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The map label for this gene is gid [H]

Identifier: 222151084

GI number: 222151084

Start: 911697

End: 912998

Strand: Direct

Name: gid [H]

Synonym: MCCL_0835

Alternate gene names: 222151084

Gene position: 911697-912998 (Clockwise)

Preceding gene: 222151083

Following gene: 222151085

Centisome position: 43.37

GC content: 40.71

Gene sequence:

>1302_bases
ATGACAACTGTAAATATAATTGGTGCTGGTCTTGCCGGTAGTGAAGCGGCATACCAATTAGCAAAACGAGGTATTAAAGT
TAAACTATATGAAATGAGACCGGTAAAGCAGACCCCGGCTCATCATACAGACAAATTTGCTGAACTTGTCTGCTCAAATT
CATTACGAGGTAATGCGTTGACGAATGCTGTCGGTGTCCTTAAAGAAGAGATGCGCAGACTCGACTCATTGATTATTAAA
GCTGCTGATAACGCAAGTGTACCTGCAGGAGGAGCGTTAGCTGTAGATAGACATGAATTCAGTGGATATGTGACAGATAC
ATTAAAGCAACATGCAAATATTGAAGTTATCAATGAAGAAATTACAGAAATACCAGATGGACCGACGATTATTGCGACTG
GCCCATTAACTACTGAAAGCTTATCGAAACAGATTCAGGCGCTAACTGGACAAGATCATCTTTACTTCTATGATGCAGCT
GCACCTATCATTGAAAAGGATTCGATCGATATGGACAAAGTATATTTGAAGTCTCGTTATGATAAAGGCGAGGCTGCATA
TTTGAACTGTCCGATGACTGAAGAAGAGTTCAATACATTCTATGATGCTTTACTTGAAGCAGAAGTAGTGCCATTAAAGG
AATTCGAAAAAGAAATTTACTTTGAGGGCTGTATGCCGTTTGAAGAGATGGCAAAGCGAGGACGTAAAACGCTATTGTTT
GGACCGATGAAGCCTGTGGGTCTAGAAGATCCTAAGACTGGGAAACGTCCGTACGCAGTGGTTCAGCTACGTCAAGACGA
TGCAGCAGGAACACTTTATAATATTGTAGGTTTTCAGACGCATTTGAAGTGGGGCGCACAAAAAGAAATATTAAAGCTGA
TTCCTGGTCTTGAAAACGTGGAAGTGGTCAGATATGGTGTTATGCACCGTAACACGTTTATCAATTCACCGACAAATCTA
CAGCAGACGTATCAATTTAAAGGCAATGACAGTCTATTTCTTGCAGGTCAGATGACAGGTGTAGAAGGTTATGTGGAAAG
TGCAGCAAGCGGATTAGTAGCAGGAATCAATATGGCTAAATACGTTGAAGATAAGCCGGTTATCACATTCCCTGAAAATA
CAGTGATCGGTTCAATGGCATATTATATTACGCATACGAACAGCAAGAACTTTCAGCCGATGAATGCCAATTTCGGGCTC
TTACCAAGTCTGGATAAACGTATTAAAGATAAAAAAGAACGATATGAGGCGCTCGCAGCACGTGCCTTAGCCTCTTTAGA
ACATTTTAAAGTTACTTTATAG

Upstream 100 bases:

>100_bases
TTTTCGATTTTTGTGAAAAATGAATCTGTTTTTAATAATAATAGTTTATTTCATTACAAAATTGTAATACAATCATGAAG
GATATTCAGGAGGAGATAAT

Downstream 100 bases:

>100_bases
TGTTTTAGTTGTGTGTCAATTTTAAATTGTGATAGAATTCAGATAAATAGGAGGGCTGTATGGATACTAATGATTTGCAA
AGATTCTTAACTTACTTGAA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTTVNIIGAGLAGSEAAYQLAKRGIKVKLYEMRPVKQTPAHHTDKFAELVCSNSLRGNALTNAVGVLKEEMRRLDSLIIK
AADNASVPAGGALAVDRHEFSGYVTDTLKQHANIEVINEEITEIPDGPTIIATGPLTTESLSKQIQALTGQDHLYFYDAA
APIIEKDSIDMDKVYLKSRYDKGEAAYLNCPMTEEEFNTFYDALLEAEVVPLKEFEKEIYFEGCMPFEEMAKRGRKTLLF
GPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEILKLIPGLENVEVVRYGVMHRNTFINSPTNL
QQTYQFKGNDSLFLAGQMTGVEGYVESAASGLVAGINMAKYVEDKPVITFPENTVIGSMAYYITHTNSKNFQPMNANFGL
LPSLDKRIKDKKERYEALAARALASLEHFKVTL

Sequences:

>Translated_433_residues
MTTVNIIGAGLAGSEAAYQLAKRGIKVKLYEMRPVKQTPAHHTDKFAELVCSNSLRGNALTNAVGVLKEEMRRLDSLIIK
AADNASVPAGGALAVDRHEFSGYVTDTLKQHANIEVINEEITEIPDGPTIIATGPLTTESLSKQIQALTGQDHLYFYDAA
APIIEKDSIDMDKVYLKSRYDKGEAAYLNCPMTEEEFNTFYDALLEAEVVPLKEFEKEIYFEGCMPFEEMAKRGRKTLLF
GPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEILKLIPGLENVEVVRYGVMHRNTFINSPTNL
QQTYQFKGNDSLFLAGQMTGVEGYVESAASGLVAGINMAKYVEDKPVITFPENTVIGSMAYYITHTNSKNFQPMNANFGL
LPSLDKRIKDKKERYEALAARALASLEHFKVTL
>Mature_432_residues
TTVNIIGAGLAGSEAAYQLAKRGIKVKLYEMRPVKQTPAHHTDKFAELVCSNSLRGNALTNAVGVLKEEMRRLDSLIIKA
ADNASVPAGGALAVDRHEFSGYVTDTLKQHANIEVINEEITEIPDGPTIIATGPLTTESLSKQIQALTGQDHLYFYDAAA
PIIEKDSIDMDKVYLKSRYDKGEAAYLNCPMTEEEFNTFYDALLEAEVVPLKEFEKEIYFEGCMPFEEMAKRGRKTLLFG
PMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEILKLIPGLENVEVVRYGVMHRNTFINSPTNLQ
QTYQFKGNDSLFLAGQMTGVEGYVESAASGLVAGINMAKYVEDKPVITFPENTVIGSMAYYITHTNSKNFQPMNANFGLL
PSLDKRIKDKKERYEALAARALASLEHFKVTL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=89, Percent_Identity=39.3258426966292, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17534255, Length=90, Percent_Identity=40, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 48022; Mature: 47890

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVNIIGAGLAGSEAAYQLAKRGIKVKLYEMRPVKQTPAHHTDKFAELVCSNSLRGNAL
CCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHH
TNAVGVLKEEMRRLDSLIIKAADNASVPAGGALAVDRHEFSGYVTDTLKQHANIEVINEE
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECHHCCCHHHHHHHHCCCEEEECHH
ITEIPDGPTIIATGPLTTESLSKQIQALTGQDHLYFYDAAAPIIEKDSIDMDKVYLKSRY
HHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCC
DKGEAAYLNCPMTEEEFNTFYDALLEAEVVPLKEFEKEIYFEGCMPFEEMAKRGRKTLLF
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCEEEE
GPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEILKLIPGLENV
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
EVVRYGVMHRNTFINSPTNLQQTYQFKGNDSLFLAGQMTGVEGYVESAASGLVAGINMAK
HHHEEHHHHCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHH
YVEDKPVITFPENTVIGSMAYYITHTNSKNFQPMNANFGLLPSLDKRIKDKKERYEALAA
HHCCCCEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RALASLEHFKVTL
HHHHHHHHHEEEC
>Mature Secondary Structure 
TTVNIIGAGLAGSEAAYQLAKRGIKVKLYEMRPVKQTPAHHTDKFAELVCSNSLRGNAL
CEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHH
TNAVGVLKEEMRRLDSLIIKAADNASVPAGGALAVDRHEFSGYVTDTLKQHANIEVINEE
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECHHCCCHHHHHHHHCCCEEEECHH
ITEIPDGPTIIATGPLTTESLSKQIQALTGQDHLYFYDAAAPIIEKDSIDMDKVYLKSRY
HHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCC
DKGEAAYLNCPMTEEEFNTFYDALLEAEVVPLKEFEKEIYFEGCMPFEEMAKRGRKTLLF
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCEEEE
GPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEILKLIPGLENV
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
EVVRYGVMHRNTFINSPTNLQQTYQFKGNDSLFLAGQMTGVEGYVESAASGLVAGINMAK
HHHEEHHHHCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHH
YVEDKPVITFPENTVIGSMAYYITHTNSKNFQPMNANFGLLPSLDKRIKDKKERYEALAA
HHCCCCEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RALASLEHFKVTL
HHHHHHHHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA