Click here to switch to the map view.

The map label for this gene is hpaIM [H]

Identifier: 222523640

GI number: 222523640

Start: 410454

End: 411656

Strand: Direct

Name: hpaIM [H]

Synonym: Chy400_0346

Alternate gene names: 222523640

Gene position: 410454-411656 (Clockwise)

Preceding gene: 222523639

Following gene: 222523646

Centisome position: 7.79

GC content: 41.73

Gene sequence:

>1203_bases
ATGACAGTGCTTTGTGGAGATTGTAGAATTGTTTTACAAAGTATAAATGACAGCAGCATTGATCTTGTGTATATGGATCC
GCCATTTTTTACACAAAAAACCCATGCTCTTGTCAATCGCGATGGTAATGCATCGTATTCATTTGAAGACACCTGGGAAT
CTATCGAAACATATCTTGCCTTTATGAGAGAGGTTTTGATACAGTGCAAACGTGTGCTTAAGGATACTGGTAGCATCTTT
GTTCACTGTGATCGGTCAGCCTCCCATCATCTTCGTGTACTTCTTGATCAGATATTTGGCCCAGAACACTTTCAAAGTGA
GATTATCTGGACATATCGGCGATGGTCAAACTCAAAAAAAGGTCTTCTCCCGGCTCATCAGACAATATATTTTTACAGTA
AGACGGAGAACTTTAAGTTTAATGTTCTTTTTACTGACTATTCACCAACCACAAACATTGATCAGATTTTACAGGCAAGA
GCGCGCAATGAAAAAGGAAAGTCGGTATATCGTCGCGATCAACACGGTAACATTGTCGCAGGTAAGGAGAAAAAAGGAGT
TCCGCTTTCTGATGTCTGGTATATCCCGTTCCTAAACCCAAAAGCAAAGGAAAGAGTTGGCTATCCTACGCAGAAGCCTG
TTTTGTTGTTAGAGCGAATCATCAAAATCGCAACCGACAGTGGGGACAGCGTACTAGACCCTTTCTGCGGTAGTGGAACA
ACACTCGTTGCTGCAAAGTTACTCGGACGAACATATATTGGCATTGATATATCGCGAGAAGCGGTTAGCCTGTCGGAAGA
ACGATTACGCCAGCCTATCAAAAGCGAGTCACAGTTGCTCGTCGTCGGCGAGGAAGGGTTTCGGATGAAGTCTAACTATG
AACGAAACCTGTTAAAAGCACTTGATGCCGTTCCTGTCGAAAGGAACAGCGGCATAGATGGATTTTTGTTTATAGATGAG
CAGCCCATACCTGTACGAATTCAAAAAGATGATGAAGATATTGCAACTGCCAGGCAAAAGTTGAAAGCTGCGAGTAGAGC
AAAGCAATGCTCATCGATGATTTTAATTAGAACAAAGACTGAGAACAATCTATGTCTTTTTGAAACAGAAGATGATGATG
TCCTGGTTATAGATGCTTACGATCTTATCATCCGTTCCTGGCTTCATAAACGGAAGTATCGCAAGAAAACAACAGTTTCA
TAG

Upstream 100 bases:

>100_bases
CTGGAGATGATGCGTGGGAATATGTAAGAACCAGAACAGGCATTGATTTAAAGGTGATTCTGGTCAATATTGCAAAAGAT
TTGCAATGAAAGGAGTGGAT

Downstream 100 bases:

>100_bases
AGTATATTTCTGCCGAAACCGCATTTCTGTCAAAAGCATTGACCGGTAAATTATCAATCACGGCTTGTGGAAGACTGCTG
CGACAAGCGCCTGGAATTTA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTVLCGDCRIVLQSINDSSIDLVYMDPPFFTQKTHALVNRDGNASYSFEDTWESIETYLAFMREVLIQCKRVLKDTGSIF
VHCDRSASHHLRVLLDQIFGPEHFQSEIIWTYRRWSNSKKGLLPAHQTIYFYSKTENFKFNVLFTDYSPTTNIDQILQAR
ARNEKGKSVYRRDQHGNIVAGKEKKGVPLSDVWYIPFLNPKAKERVGYPTQKPVLLLERIIKIATDSGDSVLDPFCGSGT
TLVAAKLLGRTYIGIDISREAVSLSEERLRQPIKSESQLLVVGEEGFRMKSNYERNLLKALDAVPVERNSGIDGFLFIDE
QPIPVRIQKDDEDIATARQKLKAASRAKQCSSMILIRTKTENNLCLFETEDDDVLVIDAYDLIIRSWLHKRKYRKKTTVS

Sequences:

>Translated_400_residues
MTVLCGDCRIVLQSINDSSIDLVYMDPPFFTQKTHALVNRDGNASYSFEDTWESIETYLAFMREVLIQCKRVLKDTGSIF
VHCDRSASHHLRVLLDQIFGPEHFQSEIIWTYRRWSNSKKGLLPAHQTIYFYSKTENFKFNVLFTDYSPTTNIDQILQAR
ARNEKGKSVYRRDQHGNIVAGKEKKGVPLSDVWYIPFLNPKAKERVGYPTQKPVLLLERIIKIATDSGDSVLDPFCGSGT
TLVAAKLLGRTYIGIDISREAVSLSEERLRQPIKSESQLLVVGEEGFRMKSNYERNLLKALDAVPVERNSGIDGFLFIDE
QPIPVRIQKDDEDIATARQKLKAASRAKQCSSMILIRTKTENNLCLFETEDDDVLVIDAYDLIIRSWLHKRKYRKKTTVS
>Mature_399_residues
TVLCGDCRIVLQSINDSSIDLVYMDPPFFTQKTHALVNRDGNASYSFEDTWESIETYLAFMREVLIQCKRVLKDTGSIFV
HCDRSASHHLRVLLDQIFGPEHFQSEIIWTYRRWSNSKKGLLPAHQTIYFYSKTENFKFNVLFTDYSPTTNIDQILQARA
RNEKGKSVYRRDQHGNIVAGKEKKGVPLSDVWYIPFLNPKAKERVGYPTQKPVLLLERIIKIATDSGDSVLDPFCGSGTT
LVAAKLLGRTYIGIDISREAVSLSEERLRQPIKSESQLLVVGEEGFRMKSNYERNLLKALDAVPVERNSGIDGFLFIDEQ
PIPVRIQKDDEDIATARQKLKAASRAKQCSSMILIRTKTENNLCLFETEDDDVLVIDAYDLIIRSWLHKRKYRKKTTVS

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=271, Percent_Identity=29.8892988929889, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 45828; Mature: 45697

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLCGDCRIVLQSINDSSIDLVYMDPPFFTQKTHALVNRDGNASYSFEDTWESIETYLA
CEEEECCHHHHEECCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
FMREVLIQCKRVLKDTGSIFVHCDRSASHHLRVLLDQIFGPEHFQSEIIWTYRRWSNSKK
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHEEEEEHHCCCCC
GLLPAHQTIYFYSKTENFKFNVLFTDYSPTTNIDQILQARARNEKGKSVYRRDQHGNIVA
CCCCCCEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCEEE
GKEKKGVPLSDVWYIPFLNPKAKERVGYPTQKPVLLLERIIKIATDSGDSVLDPFCGSGT
CCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCH
TLVAAKLLGRTYIGIDISREAVSLSEERLRQPIKSESQLLVVGEEGFRMKSNYERNLLKA
HHHHHHHHCCEEEEEEECHHHHHHHHHHHHCCCCCCCCEEEECCCCCEECCCHHHHHHHH
LDAVPVERNSGIDGFLFIDEQPIPVRIQKDDEDIATARQKLKAASRAKQCSSMILIRTKT
HHCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
ENNLCLFETEDDDVLVIDAYDLIIRSWLHKRKYRKKTTVS
CCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVLCGDCRIVLQSINDSSIDLVYMDPPFFTQKTHALVNRDGNASYSFEDTWESIETYLA
EEEECCHHHHEECCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
FMREVLIQCKRVLKDTGSIFVHCDRSASHHLRVLLDQIFGPEHFQSEIIWTYRRWSNSKK
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHEEEEEHHCCCCC
GLLPAHQTIYFYSKTENFKFNVLFTDYSPTTNIDQILQARARNEKGKSVYRRDQHGNIVA
CCCCCCEEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCEEE
GKEKKGVPLSDVWYIPFLNPKAKERVGYPTQKPVLLLERIIKIATDSGDSVLDPFCGSGT
CCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCH
TLVAAKLLGRTYIGIDISREAVSLSEERLRQPIKSESQLLVVGEEGFRMKSNYERNLLKA
HHHHHHHHCCEEEEEEECHHHHHHHHHHHHCCCCCCCCEEEECCCCCEECCCHHHHHHHH
LDAVPVERNSGIDGFLFIDEQPIPVRIQKDDEDIATARQKLKAASRAKQCSSMILIRTKT
HHCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
ENNLCLFETEDDDVLVIDAYDLIIRSWLHKRKYRKKTTVS
CCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]