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The map label for this gene is pbpF [H]

Identifier: 222151654

GI number: 222151654

Start: 1523915

End: 1526263

Strand: Direct

Name: pbpF [H]

Synonym: MCCL_1407

Alternate gene names: 222151654

Gene position: 1523915-1526263 (Clockwise)

Preceding gene: 222151650

Following gene: 222151657

Centisome position: 72.49

GC content: 38.19

Gene sequence:

>2349_bases
ATGGCAAACAAAACTTCATACAAAGATAAATTTAAGCGTCAGCTTATAGGCTATAAGGAAGGATTTAAAAACATAAAATT
TACCCCCTACGTTATCTTTAACGGACTTTATGAAACTGTTTCAAAAACACTGCTCTTTCTAATCACAGCAGGCATTTTAT
TCGCTACGCTCATGTTTGGGATCGGCAGTGGCTATTTCCTGGCACTGGTGAAAGATGAGCCGGTAAAGTCTAACAGTGAA
CTGAAGAAATCACTTTATGAGATGACACAAAGCACAACTGTATATTTCGGTTCGGGAGAAACACTGGGTAATTTAAACGC
TGATACACAGCGACAAGTTGTTAAGCTTAAGGATATGAGTCCTCATGTGAAAGATGCATTGATCGCTACTGAAGATGAAA
GTTTCTATCGCCATAGAGGTATTGTACCGAAAGCTTTTATACGAGCAACTGCTCAAGAATTTCTTAATACTGGCGTGACG
AGTGGAGGCAGTACTTTAACACAGCAGCTTATCAAGAATCAGCTGTTAACAAATGAAACTTCCTTCCAAAGAAAAGCAAA
AGAAATGATGCTTTCGTTCAAAGTAGAAAAAGCACTGACGAAAGATGAAATTATCGAAGCTTATTTAAATGTCGTCAGTT
TTGGTCGAAACACGAATGGTCAGAACATTGCTGGAATCGAAGCGGCTGCTCAAGGGATTTTCGGAGTTAAAGCAAATGAG
CTTAACATCGCTCAAGCTGCATATATCGCAGGTCTTCCACAAAACCCTTATACATATACACCATTTTTGCAGGACGGCTC
AGTCAAGTCCAAAGATGATCTTGCATATGGACTGACAAGACAGCAATATGTTCTGTCACGTATGTATCAGGAAAATAAGA
TCAGTAAAGCAGAGTTTGATAAAGCAAAATCATTTAACATCGTTAAGTCATTTACGAAAGATGTAGAGACGCCAAACGAA
AAGTATCCTTTCCTGACCCAAGAAGTAGAAGATGAAGCTGTAGATATTCTGAAATATCACTTTGCTTCAAAAGATAATGT
TTCAAAGACAGAACTTGATGAAACACCCGTTCTTCAAGAAAAGTATACAAATATCGCAGAACGTGCTGTACGTAACGATG
GGTATATCGTCGAAACTACAATTAACAAGCCAATTTTCGATGCCATGAATGCAGTTAAAGATAATCCGAACTATTATTCC
TACGACCGACAAGTAGAGGTTGGCGGAAAGACAAAGTCATTGAGCCAAGAAGTCGGTGTCCTTTTAAAAGAAAATAAAAC
GGGTAAAATTATCGCGTTTGTTGGTGGTCGAGATCACGATAAGTCACAAAACAACCATGCAATGAAAACGAAGCGTTCTC
CAGGATCTACAATCAAACCATTATTAACCTATGCTCCTGCAATGGAGTATGGGATTACGGCGCCAGAAACGATGCTGCTC
GATAAAAAGTTTAATTACAATGGTTATGAACCGGAAAACTATGCACGTATGGAATATGGAAATGTGACAACCCGTTATGC
GTTAGAGAACTCTTTAAACTTAAGTACTTTAAGACTATATTCTAGTATTCAGGATAAGAAGCCTTGGGAATTATTAAACA
AGATGGACTTTAACATTCCAGAATCTGAACAGGAAAACTTATCATTAGCACTCGGTGCGACAGATATCACGCTTAAAAAT
GATGTCGATGGTTTCTCAACTTTAGCCAATGGAGGAAACTATCAAGAAAGCTATATGATCGAACGTATTAAAGCTAAAGA
TGGCAAAGTCATCTATCAGCATGAAGCAAAACCAGAGCGAATTTATAGTGCATCTACCGCTTATATGACCACTGATATGT
TACGTGGTGTTCTCGATACTGGTAGTGCTTATTCATTGAAAGGTACCTTCCTATATAATCAGGACTGGGCCGGTAAAACA
GGTACGACTCAAGATGCAAAAGACAGTCTGTTTGTTGCTTATAACCCTAAAGTAACAATGGGTATCTGGATGGGCTATGA
TGTTCCAACTACATTTGATGAAGAAAATCACTACCAGCTCAAGTTATGGCGTGACATCGTCAACCAGATTTCATCAATCG
ATCAATCCCAGATGGGTGTCGGTGAACGATTTAACCAGCCAAAGAGCGTAACAAAACAAAACATCTGTCAATTTACGAAT
AATACATCCGGCTGTGTGTCTGGTGAATCTACAAAAGAAAGTCTGGTATCTGACAAAACTGATTTAACCCAGAAAACACT
AGATACTCCTGACGTATTGAATAGACTTGGTATTAAACTAGACCCTGCAACAAAGAGTAAGATTTTTACAAAATCGAAAC
AAAATACTCAAAAGAAAAAAAGAAACTAA

Upstream 100 bases:

>100_bases
TAGTTTCAAATCAATTATGATACTAGTGTATAAAATTGGATGAGTGAAGTCAAGAACCTGAAATTATGATATACTAAAAA
AATATTCAGGAGGAAAAAAT

Downstream 100 bases:

>100_bases
AAAGAACCGGTCAGTATAGAAACGCATTGCTTTCTATACTGATCGGTTCTTCTTAATAATTATAACTGCTTGATGCAATA
TTCTATTAGAATAAACCTAC

Product: hypothetical protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 782; Mature: 781

Protein sequence:

>782_residues
MANKTSYKDKFKRQLIGYKEGFKNIKFTPYVIFNGLYETVSKTLLFLITAGILFATLMFGIGSGYFLALVKDEPVKSNSE
LKKSLYEMTQSTTVYFGSGETLGNLNADTQRQVVKLKDMSPHVKDALIATEDESFYRHRGIVPKAFIRATAQEFLNTGVT
SGGSTLTQQLIKNQLLTNETSFQRKAKEMMLSFKVEKALTKDEIIEAYLNVVSFGRNTNGQNIAGIEAAAQGIFGVKANE
LNIAQAAYIAGLPQNPYTYTPFLQDGSVKSKDDLAYGLTRQQYVLSRMYQENKISKAEFDKAKSFNIVKSFTKDVETPNE
KYPFLTQEVEDEAVDILKYHFASKDNVSKTELDETPVLQEKYTNIAERAVRNDGYIVETTINKPIFDAMNAVKDNPNYYS
YDRQVEVGGKTKSLSQEVGVLLKENKTGKIIAFVGGRDHDKSQNNHAMKTKRSPGSTIKPLLTYAPAMEYGITAPETMLL
DKKFNYNGYEPENYARMEYGNVTTRYALENSLNLSTLRLYSSIQDKKPWELLNKMDFNIPESEQENLSLALGATDITLKN
DVDGFSTLANGGNYQESYMIERIKAKDGKVIYQHEAKPERIYSASTAYMTTDMLRGVLDTGSAYSLKGTFLYNQDWAGKT
GTTQDAKDSLFVAYNPKVTMGIWMGYDVPTTFDEENHYQLKLWRDIVNQISSIDQSQMGVGERFNQPKSVTKQNICQFTN
NTSGCVSGESTKESLVSDKTDLTQKTLDTPDVLNRLGIKLDPATKSKIFTKSKQNTQKKKRN

Sequences:

>Translated_782_residues
MANKTSYKDKFKRQLIGYKEGFKNIKFTPYVIFNGLYETVSKTLLFLITAGILFATLMFGIGSGYFLALVKDEPVKSNSE
LKKSLYEMTQSTTVYFGSGETLGNLNADTQRQVVKLKDMSPHVKDALIATEDESFYRHRGIVPKAFIRATAQEFLNTGVT
SGGSTLTQQLIKNQLLTNETSFQRKAKEMMLSFKVEKALTKDEIIEAYLNVVSFGRNTNGQNIAGIEAAAQGIFGVKANE
LNIAQAAYIAGLPQNPYTYTPFLQDGSVKSKDDLAYGLTRQQYVLSRMYQENKISKAEFDKAKSFNIVKSFTKDVETPNE
KYPFLTQEVEDEAVDILKYHFASKDNVSKTELDETPVLQEKYTNIAERAVRNDGYIVETTINKPIFDAMNAVKDNPNYYS
YDRQVEVGGKTKSLSQEVGVLLKENKTGKIIAFVGGRDHDKSQNNHAMKTKRSPGSTIKPLLTYAPAMEYGITAPETMLL
DKKFNYNGYEPENYARMEYGNVTTRYALENSLNLSTLRLYSSIQDKKPWELLNKMDFNIPESEQENLSLALGATDITLKN
DVDGFSTLANGGNYQESYMIERIKAKDGKVIYQHEAKPERIYSASTAYMTTDMLRGVLDTGSAYSLKGTFLYNQDWAGKT
GTTQDAKDSLFVAYNPKVTMGIWMGYDVPTTFDEENHYQLKLWRDIVNQISSIDQSQMGVGERFNQPKSVTKQNICQFTN
NTSGCVSGESTKESLVSDKTDLTQKTLDTPDVLNRLGIKLDPATKSKIFTKSKQNTQKKKRN
>Mature_781_residues
ANKTSYKDKFKRQLIGYKEGFKNIKFTPYVIFNGLYETVSKTLLFLITAGILFATLMFGIGSGYFLALVKDEPVKSNSEL
KKSLYEMTQSTTVYFGSGETLGNLNADTQRQVVKLKDMSPHVKDALIATEDESFYRHRGIVPKAFIRATAQEFLNTGVTS
GGSTLTQQLIKNQLLTNETSFQRKAKEMMLSFKVEKALTKDEIIEAYLNVVSFGRNTNGQNIAGIEAAAQGIFGVKANEL
NIAQAAYIAGLPQNPYTYTPFLQDGSVKSKDDLAYGLTRQQYVLSRMYQENKISKAEFDKAKSFNIVKSFTKDVETPNEK
YPFLTQEVEDEAVDILKYHFASKDNVSKTELDETPVLQEKYTNIAERAVRNDGYIVETTINKPIFDAMNAVKDNPNYYSY
DRQVEVGGKTKSLSQEVGVLLKENKTGKIIAFVGGRDHDKSQNNHAMKTKRSPGSTIKPLLTYAPAMEYGITAPETMLLD
KKFNYNGYEPENYARMEYGNVTTRYALENSLNLSTLRLYSSIQDKKPWELLNKMDFNIPESEQENLSLALGATDITLKND
VDGFSTLANGGNYQESYMIERIKAKDGKVIYQHEAKPERIYSASTAYMTTDMLRGVLDTGSAYSLKGTFLYNQDWAGKTG
TTQDAKDSLFVAYNPKVTMGIWMGYDVPTTFDEENHYQLKLWRDIVNQISSIDQSQMGVGERFNQPKSVTKQNICQFTNN
TSGCVSGESTKESLVSDKTDLTQKTLDTPDVLNRLGIKLDPATKSKIFTKSKQNTQKKKRN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=351, Percent_Identity=31.0541310541311, Blast_Score=139, Evalue=9e-34,
Organism=Escherichia coli, GI1786343, Length=268, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789601, Length=188, Percent_Identity=31.9148936170213, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788867, Length=179, Percent_Identity=33.5195530726257, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 87969; Mature: 87838

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKTSYKDKFKRQLIGYKEGFKNIKFTPYVIFNGLYETVSKTLLFLITAGILFATLMFG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGSGYFLALVKDEPVKSNSELKKSLYEMTQSTTVYFGSGETLGNLNADTQRQVVKLKDMS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHEECCCC
PHVKDALIATEDESFYRHRGIVPKAFIRATAQEFLNTGVTSGGSTLTQQLIKNQLLTNET
CCHHHHEEECCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCH
SFQRKAKEMMLSFKVEKALTKDEIIEAYLNVVSFGRNTNGQNIAGIEAAAQGIFGVKANE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHCCCCCCCCCC
LNIAQAAYIAGLPQNPYTYTPFLQDGSVKSKDDLAYGLTRQQYVLSRMYQENKISKAEFD
CCHHHHHHHHCCCCCCCEECCEECCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHH
KAKSFNIVKSFTKDVETPNEKYPFLTQEVEDEAVDILKYHFASKDNVSKTELDETPVLQE
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHH
KYTNIAERAVRNDGYIVETTINKPIFDAMNAVKDNPNYYSYDRQVEVGGKTKSLSQEVGV
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEECCCCEEEECCCCHHHHHHHCE
LLKENKTGKIIAFVGGRDHDKSQNNHAMKTKRSPGSTIKPLLTYAPAMEYGITAPETMLL
EEECCCCCEEEEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHCCHHHHCCCCCCCEEE
DKKFNYNGYEPENYARMEYGNVTTRYALENSLNLSTLRLYSSIQDKKPWELLNKMDFNIP
ECCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCC
ESEQENLSLALGATDITLKNDVDGFSTLANGGNYQESYMIERIKAKDGKVIYQHEAKPER
CCCCCCEEEEECCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHH
IYSASTAYMTTDMLRGVLDTGSAYSLKGTFLYNQDWAGKTGTTQDAKDSLFVAYNPKVTM
HHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEE
GIWMGYDVPTTFDEENHYQLKLWRDIVNQISSIDQSQMGVGERFNQPKSVTKQNICQFTN
EEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCHHHCCCHHHCCCCHHHHHHHHHHHCC
NTSGCVSGESTKESLVSDKTDLTQKTLDTPDVLNRLGIKLDPATKSKIFTKSKQNTQKKK
CCCCCCCCCHHHHHHHHCHHHHHHHHCCCHHHHHHCCCEECCCCCHHHHHCCHHHHHHHC
RN
CC
>Mature Secondary Structure 
ANKTSYKDKFKRQLIGYKEGFKNIKFTPYVIFNGLYETVSKTLLFLITAGILFATLMFG
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGSGYFLALVKDEPVKSNSELKKSLYEMTQSTTVYFGSGETLGNLNADTQRQVVKLKDMS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHEECCCC
PHVKDALIATEDESFYRHRGIVPKAFIRATAQEFLNTGVTSGGSTLTQQLIKNQLLTNET
CCHHHHEEECCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCH
SFQRKAKEMMLSFKVEKALTKDEIIEAYLNVVSFGRNTNGQNIAGIEAAAQGIFGVKANE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHCCCCCCCCCC
LNIAQAAYIAGLPQNPYTYTPFLQDGSVKSKDDLAYGLTRQQYVLSRMYQENKISKAEFD
CCHHHHHHHHCCCCCCCEECCEECCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHH
KAKSFNIVKSFTKDVETPNEKYPFLTQEVEDEAVDILKYHFASKDNVSKTELDETPVLQE
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHH
KYTNIAERAVRNDGYIVETTINKPIFDAMNAVKDNPNYYSYDRQVEVGGKTKSLSQEVGV
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCEECCCCEEEECCCCHHHHHHHCE
LLKENKTGKIIAFVGGRDHDKSQNNHAMKTKRSPGSTIKPLLTYAPAMEYGITAPETMLL
EEECCCCCEEEEEECCCCCCCCCCCCCEECCCCCCCCHHHHHHHCCHHHHCCCCCCCEEE
DKKFNYNGYEPENYARMEYGNVTTRYALENSLNLSTLRLYSSIQDKKPWELLNKMDFNIP
ECCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCC
ESEQENLSLALGATDITLKNDVDGFSTLANGGNYQESYMIERIKAKDGKVIYQHEAKPER
CCCCCCEEEEECCEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHH
IYSASTAYMTTDMLRGVLDTGSAYSLKGTFLYNQDWAGKTGTTQDAKDSLFVAYNPKVTM
HHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEE
GIWMGYDVPTTFDEENHYQLKLWRDIVNQISSIDQSQMGVGERFNQPKSVTKQNICQFTN
EEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCHHHCCCHHHCCCCHHHHHHHHHHHCC
NTSGCVSGESTKESLVSDKTDLTQKTLDTPDVLNRLGIKLDPATKSKIFTKSKQNTQKKK
CCCCCCCCCHHHHHHHHCHHHHHHHHCCCHHHHHHCCCEECCCCCHHHHHCCHHHHHHHC
RN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]