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The map label for this gene is birA [H]

Identifier: 222523808

GI number: 222523808

Start: 616376

End: 617206

Strand: Direct

Name: birA [H]

Synonym: Chy400_0516

Alternate gene names: 222523808

Gene position: 616376-617206 (Clockwise)

Preceding gene: 222523803

Following gene: 222523809

Centisome position: 11.7

GC content: 55.11

Gene sequence:

>831_bases
ATGATCGATCCTGTTGCAATTCGCGCCGGACTAGAACCAGGCGCGCTGGTTCCGATAACAATCTATTGTTATGATCGAGT
CGGTTCAACGATGGACCTGGCGCGTGAAGCGGTGCAAACACTACCACCTGATGCGTTACCGGTGTTAATCGTTGCCGACG
AACAGACTGCCGGTCGTGGTCGCCTGGGCCGACGCTGGGTAGCTCCGCCCGGCAGTGCTCTGCTCTTCTCACTTGGCCTG
CACCCTCCAGCAGTTGCCACCACCATGCCGACAACTCTAATCTGGCTCGCAACCGTCGCTCTCGTGGAAACTATCGAGCA
AGAGACACCTTTACAAGCCGGCTTGAAGTGGCCCAACGACGTTCTGGTGAAAACGTCAACCGGTTGGGCAAAGACGGCAG
GCATTTTGCTAGAGGGTGGCTGGGACAAGAACAGCCTGGTATGGGCAATTATCGGTTGTGGCATTAACATTAATGCGGCA
CCCGATCCACAGATCACGCGCTATCCAGCGACAGCGCTGGCAATCGCTGCCGAAACTGCTATCGACCGCCTGCGCTTCCT
ACATATGCTGATGCGCCGCTACGATTACTGGCTACGGCGACTGTACAGCGGTGAAAGCGATCAGTTGTGGCAGACATGGC
GTAACCGGCTGCTCACACTGGGACAAACAGTACATATCCATACCGGCAACGATCTGGTGTACGGCACAGCAATTGACGTA
GAACGTAACGGTTCACTCATCGTTCGTGAACCAAGTGGCACACTGCGTCGCATCGAGAGTGGTGACGTTGGTCTGGTCGA
TGACCACTCACGCAATATTAATGAGGTATAA

Upstream 100 bases:

>100_bases
ATTTGATGGATTTTTAGTGATTTTTCATTTTCGATAACCCTGCCCGCCATGGTGTCACGCCAACTCAGGTATGTTATGTA
TGGTGCGATGGAGGTACGGC

Downstream 100 bases:

>100_bases
ACGACCAATTCGAGCCAGTTTTACCGGTCATCAGTGATTTTTCATCCAGACATCATTGCAAGCGGTATTCACGTGCTAAT
AATGATATGGTATAGTAACA

Product: biotin/acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIDPVAIRAGLEPGALVPITIYCYDRVGSTMDLAREAVQTLPPDALPVLIVADEQTAGRGRLGRRWVAPPGSALLFSLGL
HPPAVATTMPTTLIWLATVALVETIEQETPLQAGLKWPNDVLVKTSTGWAKTAGILLEGGWDKNSLVWAIIGCGININAA
PDPQITRYPATALAIAAETAIDRLRFLHMLMRRYDYWLRRLYSGESDQLWQTWRNRLLTLGQTVHIHTGNDLVYGTAIDV
ERNGSLIVREPSGTLRRIESGDVGLVDDHSRNINEV

Sequences:

>Translated_276_residues
MIDPVAIRAGLEPGALVPITIYCYDRVGSTMDLAREAVQTLPPDALPVLIVADEQTAGRGRLGRRWVAPPGSALLFSLGL
HPPAVATTMPTTLIWLATVALVETIEQETPLQAGLKWPNDVLVKTSTGWAKTAGILLEGGWDKNSLVWAIIGCGININAA
PDPQITRYPATALAIAAETAIDRLRFLHMLMRRYDYWLRRLYSGESDQLWQTWRNRLLTLGQTVHIHTGNDLVYGTAIDV
ERNGSLIVREPSGTLRRIESGDVGLVDDHSRNINEV
>Mature_276_residues
MIDPVAIRAGLEPGALVPITIYCYDRVGSTMDLAREAVQTLPPDALPVLIVADEQTAGRGRLGRRWVAPPGSALLFSLGL
HPPAVATTMPTTLIWLATVALVETIEQETPLQAGLKWPNDVLVKTSTGWAKTAGILLEGGWDKNSLVWAIIGCGININAA
PDPQITRYPATALAIAAETAIDRLRFLHMLMRRYDYWLRRLYSGESDQLWQTWRNRLLTLGQTVHIHTGNDLVYGTAIDV
ERNGSLIVREPSGTLRRIESGDVGLVDDHSRNINEV

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 30302; Mature: 30302

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPVAIRAGLEPGALVPITIYCYDRVGSTMDLAREAVQTLPPDALPVLIVADEQTAGRG
CCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
RLGRRWVAPPGSALLFSLGLHPPAVATTMPTTLIWLATVALVETIEQETPLQAGLKWPND
CCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
VLVKTSTGWAKTAGILLEGGWDKNSLVWAIIGCGININAAPDPQITRYPATALAIAAETA
EEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCEECCCCCCCCCEECCHHHHHHHHHHH
IDRLRFLHMLMRRYDYWLRRLYSGESDQLWQTWRNRLLTLGQTVHIHTGNDLVYGTAIDV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEE
ERNGSLIVREPSGTLRRIESGDVGLVDDHSRNINEV
ECCCCEEEECCCCCEEEECCCCCCEECCCCCCCCCC
>Mature Secondary Structure
MIDPVAIRAGLEPGALVPITIYCYDRVGSTMDLAREAVQTLPPDALPVLIVADEQTAGRG
CCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
RLGRRWVAPPGSALLFSLGLHPPAVATTMPTTLIWLATVALVETIEQETPLQAGLKWPND
CCCCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
VLVKTSTGWAKTAGILLEGGWDKNSLVWAIIGCGININAAPDPQITRYPATALAIAAETA
EEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCEECCCCCCCCCEECCHHHHHHHHHHH
IDRLRFLHMLMRRYDYWLRRLYSGESDQLWQTWRNRLLTLGQTVHIHTGNDLVYGTAIDV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEE
ERNGSLIVREPSGTLRRIESGDVGLVDDHSRNINEV
ECCCCEEEECCCCCEEEECCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]