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The map label for this gene is appB [H]

Identifier: 222524135

GI number: 222524135

Start: 1074369

End: 1075328

Strand: Direct

Name: appB [H]

Synonym: Chy400_0851

Alternate gene names: 222524135

Gene position: 1074369-1075328 (Clockwise)

Preceding gene: 222524134

Following gene: 222524136

Centisome position: 20.39

GC content: 50.21

Gene sequence:

>960_bases
ATGTTGCAGTATATTATTCGGCGTATTTTAATTGCTATTCCGACGCTCCTGATTATCAGTTTTGTGATTTTTGCCGTGCT
TGCACTTGCCCCTGGTGATCCACTGGCCCAGTTCGCCCTCAATCCGGCCATTCCCGAATCGACCCGTGAACTGATTCGGA
TTCAGTTTGGCCTGGATCAACCCTGGCCAATTCGCTATGTGCGCTGGTTGACCTCGCTTATGCGGGGGGATTGGGGCTTC
TCGTTTGGTACCCGTGGCCCGGTCATTGATCTGATCTGGCAGCGCCTCCCGCAAACATTAACGGTTGTGGGAACAGCGTA
CCTGATCGCAGTGTTGCTGGCAATCCCGATTGGCATTATTTCAGCCGTAAAGCAGTACTCAATTTTTGATAACGTTGCTA
CCTTTTTTGCATTTATTGGTTTTTCAGTGCCTTCGTTCTTCACCGGTCTGGTGTTGATGCTGATCTTTGCAATTAATCTG
AAATGGTTTCCAATTGTGTACAACACAACATTGCAGGTCGTTGACTGGGAGACCTTCACCCAACAGGTGCGCCAGATGAC
CTTACCGGTGCTGGTGCTGGTTGTGCAACAGACCGCTGCCCTCACCCGCTTCATGCGCTCATCAATGCTTGATAACCTCT
CGCTCGATTATGTGCGCACAGCGCGTGCCAAAGGGTTGAGCGACCGCATGGTTGTGTTACGCCACGTTTTAGTGAACAGC
CTGATCCCCGTGGTCACGTTGATTGCCCTCGGTATTCCCACCATCTTCGCCGGCGCAATTATTACCGAAAATCTCTTCCG
GGTGAATGGTTTGGGTGCGCTGTTGATTACGTCGATCAATAATTCTGATACCCCGGTCGTGATGGCGTTGACGTTCATCT
TTGCCATTTTGACGGTGGTGTTTAATTTGATTGCCGATATTCTCTACGGTGTGCTCGATCCACGAGTACGCTATTCGTAA

Upstream 100 bases:

>100_bases
GAAATATGTCGAAATTATCACGCCGGTGAACCGGCACAGGATATGGGTGAAGCATAACACGGATTTATAGCCGTGCTACA
TTCGACAGGGGCAATGGCGT

Downstream 100 bases:

>100_bases
ACGAGAGGCAACCGGAAGGGGTTAGCGTATGTCTGAAATAGAAATGAACGGCGCCATAGGGATTTCGGCCAGTGCCCGTG
CCCAGCAGATGACCACGCGG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLQYIIRRILIAIPTLLIISFVIFAVLALAPGDPLAQFALNPAIPESTRELIRIQFGLDQPWPIRYVRWLTSLMRGDWGF
SFGTRGPVIDLIWQRLPQTLTVVGTAYLIAVLLAIPIGIISAVKQYSIFDNVATFFAFIGFSVPSFFTGLVLMLIFAINL
KWFPIVYNTTLQVVDWETFTQQVRQMTLPVLVLVVQQTAALTRFMRSSMLDNLSLDYVRTARAKGLSDRMVVLRHVLVNS
LIPVVTLIALGIPTIFAGAIITENLFRVNGLGALLITSINNSDTPVVMALTFIFAILTVVFNLIADILYGVLDPRVRYS

Sequences:

>Translated_319_residues
MLQYIIRRILIAIPTLLIISFVIFAVLALAPGDPLAQFALNPAIPESTRELIRIQFGLDQPWPIRYVRWLTSLMRGDWGF
SFGTRGPVIDLIWQRLPQTLTVVGTAYLIAVLLAIPIGIISAVKQYSIFDNVATFFAFIGFSVPSFFTGLVLMLIFAINL
KWFPIVYNTTLQVVDWETFTQQVRQMTLPVLVLVVQQTAALTRFMRSSMLDNLSLDYVRTARAKGLSDRMVVLRHVLVNS
LIPVVTLIALGIPTIFAGAIITENLFRVNGLGALLITSINNSDTPVVMALTFIFAILTVVFNLIADILYGVLDPRVRYS
>Mature_319_residues
MLQYIIRRILIAIPTLLIISFVIFAVLALAPGDPLAQFALNPAIPESTRELIRIQFGLDQPWPIRYVRWLTSLMRGDWGF
SFGTRGPVIDLIWQRLPQTLTVVGTAYLIAVLLAIPIGIISAVKQYSIFDNVATFFAFIGFSVPSFFTGLVLMLIFAINL
KWFPIVYNTTLQVVDWETFTQQVRQMTLPVLVLVVQQTAALTRFMRSSMLDNLSLDYVRTARAKGLSDRMVVLRHVLVNS
LIPVVTLIALGIPTIFAGAIITENLFRVNGLGALLITSINNSDTPVVMALTFIFAILTVVFNLIADILYGVLDPRVRYS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=339, Percent_Identity=36.5781710914454, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1787053, Length=319, Percent_Identity=37.3040752351097, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789888, Length=323, Percent_Identity=33.7461300309598, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787497, Length=319, Percent_Identity=34.1692789968652, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1788504, Length=362, Percent_Identity=30.3867403314917, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787761, Length=315, Percent_Identity=34.6031746031746, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787550, Length=268, Percent_Identity=30.5970149253731, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35634; Mature: 35634

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQYIIRRILIAIPTLLIISFVIFAVLALAPGDPLAQFALNPAIPESTRELIRIQFGLDQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCC
PWPIRYVRWLTSLMRGDWGFSFGTRGPVIDLIWQRLPQTLTVVGTAYLIAVLLAIPIGII
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVKQYSIFDNVATFFAFIGFSVPSFFTGLVLMLIFAINLKWFPIVYNTTLQVVDWETFT
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECEEEEECHHHHH
QQVRQMTLPVLVLVVQQTAALTRFMRSSMLDNLSLDYVRTARAKGLSDRMVVLRHVLVNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LIPVVTLIALGIPTIFAGAIITENLFRVNGLGALLITSINNSDTPVVMALTFIFAILTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
FNLIADILYGVLDPRVRYS
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLQYIIRRILIAIPTLLIISFVIFAVLALAPGDPLAQFALNPAIPESTRELIRIQFGLDQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCC
PWPIRYVRWLTSLMRGDWGFSFGTRGPVIDLIWQRLPQTLTVVGTAYLIAVLLAIPIGII
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVKQYSIFDNVATFFAFIGFSVPSFFTGLVLMLIFAINLKWFPIVYNTTLQVVDWETFT
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECEEEEECHHHHH
QQVRQMTLPVLVLVVQQTAALTRFMRSSMLDNLSLDYVRTARAKGLSDRMVVLRHVLVNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LIPVVTLIALGIPTIFAGAIITENLFRVNGLGALLITSINNSDTPVVMALTFIFAILTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
FNLIADILYGVLDPRVRYS
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]