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The map label for this gene is rpfG [H]

Identifier: 222523856

GI number: 222523856

Start: 671496

End: 673721

Strand: Direct

Name: rpfG [H]

Synonym: Chy400_0564

Alternate gene names: 222523856

Gene position: 671496-673721 (Clockwise)

Preceding gene: 222523855

Following gene: 222523857

Centisome position: 12.74

GC content: 53.82

Gene sequence:

>2226_bases
ATGTTGGCTTATCCGAACCATCTGCTGCTCATTGAAGATAATCCTTTCGACGCCGATCTGTTCCGACGCACAATGCAGTT
TTACCATCCAGAGATTGCTATCACGCACTTTTCATCATACCGGGCAGCCTACGAACACCTGCAAACATTATCGCCTGCAA
CCACACCTTACGCAGGAGTCGTGCTTGACCTTCATCTACGTGACGGTGATGGGCTTGATCTGCTTGCTCTCATTCGCGCA
CGTAATTTACCACTCGCGGTTGTAATTCTTACCGGTGGTGGTGGCGACCACATGGTCATCAGCGCACTCAAAGCCGGTGC
CGATGATTATGTTGTCAAGCGCGACGATTACCTTGATCGGATCGGTGCGATTGTTGAGCAAGCCTGTCAGCGCTTCCATT
CGCAACAGGCTCGCCGTAGTCAAACCATTCAGGTGTCATTCATCGCTCTGGCGACTACAGATAGCGAAGCGACGATCCAG
CATCTTCAGCGTTCAGCGCCGCATCTCCATTGCACACTCATCCATGGCTGGCGGACGTTTCTGCACCATCTCGATCATCA
GCGGGCAGTCGGCGATGTTATTCTGATCGATTGTGCGCAACCTAATCTGGCAATGCTCGATCTGGTGAAGGAATTGATAC
AGTTTCGTCACCTCGAGCAGCCTGTGCTGGTGCTGGCACAACCGGGTAACGAAGAGTTTCCTTTGCAGACACTACGCCTC
GGCGCAGCAGACTACATTATCAAAAGTGAAGGTTTCCGTAACCATTTGGCGGTGGCGATTGAAAATGCGTATTACCGTGC
CCAGATCCATCAACAACATTATCAATTGCAATTGCAGGCACAGGCGTTGAATGCTGCTGCAAACACGATAGTGATCACGG
ATCGGAACGGTATTATTGAATGGGCTAATCCGGCCTTTACCACGCTCACCGGTTATGAGGTGAGCGAAGTGATTGGTCAT
TCCACCCGGATTCTGCGTTCGGGCAAACACGACAACGAATTTTATAGCGCACTCTGGAATCACATTCTCTCCGGACAGCC
CTGGCGAGGGCGAATTATCAATCGCCGCAAAGATGGTAGCCTTTACCACGAAGAGATGACCATTACACCGGTCACTGATG
AACATGGTGAGATTCGTCGTTTTATCGCGATCAAGCAAGATATTTCCGATCAAGTTGCCCAAGAACAGCGGCGTATCATT
CTGGCTAACATCGGTACAGCCCTGCGACCTGCGCAATTGTGGGTGGAGTTGACGCCGATTTTACTCGATCAGGTGCGCGA
AGTGATGCAGGCCGATAGCATTGCGCTGCTGCGACCACAGGGAGCAACCCTCGCCATCGATCTGGCGTACAACTGTCTGG
GTCATCTCGCCTCGCAACCGACCATACTGGCGGCACTGGCCGAACGGCTGGCACAGAGCGATGGGCCGACAGATGTTACC
GATGTTGTGCAGAGTACGACGAAGATCGTTGTTGGTGTCCCACTCAACGCCGCCAACCGCACCATCGGTGCCCTGCTGAT
CACCCGCGCTACGCCACTTAATTCGTTTGAGGTCGATCTTCTGCGTGATATTGCCGAACTGGCAGCCAATGCACTGCACC
GTACCGATCTCTTCGAGCAACTGCGGGCGGCCAACGCTGAATTACGCGCTGCGTATGATGAGACGATTGAAGGCTGGTCA
CGTGCACTTGATCTGCGTGACCGTGAGACAGAAGGGCATTCCCGGCGGGTAACCGAGTTGACGGTGCGTATTGCCGCCCG
TATGGGTTTCAGTGACGAAGAGCTGCTGCATGTGCGCCGTGGGGCACTCTTGCACGATATTGGCAAAATGGGCATTCCTG
ATGCTATTTTGCTCAAACCCGGCCCACTCGACGAGCAAGAGTGGGCGATTATGCGGACCCATCCAACTCTGGCCGTGGAG
TTACTGCGCCCGATTGCCTTTCTGGCTCCTGCACTCGATATTCCGTGGTGCCACCATGAAAAGTGGGACGGTACCGGCTA
TCCACGTGGCCTGCGTGGCGAAGAAATTCCACTGGCAGCCCGCATCTTTGCGGTGGTTGATGTCTATGATGCACTCACCA
GCGACCGCCCCTATCGAGCAGCATGGCCGCGCGAACGGGCACTGGCCTACATTCGTGAGCAGGCCGGACACCACTTTGAT
CCACACGTGGTTGACGTTTTTGAACAGATCATCACCGAAACAGACAGCAATGCTATAATGAAGTGA

Upstream 100 bases:

>100_bases
GGGCTGGCGATTGTCCGCAAAGCGATTGAACGCATGGGCGGGCGAGTATGGGCCGAAAGCAGTCCCGGTGTCGGTGCTGT
CTTCTATCTGGAGATTCCAC

Downstream 100 bases:

>100_bases
GTGAGTATCGCTGACCACATGGGGAAAGCGATCATTGTGGCTGGTACCAGGTAGGACAGACTACCTGTCATGAGGTGTAT
CTATGATGAACTTTCTCCGG

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 741; Mature: 741

Protein sequence:

>741_residues
MLAYPNHLLLIEDNPFDADLFRRTMQFYHPEIAITHFSSYRAAYEHLQTLSPATTPYAGVVLDLHLRDGDGLDLLALIRA
RNLPLAVVILTGGGGDHMVISALKAGADDYVVKRDDYLDRIGAIVEQACQRFHSQQARRSQTIQVSFIALATTDSEATIQ
HLQRSAPHLHCTLIHGWRTFLHHLDHQRAVGDVILIDCAQPNLAMLDLVKELIQFRHLEQPVLVLAQPGNEEFPLQTLRL
GAADYIIKSEGFRNHLAVAIENAYYRAQIHQQHYQLQLQAQALNAAANTIVITDRNGIIEWANPAFTTLTGYEVSEVIGH
STRILRSGKHDNEFYSALWNHILSGQPWRGRIINRRKDGSLYHEEMTITPVTDEHGEIRRFIAIKQDISDQVAQEQRRII
LANIGTALRPAQLWVELTPILLDQVREVMQADSIALLRPQGATLAIDLAYNCLGHLASQPTILAALAERLAQSDGPTDVT
DVVQSTTKIVVGVPLNAANRTIGALLITRATPLNSFEVDLLRDIAELAANALHRTDLFEQLRAANAELRAAYDETIEGWS
RALDLRDRETEGHSRRVTELTVRIAARMGFSDEELLHVRRGALLHDIGKMGIPDAILLKPGPLDEQEWAIMRTHPTLAVE
LLRPIAFLAPALDIPWCHHEKWDGTGYPRGLRGEEIPLAARIFAVVDVYDALTSDRPYRAAWPRERALAYIREQAGHHFD
PHVVDVFEQIITETDSNAIMK

Sequences:

>Translated_741_residues
MLAYPNHLLLIEDNPFDADLFRRTMQFYHPEIAITHFSSYRAAYEHLQTLSPATTPYAGVVLDLHLRDGDGLDLLALIRA
RNLPLAVVILTGGGGDHMVISALKAGADDYVVKRDDYLDRIGAIVEQACQRFHSQQARRSQTIQVSFIALATTDSEATIQ
HLQRSAPHLHCTLIHGWRTFLHHLDHQRAVGDVILIDCAQPNLAMLDLVKELIQFRHLEQPVLVLAQPGNEEFPLQTLRL
GAADYIIKSEGFRNHLAVAIENAYYRAQIHQQHYQLQLQAQALNAAANTIVITDRNGIIEWANPAFTTLTGYEVSEVIGH
STRILRSGKHDNEFYSALWNHILSGQPWRGRIINRRKDGSLYHEEMTITPVTDEHGEIRRFIAIKQDISDQVAQEQRRII
LANIGTALRPAQLWVELTPILLDQVREVMQADSIALLRPQGATLAIDLAYNCLGHLASQPTILAALAERLAQSDGPTDVT
DVVQSTTKIVVGVPLNAANRTIGALLITRATPLNSFEVDLLRDIAELAANALHRTDLFEQLRAANAELRAAYDETIEGWS
RALDLRDRETEGHSRRVTELTVRIAARMGFSDEELLHVRRGALLHDIGKMGIPDAILLKPGPLDEQEWAIMRTHPTLAVE
LLRPIAFLAPALDIPWCHHEKWDGTGYPRGLRGEEIPLAARIFAVVDVYDALTSDRPYRAAWPRERALAYIREQAGHHFD
PHVVDVFEQIITETDSNAIMK
>Mature_741_residues
MLAYPNHLLLIEDNPFDADLFRRTMQFYHPEIAITHFSSYRAAYEHLQTLSPATTPYAGVVLDLHLRDGDGLDLLALIRA
RNLPLAVVILTGGGGDHMVISALKAGADDYVVKRDDYLDRIGAIVEQACQRFHSQQARRSQTIQVSFIALATTDSEATIQ
HLQRSAPHLHCTLIHGWRTFLHHLDHQRAVGDVILIDCAQPNLAMLDLVKELIQFRHLEQPVLVLAQPGNEEFPLQTLRL
GAADYIIKSEGFRNHLAVAIENAYYRAQIHQQHYQLQLQAQALNAAANTIVITDRNGIIEWANPAFTTLTGYEVSEVIGH
STRILRSGKHDNEFYSALWNHILSGQPWRGRIINRRKDGSLYHEEMTITPVTDEHGEIRRFIAIKQDISDQVAQEQRRII
LANIGTALRPAQLWVELTPILLDQVREVMQADSIALLRPQGATLAIDLAYNCLGHLASQPTILAALAERLAQSDGPTDVT
DVVQSTTKIVVGVPLNAANRTIGALLITRATPLNSFEVDLLRDIAELAANALHRTDLFEQLRAANAELRAAYDETIEGWS
RALDLRDRETEGHSRRVTELTVRIAARMGFSDEELLHVRRGALLHDIGKMGIPDAILLKPGPLDEQEWAIMRTHPTLAVE
LLRPIAFLAPALDIPWCHHEKWDGTGYPRGLRGEEIPLAARIFAVVDVYDALTSDRPYRAAWPRERALAYIREQAGHHFD
PHVVDVFEQIITETDSNAIMK

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Integral Membrane. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 83116; Mature: 83116

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAYPNHLLLIEDNPFDADLFRRTMQFYHPEIAITHFSSYRAAYEHLQTLSPATTPYAGV
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEE
VLDLHLRDGDGLDLLALIRARNLPLAVVILTGGGGDHMVISALKAGADDYVVKRDDYLDR
EEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECHHHHHH
IGAIVEQACQRFHSQQARRSQTIQVSFIALATTDSEATIQHLQRSAPHLHCTLIHGWRTF
HHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHCCCCEEEEEEHHHHHH
LHHLDHQRAVGDVILIDCAQPNLAMLDLVKELIQFRHLEQPVLVLAQPGNEEFPLQTLRL
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHC
GAADYIIKSEGFRNHLAVAIENAYYRAQIHQQHYQLQLQAQALNAAANTIVITDRNGIIE
CCHHHEEECCCCCCCEEEEEECHHHHHHHHHHHHHEEEHHHHHCCCCCEEEEECCCCEEE
WANPAFTTLTGYEVSEVIGHSTRILRSGKHDNEFYSALWNHILSGQPWRGRIINRRKDGS
ECCCCEEEECCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LYHEEMTITPVTDEHGEIRRFIAIKQDISDQVAQEQRRIILANIGTALRPAQLWVELTPI
EEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
LLDQVREVMQADSIALLRPQGATLAIDLAYNCLGHLASQPTILAALAERLAQSDGPTDVT
HHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
DVVQSTTKIVVGVPLNAANRTIGALLITRATPLNSFEVDLLRDIAELAANALHRTDLFEQ
HHHHCCCEEEEEECCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRAANAELRAAYDETIEGWSRALDLRDRETEGHSRRVTELTVRIAARMGFSDEELLHVRR
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
GALLHDIGKMGIPDAILLKPGPLDEQEWAIMRTHPTLAVELLRPIAFLAPALDIPWCHHE
HHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
KWDGTGYPRGLRGEEIPLAARIFAVVDVYDALTSDRPYRAAWPRERALAYIREQAGHHFD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
PHVVDVFEQIITETDSNAIMK
CHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLAYPNHLLLIEDNPFDADLFRRTMQFYHPEIAITHFSSYRAAYEHLQTLSPATTPYAGV
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEE
VLDLHLRDGDGLDLLALIRARNLPLAVVILTGGGGDHMVISALKAGADDYVVKRDDYLDR
EEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECHHHHHH
IGAIVEQACQRFHSQQARRSQTIQVSFIALATTDSEATIQHLQRSAPHLHCTLIHGWRTF
HHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHCCCCEEEEEEHHHHHH
LHHLDHQRAVGDVILIDCAQPNLAMLDLVKELIQFRHLEQPVLVLAQPGNEEFPLQTLRL
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHC
GAADYIIKSEGFRNHLAVAIENAYYRAQIHQQHYQLQLQAQALNAAANTIVITDRNGIIE
CCHHHEEECCCCCCCEEEEEECHHHHHHHHHHHHHEEEHHHHHCCCCCEEEEECCCCEEE
WANPAFTTLTGYEVSEVIGHSTRILRSGKHDNEFYSALWNHILSGQPWRGRIINRRKDGS
ECCCCEEEECCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LYHEEMTITPVTDEHGEIRRFIAIKQDISDQVAQEQRRIILANIGTALRPAQLWVELTPI
EEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
LLDQVREVMQADSIALLRPQGATLAIDLAYNCLGHLASQPTILAALAERLAQSDGPTDVT
HHHHHHHHHCCCCEEEECCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
DVVQSTTKIVVGVPLNAANRTIGALLITRATPLNSFEVDLLRDIAELAANALHRTDLFEQ
HHHHCCCEEEEEECCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRAANAELRAAYDETIEGWSRALDLRDRETEGHSRRVTELTVRIAARMGFSDEELLHVRR
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
GALLHDIGKMGIPDAILLKPGPLDEQEWAIMRTHPTLAVELLRPIAFLAPALDIPWCHHE
HHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
KWDGTGYPRGLRGEEIPLAARIFAVVDVYDALTSDRPYRAAWPRERALAYIREQAGHHFD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
PHVVDVFEQIITETDSNAIMK
CHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]