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The map label for this gene is topA [H]

Identifier: 222151083

GI number: 222151083

Start: 909469

End: 911532

Strand: Direct

Name: topA [H]

Synonym: MCCL_0834

Alternate gene names: 222151083

Gene position: 909469-911532 (Clockwise)

Preceding gene: 222151082

Following gene: 222151084

Centisome position: 43.26

GC content: 39.29

Gene sequence:

>2064_bases
TTGGCAGAAAATCTCGTCATTGTTGAGTCGCCTGCAAAAGCTAAAACCATTGAAAAATATTTAGGGAAGAAATTTAAAGT
TGTCGCATCTATGGGGCATGTACGTGATCTACCACGTAGCCAGATGGGCGTTGATACTGAAAATGATTTTGAACCAAGAT
ATATAACGATTAGAGGAAAAGGGCCTATCGTTCAAGATTTAAAAAAACATGCAAAGCGCGCGAAGCATGTCTATCTTGCA
AGTGACCCGGACCGTGAAGGTGAAGCGATTGCCTGGCATTTGGCACATATACTGAATATAGATGAAAAAGCAGCGTCCAG
AGTTGTATTCAATGAAATTACAAAGGATGCTGTCAAAGAGAGTTTTAAGCATCCAAGACAAATTGAACAGAATCTTGTTG
ATGCACAGCAAGCACGACGCGTACTTGACCGTCTCGTAGGCTATAACATTTCTCCGGTATTGTGGAAGAAAGTTAAAAAG
GGGCTTTCGGCTGGCCGTGTTCAATCTGTAGCACTTAAGCTTATTATTGATCGAGAAGAAGAAATCAATGCATTTAAACC
AGAGGAGTACTGGACGATTGATGGATTGTTTGAATATGACAGAAAACAGTTCAAGGCAAAGTTCTTACATGAGAATAATA
AGCCTAAGAAATTAAAACAAGAAGACGATGTTAAAGCGATTGTCAAACAACTGAAATCAGATGCATTTGAAGTAACGGAT
GTGACTAAAAAAGAAAAGCTCAGAAATCCTGCACAACCATTTACGACGAGTTCACTCCAGCAGGAAGCTGCTCGTAAGCT
AAACTTTAAAGCCCGTAAGACGATGATGCTTGCTCAGCAATTATATGAAGGAATAGACTTGAAGAAACAAGGTGCAGTGG
GTTTAATTACTTATATGCGTACAGATTCTACTCGTATTTCAGATACTGCAAAGAACGAGGCTGCGAGCTATATTGAAGAA
CATTATGGTAAAGCATACTTGTCTAAACGCAGTAGTAAGAGTGGCGGTGCTCAAGATGCACATGAAGCAGTACGTCCAAC
ATCGGTGATGCGTACGCCTTCTGAGATGAAAGCTTATCTTTCTAGAGATCAGCTACGTCTATACAAATTGATCTGGGAGC
GTTTTATGGCAAGTCAAATGGCTGCAGCAGTCATTGATACGGTCAGTGTAGACCTTGTTCAGAATAATATTAAATTCAGA
GCGAATGGTCAGACGATTAAGTTTAAAGGATTTATGAGTGTGTACATCGAAGGTCAGGATGAAGAAACTGAAGATGGAGA
GAATAAATTACCGGTGCTGGAAGTGGGTGATACAGTTAAGGCTGTTAATATCGATGAAAACCAACATTTCACGCAACCAC
CTCCGCGCTATACTGAAGCCCGTCTTGTTAAAACGATGGAAGAAAAAGGTATTGGTCGACCTTCAACGTATGCACCAACT
ATAGATACGATCCAAAAGCGTAATTACGTAAAGGTAGACCAGAAGAAATTTTATCCGACAGAGCTTGGAATCATTGTCAA
CGAGTCTGTTAAAGAATATTTCCCTGAAATTATTGATGTTGATTTCACGGTGAATATGGAAACATTATTAGACAAAATTG
CCAGTGGAGATATGGAATGGAAGAAGGTCATTTCAACTTTTTATGGTGATTTCAAAACACATGTTGAACGTGCGGAAGCA
GAAATGGAAAAGATTGAAATCAAAGACGAACCAGCAGGAGAAGATTGTGAGAAATGCGGTTCTCCGATGGTATATAAAAT
GGGACGTTACGGTAAATTTATGGCCTGTTCAAATTTCCCTGAATGCAGAAATACAAAAGCAATCGTCAAAACTATTGGTG
TAGCCTGTCCAAAGTGTAAAGATGGCGAAGTTGTTGAACGTAAATCTAAGAAAAATAGAATTTTTTATGGCTGTAGCAAT
TTTCCGGAATGTGATTATATTTCCTGGGATAAACCTATTGCAAGAAATTGTCCGAAGTGTGACACACAGCTTGTGGAACG
TAAAAAAGGAAAGCAGACACAAGTTGTATGTACGAATTGTGATTACGATGAGAAACCTCAATAG

Upstream 100 bases:

>100_bases
AAAAAATTAAAACTATATTTATGAGTTTTATTTGACAATCACTTACTTATACGTATATTATTTATCATTGAAAACAATTA
AGTAAGAGGGGGATATACAA

Downstream 100 bases:

>100_bases
CACATCCATAAATAATCATGTTAATCTGAGAACAGATTTGAAATATAATCTGTTCTTTATTATTTTTTCGATTTTTGTGA
AAAATGAATCTGTTTTTAAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MAENLVIVESPAKAKTIEKYLGKKFKVVASMGHVRDLPRSQMGVDTENDFEPRYITIRGKGPIVQDLKKHAKRAKHVYLA
SDPDREGEAIAWHLAHILNIDEKAASRVVFNEITKDAVKESFKHPRQIEQNLVDAQQARRVLDRLVGYNISPVLWKKVKK
GLSAGRVQSVALKLIIDREEEINAFKPEEYWTIDGLFEYDRKQFKAKFLHENNKPKKLKQEDDVKAIVKQLKSDAFEVTD
VTKKEKLRNPAQPFTTSSLQQEAARKLNFKARKTMMLAQQLYEGIDLKKQGAVGLITYMRTDSTRISDTAKNEAASYIEE
HYGKAYLSKRSSKSGGAQDAHEAVRPTSVMRTPSEMKAYLSRDQLRLYKLIWERFMASQMAAAVIDTVSVDLVQNNIKFR
ANGQTIKFKGFMSVYIEGQDEETEDGENKLPVLEVGDTVKAVNIDENQHFTQPPPRYTEARLVKTMEEKGIGRPSTYAPT
IDTIQKRNYVKVDQKKFYPTELGIIVNESVKEYFPEIIDVDFTVNMETLLDKIASGDMEWKKVISTFYGDFKTHVERAEA
EMEKIEIKDEPAGEDCEKCGSPMVYKMGRYGKFMACSNFPECRNTKAIVKTIGVACPKCKDGEVVERKSKKNRIFYGCSN
FPECDYISWDKPIARNCPKCDTQLVERKKGKQTQVVCTNCDYDEKPQ

Sequences:

>Translated_687_residues
MAENLVIVESPAKAKTIEKYLGKKFKVVASMGHVRDLPRSQMGVDTENDFEPRYITIRGKGPIVQDLKKHAKRAKHVYLA
SDPDREGEAIAWHLAHILNIDEKAASRVVFNEITKDAVKESFKHPRQIEQNLVDAQQARRVLDRLVGYNISPVLWKKVKK
GLSAGRVQSVALKLIIDREEEINAFKPEEYWTIDGLFEYDRKQFKAKFLHENNKPKKLKQEDDVKAIVKQLKSDAFEVTD
VTKKEKLRNPAQPFTTSSLQQEAARKLNFKARKTMMLAQQLYEGIDLKKQGAVGLITYMRTDSTRISDTAKNEAASYIEE
HYGKAYLSKRSSKSGGAQDAHEAVRPTSVMRTPSEMKAYLSRDQLRLYKLIWERFMASQMAAAVIDTVSVDLVQNNIKFR
ANGQTIKFKGFMSVYIEGQDEETEDGENKLPVLEVGDTVKAVNIDENQHFTQPPPRYTEARLVKTMEEKGIGRPSTYAPT
IDTIQKRNYVKVDQKKFYPTELGIIVNESVKEYFPEIIDVDFTVNMETLLDKIASGDMEWKKVISTFYGDFKTHVERAEA
EMEKIEIKDEPAGEDCEKCGSPMVYKMGRYGKFMACSNFPECRNTKAIVKTIGVACPKCKDGEVVERKSKKNRIFYGCSN
FPECDYISWDKPIARNCPKCDTQLVERKKGKQTQVVCTNCDYDEKPQ
>Mature_686_residues
AENLVIVESPAKAKTIEKYLGKKFKVVASMGHVRDLPRSQMGVDTENDFEPRYITIRGKGPIVQDLKKHAKRAKHVYLAS
DPDREGEAIAWHLAHILNIDEKAASRVVFNEITKDAVKESFKHPRQIEQNLVDAQQARRVLDRLVGYNISPVLWKKVKKG
LSAGRVQSVALKLIIDREEEINAFKPEEYWTIDGLFEYDRKQFKAKFLHENNKPKKLKQEDDVKAIVKQLKSDAFEVTDV
TKKEKLRNPAQPFTTSSLQQEAARKLNFKARKTMMLAQQLYEGIDLKKQGAVGLITYMRTDSTRISDTAKNEAASYIEEH
YGKAYLSKRSSKSGGAQDAHEAVRPTSVMRTPSEMKAYLSRDQLRLYKLIWERFMASQMAAAVIDTVSVDLVQNNIKFRA
NGQTIKFKGFMSVYIEGQDEETEDGENKLPVLEVGDTVKAVNIDENQHFTQPPPRYTEARLVKTMEEKGIGRPSTYAPTI
DTIQKRNYVKVDQKKFYPTELGIIVNESVKEYFPEIIDVDFTVNMETLLDKIASGDMEWKKVISTFYGDFKTHVERAEAE
MEKIEIKDEPAGEDCEKCGSPMVYKMGRYGKFMACSNFPECRNTKAIVKTIGVACPKCKDGEVVERKSKKNRIFYGCSNF
PECDYISWDKPIARNCPKCDTQLVERKKGKQTQVVCTNCDYDEKPQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=637, Percent_Identity=25.5886970172684, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI4507635, Length=650, Percent_Identity=24, Blast_Score=119, Evalue=9e-27,
Organism=Escherichia coli, GI1787529, Length=753, Percent_Identity=37.5830013280212, Blast_Score=500, Evalue=1e-142,
Organism=Escherichia coli, GI1788061, Length=537, Percent_Identity=21.7877094972067, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI48994931, Length=102, Percent_Identity=39.2156862745098, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=655, Percent_Identity=25.4961832061069, Blast_Score=133, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=515, Percent_Identity=24.4660194174757, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=499, Percent_Identity=23.8476953907816, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585251, Length=628, Percent_Identity=24.8407643312102, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24640096, Length=557, Percent_Identity=22.8007181328546, Blast_Score=112, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78489; Mature: 78358

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENLVIVESPAKAKTIEKYLGKKFKVVASMGHVRDLPRSQMGVDTENDFEPRYITIRGK
CCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPIVQDLKKHAKRAKHVYLASDPDREGEAIAWHLAHILNIDEKAASRVVFNEITKDAVKE
CCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SFKHPRQIEQNLVDAQQARRVLDRLVGYNISPVLWKKVKKGLSAGRVQSVALKLIIDREE
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHEECCHH
EINAFKPEEYWTIDGLFEYDRKQFKAKFLHENNKPKKLKQEDDVKAIVKQLKSDAFEVTD
HCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEHHH
VTKKEKLRNPAQPFTTSSLQQEAARKLNFKARKTMMLAQQLYEGIDLKKQGAVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
TDSTRISDTAKNEAASYIEEHYGKAYLSKRSSKSGGAQDAHEAVRPTSVMRTPSEMKAYL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCHHHHHHHH
SRDQLRLYKLIWERFMASQMAAAVIDTVSVDLVQNNIKFRANGQTIKFKGFMSVYIEGQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEEEEEEECCC
EETEDGENKLPVLEVGDTVKAVNIDENQHFTQPPPRYTEARLVKTMEEKGIGRPSTYAPT
CCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
IDTIQKRNYVKVDQKKFYPTELGIIVNESVKEYFPEIIDVDFTVNMETLLDKIASGDMEW
HHHHHCCCCEEECCCCCCCCHHCEEECHHHHHHCHHHCCCCEEECHHHHHHHHHCCCHHH
KKVISTFYGDFKTHVERAEAEMEKIEIKDEPAGEDCEKCGSPMVYKMGRYGKFMACSNFP
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCEECCCCCCCEEEECCCC
ECRNTKAIVKTIGVACPKCKDGEVVERKSKKNRIFYGCSNFPECDYISWDKPIARNCPKC
CCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHCCCCCH
DTQLVERKKGKQTQVVCTNCDYDEKPQ
HHHHHHHHCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
AENLVIVESPAKAKTIEKYLGKKFKVVASMGHVRDLPRSQMGVDTENDFEPRYITIRGK
CCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPIVQDLKKHAKRAKHVYLASDPDREGEAIAWHLAHILNIDEKAASRVVFNEITKDAVKE
CCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SFKHPRQIEQNLVDAQQARRVLDRLVGYNISPVLWKKVKKGLSAGRVQSVALKLIIDREE
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHEECCHH
EINAFKPEEYWTIDGLFEYDRKQFKAKFLHENNKPKKLKQEDDVKAIVKQLKSDAFEVTD
HCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEHHH
VTKKEKLRNPAQPFTTSSLQQEAARKLNFKARKTMMLAQQLYEGIDLKKQGAVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
TDSTRISDTAKNEAASYIEEHYGKAYLSKRSSKSGGAQDAHEAVRPTSVMRTPSEMKAYL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCHHHHHHHH
SRDQLRLYKLIWERFMASQMAAAVIDTVSVDLVQNNIKFRANGQTIKFKGFMSVYIEGQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEEEEEEECCC
EETEDGENKLPVLEVGDTVKAVNIDENQHFTQPPPRYTEARLVKTMEEKGIGRPSTYAPT
CCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
IDTIQKRNYVKVDQKKFYPTELGIIVNESVKEYFPEIIDVDFTVNMETLLDKIASGDMEW
HHHHHCCCCEEECCCCCCCCHHCEEECHHHHHHCHHHCCCCEEECHHHHHHHHHCCCHHH
KKVISTFYGDFKTHVERAEAEMEKIEIKDEPAGEDCEKCGSPMVYKMGRYGKFMACSNFP
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCEECCCCCCCEEEECCCC
ECRNTKAIVKTIGVACPKCKDGEVVERKSKKNRIFYGCSNFPECDYISWDKPIARNCPKC
CCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHCCCCCH
DTQLVERKKGKQTQVVCTNCDYDEKPQ
HHHHHHHHCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA