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The map label for this gene is purN [H]

Identifier: 222523965

GI number: 222523965

Start: 845112

End: 845735

Strand: Direct

Name: purN [H]

Synonym: Chy400_0680

Alternate gene names: 222523965

Gene position: 845112-845735 (Clockwise)

Preceding gene: 222523964

Following gene: 222523968

Centisome position: 16.04

GC content: 60.1

Gene sequence:

>624_bases
GTGCCAGGGATTGCAGTATTGTTGAGCGGCAGCGGGAGCAATTTGCAAGCGCTGCTTGATGCCCAGGCTGCCGGTGATCT
GGCTGGTGAAGTGGTACTGGTCGCAAGTGACCGTGCTCAAGCTTACGGTCTGCAACGGGCATTGCAGGCCGGTGTGGCAG
CGGCGTATATCCCTCTACGGGCAACGCGCGGCCCGCAGCGTCAGCAGTGGGAACAACGGCTGGCCGATATTGTCGCCTGT
TTCGAGCCTGATCTGATCGTCCTCGCCGGCTTTATGCGGGTGCTTTCGGCAGCATTCCTTGAGCGGTTCCCTAACCGGGT
GATTAATCAGCATCCCGCGCTCTTACCCGCGGATGGCGGCGATACCGTGACTACCACCAGTGGGTTGGTTATTCCGGCAT
TGCGTGGTGCGCACGTGGTAGCCGATGCACTACGGCTCGGTTTGCCCGTTACCGGTTGTACCATCCATCGCGTGACACCG
CGGGTGGATGATGGCCCAATTCTGGCCCGCGCTGAGGTTCCGATCCAACCCGATGACACGGTTGAGTCGCTGCACGAACG
GATAAAGGCGGTCGAGCGACGATTGATTGTTGCCACCGTTAACCGCTTACTTGCCGGAGAGTGA

Upstream 100 bases:

>100_bases
TTCAACCCTGCGCCAGGCGCGTGAACGGGCATACGCTGCTGCCGATCAGATTCGCTTTGAAGGCAAACATTTTCGCCGTG
ACATCGGCCAGGAGTAGGTT

Downstream 100 bases:

>100_bases
CCGGATACTACTACCGGTCACTCACCTCATTCGCGACGTAGCAACGCCAGCGTCAACATAATTCCGCCGGTGATCACGCC
ACCAATCCCGATGAACAACA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPGIAVLLSGSGSNLQALLDAQAAGDLAGEVVLVASDRAQAYGLQRALQAGVAAAYIPLRATRGPQRQQWEQRLADIVAC
FEPDLIVLAGFMRVLSAAFLERFPNRVINQHPALLPADGGDTVTTTSGLVIPALRGAHVVADALRLGLPVTGCTIHRVTP
RVDDGPILARAEVPIQPDDTVESLHERIKAVERRLIVATVNRLLAGE

Sequences:

>Translated_207_residues
MPGIAVLLSGSGSNLQALLDAQAAGDLAGEVVLVASDRAQAYGLQRALQAGVAAAYIPLRATRGPQRQQWEQRLADIVAC
FEPDLIVLAGFMRVLSAAFLERFPNRVINQHPALLPADGGDTVTTTSGLVIPALRGAHVVADALRLGLPVTGCTIHRVTP
RVDDGPILARAEVPIQPDDTVESLHERIKAVERRLIVATVNRLLAGE
>Mature_206_residues
PGIAVLLSGSGSNLQALLDAQAAGDLAGEVVLVASDRAQAYGLQRALQAGVAAAYIPLRATRGPQRQQWEQRLADIVACF
EPDLIVLAGFMRVLSAAFLERFPNRVINQHPALLPADGGDTVTTTSGLVIPALRGAHVVADALRLGLPVTGCTIHRVTPR
VDDGPILARAEVPIQPDDTVESLHERIKAVERRLIVATVNRLLAGE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=203, Percent_Identity=33.0049261083744, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI209869995, Length=203, Percent_Identity=33.0049261083744, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI209869993, Length=203, Percent_Identity=33.0049261083744, Blast_Score=123, Evalue=1e-28,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=36.1256544502618, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1787483, Length=204, Percent_Identity=27.9411764705882, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=192, Percent_Identity=35.4166666666667, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=224, Percent_Identity=33.0357142857143, Blast_Score=95, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=41.1458333333333, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21924; Mature: 21792

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIAVLLSGSGSNLQALLDAQAAGDLAGEVVLVASDRAQAYGLQRALQAGVAAAYIPLR
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHC
ATRGPQRQQWEQRLADIVACFEPDLIVLAGFMRVLSAAFLERFPNRVINQHPALLPADGG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
DTVTTTSGLVIPALRGAHVVADALRLGLPVTGCTIHRVTPRVDDGPILARAEVPIQPDDT
CEEEECCCEEEHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCHH
VESLHERIKAVERRLIVATVNRLLAGE
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PGIAVLLSGSGSNLQALLDAQAAGDLAGEVVLVASDRAQAYGLQRALQAGVAAAYIPLR
CCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHHHC
ATRGPQRQQWEQRLADIVACFEPDLIVLAGFMRVLSAAFLERFPNRVINQHPALLPADGG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
DTVTTTSGLVIPALRGAHVVADALRLGLPVTGCTIHRVTPRVDDGPILARAEVPIQPDDT
CEEEECCCEEEHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCHH
VESLHERIKAVERRLIVATVNRLLAGE
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]