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The map label for this gene is miaA

Identifier: 222151144

GI number: 222151144

Start: 989546

End: 990484

Strand: Direct

Name: miaA

Synonym: MCCL_0895

Alternate gene names: 222151144

Gene position: 989546-990484 (Clockwise)

Preceding gene: 222151143

Following gene: 222151146

Centisome position: 47.07

GC content: 38.23

Gene sequence:

>939_bases
ATGAATAAAATTCCGTTAATCGTTATTGTGGGACCCACAGCTGTAGGTAAAACAGCATTAAGTATTGAAATAGCTAAAGC
TGTTAATGGTGAAATTATTAGCGGGGATGCGATTCAGGTCTACCGAGGTATGGATATCGGTAGTGCAAAGATTACACAGG
AAGAGATGGAAGGCATCCCACATCATCTGATTGATATTTTAAACCCAGATGAGGCATATTCAGCAGCACAGTTTAAAGTC
CATGCTGAAAAGCTGATTGAAGACATATATAGCAGAGGAAAAACACCGATGATTGTCGGCGGAACGGGATTATATATACA
AAGCGTACTATATGAATATGAATTTGTAGAAGAGGATCAAGCGCTGAAGAAAGAACTGATGGATCACTATGAAAGTCTGG
ATAAAGAGACGCTTTATCGTCTGTTGACTGAGAAGGATACAAAGGCTGCGTCTCAAATTCATATTAATAATAGACAGCGA
GTGTTACGCGCGCTGACATATTATGAAATGCACCATAAATCTATCACTGATCAGAAGAAGAGTCATACATTAAGTTCTAA
ATACGATACATATATTATAGGGCTTAATATGCCGCGTCCAACACTATATGACAGAATTAACCGCCGTGTATTGCTTATGG
TCGAACAAGGACTTGTTCAGGAAGTGGAGACATTGATAAGTAAGGGCTATAGACAGAGCCAAAGCATGACTGCAATAGGA
TATAAAGAGATGATTCCTTATATAGATGGAGAAGTTTCGTTGAATCAGGCGATAGAAAATTTACAGCAAAACTCCAGGAA
TTTTGCAAAACGTCAATTGACATGGTTCAATAATCAGATGACGATTGAATGGTTTGATACGTCTGAATTGACGATAGAAT
CAATCGTCGAACAAATTGCTGCAAACATACCGAGGGATGATAATGATGAATTCAGCTAA

Upstream 100 bases:

>100_bases
AAACATGTCTTTATCCAGCTCTATGAAAATTCAAGACATGAGGTATTATTTGAAAAGAATCAAAAAGAAGTATTACAAAA
TATTAGAAAGTGGTTGAAGA

Downstream 100 bases:

>100_bases
CCTGCAGGACGCATTTTTAGATAATTCCAAAGAAATCAGCAAGAACTAACAGTATTTTCAACAAACGGGTTTCATATAGA
AGGTGTAGTACTCAACTATG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNKIPLIVIVGPTAVGKTALSIEIAKAVNGEIISGDAIQVYRGMDIGSAKITQEEMEGIPHHLIDILNPDEAYSAAQFKV
HAEKLIEDIYSRGKTPMIVGGTGLYIQSVLYEYEFVEEDQALKKELMDHYESLDKETLYRLLTEKDTKAASQIHINNRQR
VLRALTYYEMHHKSITDQKKSHTLSSKYDTYIIGLNMPRPTLYDRINRRVLLMVEQGLVQEVETLISKGYRQSQSMTAIG
YKEMIPYIDGEVSLNQAIENLQQNSRNFAKRQLTWFNNQMTIEWFDTSELTIESIVEQIAANIPRDDNDEFS

Sequences:

>Translated_312_residues
MNKIPLIVIVGPTAVGKTALSIEIAKAVNGEIISGDAIQVYRGMDIGSAKITQEEMEGIPHHLIDILNPDEAYSAAQFKV
HAEKLIEDIYSRGKTPMIVGGTGLYIQSVLYEYEFVEEDQALKKELMDHYESLDKETLYRLLTEKDTKAASQIHINNRQR
VLRALTYYEMHHKSITDQKKSHTLSSKYDTYIIGLNMPRPTLYDRINRRVLLMVEQGLVQEVETLISKGYRQSQSMTAIG
YKEMIPYIDGEVSLNQAIENLQQNSRNFAKRQLTWFNNQMTIEWFDTSELTIESIVEQIAANIPRDDNDEFS
>Mature_312_residues
MNKIPLIVIVGPTAVGKTALSIEIAKAVNGEIISGDAIQVYRGMDIGSAKITQEEMEGIPHHLIDILNPDEAYSAAQFKV
HAEKLIEDIYSRGKTPMIVGGTGLYIQSVLYEYEFVEEDQALKKELMDHYESLDKETLYRLLTEKDTKAASQIHINNRQR
VLRALTYYEMHHKSITDQKKSHTLSSKYDTYIIGLNMPRPTLYDRINRRVLLMVEQGLVQEVETLISKGYRQSQSMTAIG
YKEMIPYIDGEVSLNQAIENLQQNSRNFAKRQLTWFNNQMTIEWFDTSELTIESIVEQIAANIPRDDNDEFS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=28.2958199356913, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1790613, Length=285, Percent_Identity=34.7368421052632, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25144712, Length=305, Percent_Identity=30.8196721311475, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24649830, Length=340, Percent_Identity=29.1176470588235, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_MACCJ (B9EBJ1)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560298.1
- ProteinModelPortal:   B9EBJ1
- GeneID:   7390311
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0895
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35699; Mature: 35699

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIPLIVIVGPTAVGKTALSIEIAKAVNGEIISGDAIQVYRGMDIGSAKITQEEMEGIP
CCCCCEEEEECCCCCCCHHHEEEEHHHCCCCEECCCCEEEEECCCCCCHHCCHHHHHCCC
HHLIDILNPDEAYSAAQFKVHAEKLIEDIYSRGKTPMIVGGTGLYIQSVLYEYEFVEEDQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHH
ALKKELMDHYESLDKETLYRLLTEKDTKAASQIHINNRQRVLRALTYYEMHHKSITDQKK
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHH
SHTLSSKYDTYIIGLNMPRPTLYDRINRRVLLMVEQGLVQEVETLISKGYRQSQSMTAIG
HCCCCCCCCEEEEEECCCCCHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCHHCCCEEHHH
YKEMIPYIDGEVSLNQAIENLQQNSRNFAKRQLTWFNNQMTIEWFDTSELTIESIVEQIA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCHHHHHHHHHHH
ANIPRDDNDEFS
HHCCCCCCCCCC
>Mature Secondary Structure
MNKIPLIVIVGPTAVGKTALSIEIAKAVNGEIISGDAIQVYRGMDIGSAKITQEEMEGIP
CCCCCEEEEECCCCCCCHHHEEEEHHHCCCCEECCCCEEEEECCCCCCHHCCHHHHHCCC
HHLIDILNPDEAYSAAQFKVHAEKLIEDIYSRGKTPMIVGGTGLYIQSVLYEYEFVEEDQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHH
ALKKELMDHYESLDKETLYRLLTEKDTKAASQIHINNRQRVLRALTYYEMHHKSITDQKK
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHH
SHTLSSKYDTYIIGLNMPRPTLYDRINRRVLLMVEQGLVQEVETLISKGYRQSQSMTAIG
HCCCCCCCCEEEEEECCCCCHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCHHCCCEEHHH
YKEMIPYIDGEVSLNQAIENLQQNSRNFAKRQLTWFNNQMTIEWFDTSELTIESIVEQIA
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCHHHHHHHHHHH
ANIPRDDNDEFS
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA