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The map label for this gene is pleD [H]

Identifier: 222523730

GI number: 222523730

Start: 515467

End: 516948

Strand: Reverse

Name: pleD [H]

Synonym: Chy400_0436

Alternate gene names: 222523730

Gene position: 516948-515467 (Counterclockwise)

Preceding gene: 222523731

Following gene: 222523729

Centisome position: 9.81

GC content: 51.75

Gene sequence:

>1482_bases
ATGGAGCAAAATACCATCTTGATCGTTGATGACGATGAGCTGATGCGTTCATTGCTCATCGAAATAATAACCCCCTTCAA
TCTCCGCCTGATCACTGCGCAGCGCGGTGATCAGGCGCTGCAACTTGCACAGCAGCATCTGCCCGATCTTATCCTGCTTG
ATGTTATGCTGCCGGATATGAATGGCTTCGAGGTGTGCCAGCGCTTACGTGACGATCCGGCAACAGCTCAGATACCGATT
GTCATGATTACCGCGCTGAATGATCGTGAAGCGAAAATTCATGGCTTCGAGGCCGGTGCTGATGAATTTATCACCAAGCC
GTTCGATCCGGGAGAGTTACAGGCCCGGATTAACACGGTCTTACGCCTCAATCGCTATCGGCGTCTGCTGCAGGAGCAGG
CCAAAGTGAACGTCGAACGGGCGCGTTTCGAGTGGGTGATCGAGACATCCGATAACGCTTATGTCATTATTGATCACGAT
GATCAGATCCGTTACGCCAACCGCCGTGCACGCACGTATCTTGGTCTAACCGCCAATGAACAGCCGGTCAGGCCATTCCG
TGAACTGGTTACGCAGCGCTACCGTCTGGTTCCCCCTGAAGCATGGGAGCAATGGCCGATAGGGGCGCCTGAACTACTCT
ATCTCGTGCATCCTGAAACATCACGCAGCCCGGAACAGTGGCTTCAGGTTGAAGCTGCGCGTATTCATCCCTCTGATGAT
CAAATTGTGGTCACTTTGTGCGATGTTACACAGCAGATTACGTTACAACGCGATATGTGGACGTTCCACACGGCAATTTC
ACATAAATTGCGCACCCCGCTCGTTAGCTATTTGGGTGGACTAAACCTTTTGTACGACAATATTGAGCATATGGATCGTA
CCATGGCGCGTAATATTGCGGCGATTGCGCTTAGTGCTGCTAAACGCTTACAGCAGGTGATTGATGATATTTTTCTGTAC
GTTCGCAGTCCGGGTGATATACATTATGATAACTGTACCGTGTTTGATTTACCGGCCATGGTGAGCACGCTCAATCAGGA
ACTTGAGATTCCAGCGATTAGTTTGAGTATCGATCCGGTGCTGGATGCACGTCGGCTGCGTATATCGCGGCGCAGCCTGG
AGATGGTGCTGCGTGAGCTATGCGAAAATGCACGGAAGTTTCATCCCCAACAGCGGCCAATGGTGACTATTCGGGTTGAT
GCCGATCTGGCGCAAAATCAGGTGCGCCTGGTAGTGTGTGACGATGGTGTGCATTTACCCCCGGCTCACTTACAAAAAAT
CAGGCAGCCGTATTATCAGGCTGAGCGTAACTTTACCGGCCAGGTTGAGGGTATGGGTTTGGGGCTGGCACAGGTGGCAC
GCATTATTCTGGCGGTTGGTGGGAGCTACCAGATGCGGAATCGTTCGGATCGCCCTGGGATTTGTGTCGAATTGCGCATT
CCTTTTGTCGAGGAAGACTCTTCTGATCGGGTTATCGGTTAG

Upstream 100 bases:

>100_bases
GATAGGATGCCCCAATTGTTGCGAACCTATCCGTAATCTCGTTGCCAAAGTGAACAATTTTGTCACATCATTCGTAATAG
CTCCATGGTAGGATATTATC

Downstream 100 bases:

>100_bases
CAGGTTCAGGTTAGATTAGCCTGGATGCACATACGAACATCGGCAGAGGCTTCTTCGTCAACGGCTGGAACCGCTCATTG
TTGGTGGCTGTAACCCGGCA

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MEQNTILIVDDDELMRSLLIEIITPFNLRLITAQRGDQALQLAQQHLPDLILLDVMLPDMNGFEVCQRLRDDPATAQIPI
VMITALNDREAKIHGFEAGADEFITKPFDPGELQARINTVLRLNRYRRLLQEQAKVNVERARFEWVIETSDNAYVIIDHD
DQIRYANRRARTYLGLTANEQPVRPFRELVTQRYRLVPPEAWEQWPIGAPELLYLVHPETSRSPEQWLQVEAARIHPSDD
QIVVTLCDVTQQITLQRDMWTFHTAISHKLRTPLVSYLGGLNLLYDNIEHMDRTMARNIAAIALSAAKRLQQVIDDIFLY
VRSPGDIHYDNCTVFDLPAMVSTLNQELEIPAISLSIDPVLDARRLRISRRSLEMVLRELCENARKFHPQQRPMVTIRVD
ADLAQNQVRLVVCDDGVHLPPAHLQKIRQPYYQAERNFTGQVEGMGLGLAQVARIILAVGGSYQMRNRSDRPGICVELRI
PFVEEDSSDRVIG

Sequences:

>Translated_493_residues
MEQNTILIVDDDELMRSLLIEIITPFNLRLITAQRGDQALQLAQQHLPDLILLDVMLPDMNGFEVCQRLRDDPATAQIPI
VMITALNDREAKIHGFEAGADEFITKPFDPGELQARINTVLRLNRYRRLLQEQAKVNVERARFEWVIETSDNAYVIIDHD
DQIRYANRRARTYLGLTANEQPVRPFRELVTQRYRLVPPEAWEQWPIGAPELLYLVHPETSRSPEQWLQVEAARIHPSDD
QIVVTLCDVTQQITLQRDMWTFHTAISHKLRTPLVSYLGGLNLLYDNIEHMDRTMARNIAAIALSAAKRLQQVIDDIFLY
VRSPGDIHYDNCTVFDLPAMVSTLNQELEIPAISLSIDPVLDARRLRISRRSLEMVLRELCENARKFHPQQRPMVTIRVD
ADLAQNQVRLVVCDDGVHLPPAHLQKIRQPYYQAERNFTGQVEGMGLGLAQVARIILAVGGSYQMRNRSDRPGICVELRI
PFVEEDSSDRVIG
>Mature_493_residues
MEQNTILIVDDDELMRSLLIEIITPFNLRLITAQRGDQALQLAQQHLPDLILLDVMLPDMNGFEVCQRLRDDPATAQIPI
VMITALNDREAKIHGFEAGADEFITKPFDPGELQARINTVLRLNRYRRLLQEQAKVNVERARFEWVIETSDNAYVIIDHD
DQIRYANRRARTYLGLTANEQPVRPFRELVTQRYRLVPPEAWEQWPIGAPELLYLVHPETSRSPEQWLQVEAARIHPSDD
QIVVTLCDVTQQITLQRDMWTFHTAISHKLRTPLVSYLGGLNLLYDNIEHMDRTMARNIAAIALSAAKRLQQVIDDIFLY
VRSPGDIHYDNCTVFDLPAMVSTLNQELEIPAISLSIDPVLDARRLRISRRSLEMVLRELCENARKFHPQQRPMVTIRVD
ADLAQNQVRLVVCDDGVHLPPAHLQKIRQPYYQAERNFTGQVEGMGLGLAQVARIILAVGGSYQMRNRSDRPGICVELRI
PFVEEDSSDRVIG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=149, Percent_Identity=37.5838926174497, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=39.3162393162393, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=39.6694214876033, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=34.9593495934959, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=38.4615384615385, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=36.1344537815126, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693140, Length=243, Percent_Identity=27.1604938271605, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI2367329, Length=123, Percent_Identity=39.0243902439024, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787375, Length=119, Percent_Identity=31.0924369747899, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790863, Length=193, Percent_Identity=26.4248704663212, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=29.9145299145299, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=125, Percent_Identity=28.8, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787229, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790552, Length=120, Percent_Identity=35, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=109, Percent_Identity=26.605504587156, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 56579; Mature: 56579

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQNTILIVDDDELMRSLLIEIITPFNLRLITAQRGDQALQLAQQHLPDLILLDVMLPDM
CCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEHHHCCCC
NGFEVCQRLRDDPATAQIPIVMITALNDREAKIHGFEAGADEFITKPFDPGELQARINTV
CHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEECCCCCCHHHHCCCCCCHHHHHHHHHH
LRLNRYRRLLQEQAKVNVERARFEWVIETSDNAYVIIDHDDQIRYANRRARTYLGLTANE
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEEEECCCCCHHHCCHHEEEEEECCCC
QPVRPFRELVTQRYRLVPPEAWEQWPIGAPELLYLVHPETSRSPEQWLQVEAARIHPSDD
CCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCCCCCHHHHHEEHHEEECCCCC
QIVVTLCDVTQQITLQRDMWTFHTAISHKLRTPLVSYLGGLNLLYDNIEHMDRTMARNIA
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AIALSAAKRLQQVIDDIFLYVRSPGDIHYDNCTVFDLPAMVSTLNQELEIPAISLSIDPV
HHHHHHHHHHHHHHHHHHEEEECCCCEEECCEEEEEHHHHHHHHCCCCCCCEEEEECCCC
LDARRLRISRRSLEMVLRELCENARKFHPQQRPMVTIRVDADLAQNQVRLVVCDDGVHLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCC
PAHLQKIRQPYYQAERNFTGQVEGMGLGLAQVARIILAVGGSYQMRNRSDRPGICVELRI
HHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEE
PFVEEDSSDRVIG
CEEECCCCCCCCC
>Mature Secondary Structure
MEQNTILIVDDDELMRSLLIEIITPFNLRLITAQRGDQALQLAQQHLPDLILLDVMLPDM
CCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEHHHCCCC
NGFEVCQRLRDDPATAQIPIVMITALNDREAKIHGFEAGADEFITKPFDPGELQARINTV
CHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEECCCCCCHHHHCCCCCCHHHHHHHHHH
LRLNRYRRLLQEQAKVNVERARFEWVIETSDNAYVIIDHDDQIRYANRRARTYLGLTANE
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEEEECCCCCHHHCCHHEEEEEECCCC
QPVRPFRELVTQRYRLVPPEAWEQWPIGAPELLYLVHPETSRSPEQWLQVEAARIHPSDD
CCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCCCCCHHHHHEEHHEEECCCCC
QIVVTLCDVTQQITLQRDMWTFHTAISHKLRTPLVSYLGGLNLLYDNIEHMDRTMARNIA
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AIALSAAKRLQQVIDDIFLYVRSPGDIHYDNCTVFDLPAMVSTLNQELEIPAISLSIDPV
HHHHHHHHHHHHHHHHHHEEEECCCCEEECCEEEEEHHHHHHHHCCCCCCCEEEEECCCC
LDARRLRISRRSLEMVLRELCENARKFHPQQRPMVTIRVDADLAQNQVRLVVCDDGVHLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCC
PAHLQKIRQPYYQAERNFTGQVEGMGLGLAQVARIILAVGGSYQMRNRSDRPGICVELRI
HHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEE
PFVEEDSSDRVIG
CEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA