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The map label for this gene is cvfA [H]

Identifier: 222151125

GI number: 222151125

Start: 964429

End: 965988

Strand: Direct

Name: cvfA [H]

Synonym: MCCL_0876

Alternate gene names: 222151125

Gene position: 964429-965988 (Clockwise)

Preceding gene: 222151124

Following gene: 222151126

Centisome position: 45.87

GC content: 39.87

Gene sequence:

>1560_bases
GTGAGCTTAACTATCCTCTTATTCATTTTGCTTAGTATCATAATCGGTGTTGCGATCGGTTATTTTGTTGCGAAGAACGT
ATTAACAAATCAGCAGATTCAGGCAAGAACAACTGCGGATTTTATCGTTGCTGAGGCCAACAAAGAAGCCGAAAGTCTTA
AGAAAGAGAAACTTCTTGAAGCGAAGGAAGAATACCAAAGACGTAATGACCGTCTGGAAGAAGATTATAAGGAAAGACGT
TCAGACTTAGCGAGACAAGAAGCAAGACTCCTTCAAAAAGAAGAGAACTTAGATCGTAAAAGTGACCTTTTAGATAAAAA
GGATGATCTTTTAGAACAAAAAGAATCTAAAATTGAAGAAAAGCAACAGCATGTAGATGCGTTAGAGCAAAGTGTTCAAG
GAATCATAGCACAGCATGAACAAGAATTAACACGCATCTCCGGACTGACACAAGAAGAAGCGAAGCAAGAAATTATTGAT
CAGGTTGAAGAAGAATTGAGGCATGATATTGCAGTTATGGTAAAAGAGAAGGAGCAAGAAGCAAAGCAGAATGTTGATAA
AAAAGCGAAAATGCTTCTAGCTACGACTGTACAGCGATTAGCTGCTGAACATACTTCTGAATCTACAGTGTCTGTAATTA
ACCTTCCAAATGATGAGATGAAAGGTAGAATCATTGGCCGAGAAGGTCGTAATATTCGTACGTTGGAAACGCTAACAGGT
GTTGATCTTATCATCGATGATACACCAGAAGCGGTCATCTTATCCGGATTCGATCCAATTAGAAGAGAAATTGCGAAAAC
AGCGCTTGAATCTTTAGTGTCTGATGGAAGAATTCATCCTGGCCGTATCGAAGAGATGGTTGAAAAGGCAAGAAAATCTG
TGGATGAGATTATACGAGAAGCAGGAGAAGCTGCAACGTTTGAACTAGGTATACATAATCTGCATCCTGATATCGTGAAG
ATACTTGGACGAATGAAATATCGTACGAGTTACGGACAGAATGTACTGAAACATTCAATAGAAGTTGCACACCTTTCTGG
TATGTTAGCTGCAGAACTAGGACAAGACGTTGCACTCGCTAAGCGTGCGGGACTTTTACATGATATTGGAAAAGCGATTG
ATCATGAAGTAGAGGGATCGCATGTTGAAATCGGGGTAGATCTTGCAAAGAAATATCGTGAACATCCAACAGTGATTAAC
GCCATTCATTCACATCACGGAGACGTAGAACCAGACAATATAATTTCTATTCTTGTGGCAGCAGCAGATGCGTTAAGCGC
AGCACGCCCAGGTGCCAGACGTGAAACACTTGAGAATTATGTACGTCGCTTAAACCGTCTGGAAGAAATTTCTGAGAGCT
ATGAAGGTGTAGAGAAATCATTTGCAATTCAGGCAGGTAGAGAAATCAGAATCATTGTGACACCTGAAGAAGTCGATGAT
TTAAAAGCACATCGTATCGCAAGAGATATTAAAAACCAGATTGAAGAAGAGCTTCAATATCCTGGACATATAAAAGTAAC
TGTTGTGCGAGAGATGCGTGCAGTTGAATATGCAAAATAG

Upstream 100 bases:

>100_bases
CAGCTTGACGAGTATAACTCATAAACGTACAATTAATTTATATGGATATACTTCATATAATGTTTTATTGATATATAAAA
GCAAATAAGGAGGTGTTTAT

Downstream 100 bases:

>100_bases
GTCGTGACACGAGCGGTCAGACATGAGTAGAATCAAAAAAAGATGACTAGAAATGTTATATTTCTAGTCATCTTTTTTAC
ATTGTGGACCAGTATTGCAT

Product: phosphodiesterase

Products: NA

Alternate protein names: Conserved virulence factor A [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSLTILLFILLSIIIGVAIGYFVAKNVLTNQQIQARTTADFIVAEANKEAESLKKEKLLEAKEEYQRRNDRLEEDYKERR
SDLARQEARLLQKEENLDRKSDLLDKKDDLLEQKESKIEEKQQHVDALEQSVQGIIAQHEQELTRISGLTQEEAKQEIID
QVEEELRHDIAVMVKEKEQEAKQNVDKKAKMLLATTVQRLAAEHTSESTVSVINLPNDEMKGRIIGREGRNIRTLETLTG
VDLIIDDTPEAVILSGFDPIRREIAKTALESLVSDGRIHPGRIEEMVEKARKSVDEIIREAGEAATFELGIHNLHPDIVK
ILGRMKYRTSYGQNVLKHSIEVAHLSGMLAAELGQDVALAKRAGLLHDIGKAIDHEVEGSHVEIGVDLAKKYREHPTVIN
AIHSHHGDVEPDNIISILVAAADALSAARPGARRETLENYVRRLNRLEEISESYEGVEKSFAIQAGREIRIIVTPEEVDD
LKAHRIARDIKNQIEEELQYPGHIKVTVVREMRAVEYAK

Sequences:

>Translated_519_residues
MSLTILLFILLSIIIGVAIGYFVAKNVLTNQQIQARTTADFIVAEANKEAESLKKEKLLEAKEEYQRRNDRLEEDYKERR
SDLARQEARLLQKEENLDRKSDLLDKKDDLLEQKESKIEEKQQHVDALEQSVQGIIAQHEQELTRISGLTQEEAKQEIID
QVEEELRHDIAVMVKEKEQEAKQNVDKKAKMLLATTVQRLAAEHTSESTVSVINLPNDEMKGRIIGREGRNIRTLETLTG
VDLIIDDTPEAVILSGFDPIRREIAKTALESLVSDGRIHPGRIEEMVEKARKSVDEIIREAGEAATFELGIHNLHPDIVK
ILGRMKYRTSYGQNVLKHSIEVAHLSGMLAAELGQDVALAKRAGLLHDIGKAIDHEVEGSHVEIGVDLAKKYREHPTVIN
AIHSHHGDVEPDNIISILVAAADALSAARPGARRETLENYVRRLNRLEEISESYEGVEKSFAIQAGREIRIIVTPEEVDD
LKAHRIARDIKNQIEEELQYPGHIKVTVVREMRAVEYAK
>Mature_518_residues
SLTILLFILLSIIIGVAIGYFVAKNVLTNQQIQARTTADFIVAEANKEAESLKKEKLLEAKEEYQRRNDRLEEDYKERRS
DLARQEARLLQKEENLDRKSDLLDKKDDLLEQKESKIEEKQQHVDALEQSVQGIIAQHEQELTRISGLTQEEAKQEIIDQ
VEEELRHDIAVMVKEKEQEAKQNVDKKAKMLLATTVQRLAAEHTSESTVSVINLPNDEMKGRIIGREGRNIRTLETLTGV
DLIIDDTPEAVILSGFDPIRREIAKTALESLVSDGRIHPGRIEEMVEKARKSVDEIIREAGEAATFELGIHNLHPDIVKI
LGRMKYRTSYGQNVLKHSIEVAHLSGMLAAELGQDVALAKRAGLLHDIGKAIDHEVEGSHVEIGVDLAKKYREHPTVINA
IHSHHGDVEPDNIISILVAAADALSAARPGARRETLENYVRRLNRLEEISESYEGVEKSFAIQAGREIRIIVTPEEVDDL
KAHRIARDIKNQIEEELQYPGHIKVTVVREMRAVEYAK

Specific function: Catalyzes the hydrolysis of both 2',3'-cyclic AMP and 2',3'-cyclic GMP into 3'-AMP and 3'-GMP, respectively, at the 3'- terminal of RNA. Activates sarZ and agr operon resulting in the expression of virulence genes such as hemolysin, DNase and protease [H]

COG id: COG1418

COG function: function code R; Predicted HD superfamily hydrolase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017705
- InterPro:   IPR022711
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR003607
- InterPro:   IPR006675 [H]

Pfam domain/function: PF12072 DUF3552; PF00013 KH_1 [H]

EC number: =3.1.4.16 [H]

Molecular weight: Translated: 58658; Mature: 58526

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTILLFILLSIIIGVAIGYFVAKNVLTNQQIQARTTADFIVAEANKEAESLKKEKLLE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHH
AKEEYQRRNDRLEEDYKERRSDLARQEARLLQKEENLDRKSDLLDKKDDLLEQKESKIEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHH
KQQHVDALEQSVQGIIAQHEQELTRISGLTQEEAKQEIIDQVEEELRHDIAVMVKEKEQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKQNVDKKAKMLLATTVQRLAAEHTSESTVSVINLPNDEMKGRIIGREGRNIRTLETLTG
HHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCCEECCCCCCCCHHHHHCC
VDLIIDDTPEAVILSGFDPIRREIAKTALESLVSDGRIHPGRIEEMVEKARKSVDEIIRE
CEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AGEAATFELGIHNLHPDIVKILGRMKYRTSYGQNVLKHSIEVAHLSGMLAAELGQDVALA
CCCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KRAGLLHDIGKAIDHEVEGSHVEIGVDLAKKYREHPTVINAIHSHHGDVEPDNIISILVA
HHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
AADALSAARPGARRETLENYVRRLNRLEEISESYEGVEKSFAIQAGREIRIIVTPEEVDD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
LKAHRIARDIKNQIEEELQYPGHIKVTVVREMRAVEYAK
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
SLTILLFILLSIIIGVAIGYFVAKNVLTNQQIQARTTADFIVAEANKEAESLKKEKLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHH
AKEEYQRRNDRLEEDYKERRSDLARQEARLLQKEENLDRKSDLLDKKDDLLEQKESKIEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHH
KQQHVDALEQSVQGIIAQHEQELTRISGLTQEEAKQEIIDQVEEELRHDIAVMVKEKEQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKQNVDKKAKMLLATTVQRLAAEHTSESTVSVINLPNDEMKGRIIGREGRNIRTLETLTG
HHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCCEECCCCCCCCHHHHHCC
VDLIIDDTPEAVILSGFDPIRREIAKTALESLVSDGRIHPGRIEEMVEKARKSVDEIIRE
CEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AGEAATFELGIHNLHPDIVKILGRMKYRTSYGQNVLKHSIEVAHLSGMLAAELGQDVALA
CCCCCEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KRAGLLHDIGKAIDHEVEGSHVEIGVDLAKKYREHPTVINAIHSHHGDVEPDNIISILVA
HHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
AADALSAARPGARRETLENYVRRLNRLEEISESYEGVEKSFAIQAGREIRIIVTPEEVDD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
LKAHRIARDIKNQIEEELQYPGHIKVTVVREMRAVEYAK
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA