPQPTAAPAATT
CCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAPTEAPTAAPQQGGKLTILYWQAVTTLNPHLATGTKDFDGATVILEPLARYNEKDELV
CCCCCCCCCCCCCCCCEEEEEEEEEEHHCCCCCCCCCCCCCCCCCEEHHHHHCCCCHHHH
PFLAAEIPTLENGGVAADGSSVTWKIKPGLKWSDGSDFTIDDIIFTWQYCADEATACTTK
HHHHHCCCCCCCCCEEECCCEEEEEECCCCEECCCCCCCHHHHHEEHHHHCCCHHHHHHH
AAFDPIANIEKIDDTTFRIIWKEPNANPYISFVGPLGMILQKKQFENCIGAAASTSAECQ
HHHCCCCCHHHCCCCEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCC
AANLAPIGTNAWKLKEFRPGDTVIYERNPFYRDAANVFFDEVEIKGGGDATSAARAVCET
CCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHHH
AEVDFAWNLQIPKAVLEPILAAGNCDAIAGGSFGVERIVVNFANPDPALGDKRSEPDQPH
CCCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
PFLTDLAVRRAIALAIDRKAIAEQLYGPTGEPTCNILVVPASVNSPNTSCERNVEEAKRI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHCCHHHHHHH
LEEAGWVLNGSVREKNGIRLIVSFQTSINTLRQGEQAIIKSNLAEIGIQVNVKAIDAAVF
HHHCCCEEECCEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEE
FGGDPGNPDTLNKFYADLQMYTNGPSSTDPQQYLQGWICAEVASSANQWNGNNDGRYCNP
ECCCCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECC
EYDALFEELKGELDLQRRAELAIQMNDLLVNDVAVIPLINRRTPNAKLKNLEGPTFNTFD
HHHHHHHHHCCCCCHHHHHHHEEEECHHEECCEEEEEEECCCCCCCHHCCCCCCCCCCHH
SSLWNIASWRRVP
HHHHHHHHHCCCC
>Mature Secondary Structure 
SHKRLWVWMTLVAVAAMVLAACGTQQPQAQPTTAPAAQATATPQPTAAPQPTAAPAATT
CCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAPTEAPTAAPQQGGKLTILYWQAVTTLNPHLATGTKDFDGATVILEPLARYNEKDELV
CCCCCCCCCCCCCCCCEEEEEEEEEEHHCCCCCCCCCCCCCCCCCEEHHHHHCCCCHHHH
PFLAAEIPTLENGGVAADGSSVTWKIKPGLKWSDGSDFTIDDIIFTWQYCADEATACTTK
HHHHHCCCCCCCCCEEECCCEEEEEECCCCEECCCCCCCHHHHHEEHHHHCCCHHHHHHH
AAFDPIANIEKIDDTTFRIIWKEPNANPYISFVGPLGMILQKKQFENCIGAAASTSAECQ
HHHCCCCCHHHCCCCEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCC
AANLAPIGTNAWKLKEFRPGDTVIYERNPFYRDAANVFFDEVEIKGGGDATSAARAVCET
CCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHHH
AEVDFAWNLQIPKAVLEPILAAGNCDAIAGGSFGVERIVVNFANPDPALGDKRSEPDQPH
CCCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
PFLTDLAVRRAIALAIDRKAIAEQLYGPTGEPTCNILVVPASVNSPNTSCERNVEEAKRI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHCCHHHHHHH
LEEAGWVLNGSVREKNGIRLIVSFQTSINTLRQGEQAIIKSNLAEIGIQVNVKAIDAAVF
HHHCCCEEECCEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEE
FGGDPGNPDTLNKFYADLQMYTNGPSSTDPQQYLQGWICAEVASSANQWNGNNDGRYCNP
ECCCCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECC
EYDALFEELKGELDLQRRAELAIQMNDLLVNDVAVIPLINRRTPNAKLKNLEGPTFNTFD
HHHHHHHHHCCCCCHHHHHHHEEEECHHEECCEEEEEEECCCCCCCHHCCCCCCCCCCHH
SSLWNIASWRRVP
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]