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The map label for this gene is grlA [H]

Identifier: 222151259

GI number: 222151259

Start: 1116213

End: 1118624

Strand: Direct

Name: grlA [H]

Synonym: MCCL_1012

Alternate gene names: 222151259

Gene position: 1116213-1118624 (Clockwise)

Preceding gene: 222151258

Following gene: 222151260

Centisome position: 53.09

GC content: 36.94

Gene sequence:

>2412_bases
ATGAATGAGAATATACAAAGTCTTCCACTTGAAGATGTAATCGGTGATCGTTTTGGTCGCTATAGTAAATATATTATACA
GGATCGAGCGATTCCGGATGTACGTGATGGGCTTAAGCCAGTTCAACGTCGTATTTTATTTGCGATGTATGCAGAAGGCA
ATACATATGATAAGAACTACAGAAAAAGTGCGAAAACGGTTGGTAATGTTATCGGAAACTATCATCCTCACGGAGATACA
TCTGTTTATGATGCAATGGTCCGTATGAGTCAGGACTGGAAACTGAGACATGTCCTTGTTGAGATGCATGGTAATAACGG
AAGTATCGATAATGATCCACCTGCAGCGATGCGTTATACTGAAGCTAAGTTATCTAAGCTTGCTAACATCTTGCTTGAAG
ATATCAATAAAGAGACGGTTGATTTTGTACAGAACTTTGATGATACTGCGATGGAGCCGATGGTATTACCGGCAAACTAC
CCGAATCTACTCGTTAATGGATCAACTGGTATTTCAGCTGGATATGCTACGGATATACCTCCACACAATCTAGATGAAGT
GATTAATGCGACATTAAAGGTCATAGATAAACCGAATATATCTACTGATGAAATAATGAAGGTGCTGCCAGGACCGGATT
TCCCGACGGGCGGCATAATAATGGGGAAATCAGGTATCAAACAGGCATACGAGACAGGAAGAGGTAAGATACAGGTTCGT
GGGAAAGTAGATATTGAAAAATTACGTGGTGGGAGAGAAGAGATTATTATCTCAGAAATTCCATTTGAAGTGAATAAGAG
TAACCTTGTTAAGCGCATTGATGAACTGCGTGCAGATAAGAAGGTCGACGGTATTATCGAGGTACGTGATGAAACGGATC
GAAATGGTTTGCGGATTGCTATCGAGTTGAAGAAGGATGCAAATAGTGAAGGCATTCTTAATTTCCTTTATAAAAATACA
GACCTACAGATTGCATATAACTTCAATATGGTAGCAATCAGTGATCGCCGACCGAAACTGCTAGGTGTCCGTGATATGCT
TAACAGTTTTATCAGTCATCAAAAAGAAGTTGTTACAAGACGCAGTCAATATGACTATGATCACGCTGAGAAGCGTATGC
ATATTGTTGAGGGATTGATGAAAGCACTTTCGATATTAGATCAGGTAATTAAAGTCATCCGTAATTCGTCAAATAAAGCT
GATGCAAAACACAACTTGATACATAAGTTTAATTTTACTGAAGCACAGGCTGAAGCAATCGTTATGCTGCAACTTTATCG
ATTAACCAATACAGATATTGTGGCATTAGAAGCAGAACATGAAGCATTAAAAGATAGACTCAAGCAGTTATCACTTATAT
TGAATGATGAGACGCATCTGAAAAAAGTGATCAAAAAAGAATTAAAAGCAATCCAAAAAGAATTTAAAGAAGAGCGTCTG
AGTCAGATTGAAGAAAAAATTGCAGAAATTAAGATATCTAAGGAAATCCTTGTTCCTAATGAAGAAACAGTGATCAGTAT
TACAAGGGAAGGTTACATAAAACGCACATCTCCACGTAGTTTTAATGCTAGTGGTGTAACCGATATTGGTATGAAAGAAG
ACGATCAGTTACTGGCTTATCATGAAAGCAATCTGCAGCATATAACGTTAGTATTTACAGATAAAGGACGATATATGTAT
ATTCCAAATCATCAGTTACCGGAAATCAGATGGAAAGATCTTGGAACGCATATTTCTCAGATTGTACCAATAGATAGCGA
TGAGCGTGTGATTCATATGATTACCGTCACTGACTTCAATTCAGAGGTGAAAATTTTAGCTGCTACAAGAAATGGTATGA
TAAAGTTATCTTCGTTGAAGGACTTTGAAGTTACAAGATATTCTAGACCACTTATCAATATGAAGCTAAAAGGTAATGAT
GTTGTGAATACTATCCATGTAGTAACAGGCAGTGAACATATTCTTGTCGTTACGAAGCAAGGCATGAGTCTGCATTATTC
ATTAACTGAACTTAATGATACAGGACTTAAAGCTGCAGGTGTTAAATCGATCAATCTTAAAGATGATGATGTTGTCATAA
CAACGGAAATCATCTATCCAGAAAGTTCATTGCTGATTGCGACACAACGAGGTGCAATGAAAAAAATGGATATTCAATTA
TTTGAAGAAGCTAAGCGTGCCCAACGTGGCTTAATGATACTGAAAGAATTAAAAAGTAATCCTCACCATATTATCGGTGC
ATACATCATCACTGAAGATATGGCATATACACTCGTATCGGAACACCATGAAAAACATGGAAAAACAAAAGCAATCAGAA
AAACAGATCGTTACACTAATGGCAGCTTTATCATCGATGAGAAAGATTTTGGTAAAGTCGTTAAACTTTATATAAATTCT
GTAGATTTATAA

Upstream 100 bases:

>100_bases
ATGCAGGAAGATGTAAGCATTCTGGAAAACGATGAAGTCGAACGTTTAACAGAAATAGAGGATATCGAAACAATTCAGGA
TGATGAAAAGGGTGAGGATA

Downstream 100 bases:

>100_bases
TGTTTGTGTAGACCCTTTCATTATCTATGGTAAAATAGTTTTAGTTTTATTTTACTTTGAATAGTGGAGGGTTTTTATTT
TGAATATATCAAAAGTAAAT

Product: DNA topoisomerase IV subunit A

Products: NA

Alternate protein names: Topoisomerase IV subunit A [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MNENIQSLPLEDVIGDRFGRYSKYIIQDRAIPDVRDGLKPVQRRILFAMYAEGNTYDKNYRKSAKTVGNVIGNYHPHGDT
SVYDAMVRMSQDWKLRHVLVEMHGNNGSIDNDPPAAMRYTEAKLSKLANILLEDINKETVDFVQNFDDTAMEPMVLPANY
PNLLVNGSTGISAGYATDIPPHNLDEVINATLKVIDKPNISTDEIMKVLPGPDFPTGGIIMGKSGIKQAYETGRGKIQVR
GKVDIEKLRGGREEIIISEIPFEVNKSNLVKRIDELRADKKVDGIIEVRDETDRNGLRIAIELKKDANSEGILNFLYKNT
DLQIAYNFNMVAISDRRPKLLGVRDMLNSFISHQKEVVTRRSQYDYDHAEKRMHIVEGLMKALSILDQVIKVIRNSSNKA
DAKHNLIHKFNFTEAQAEAIVMLQLYRLTNTDIVALEAEHEALKDRLKQLSLILNDETHLKKVIKKELKAIQKEFKEERL
SQIEEKIAEIKISKEILVPNEETVISITREGYIKRTSPRSFNASGVTDIGMKEDDQLLAYHESNLQHITLVFTDKGRYMY
IPNHQLPEIRWKDLGTHISQIVPIDSDERVIHMITVTDFNSEVKILAATRNGMIKLSSLKDFEVTRYSRPLINMKLKGND
VVNTIHVVTGSEHILVVTKQGMSLHYSLTELNDTGLKAAGVKSINLKDDDVVITTEIIYPESSLLIATQRGAMKKMDIQL
FEEAKRAQRGLMILKELKSNPHHIIGAYIITEDMAYTLVSEHHEKHGKTKAIRKTDRYTNGSFIIDEKDFGKVVKLYINS
VDL

Sequences:

>Translated_803_residues
MNENIQSLPLEDVIGDRFGRYSKYIIQDRAIPDVRDGLKPVQRRILFAMYAEGNTYDKNYRKSAKTVGNVIGNYHPHGDT
SVYDAMVRMSQDWKLRHVLVEMHGNNGSIDNDPPAAMRYTEAKLSKLANILLEDINKETVDFVQNFDDTAMEPMVLPANY
PNLLVNGSTGISAGYATDIPPHNLDEVINATLKVIDKPNISTDEIMKVLPGPDFPTGGIIMGKSGIKQAYETGRGKIQVR
GKVDIEKLRGGREEIIISEIPFEVNKSNLVKRIDELRADKKVDGIIEVRDETDRNGLRIAIELKKDANSEGILNFLYKNT
DLQIAYNFNMVAISDRRPKLLGVRDMLNSFISHQKEVVTRRSQYDYDHAEKRMHIVEGLMKALSILDQVIKVIRNSSNKA
DAKHNLIHKFNFTEAQAEAIVMLQLYRLTNTDIVALEAEHEALKDRLKQLSLILNDETHLKKVIKKELKAIQKEFKEERL
SQIEEKIAEIKISKEILVPNEETVISITREGYIKRTSPRSFNASGVTDIGMKEDDQLLAYHESNLQHITLVFTDKGRYMY
IPNHQLPEIRWKDLGTHISQIVPIDSDERVIHMITVTDFNSEVKILAATRNGMIKLSSLKDFEVTRYSRPLINMKLKGND
VVNTIHVVTGSEHILVVTKQGMSLHYSLTELNDTGLKAAGVKSINLKDDDVVITTEIIYPESSLLIATQRGAMKKMDIQL
FEEAKRAQRGLMILKELKSNPHHIIGAYIITEDMAYTLVSEHHEKHGKTKAIRKTDRYTNGSFIIDEKDFGKVVKLYINS
VDL
>Mature_803_residues
MNENIQSLPLEDVIGDRFGRYSKYIIQDRAIPDVRDGLKPVQRRILFAMYAEGNTYDKNYRKSAKTVGNVIGNYHPHGDT
SVYDAMVRMSQDWKLRHVLVEMHGNNGSIDNDPPAAMRYTEAKLSKLANILLEDINKETVDFVQNFDDTAMEPMVLPANY
PNLLVNGSTGISAGYATDIPPHNLDEVINATLKVIDKPNISTDEIMKVLPGPDFPTGGIIMGKSGIKQAYETGRGKIQVR
GKVDIEKLRGGREEIIISEIPFEVNKSNLVKRIDELRADKKVDGIIEVRDETDRNGLRIAIELKKDANSEGILNFLYKNT
DLQIAYNFNMVAISDRRPKLLGVRDMLNSFISHQKEVVTRRSQYDYDHAEKRMHIVEGLMKALSILDQVIKVIRNSSNKA
DAKHNLIHKFNFTEAQAEAIVMLQLYRLTNTDIVALEAEHEALKDRLKQLSLILNDETHLKKVIKKELKAIQKEFKEERL
SQIEEKIAEIKISKEILVPNEETVISITREGYIKRTSPRSFNASGVTDIGMKEDDQLLAYHESNLQHITLVFTDKGRYMY
IPNHQLPEIRWKDLGTHISQIVPIDSDERVIHMITVTDFNSEVKILAATRNGMIKLSSLKDFEVTRYSRPLINMKLKGND
VVNTIHVVTGSEHILVVTKQGMSLHYSLTELNDTGLKAAGVKSINLKDDDVVITTEIIYPESSLLIATQRGAMKKMDIQL
FEEAKRAQRGLMILKELKSNPHHIIGAYIITEDMAYTLVSEHHEKHGKTKAIRKTDRYTNGSFIIDEKDFGKVVKLYINS
VDL

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=195, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI19913408, Length=181, Percent_Identity=27.0718232044199, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1788562, Length=787, Percent_Identity=37.3570520965692, Blast_Score=547, Evalue=1e-156,
Organism=Escherichia coli, GI1789396, Length=748, Percent_Identity=32.3529411764706, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=180, Percent_Identity=28.8888888888889, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=194, Percent_Identity=26.2886597938144, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17136538, Length=175, Percent_Identity=29.7142857142857, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760
- InterPro:   IPR005741 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: 5.99.1.-

Molecular weight: Translated: 91192; Mature: 91192

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENIQSLPLEDVIGDRFGRYSKYIIQDRAIPDVRDGLKPVQRRILFAMYAEGNTYDKNY
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCHHH
RKSAKTVGNVIGNYHPHGDTSVYDAMVRMSQDWKLRHVLVEMHGNNGSIDNDPPAAMRYT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHH
EAKLSKLANILLEDINKETVDFVQNFDDTAMEPMVLPANYPNLLVNGSTGISAGYATDIP
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCC
PHNLDEVINATLKVIDKPNISTDEIMKVLPGPDFPTGGIIMGKSGIKQAYETGRGKIQVR
CCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCCEEEEE
GKVDIEKLRGGREEIIISEIPFEVNKSNLVKRIDELRADKKVDGIIEVRDETDRNGLRIA
CCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEE
IELKKDANSEGILNFLYKNTDLQIAYNFNMVAISDRRPKLLGVRDMLNSFISHQKEVVTR
EEEECCCCCCCCEEEEEECCCEEEEEEEEEEEECCCCCCEECHHHHHHHHHHHHHHHHHH
RSQYDYDHAEKRMHIVEGLMKALSILDQVIKVIRNSSNKADAKHNLIHKFNFTEAQAEAI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEEECCCHHHHHHE
VMLQLYRLTNTDIVALEAEHEALKDRLKQLSLILNDETHLKKVIKKELKAIQKEFKEERL
EEEHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SQIEEKIAEIKISKEILVPNEETVISITREGYIKRTSPRSFNASGVTDIGMKEDDQLLAY
HHHHHHHHHEEECCEEECCCCCEEEEEECCCCEEECCCCCCCCCCCEECCCCCCCCEEEE
HESNLQHITLVFTDKGRYMYIPNHQLPEIRWKDLGTHISQIVPIDSDERVIHMITVTDFN
ECCCCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEEEEEECCC
SEVKILAATRNGMIKLSSLKDFEVTRYSRPLINMKLKGNDVVNTIHVVTGSEHILVVTKQ
CCEEEEEECCCCEEEECCCCCCCHHHCCCCEEEEEECCCCEEEEEEEEECCCEEEEEEEC
GMSLHYSLTELNDTGLKAAGVKSINLKDDDVVITTEIIYPESSLLIATQRGAMKKMDIQL
CCEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHH
FEEAKRAQRGLMILKELKSNPHHIIGAYIITEDMAYTLVSEHHEKHGKTKAIRKTDRYTN
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCC
GSFIIDEKDFGKVVKLYINSVDL
CCEEEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNENIQSLPLEDVIGDRFGRYSKYIIQDRAIPDVRDGLKPVQRRILFAMYAEGNTYDKNY
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCHHH
RKSAKTVGNVIGNYHPHGDTSVYDAMVRMSQDWKLRHVLVEMHGNNGSIDNDPPAAMRYT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHH
EAKLSKLANILLEDINKETVDFVQNFDDTAMEPMVLPANYPNLLVNGSTGISAGYATDIP
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCC
PHNLDEVINATLKVIDKPNISTDEIMKVLPGPDFPTGGIIMGKSGIKQAYETGRGKIQVR
CCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCCEEEEE
GKVDIEKLRGGREEIIISEIPFEVNKSNLVKRIDELRADKKVDGIIEVRDETDRNGLRIA
CCCCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEE
IELKKDANSEGILNFLYKNTDLQIAYNFNMVAISDRRPKLLGVRDMLNSFISHQKEVVTR
EEEECCCCCCCCEEEEEECCCEEEEEEEEEEEECCCCCCEECHHHHHHHHHHHHHHHHHH
RSQYDYDHAEKRMHIVEGLMKALSILDQVIKVIRNSSNKADAKHNLIHKFNFTEAQAEAI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEEECCCHHHHHHE
VMLQLYRLTNTDIVALEAEHEALKDRLKQLSLILNDETHLKKVIKKELKAIQKEFKEERL
EEEHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SQIEEKIAEIKISKEILVPNEETVISITREGYIKRTSPRSFNASGVTDIGMKEDDQLLAY
HHHHHHHHHEEECCEEECCCCCEEEEEECCCCEEECCCCCCCCCCCEECCCCCCCCEEEE
HESNLQHITLVFTDKGRYMYIPNHQLPEIRWKDLGTHISQIVPIDSDERVIHMITVTDFN
ECCCCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEEEEEECCC
SEVKILAATRNGMIKLSSLKDFEVTRYSRPLINMKLKGNDVVNTIHVVTGSEHILVVTKQ
CCEEEEEECCCCEEEECCCCCCCHHHCCCCEEEEEECCCCEEEEEEEEECCCEEEEEEEC
GMSLHYSLTELNDTGLKAAGVKSINLKDDDVVITTEIIYPESSLLIATQRGAMKKMDIQL
CCEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHH
FEEAKRAQRGLMILKELKSNPHHIIGAYIITEDMAYTLVSEHHEKHGKTKAIRKTDRYTN
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCC
GSFIIDEKDFGKVVKLYINSVDL
CCEEEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA