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The map label for this gene is ispG

Identifier: 222151449

GI number: 222151449

Start: 1306096

End: 1307211

Strand: Direct

Name: ispG

Synonym: MCCL_1202

Alternate gene names: 222151449

Gene position: 1306096-1307211 (Clockwise)

Preceding gene: 222151448

Following gene: 222151450

Centisome position: 62.13

GC content: 38.98

Gene sequence:

>1116_bases
ATGCCTGAAATTACACATAGAAAAAATACACGACCGGTTAAAGTCGGCGATCTTACTATTGGGGGTTCAAACGAACTTGT
AATCCAAAGTATGACAACAACGAAGACACATGATGTTGAAGCTACAGTTGCAGAGATCAAACGTCTTGAAGAAGCGGGAT
GCCAGATTGTCCGTGTAGCCTGTCCTAAAGAAGAAGATGCACTTGCAATTGCAGAGATTAAAAAACAAATTAATATTCCA
TTAGTCGTCGATATTCATTTCGACTATAAACTTGCATTACTCGCAATTGAAGGTGGTGCGGATAAAATCCGTATTAATCC
GGGAAATATCGGTCGTCGTGAAAAAGTTGAAGAAGTTGTAAAAGCTTGTAAAGCGAAGGGCATTCCGATTCGTATCGGAG
TAAACGCAGGTTCACTTGAGAAACATATATTAAAAAAATATGGTTATCCTACAGCAGATGGTATGGTGGAAAGTGCATTG
CATCATATTAAAATATTAGAAGATCTGGATTTCCATGATATTATCGTTTCGATGAAAGCAAGTGATGTTAACTTAGCGAT
TGAGGCCTATACAAAAGCTGCGCAAGCATTTGATTATCCACTACATTTAGGTATCACAGAGAGCGGGACATTATTTGCCG
GGACAGTAAAATCAGCCGCAGGTCTCGGTGCAATTATGAGTTTAGGTATTGGTAACACGTTGCGTATTTCATTATCAGCA
GATCCCGTAGAAGAAGTTAAAGTAGCGAGAGAACTATTGAAATCTTTCGGTCTGGCAAGTAACGCAGCAACGCTTATTTC
ATGTCCGACTTGTGGTCGAATTGAAATCGACTTAATTTCAATTGCCAACGAAGTTGAAGAATATATTTCAACAATCAAAG
CACCACTCAAAGTCGCAGTTTTAGGATGTGCTGTAAATGGTCCTGGTGAAGCACGTGAAGCAGATATCGGTATTGCTGGT
GCGCGTGGTGAAGGTCTGTTATTTATGAAAGGTAAAACGGTAAGAAAAGTGCCCGAAGAGACAATGGTTGAAGAATTGAA
GATGGAAATCGATAAACTTGCTGAAGAATACTTTAAAAAACAGGAAGCAGAAAAATTAGCAAATGCAGAAAAATAA

Upstream 100 bases:

>100_bases
AAGCATAAAGCTAAACAAATTTGATTTCCAACTATATAATAGAAGTGTTATTCTAGTAAGGATAGTAATTAATAAGTTTA
AATTATGAAGGAGAATTATA

Downstream 100 bases:

>100_bases
AATAAGATTCGGTATTGATATCGATGGTACTGTTACTTGTCCGACAGCACTTGTGCCATACCTTCAGAAATCCTTTAATC
CAGACTTTAAGTACGAAGAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MPEITHRKNTRPVKVGDLTIGGSNELVIQSMTTTKTHDVEATVAEIKRLEEAGCQIVRVACPKEEDALAIAEIKKQINIP
LVVDIHFDYKLALLAIEGGADKIRINPGNIGRREKVEEVVKACKAKGIPIRIGVNAGSLEKHILKKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYTKAAQAFDYPLHLGITESGTLFAGTVKSAAGLGAIMSLGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTIKAPLKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFMKGKTVRKVPEETMVEELKMEIDKLAEEYFKKQEAEKLANAEK

Sequences:

>Translated_371_residues
MPEITHRKNTRPVKVGDLTIGGSNELVIQSMTTTKTHDVEATVAEIKRLEEAGCQIVRVACPKEEDALAIAEIKKQINIP
LVVDIHFDYKLALLAIEGGADKIRINPGNIGRREKVEEVVKACKAKGIPIRIGVNAGSLEKHILKKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYTKAAQAFDYPLHLGITESGTLFAGTVKSAAGLGAIMSLGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTIKAPLKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFMKGKTVRKVPEETMVEELKMEIDKLAEEYFKKQEAEKLANAEK
>Mature_370_residues
PEITHRKNTRPVKVGDLTIGGSNELVIQSMTTTKTHDVEATVAEIKRLEEAGCQIVRVACPKEEDALAIAEIKKQINIPL
VVDIHFDYKLALLAIEGGADKIRINPGNIGRREKVEEVVKACKAKGIPIRIGVNAGSLEKHILKKYGYPTADGMVESALH
HIKILEDLDFHDIIVSMKASDVNLAIEAYTKAAQAFDYPLHLGITESGTLFAGTVKSAAGLGAIMSLGIGNTLRISLSAD
PVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTIKAPLKVAVLGCAVNGPGEAREADIGIAGA
RGEGLLFMKGKTVRKVPEETMVEELKMEIDKLAEEYFKKQEAEKLANAEK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=347, Percent_Identity=48.9913544668588, Blast_Score=337, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_MACCJ (B9E6U1)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560605.1
- GeneID:   7389158
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_1202
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40002; Mature: 39871

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEITHRKNTRPVKVGDLTIGGSNELVIQSMTTTKTHDVEATVAEIKRLEEAGCQIVRVA
CCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPKEEDALAIAEIKKQINIPLVVDIHFDYKLALLAIEGGADKIRINPGNIGRREKVEEVV
CCCCCCCHHHHHHHHHCCCCEEEEEECCEEEEEEEEECCCCEEEECCCCCCHHHHHHHHH
KACKAKGIPIRIGVNAGSLEKHILKKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYTKAAQAFDYPLHLGITESGTLFAGTVKSAAGLGAIMSLGIGNTLRISLSA
CCCEEEHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTIKAPLKVAV
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFMKGKTVRKVPEETMVEELKMEIDKLAEEYFKK
EEEEECCCCCCCCCCCEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QEAEKLANAEK
HHHHHHHCCCC
>Mature Secondary Structure 
PEITHRKNTRPVKVGDLTIGGSNELVIQSMTTTKTHDVEATVAEIKRLEEAGCQIVRVA
CCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPKEEDALAIAEIKKQINIPLVVDIHFDYKLALLAIEGGADKIRINPGNIGRREKVEEVV
CCCCCCCHHHHHHHHHCCCCEEEEEECCEEEEEEEEECCCCEEEECCCCCCHHHHHHHHH
KACKAKGIPIRIGVNAGSLEKHILKKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYTKAAQAFDYPLHLGITESGTLFAGTVKSAAGLGAIMSLGIGNTLRISLSA
CCCEEEHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTIKAPLKVAV
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFMKGKTVRKVPEETMVEELKMEIDKLAEEYFKK
EEEEECCCCCCCCCCCEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QEAEKLANAEK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA