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The map label for this gene is truB [H]

Identifier: 222151111

GI number: 222151111

Start: 946464

End: 947360

Strand: Direct

Name: truB [H]

Synonym: MCCL_0862

Alternate gene names: 222151111

Gene position: 946464-947360 (Clockwise)

Preceding gene: 222151110

Following gene: 222151112

Centisome position: 45.02

GC content: 44.59

Gene sequence:

>897_bases
ATGGATGGCATTATCGGTATTAATAAGGCACGTGGGATGACGAGTCACGATGTGGTGTTTAAGCTACGTAAAATATTAAG
GACGAAGAAAGTCGGCCACACCGGGACGCTTGATCCTGAAGTGGATGGGGTATTGCCGATTTGTGTCGGTAAGGCGACGC
GTATCAGTGATTATGTGATGCAGAGCGGTAAACGTTATATTGCAGAAGTGACCCTTGGCGTTCAGACGGCGACTGAAGAT
GCTCATGGTGAGATTGTGAAGACCGTATCGATTGAAGAGGGGATGTTCACAGAGGCGCAGGTTGATGAAGTATTACTTCG
ACTAACGGGTTCAATCAGACAAGTTCCACCGATGTATTCGGCGGTTAAGGTGAAAGGGCGCAAATTGTATGAATATGCAC
GTGAAGGAATTGAGGTTGAGCGTCCAGAACGTACAGTGCAGATTTACGAATTAATACGTACAAGTGCTGTTCGTTTTATG
GATGGTAAATGTATATTCAATATTGAAGTCGCATGTGGTAAAGGGACATATATTAGAACACTTGCTACTCAGATAGCAGA
AGGTCTGGGTACTATTGGCCATATGTCAGATTTGACACGAACGGAGAGCGGTGGATTTAAGCTTGAAGACTGTATTACGA
TAGATGCGTTACGTGAAGTACCCTATGATGCAATAGCTGAGCTTCTCAAACCGATAGAGACAGGGCTCGCCCATATGCGG
CATGTCGCTGTCGATGAGCCGACATCCGTCAAGATTATGTACGGCCAAAAGTTACGTCAGATGAGCCCACCGATTGAAGA
TGAGACGGTGATGACGTACAATGATAAGGTAATAGCAATTTTCATACCGGATGACAAGCATCCAGGTTTGATCAAAGCTA
AAAAAGTATTTAATTAG

Upstream 100 bases:

>100_bases
TAAAATCGAGCGTATGATTGCGGAATTAAATAGAGATAAATAGACCAATCACACTTTTTCATGTAAACTATTTTAGAGTT
TACTGAAGGAGTGTTTTTTT

Downstream 100 bases:

>100_bases
GGGATAGCTATGAAAACAATTGAAATGATACATCCGATCGAGCAGTGTTACGATCACGAACCTTGTGCGCTTGCAGTTGG
TTTCTTTGATGGGATTCATA

Product: tRNA pseudouridine 5S synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDGIIGINKARGMTSHDVVFKLRKILRTKKVGHTGTLDPEVDGVLPICVGKATRISDYVMQSGKRYIAEVTLGVQTATED
AHGEIVKTVSIEEGMFTEAQVDEVLLRLTGSIRQVPPMYSAVKVKGRKLYEYAREGIEVERPERTVQIYELIRTSAVRFM
DGKCIFNIEVACGKGTYIRTLATQIAEGLGTIGHMSDLTRTESGGFKLEDCITIDALREVPYDAIAELLKPIETGLAHMR
HVAVDEPTSVKIMYGQKLRQMSPPIEDETVMTYNDKVIAIFIPDDKHPGLIKAKKVFN

Sequences:

>Translated_298_residues
MDGIIGINKARGMTSHDVVFKLRKILRTKKVGHTGTLDPEVDGVLPICVGKATRISDYVMQSGKRYIAEVTLGVQTATED
AHGEIVKTVSIEEGMFTEAQVDEVLLRLTGSIRQVPPMYSAVKVKGRKLYEYAREGIEVERPERTVQIYELIRTSAVRFM
DGKCIFNIEVACGKGTYIRTLATQIAEGLGTIGHMSDLTRTESGGFKLEDCITIDALREVPYDAIAELLKPIETGLAHMR
HVAVDEPTSVKIMYGQKLRQMSPPIEDETVMTYNDKVIAIFIPDDKHPGLIKAKKVFN
>Mature_298_residues
MDGIIGINKARGMTSHDVVFKLRKILRTKKVGHTGTLDPEVDGVLPICVGKATRISDYVMQSGKRYIAEVTLGVQTATED
AHGEIVKTVSIEEGMFTEAQVDEVLLRLTGSIRQVPPMYSAVKVKGRKLYEYAREGIEVERPERTVQIYELIRTSAVRFM
DGKCIFNIEVACGKGTYIRTLATQIAEGLGTIGHMSDLTRTESGGFKLEDCITIDALREVPYDAIAELLKPIETGLAHMR
HVAVDEPTSVKIMYGQKLRQMSPPIEDETVMTYNDKVIAIFIPDDKHPGLIKAKKVFN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=227, Percent_Identity=32.15859030837, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI215599015, Length=267, Percent_Identity=31.0861423220974, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4503337, Length=267, Percent_Identity=31.0861423220974, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI2367200, Length=279, Percent_Identity=37.6344086021505, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17553978, Length=265, Percent_Identity=27.5471698113208, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=267, Percent_Identity=28.4644194756554, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324037, Length=134, Percent_Identity=39.5522388059701, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364189, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281364187, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281364185, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281364183, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI62471759, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17975520, Length=265, Percent_Identity=29.0566037735849, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33099; Mature: 33099

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGIIGINKARGMTSHDVVFKLRKILRTKKVGHTGTLDPEVDGVLPICVGKATRISDYVM
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHH
QSGKRYIAEVTLGVQTATEDAHGEIVKTVSIEEGMFTEAQVDEVLLRLTGSIRQVPPMYS
HCCCCEEEEEEECEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHH
AVKVKGRKLYEYAREGIEVERPERTVQIYELIRTSAVRFMDGKCIFNIEVACGKGTYIRT
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEECCEEEEEEEEEECCCHHHHH
LATQIAEGLGTIGHMSDLTRTESGGFKLEDCITIDALREVPYDAIAELLKPIETGLAHMR
HHHHHHHHHCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
HVAVDEPTSVKIMYGQKLRQMSPPIEDETVMTYNDKVIAIFIPDDKHPGLIKAKKVFN
HHCCCCCCEEEEEECHHHHHCCCCCCCCEEEEECCEEEEEEECCCCCCCCEEHHCCCC
>Mature Secondary Structure
MDGIIGINKARGMTSHDVVFKLRKILRTKKVGHTGTLDPEVDGVLPICVGKATRISDYVM
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHH
QSGKRYIAEVTLGVQTATEDAHGEIVKTVSIEEGMFTEAQVDEVLLRLTGSIRQVPPMYS
HCCCCEEEEEEECEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHH
AVKVKGRKLYEYAREGIEVERPERTVQIYELIRTSAVRFMDGKCIFNIEVACGKGTYIRT
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEECCEEEEEEEEEECCCHHHHH
LATQIAEGLGTIGHMSDLTRTESGGFKLEDCITIDALREVPYDAIAELLKPIETGLAHMR
HHHHHHHHHCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
HVAVDEPTSVKIMYGQKLRQMSPPIEDETVMTYNDKVIAIFIPDDKHPGLIKAKKVFN
HHCCCCCCEEEEEECHHHHHCCCCCCCCEEEEECCEEEEEEECCCCCCCCEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA