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The map label for this gene is purD [H]

Identifier: 222523964

GI number: 222523964

Start: 843864

End: 845108

Strand: Direct

Name: purD [H]

Synonym: Chy400_0679

Alternate gene names: 222523964

Gene position: 843864-845108 (Clockwise)

Preceding gene: 222523962

Following gene: 222523965

Centisome position: 16.02

GC content: 58.07

Gene sequence:

>1245_bases
ATGAACGTATTACTCATTGGTTCCGGTGGACGAGAGCACGCCCTGGCCTGGAAAATTGCCAGCTCGCCTGGATTTACGAA
ACTGTTAACCATTCCCGGTAACCCTGGTACAGGGCGCTACGGACGCAATGTACCATTTCCTCTCACCGATTATCCGGCCC
TGATCGATCTGGCCCAACAGGAAAAGATTGACCTCCTGGTCGTTGGCCCCGATAACCCCCTCGCGGATGGTATTGTCGAT
GCCTTTCAGGCTGCCGGTATTCCGGCATTCGGCCCAACCGCAGCAGCGGCCCGGATCGAGAGCAGCAAATCATTTGCAAA
AGAGATTATGGCCGCTACCGGTGTGCCAACCGCCCAGAGTCATGTCTTTGCCTCGGCTGAAGAGGCAGCAGCATTTGCCC
GTGAAAGCGGTAAAGCGTGGGTGGTGAAGGCTGACGGTCTGGCCCTTGGTAAGGGTGTGATCGTTGCGGAAACGGTTGAC
GAGACCCTGGCCGCGATTGCGAAGTTAAGTGCGATTCCCGCCGGTCACCATCTCTTGCTTGAAGAACGACTATACGGGCG
CGAGGTCTCGGTACAGGCTCTCTGTGATGGCGAGCGGCTCTGGCCGTTGCCACCTGCACGTGACCACAAACGGCTAGAAG
AGGGTGATCGCGGTCCCAACACCGGCGGCATGGGGGTGATTGCACCGGTTGATGAGGTTACACCGGCCTTACTGGACGAA
ATTGTCAATCGTTGTATGCAGCCGGTTGTCAATGAGCTGGCTGCACGGGGCACACCTTTCCGGGGTCTCCTTTACGCCGG
GATCATTCTGACCGCGGATGGCCCAAAGGTGCTTGAGTTTAATGCTCGCTTTGGTGATCCAGAAGCACAGACGGTGTTGC
CGCTGCTGGAAGGCGATTTTCTCGGTGCCCTGTATGCCTGTGCTACCGGCCACATGCAACCCAACCTGCTGCGCCGTCGC
AAGGGATACGCGGTCTGTGTGATCTTGTGTGCTGCTGGTTATCCTGACCGTCCGCGAAAAGGTGATTTGATCCGCGGTGT
GGAAGCGCTTGACGAAGATCAGGTGCTCGTGTTCCACGCCGGCACGACGCTCGGTCCAAATGGTCTGTGTACGGCCGGTG
GACGGGTGCTGGGTGTAACCGGTCTCGGTTCAACCCTGCGCCAGGCGCGTGAACGGGCATACGCTGCTGCCGATCAGATT
CGCTTTGAAGGCAAACATTTTCGCCGTGACATCGGCCAGGAGTAG

Upstream 100 bases:

>100_bases
GATCCTGTGTCACCTGAACACCCTGTCACTCAGTCATCTTAATCATGCTATACTTGTCGCACTGGCTTGTAAGACGCCGA
CCATCGTGATTGATAGGACT

Downstream 100 bases:

>100_bases
GTTGTGCCAGGGATTGCAGTATTGTTGAGCGGCAGCGGGAGCAATTTGCAAGCGCTGCTTGATGCCCAGGCTGCCGGTGA
TCTGGCTGGTGAAGTGGTAC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNVLLIGSGGREHALAWKIASSPGFTKLLTIPGNPGTGRYGRNVPFPLTDYPALIDLAQQEKIDLLVVGPDNPLADGIVD
AFQAAGIPAFGPTAAAARIESSKSFAKEIMAATGVPTAQSHVFASAEEAAAFARESGKAWVVKADGLALGKGVIVAETVD
ETLAAIAKLSAIPAGHHLLLEERLYGREVSVQALCDGERLWPLPPARDHKRLEEGDRGPNTGGMGVIAPVDEVTPALLDE
IVNRCMQPVVNELAARGTPFRGLLYAGIILTADGPKVLEFNARFGDPEAQTVLPLLEGDFLGALYACATGHMQPNLLRRR
KGYAVCVILCAAGYPDRPRKGDLIRGVEALDEDQVLVFHAGTTLGPNGLCTAGGRVLGVTGLGSTLRQARERAYAAADQI
RFEGKHFRRDIGQE

Sequences:

>Translated_414_residues
MNVLLIGSGGREHALAWKIASSPGFTKLLTIPGNPGTGRYGRNVPFPLTDYPALIDLAQQEKIDLLVVGPDNPLADGIVD
AFQAAGIPAFGPTAAAARIESSKSFAKEIMAATGVPTAQSHVFASAEEAAAFARESGKAWVVKADGLALGKGVIVAETVD
ETLAAIAKLSAIPAGHHLLLEERLYGREVSVQALCDGERLWPLPPARDHKRLEEGDRGPNTGGMGVIAPVDEVTPALLDE
IVNRCMQPVVNELAARGTPFRGLLYAGIILTADGPKVLEFNARFGDPEAQTVLPLLEGDFLGALYACATGHMQPNLLRRR
KGYAVCVILCAAGYPDRPRKGDLIRGVEALDEDQVLVFHAGTTLGPNGLCTAGGRVLGVTGLGSTLRQARERAYAAADQI
RFEGKHFRRDIGQE
>Mature_414_residues
MNVLLIGSGGREHALAWKIASSPGFTKLLTIPGNPGTGRYGRNVPFPLTDYPALIDLAQQEKIDLLVVGPDNPLADGIVD
AFQAAGIPAFGPTAAAARIESSKSFAKEIMAATGVPTAQSHVFASAEEAAAFARESGKAWVVKADGLALGKGVIVAETVD
ETLAAIAKLSAIPAGHHLLLEERLYGREVSVQALCDGERLWPLPPARDHKRLEEGDRGPNTGGMGVIAPVDEVTPALLDE
IVNRCMQPVVNELAARGTPFRGLLYAGIILTADGPKVLEFNARFGDPEAQTVLPLLEGDFLGALYACATGHMQPNLLRRR
KGYAVCVILCAAGYPDRPRKGDLIRGVEALDEDQVLVFHAGTTLGPNGLCTAGGRVLGVTGLGSTLRQARERAYAAADQI
RFEGKHFRRDIGQE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=45.6264775413712, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=45.6264775413712, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=45.6264775413712, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=45.6264775413712, Blast_Score=336, Evalue=3e-92,
Organism=Escherichia coli, GI1790438, Length=420, Percent_Identity=44.2857142857143, Blast_Score=332, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=39.9527186761229, Blast_Score=279, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321203, Length=438, Percent_Identity=39.041095890411, Blast_Score=295, Evalue=7e-81,
Organism=Drosophila melanogaster, GI62471619, Length=426, Percent_Identity=42.4882629107981, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24582400, Length=426, Percent_Identity=42.4882629107981, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43816; Mature: 43816

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLLIGSGGREHALAWKIASSPGFTKLLTIPGNPGTGRYGRNVPFPLTDYPALIDLAQQ
CEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHC
EKIDLLVVGPDNPLADGIVDAFQAAGIPAFGPTAAAARIESSKSFAKEIMAATGVPTAQS
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
HVFASAEEAAAFARESGKAWVVKADGLALGKGVIVAETVDETLAAIAKLSAIPAGHHLLL
HHHHCHHHHHHHHHCCCCEEEEEECCEEECCCEEEEHHHHHHHHHHHHHHCCCCCCCEEE
EERLYGREVSVQALCDGERLWPLPPARDHKRLEEGDRGPNTGGMGVIAPVDEVTPALLDE
HHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHCCHHHHHH
IVNRCMQPVVNELAARGTPFRGLLYAGIILTADGPKVLEFNARFGDPEAQTVLPLLEGDF
HHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCHH
LGALYACATGHMQPNLLRRRKGYAVCVILCAAGYPDRPRKGDLIRGVEALDEDQVLVFHA
HHHHHHHHCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEC
GTTLGPNGLCTAGGRVLGVTGLGSTLRQARERAYAAADQIRFEGKHFRRDIGQE
CCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MNVLLIGSGGREHALAWKIASSPGFTKLLTIPGNPGTGRYGRNVPFPLTDYPALIDLAQQ
CEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHC
EKIDLLVVGPDNPLADGIVDAFQAAGIPAFGPTAAAARIESSKSFAKEIMAATGVPTAQS
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
HVFASAEEAAAFARESGKAWVVKADGLALGKGVIVAETVDETLAAIAKLSAIPAGHHLLL
HHHHCHHHHHHHHHCCCCEEEEEECCEEECCCEEEEHHHHHHHHHHHHHHCCCCCCCEEE
EERLYGREVSVQALCDGERLWPLPPARDHKRLEEGDRGPNTGGMGVIAPVDEVTPALLDE
HHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHCCHHHHHH
IVNRCMQPVVNELAARGTPFRGLLYAGIILTADGPKVLEFNARFGDPEAQTVLPLLEGDF
HHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCHH
LGALYACATGHMQPNLLRRRKGYAVCVILCAAGYPDRPRKGDLIRGVEALDEDQVLVFHA
HHHHHHHHCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEC
GTTLGPNGLCTAGGRVLGVTGLGSTLRQARERAYAAADQIRFEGKHFRRDIGQE
CCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]