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The map label for this gene is fmt

Identifier: 222151047

GI number: 222151047

Start: 866435

End: 867367

Strand: Direct

Name: fmt

Synonym: MCCL_0798

Alternate gene names: 222151047

Gene position: 866435-867367 (Clockwise)

Preceding gene: 222151046

Following gene: 222151048

Centisome position: 41.21

GC content: 44.91

Gene sequence:

>933_bases
GTGAGTAAGATTATATTTATGGGTACGCCTGACTTTTCAGCACCAATCTTAAAGGCATTACATGAAGCGCATGGTGTAAG
TCTCGTCATTACACAGCCGGATAAACCTGTCGGGCGTAAGCGCGTACTAACACCACCTCCCGTTAAGGTGATGGCAGAAA
GCCTAGGCATTGAAGTGTACCAGCCTGAATCGATGAAAAGTGATGAAGCGTTTGAGCGTGTGCATGCGCTCAGTCCGGAT
TTAATCGTTACTGCTGCTTTCGGTCAGATATTACCGGAACGCGTACTTGATATTCCACGTCTCGGCTGTATTAATGTACA
TGCATCGCTGTTACCGAAGTATCGTGGCGGTGCACCGATTCATAAAGCGATTATTAACGGTGAGAAGTATTCGGGTGTGA
CAATCATGTATATGGTTAAACGTCTAGATGCAGGAGATATGATCGACAGTGTGCAAGTACCGATTGAAATCAATGATACT
GTTGGAACACTTCATGATAAGTTAAGTGTAGCAGGTACAGATTTATTACTTGAAGTAATGCCAAGTGTACTGAGCGGTAC
GAACAACCGCACACCTCAGAATGATAGTGAAGCGACATTTGCCTCTAACGTCAGTCGCGAAGAAGAGTATGTCACTTTTG
ACCGCACAGCTCTTGAGGTACACAACCACATTCGAGGTTTAAGTCCATGGCCTGTTGCATTCGCGAACTTTGATGGCAAA
GCGATGAAACTTTGGGCTTCTGAAATAGCTGAAGGTAGTGGTGCACCTGGTGAAATTATTCAGGCTGATAAGGCTGGTTT
AGTCATTGCTACAAATGATGGCGCTGTGCGCATTACTTCACTACAACCAGCTGGTAAGAAACGCATGGATGCTGCAAGTT
TTGTAGCGGGTGCGAAGTCACAACTCGTTGGGATGAAATTTAATGAAGCATAA

Upstream 100 bases:

>100_bases
GGTATTATTCAGCAGGGGAGTGGATCACTTAATTACGGGGAACTGTTCACAGAAAAGATGACGAAACAGTTAACGGAGAA
AGAACTGGAGGAGATGTACG

Downstream 100 bases:

>100_bases
TGTGAGAAGTGTTGCACTAGAAATATTAGATGCAGTTTTAACTGAAGGTGCATATAGTAATCTACTGATTAATGAAGCGA
TAAAAAAAGGTTACGTTGAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSKIIFMGTPDFSAPILKALHEAHGVSLVITQPDKPVGRKRVLTPPPVKVMAESLGIEVYQPESMKSDEAFERVHALSPD
LIVTAAFGQILPERVLDIPRLGCINVHASLLPKYRGGAPIHKAIINGEKYSGVTIMYMVKRLDAGDMIDSVQVPIEINDT
VGTLHDKLSVAGTDLLLEVMPSVLSGTNNRTPQNDSEATFASNVSREEEYVTFDRTALEVHNHIRGLSPWPVAFANFDGK
AMKLWASEIAEGSGAPGEIIQADKAGLVIATNDGAVRITSLQPAGKKRMDAASFVAGAKSQLVGMKFNEA

Sequences:

>Translated_310_residues
MSKIIFMGTPDFSAPILKALHEAHGVSLVITQPDKPVGRKRVLTPPPVKVMAESLGIEVYQPESMKSDEAFERVHALSPD
LIVTAAFGQILPERVLDIPRLGCINVHASLLPKYRGGAPIHKAIINGEKYSGVTIMYMVKRLDAGDMIDSVQVPIEINDT
VGTLHDKLSVAGTDLLLEVMPSVLSGTNNRTPQNDSEATFASNVSREEEYVTFDRTALEVHNHIRGLSPWPVAFANFDGK
AMKLWASEIAEGSGAPGEIIQADKAGLVIATNDGAVRITSLQPAGKKRMDAASFVAGAKSQLVGMKFNEA
>Mature_309_residues
SKIIFMGTPDFSAPILKALHEAHGVSLVITQPDKPVGRKRVLTPPPVKVMAESLGIEVYQPESMKSDEAFERVHALSPDL
IVTAAFGQILPERVLDIPRLGCINVHASLLPKYRGGAPIHKAIINGEKYSGVTIMYMVKRLDAGDMIDSVQVPIEINDTV
GTLHDKLSVAGTDLLLEVMPSVLSGTNNRTPQNDSEATFASNVSREEEYVTFDRTALEVHNHIRGLSPWPVAFANFDGKA
MKLWASEIAEGSGAPGEIIQADKAGLVIATNDGAVRITSLQPAGKKRMDAASFVAGAKSQLVGMKFNEA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=324, Percent_Identity=26.8518518518519, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21614513, Length=322, Percent_Identity=26.7080745341615, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI164663775, Length=226, Percent_Identity=29.2035398230088, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1789683, Length=304, Percent_Identity=42.1052631578947, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788589, Length=284, Percent_Identity=32.0422535211268, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI133930964, Length=323, Percent_Identity=25.6965944272446, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319458, Length=275, Percent_Identity=25.8181818181818, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45550868, Length=315, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571984, Length=237, Percent_Identity=29.535864978903, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585660, Length=318, Percent_Identity=23.8993710691824, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_MACCJ (B9EB94)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560201.1
- GeneID:   7390541
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0798
- OMA:   IMQMDEG
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33363; Mature: 33232

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIFMGTPDFSAPILKALHEAHGVSLVITQPDKPVGRKRVLTPPPVKVMAESLGIEVY
CCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCEEEE
QPESMKSDEAFERVHALSPDLIVTAAFGQILPERVLDIPRLGCINVHASLLPKYRGGAPI
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCCCCH
HKAIINGEKYSGVTIMYMVKRLDAGDMIDSVQVPIEINDTVGTLHDKLSVAGTDLLLEVM
HHHHCCCCCCCCEEHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
PSVLSGTNNRTPQNDSEATFASNVSREEEYVTFDRTALEVHNHIRGLSPWPVAFANFDGK
HHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEECCCCH
AMKLWASEIAEGSGAPGEIIQADKAGLVIATNDGAVRITSLQPAGKKRMDAASFVAGAKS
HHHHHHHHHHCCCCCCCCEEECCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCHH
QLVGMKFNEA
HEECEEECCC
>Mature Secondary Structure 
SKIIFMGTPDFSAPILKALHEAHGVSLVITQPDKPVGRKRVLTPPPVKVMAESLGIEVY
CCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCEEEE
QPESMKSDEAFERVHALSPDLIVTAAFGQILPERVLDIPRLGCINVHASLLPKYRGGAPI
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCCCCH
HKAIINGEKYSGVTIMYMVKRLDAGDMIDSVQVPIEINDTVGTLHDKLSVAGTDLLLEVM
HHHHCCCCCCCCEEHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
PSVLSGTNNRTPQNDSEATFASNVSREEEYVTFDRTALEVHNHIRGLSPWPVAFANFDGK
HHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEECCCCH
AMKLWASEIAEGSGAPGEIIQADKAGLVIATNDGAVRITSLQPAGKKRMDAASFVAGAKS
HHHHHHHHHHCCCCCCCCEEECCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCHH
QLVGMKFNEA
HEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA