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The map label for this gene is hisH

Identifier: 222523664

GI number: 222523664

Start: 437315

End: 437953

Strand: Direct

Name: hisH

Synonym: Chy400_0370

Alternate gene names: 222523664

Gene position: 437315-437953 (Clockwise)

Preceding gene: 222523663

Following gene: 222523665

Centisome position: 8.3

GC content: 57.28

Gene sequence:

>639_bases
ATGATAGCAGTGATTAACTACGGTGCCGGCAACCTGCCCAATGTGGTACGGGCACTACAACGGGTCGGCGCTACACTCAC
CGTAACCGATAACCCAGAGGTGATCCGTTCTGCGCAAGCGGTCGTTCTGCCCGGTGTTGGCGCGACCGCTGATACCATGG
CCAGTCTGCGCCACCTGGGGATTGCAGAGGTCTTACCGGCAGTTATCGCTGCCAGTACACCGTTTCTGGGTATTTGCGTC
GGGATGCAGGTCTTGCTGAGTGAAAGTGAAGAGTTTGGTCTCCACTCGTGTCTCGACATCATCCCCGGCACAGTACGCCG
TCTTCCCGAACACGCCGGCAAGATACCGCAGATCGGCTGGAATCAGCTTCAGATCAGCCCGACATTCCGCAACCATCCAC
TCTTTGCCGATATTCCTGATGGAGCCGATGTCTATTTCGTGCATTCGTATTACTGTGCGGTAGCTGATGAGGCGATCATC
GCGGCCCGCACCGACTACGGCATCCCCTTTCCGAGCGTCATTATTCGCGACCACTTGGCGGCAGTACAGTTCCATCCCGA
AAAGAGTGGCGACTATGGCTTGCGCCTGCTGGCCAATTTCGTGCGCTGGAGCGAGGCCGTCCAGCCAAAAGGAGTGTGA

Upstream 100 bases:

>100_bases
TGGGCAAGATGAATGCACCCGAACCGGCCATCGAACACATCATCACCAGTCTCTACACCTACCATCCTCTGCTGATGCAG
ACAACATATCAGGGAGAAGC

Downstream 100 bases:

>100_bases
CAATGGAGATTATTCCGGCAATTGATATAAAAGATGGTCGCTGCGTCCGCTTGTATCAAGGCGATTTTGCCCAAATGACG
GTCTACGCCGACGATCCGGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIAVINYGAGNLPNVVRALQRVGATLTVTDNPEVIRSAQAVVLPGVGATADTMASLRHLGIAEVLPAVIAASTPFLGICV
GMQVLLSESEEFGLHSCLDIIPGTVRRLPEHAGKIPQIGWNQLQISPTFRNHPLFADIPDGADVYFVHSYYCAVADEAII
AARTDYGIPFPSVIIRDHLAAVQFHPEKSGDYGLRLLANFVRWSEAVQPKGV

Sequences:

>Translated_212_residues
MIAVINYGAGNLPNVVRALQRVGATLTVTDNPEVIRSAQAVVLPGVGATADTMASLRHLGIAEVLPAVIAASTPFLGICV
GMQVLLSESEEFGLHSCLDIIPGTVRRLPEHAGKIPQIGWNQLQISPTFRNHPLFADIPDGADVYFVHSYYCAVADEAII
AARTDYGIPFPSVIIRDHLAAVQFHPEKSGDYGLRLLANFVRWSEAVQPKGV
>Mature_212_residues
MIAVINYGAGNLPNVVRALQRVGATLTVTDNPEVIRSAQAVVLPGVGATADTMASLRHLGIAEVLPAVIAASTPFLGICV
GMQVLLSESEEFGLHSCLDIIPGTVRRLPEHAGKIPQIGWNQLQISPTFRNHPLFADIPDGADVYFVHSYYCAVADEAII
AARTDYGIPFPSVIIRDHLAAVQFHPEKSGDYGLRLLANFVRWSEAVQPKGV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=201, Percent_Identity=37.3134328358209, Blast_Score=124, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=28.6384976525822, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CHLAA (A9WD25)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001633983.1
- ProteinModelPortal:   A9WD25
- GeneID:   5827956
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0343
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22775; Mature: 22775

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 185-185 ACT_SITE 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVINYGAGNLPNVVRALQRVGATLTVTDNPEVIRSAQAVVLPGVGATADTMASLRHLG
CEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHCCCEEEECCCCCCHHHHHHHHHCC
IAEVLPAVIAASTPFLGICVGMQVLLSESEEFGLHSCLDIIPGTVRRLPEHAGKIPQIGW
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCHHHHHHHCCHHHHHHHHHCCCCCCCCC
NQLQISPTFRNHPLFADIPDGADVYFVHSYYCAVADEAIIAARTDYGIPFPSVIIRDHLA
CEEEECCCCCCCCEEEECCCCCCEEEEEHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
AVQFHPEKSGDYGLRLLANFVRWSEAVQPKGV
HEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIAVINYGAGNLPNVVRALQRVGATLTVTDNPEVIRSAQAVVLPGVGATADTMASLRHLG
CEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHCCCEEEECCCCCCHHHHHHHHHCC
IAEVLPAVIAASTPFLGICVGMQVLLSESEEFGLHSCLDIIPGTVRRLPEHAGKIPQIGW
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCHHHHHHHCCHHHHHHHHHCCCCCCCCC
NQLQISPTFRNHPLFADIPDGADVYFVHSYYCAVADEAIIAARTDYGIPFPSVIIRDHLA
CEEEECCCCCCCCEEEECCCCCCEEEEEHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
AVQFHPEKSGDYGLRLLANFVRWSEAVQPKGV
HEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA