Click here to switch to the map view.

The map label for this gene is spsK [H]

Identifier: 222523729

GI number: 222523729

Start: 514371

End: 515225

Strand: Reverse

Name: spsK [H]

Synonym: Chy400_0435

Alternate gene names: 222523729

Gene position: 515225-514371 (Counterclockwise)

Preceding gene: 222523730

Following gene: 222523728

Centisome position: 9.78

GC content: 56.02

Gene sequence:

>855_bases
GTGCGGATTGCTATTACCGGAGCTAATGGGCAGTTAGGACGGGCACTGATCGCTACATTGGCCGATCAGCATACGCTGGT
GCCGCTAGGGCACGATCAGTTGGAGTTGACCGATCCGGTAACTGTTGAGCAGATTGCGGCGACTGATGCTGATGTGGTAA
TTCACGCCGCAGCGTATACCAATGTCGATGGATGTGCACGTGATCCGGGATTAGCGTATCGGGTGAATGGCCTGGGCACG
CGCTATGTGGCACTCGGTTGTCGGCGAATCGATGCGGCGCTGGTCTACATCAGTACCAACGAGGTGTTTGCCGGTGATGC
CCGCCGGCCCTACTTTGAAGACGATCCCCCTCGTGCGATTAATCCGTATGGGCAGAGTAAACTGGCCGGTGAACAGGCGG
TTCGTTCGCTTGTCGCCCGGCATTTCATTGTGCGGGTAGCCTGGCTGTTTGGCGGTGAACGTAATTTTGTTCGTACTGTC
TTACGGCTGGCAGCCAATCCGCCCGCTCATGGTTTACGTATGGTAGCCGACGAGATCGGTAGTCCGACCTACACCTTTGA
TGTTGCGGCGGGGCTGGCCCGGTTGATCACAACCGACTACTACGGCACCTATCATTTTGTGAACGATGGTATCTGCTCGC
GGTATGAATTTGCGGCGGAGATTCTTCGTCGTGTTGGGTTGAATATTCCGCTCCAGCCGATCCGTCTGTGTGATTTTCAG
CGTGACAGTACGCCACCTCCATACACGCCCCTGGCTAATCTTGCTGGTGCCAGCCTGGGCATCACCTTTCGTCCATGGCA
GGATGCACTCGCCGACTATTTGGGACGCCTGCCAAATGAAGTGTTGCCGACATGA

Upstream 100 bases:

>100_bases
GGCTCTACGTGCTCCCTTATAGCAGTCGTATCGGTTGACGCCGGTGTGCTCTTCTGCTACTCTGTGTAGCCGGTAATTGA
TTAAGTCGAGGAGACGCCTT

Downstream 100 bases:

>100_bases
TTGACGTTGTTGTGCCTAATTACAACGGTAGTGCACTGTTGCCGACCTGTCTTGACTCGTTACGTGCCCAGACCCGGCGT
GATTTTACGGTGACGGTGGT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRIAITGANGQLGRALIATLADQHTLVPLGHDQLELTDPVTVEQIAATDADVVIHAAAYTNVDGCARDPGLAYRVNGLGT
RYVALGCRRIDAALVYISTNEVFAGDARRPYFEDDPPRAINPYGQSKLAGEQAVRSLVARHFIVRVAWLFGGERNFVRTV
LRLAANPPAHGLRMVADEIGSPTYTFDVAAGLARLITTDYYGTYHFVNDGICSRYEFAAEILRRVGLNIPLQPIRLCDFQ
RDSTPPPYTPLANLAGASLGITFRPWQDALADYLGRLPNEVLPT

Sequences:

>Translated_284_residues
MRIAITGANGQLGRALIATLADQHTLVPLGHDQLELTDPVTVEQIAATDADVVIHAAAYTNVDGCARDPGLAYRVNGLGT
RYVALGCRRIDAALVYISTNEVFAGDARRPYFEDDPPRAINPYGQSKLAGEQAVRSLVARHFIVRVAWLFGGERNFVRTV
LRLAANPPAHGLRMVADEIGSPTYTFDVAAGLARLITTDYYGTYHFVNDGICSRYEFAAEILRRVGLNIPLQPIRLCDFQ
RDSTPPPYTPLANLAGASLGITFRPWQDALADYLGRLPNEVLPT
>Mature_284_residues
MRIAITGANGQLGRALIATLADQHTLVPLGHDQLELTDPVTVEQIAATDADVVIHAAAYTNVDGCARDPGLAYRVNGLGT
RYVALGCRRIDAALVYISTNEVFAGDARRPYFEDDPPRAINPYGQSKLAGEQAVRSLVARHFIVRVAWLFGGERNFVRTV
LRLAANPPAHGLRMVADEIGSPTYTFDVAAGLARLITTDYYGTYHFVNDGICSRYEFAAEILRRVGLNIPLQPIRLCDFQ
RDSTPPPYTPLANLAGASLGITFRPWQDALADYLGRLPNEVLPT

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=252, Percent_Identity=27.3809523809524, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI11034825, Length=252, Percent_Identity=27.3809523809524, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788352, Length=267, Percent_Identity=31.4606741573034, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31006; Mature: 31006

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAITGANGQLGRALIATLADQHTLVPLGHDQLELTDPVTVEQIAATDADVVIHAAAYT
CEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCHHHHHCCCCCEEEEEEHHC
NVDGCARDPGLAYRVNGLGTRYVALGCRRIDAALVYISTNEVFAGDARRPYFEDDPPRAI
CCCCCCCCCCCEEEECCCCHHHHHHHHEEEEEEEEEEECCCEEECCCCCCCCCCCCCCCC
NPYGQSKLAGEQAVRSLVARHFIVRVAWLFGGERNFVRTVLRLAANPPAHGLRMVADEIG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHC
SPTYTFDVAAGLARLITTDYYGTYHFVNDGICSRYEFAAEILRRVGLNIPLQPIRLCDFQ
CCCEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
RDSTPPPYTPLANLAGASLGITFRPWQDALADYLGRLPNEVLPT
CCCCCCCCCHHHHHCCCCCCEEECCHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MRIAITGANGQLGRALIATLADQHTLVPLGHDQLELTDPVTVEQIAATDADVVIHAAAYT
CEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCHHHHHCCCCCEEEEEEHHC
NVDGCARDPGLAYRVNGLGTRYVALGCRRIDAALVYISTNEVFAGDARRPYFEDDPPRAI
CCCCCCCCCCCEEEECCCCHHHHHHHHEEEEEEEEEEECCCEEECCCCCCCCCCCCCCCC
NPYGQSKLAGEQAVRSLVARHFIVRVAWLFGGERNFVRTVLRLAANPPAHGLRMVADEIG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHC
SPTYTFDVAAGLARLITTDYYGTYHFVNDGICSRYEFAAEILRRVGLNIPLQPIRLCDFQ
CCCEEHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
RDSTPPPYTPLANLAGASLGITFRPWQDALADYLGRLPNEVLPT
CCCCCCCCCHHHHHCCCCCCEEECCHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]