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The map label for this gene is rlmN [H]

Identifier: 222151049

GI number: 222151049

Start: 868661

End: 869752

Strand: Direct

Name: rlmN [H]

Synonym: MCCL_0800

Alternate gene names: 222151049

Gene position: 868661-869752 (Clockwise)

Preceding gene: 222151048

Following gene: 222151050

Centisome position: 41.32

GC content: 38.0

Gene sequence:

>1092_bases
ATGGCATTATTAGAAAAGAAAAATAAAAAGTTCATGCCGAACTTTGATAAACCCTCAATCTATTCATTACAACTCGGAGA
ATTAAAAGAATGGCTGGCAACGCATGGTCAGCAAAGTTTTAGAGCGAAACAGATTTACGACTGGTTATACGTAAAACGTG
TTAACTCATTTGAAGAGATGTCAAATTTATCGAAAGAACTGCGTAAAGTGTTAGAGGATAACTTTACGATGACAACGTTA
AAAACTGTTGTTAAGCAGGAAAGCAAGGACGGGACAATTAAATTTCTGTTTGAATTACAGGATGGATATACGATTGAAAC
AGTGTTAATGCGTCATGATTATGGCAATTCAGTCTGTGTAACAACGCAAGTAGGCTGCCGTATCGGTTGTACGTTCTGTG
CATCAACGTTAGGTGGTTTGAAACGAAATCTAGAAGCTGGCGAAATTGTTGCACAAGTATTAAATGTTCAAAAAGCATTA
GACGAAGTAGAGGAACGAGTAAGCCATGTCGTTATTATGGGTATTGGTGAACCATTTGAAAACTATGAAGAGATGATGGA
TTTCTTAAAGGTTATTAATCATGATGACGGTCTTAATATCGGTGCACGTCATATTACAGTATCTACATCTGGCATCATCC
CACGTATCTATGACTTTGCTGATGAAGAACTGCAGATTAACTTTGCACTTAGTCTACATGGACCGAATAACGATATTAGA
AGTCGCTTAATGCCGATCAATCGCGCTTATGATCTAGAGAAGTTGATGGAGTCAATCGAATACTATGTCAATAAAACAGG
ACGACGTATTACTTTTGAATATGGATTATTCGGTGGCGTAAATGATCAGGTTCATCACGCTAAAGAATTAGCCCAGCTGA
TCAAACACCTCAACTGCCATGTCAATTTGATCCCTGTAAATCATGTGCCTGAACGTGACTATGTTCGTACGCCGAAAGAA
GATATCTTTAAGTTTGAAAAAGAATTAAAACGCAATGGAATCAATGCTACAATTAGACGTGAACACGGTTCTGATATTGA
CGCAGCTTGTGGTCAGTTAAGAGCGAAAGAGAGACAAGAAGAAACGAGGTAG

Upstream 100 bases:

>100_bases
AACTACCGGTTATCGGTAAGCAAAAATTATTGCAGGTGTTGCCGCAAGACTTTAATTCTGACGGCTTCTTCATTGCAAAA
CTAAGAAGGAAGTGTAAATA

Downstream 100 bases:

>100_bases
AGATATGCATGCACGCTTTTTTACCGATATCGGTCCAGTAAGAGCAAGAAATGAAGATGCAGGTGGCGTTTATTATAATC
ATACAGGTCAGATGCTAATG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MALLEKKNKKFMPNFDKPSIYSLQLGELKEWLATHGQQSFRAKQIYDWLYVKRVNSFEEMSNLSKELRKVLEDNFTMTTL
KTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVLNVQKAL
DEVEERVSHVVIMGIGEPFENYEEMMDFLKVINHDDGLNIGARHITVSTSGIIPRIYDFADEELQINFALSLHGPNNDIR
SRLMPINRAYDLEKLMESIEYYVNKTGRRITFEYGLFGGVNDQVHHAKELAQLIKHLNCHVNLIPVNHVPERDYVRTPKE
DIFKFEKELKRNGINATIRREHGSDIDAACGQLRAKERQEETR

Sequences:

>Translated_363_residues
MALLEKKNKKFMPNFDKPSIYSLQLGELKEWLATHGQQSFRAKQIYDWLYVKRVNSFEEMSNLSKELRKVLEDNFTMTTL
KTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVLNVQKAL
DEVEERVSHVVIMGIGEPFENYEEMMDFLKVINHDDGLNIGARHITVSTSGIIPRIYDFADEELQINFALSLHGPNNDIR
SRLMPINRAYDLEKLMESIEYYVNKTGRRITFEYGLFGGVNDQVHHAKELAQLIKHLNCHVNLIPVNHVPERDYVRTPKE
DIFKFEKELKRNGINATIRREHGSDIDAACGQLRAKERQEETR
>Mature_362_residues
ALLEKKNKKFMPNFDKPSIYSLQLGELKEWLATHGQQSFRAKQIYDWLYVKRVNSFEEMSNLSKELRKVLEDNFTMTTLK
TVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVLNVQKALD
EVEERVSHVVIMGIGEPFENYEEMMDFLKVINHDDGLNIGARHITVSTSGIIPRIYDFADEELQINFALSLHGPNNDIRS
RLMPINRAYDLEKLMESIEYYVNKTGRRITFEYGLFGGVNDQVHHAKELAQLIKHLNCHVNLIPVNHVPERDYVRTPKED
IFKFEKELKRNGINATIRREHGSDIDAACGQLRAKERQEETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=34.593023255814, Blast_Score=235, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41793; Mature: 41662

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLEKKNKKFMPNFDKPSIYSLQLGELKEWLATHGQQSFRAKQIYDWLYVKRVNSFEEM
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
SNLSKELRKVLEDNFTMTTLKTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCV
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCEEEE
TTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVLNVQKALDEVEERVSHVVIMGIGEPFE
EEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
NYEEMMDFLKVINHDDGLNIGARHITVSTSGIIPRIYDFADEELQINFALSLHGPNNDIR
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEEEEEECCCHHHH
SRLMPINRAYDLEKLMESIEYYVNKTGRRITFEYGLFGGVNDQVHHAKELAQLIKHLNCH
HHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCE
VNLIPVNHVPERDYVRTPKEDIFKFEKELKRNGINATIRREHGSDIDAACGQLRAKERQE
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHH
ETR
CCC
>Mature Secondary Structure 
ALLEKKNKKFMPNFDKPSIYSLQLGELKEWLATHGQQSFRAKQIYDWLYVKRVNSFEEM
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
SNLSKELRKVLEDNFTMTTLKTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCV
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCEEEE
TTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVLNVQKALDEVEERVSHVVIMGIGEPFE
EEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
NYEEMMDFLKVINHDDGLNIGARHITVSTSGIIPRIYDFADEELQINFALSLHGPNNDIR
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEEEEEECCCHHHH
SRLMPINRAYDLEKLMESIEYYVNKTGRRITFEYGLFGGVNDQVHHAKELAQLIKHLNCH
HHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCE
VNLIPVNHVPERDYVRTPKEDIFKFEKELKRNGINATIRREHGSDIDAACGQLRAKERQE
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHH
ETR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA