Click here to switch to the map view.

The map label for this gene is def [C]

Identifier: 222151046

GI number: 222151046

Start: 865956

End: 866366

Strand: Direct

Name: def [C]

Synonym: MCCL_0797

Alternate gene names: 222151046

Gene position: 865956-866366 (Clockwise)

Preceding gene: 222151045

Following gene: 222151047

Centisome position: 41.19

GC content: 38.44

Gene sequence:

>411_bases
GTGATTAAACAATTAATAGAAGAACAAAACTCATTGTTACATCGTGAAGTTAAGGATGTAACACAGTTTGATGCGAGTTT
AAAGGCATTGATCAAAGATTTAGAAGATACATTATTTCATCATAATGGCGTTGGGATTGCTGCACCTCAGATTGGGGTCG
ATTTAAAGGTTGCTTTAGTGGATATGGAAGCTGATGGTATATTGCAACTTGTGAACCCGAAGATTGTTTCTTATTCAGAA
GAGACAGAAAGCGATGTAGAAGGTTGTCTATCGATCCCTGGCGTATTCGGTCTTGTTGATCGTTCGATTGAAATTGTAAT
CGAAGCAAACGATCTAGATGGGAATAAGATTGAAATGACCGCGTATGATGATATTGCGCGGTATTATTCAGCAGGGGAGT
GGATCACTTAA

Upstream 100 bases:

>100_bases
AACTTTGTTTAAGGAGAGTTTTATCATTTATATCAATACGTGGTAATACAGTGCGTTTTCATGTAAAATAGAATGATTGA
AGATATAAAGGAGAAGTTAC

Downstream 100 bases:

>100_bases
TTACGGGGAACTGTTCACAGAAAAGATGACGAAACAGTTAACGGAGAAAGAACTGGAGGAGATGTACGGTGAGTAAGATT
ATATTTATGGGTACGCCTGA

Product: polypeptide deformylase

Products: formate; L-methionine

Alternate protein names: Polypeptide deformylase-like [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MIKQLIEEQNSLLHREVKDVTQFDASLKALIKDLEDTLFHHNGVGIAAPQIGVDLKVALVDMEADGILQLVNPKIVSYSE
ETESDVEGCLSIPGVFGLVDRSIEIVIEANDLDGNKIEMTAYDDIARYYSAGEWIT

Sequences:

>Translated_136_residues
MIKQLIEEQNSLLHREVKDVTQFDASLKALIKDLEDTLFHHNGVGIAAPQIGVDLKVALVDMEADGILQLVNPKIVSYSE
ETESDVEGCLSIPGVFGLVDRSIEIVIEANDLDGNKIEMTAYDDIARYYSAGEWIT
>Mature_136_residues
MIKQLIEEQNSLLHREVKDVTQFDASLKALIKDLEDTLFHHNGVGIAAPQIGVDLKVALVDMEADGILQLVNPKIVSYSE
ETESDVEGCLSIPGVFGLVDRSIEIVIEANDLDGNKIEMTAYDDIARYYSAGEWIT

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=111, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: 3.5.1.31

Molecular weight: Translated: 15034; Mature: 15034

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQLIEEQNSLLHREVKDVTQFDASLKALIKDLEDTLFHHNGVGIAAPQIGVDLKVALV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEE
DMEADGILQLVNPKIVSYSEETESDVEGCLSIPGVFGLVDRSIEIVIEANDLDGNKIEMT
EECCCCEEEECCCEEECCCCHHHHHHHHHHCCCCHHHHHCCCEEEEEEECCCCCCEEEEE
AYDDIARYYSAGEWIT
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIKQLIEEQNSLLHREVKDVTQFDASLKALIKDLEDTLFHHNGVGIAAPQIGVDLKVALV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEE
DMEADGILQLVNPKIVSYSEETESDVEGCLSIPGVFGLVDRSIEIVIEANDLDGNKIEMT
EECCCCEEEECCCEEECCCCHHHHHHHHHHCCCCHHHHHCCCEEEEEEECCCCCCEEEEE
AYDDIARYYSAGEWIT
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-formyl-L-methionine; H2O

Specific reaction: N-formyl-L-methionine + H2O = formate + L-methionine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA