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The map label for this gene is priA [H]

Identifier: 222151045

GI number: 222151045

Start: 863422

End: 865803

Strand: Direct

Name: priA [H]

Synonym: MCCL_0796

Alternate gene names: 222151045

Gene position: 863422-865803 (Clockwise)

Preceding gene: 222151044

Following gene: 222151046

Centisome position: 41.07

GC content: 42.23

Gene sequence:

>2382_bases
GTGATTGCAAAAGTTATCGTAGACGTTAATAGTAAACGTGTTGATAAGTTATTTGATTATTATATTCCGGAACATCTAAA
AAGCGATATCGTACCAGGCCAGAGAGTTGTTGTGCCGTTTGGCCCACGAAAAATACAGGGTTATGTTATCACAGTGACGG
ATAATGCAGAATACGACAAGCTGAAACCTGTCATTGCGATTCAGGACTTTATACCGGAACTTACGCCTGAATTAATAGAG
TTAAGTGAGTGGATGAGCTATTCTCACGTCTCAAAACGTATTGCAGTGCTCGATGCGATGCTGCCAAATGCAGTAAAAGG
GAAATATAAAAAGATATTTCGCCTAATAGACGACGAAACACTGCCTCAAGAACTCACGCAATATTTTAAAGATGCAACCT
TAACACTTGAACGCGCGCAGCAGCTTGAAATTGTTTCGCTGCTCAAGCCATATATTGAATGTGGTGACATCGTTGAAGAT
ATCGAAGTCTCTCAAAAATACAAGAAAAAGACATTCGTCGGTGTGAAGCTTAATCACTCGAATGTAGATGATATCATTTC
ACAGCTAAAATCCGAAACGCAGATACGAGCAATCGAACTCATAAATGATGCAGGTGAACTGACATTGATCGAACTGAAAG
AAGAAGGCATATCAAACGCTGTAGTCAATAACTTAGTCCAAAAAGGATTTTTAGAAAAAATCAATATTGAAACGACGCGA
GATCCGTATGCGCATAAAGTGTTCTTTGAAGATAAAGAAAAGGCACTGCATGATGAACAGCAGAGTGCATATAACGCTGT
TATGGCAACTATTAATAAAGATCAATCAGAAACATTCCTGTTACACGGGATTACCGGAAGCGGTAAGACAGAGGTATATC
TTCAACTCATTAAAGAAGTGCTGGACAAAGGGCAGGATGCCATCGTGCTCGTACCTGAAATTGCGCTGACACCTCAGATG
GTAGATCGATTCAAGTCCCGCTTCGGTGATGAAGTGGCTGTACTGCACTCGGGTCTTTCTAACGGTGAACGCTATGATGA
GTGGCGTAAGATAAAGATGGGAGATGCACGCGTATCTGTCGGTGCAAGGTCCAGTATATTCGCACCATTTAAAAATATCG
GCATCATCATTATCGATGAAGAGCATGAAACAACATATAAGCAGGAAGACTATCCACGATACCATGCCCGCGAAATTGCG
GAATGGCGTGCACAGTATCATCAATGTCCACTCATACTCGGCAGCGCCACACCGTCACTGGAAAGTTATGCACGTGCAGA
AAAGGGTGTCTATACGAAGCTTGAAATGTTAACACGTGCGAACAAATCCGCAATGCCTGAGATGATCGTCTCGAATATGC
GAGACGAACTGATGAACGGAAACAGGTCGATGTTCTCTAGTGTACTGCATGATAAGATTCAGGAGAAGATTGATAAAGGA
GAACAAGTCGTGCTCTTCTTAAATAGAAGAGGTCATGCACAGTTTATGCTGTGCCGTGACTGCGGACACGTACCGATGTG
TCCGAACTGCGACATTAGCTTAACGTACCATAAATATACGGATGAACTGAAGTGTCATTATTGTGGATATAAGGAACCAC
CTGTTAATATATGTCCGAGCTGTGAAAGTGAACATATACGCGAGATGGGCGTCGGTACGCAGAAGGTTGAAGAGATCATC
TATCAGACATTCCAGAATGCAAAAGTCATCCGTATGGATAACGATACGACACGCAAGAAAGGTGCGCATGAACGGCTGCT
TGAACAGTTTAAAAACGGAGAAGCGAATATTTTGCTCGGCACACAGATGATTGCAAAAGGACTGGATTTCCCGAACATTA
CACTCGTTGGCGTACTGAATGCCGATACGATGCTTAACTTACCGGACTTCCGAGCAAGTGAGAGAACATTCCAGCTCATA
ACACAAGTCGCTGGACGCGCCGGACGAAGTGAAAAGACAGGCGAAGTCGTTATTCAGACATATAATCCTGAACACTACGC
AATACAGCATGCGGTGAATTATGACTATAAAGCTTTCTTTAAAGAAGAGATGGAATTCAGAAGACTCGGCAAGTATCCGC
CGTACTTCTATATGATCTATCTTAATATGCAGCACCATAATTTAAAGACCGTACTGACCGAAAGTAAGCATATACATGAT
ATATTAGTGCAGCACCTATCACCGAAAGCATATGTACTTGGACCCGCACCGAGTCCAATCGCCCGAATTAATAATCAGCA
CCGCTTCCAGATACTGATTAAGTTTAAATCAGAACCCGAACTGTTAAAGGCACTTAATTATCTCGATGACCGATATCATG
AACTGTTTGTGAAAGATAAGTTCTCGCTGCATATCGATATAAACCCGTATATGATGATGTAA

Upstream 100 bases:

>100_bases
CGATGTATTTCAAAGACGGAACGCACATCGATATTCCGAAACAGTCTAAGCTGAACATCGCACATGAAATATTATCATAC
GTAAAAGAGAAGGGATTTAA

Downstream 100 bases:

>100_bases
TCATTCGTCATCACACACAAAATATAGTAAAATAACACTAAGCACTCTCTTTAACTTTGTTTAAGGAGAGTTTTATCATT
TATATCAATACGTGGTAATA

Product: primosomal protein

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MIAKVIVDVNSKRVDKLFDYYIPEHLKSDIVPGQRVVVPFGPRKIQGYVITVTDNAEYDKLKPVIAIQDFIPELTPELIE
LSEWMSYSHVSKRIAVLDAMLPNAVKGKYKKIFRLIDDETLPQELTQYFKDATLTLERAQQLEIVSLLKPYIECGDIVED
IEVSQKYKKKTFVGVKLNHSNVDDIISQLKSETQIRAIELINDAGELTLIELKEEGISNAVVNNLVQKGFLEKINIETTR
DPYAHKVFFEDKEKALHDEQQSAYNAVMATINKDQSETFLLHGITGSGKTEVYLQLIKEVLDKGQDAIVLVPEIALTPQM
VDRFKSRFGDEVAVLHSGLSNGERYDEWRKIKMGDARVSVGARSSIFAPFKNIGIIIIDEEHETTYKQEDYPRYHAREIA
EWRAQYHQCPLILGSATPSLESYARAEKGVYTKLEMLTRANKSAMPEMIVSNMRDELMNGNRSMFSSVLHDKIQEKIDKG
EQVVLFLNRRGHAQFMLCRDCGHVPMCPNCDISLTYHKYTDELKCHYCGYKEPPVNICPSCESEHIREMGVGTQKVEEII
YQTFQNAKVIRMDNDTTRKKGAHERLLEQFKNGEANILLGTQMIAKGLDFPNITLVGVLNADTMLNLPDFRASERTFQLI
TQVAGRAGRSEKTGEVVIQTYNPEHYAIQHAVNYDYKAFFKEEMEFRRLGKYPPYFYMIYLNMQHHNLKTVLTESKHIHD
ILVQHLSPKAYVLGPAPSPIARINNQHRFQILIKFKSEPELLKALNYLDDRYHELFVKDKFSLHIDINPYMMM

Sequences:

>Translated_793_residues
MIAKVIVDVNSKRVDKLFDYYIPEHLKSDIVPGQRVVVPFGPRKIQGYVITVTDNAEYDKLKPVIAIQDFIPELTPELIE
LSEWMSYSHVSKRIAVLDAMLPNAVKGKYKKIFRLIDDETLPQELTQYFKDATLTLERAQQLEIVSLLKPYIECGDIVED
IEVSQKYKKKTFVGVKLNHSNVDDIISQLKSETQIRAIELINDAGELTLIELKEEGISNAVVNNLVQKGFLEKINIETTR
DPYAHKVFFEDKEKALHDEQQSAYNAVMATINKDQSETFLLHGITGSGKTEVYLQLIKEVLDKGQDAIVLVPEIALTPQM
VDRFKSRFGDEVAVLHSGLSNGERYDEWRKIKMGDARVSVGARSSIFAPFKNIGIIIIDEEHETTYKQEDYPRYHAREIA
EWRAQYHQCPLILGSATPSLESYARAEKGVYTKLEMLTRANKSAMPEMIVSNMRDELMNGNRSMFSSVLHDKIQEKIDKG
EQVVLFLNRRGHAQFMLCRDCGHVPMCPNCDISLTYHKYTDELKCHYCGYKEPPVNICPSCESEHIREMGVGTQKVEEII
YQTFQNAKVIRMDNDTTRKKGAHERLLEQFKNGEANILLGTQMIAKGLDFPNITLVGVLNADTMLNLPDFRASERTFQLI
TQVAGRAGRSEKTGEVVIQTYNPEHYAIQHAVNYDYKAFFKEEMEFRRLGKYPPYFYMIYLNMQHHNLKTVLTESKHIHD
ILVQHLSPKAYVLGPAPSPIARINNQHRFQILIKFKSEPELLKALNYLDDRYHELFVKDKFSLHIDINPYMMM
>Mature_793_residues
MIAKVIVDVNSKRVDKLFDYYIPEHLKSDIVPGQRVVVPFGPRKIQGYVITVTDNAEYDKLKPVIAIQDFIPELTPELIE
LSEWMSYSHVSKRIAVLDAMLPNAVKGKYKKIFRLIDDETLPQELTQYFKDATLTLERAQQLEIVSLLKPYIECGDIVED
IEVSQKYKKKTFVGVKLNHSNVDDIISQLKSETQIRAIELINDAGELTLIELKEEGISNAVVNNLVQKGFLEKINIETTR
DPYAHKVFFEDKEKALHDEQQSAYNAVMATINKDQSETFLLHGITGSGKTEVYLQLIKEVLDKGQDAIVLVPEIALTPQM
VDRFKSRFGDEVAVLHSGLSNGERYDEWRKIKMGDARVSVGARSSIFAPFKNIGIIIIDEEHETTYKQEDYPRYHAREIA
EWRAQYHQCPLILGSATPSLESYARAEKGVYTKLEMLTRANKSAMPEMIVSNMRDELMNGNRSMFSSVLHDKIQEKIDKG
EQVVLFLNRRGHAQFMLCRDCGHVPMCPNCDISLTYHKYTDELKCHYCGYKEPPVNICPSCESEHIREMGVGTQKVEEII
YQTFQNAKVIRMDNDTTRKKGAHERLLEQFKNGEANILLGTQMIAKGLDFPNITLVGVLNADTMLNLPDFRASERTFQLI
TQVAGRAGRSEKTGEVVIQTYNPEHYAIQHAVNYDYKAFFKEEMEFRRLGKYPPYFYMIYLNMQHHNLKTVLTESKHIHD
ILVQHLSPKAYVLGPAPSPIARINNQHRFQILIKFKSEPELLKALNYLDDRYHELFVKDKFSLHIDINPYMMM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=515, Percent_Identity=41.3592233009709, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 91161; Mature: 91161

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKVIVDVNSKRVDKLFDYYIPEHLKSDIVPGQRVVVPFGPRKIQGYVITVTDNAEYDK
CCEEEEEECCHHHHHHHHHHHCHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCHHH
LKPVIAIQDFIPELTPELIELSEWMSYSHVSKRIAVLDAMLPNAVKGKYKKIFRLIDDET
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
LPQELTQYFKDATLTLERAQQLEIVSLLKPYIECGDIVEDIEVSQKYKKKTFVGVKLNHS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
NVDDIISQLKSETQIRAIELINDAGELTLIELKEEGISNAVVNNLVQKGFLEKINIETTR
CHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHCCCCCCC
DPYAHKVFFEDKEKALHDEQQSAYNAVMATINKDQSETFLLHGITGSGKTEVYLQLIKEV
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
LDKGQDAIVLVPEIALTPQMVDRFKSRFGDEVAVLHSGLSNGERYDEWRKIKMGDARVSV
HHCCCCEEEEECCHHCCHHHHHHHHHHCCCCEEHHHCCCCCCHHHHHHHHEECCCCEEEC
GARSSIFAPFKNIGIIIIDEEHETTYKQEDYPRYHAREIAEWRAQYHQCPLILGSATPSL
CCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCH
ESYARAEKGVYTKLEMLTRANKSAMPEMIVSNMRDELMNGNRSMFSSVLHDKIQEKIDKG
HHHHHHHCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EQVVLFLNRRGHAQFMLCRDCGHVPMCPNCDISLTYHKYTDELKCHYCGYKEPPVNICPS
CEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCC
CESEHIREMGVGTQKVEEIIYQTFQNAKVIRMDNDTTRKKGAHERLLEQFKNGEANILLG
CCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCHHCCHHHHHHHHHCCCCCEEEEE
TQMIAKGLDFPNITLVGVLNADTMLNLPDFRASERTFQLITQVAGRAGRSEKTGEVVIQT
HHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
YNPEHYAIQHAVNYDYKAFFKEEMEFRRLGKYPPYFYMIYLNMQHHNLKTVLTESKHIHD
ECCCCEEEEHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHCCHHHHH
ILVQHLSPKAYVLGPAPSPIARINNQHRFQILIKFKSEPELLKALNYLDDRYHELFVKDK
HHHHHCCCCEEEECCCCCHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHEEEECC
FSLHIDINPYMMM
EEEEEECCCEEEC
>Mature Secondary Structure
MIAKVIVDVNSKRVDKLFDYYIPEHLKSDIVPGQRVVVPFGPRKIQGYVITVTDNAEYDK
CCEEEEEECCHHHHHHHHHHHCHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCCCHHH
LKPVIAIQDFIPELTPELIELSEWMSYSHVSKRIAVLDAMLPNAVKGKYKKIFRLIDDET
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
LPQELTQYFKDATLTLERAQQLEIVSLLKPYIECGDIVEDIEVSQKYKKKTFVGVKLNHS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
NVDDIISQLKSETQIRAIELINDAGELTLIELKEEGISNAVVNNLVQKGFLEKINIETTR
CHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHCCCCCCC
DPYAHKVFFEDKEKALHDEQQSAYNAVMATINKDQSETFLLHGITGSGKTEVYLQLIKEV
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
LDKGQDAIVLVPEIALTPQMVDRFKSRFGDEVAVLHSGLSNGERYDEWRKIKMGDARVSV
HHCCCCEEEEECCHHCCHHHHHHHHHHCCCCEEHHHCCCCCCHHHHHHHHEECCCCEEEC
GARSSIFAPFKNIGIIIIDEEHETTYKQEDYPRYHAREIAEWRAQYHQCPLILGSATPSL
CCCCCCCCHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCH
ESYARAEKGVYTKLEMLTRANKSAMPEMIVSNMRDELMNGNRSMFSSVLHDKIQEKIDKG
HHHHHHHCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EQVVLFLNRRGHAQFMLCRDCGHVPMCPNCDISLTYHKYTDELKCHYCGYKEPPVNICPS
CEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCC
CESEHIREMGVGTQKVEEIIYQTFQNAKVIRMDNDTTRKKGAHERLLEQFKNGEANILLG
CCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCHHCCHHHHHHHHHCCCCCEEEEE
TQMIAKGLDFPNITLVGVLNADTMLNLPDFRASERTFQLITQVAGRAGRSEKTGEVVIQT
HHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
YNPEHYAIQHAVNYDYKAFFKEEMEFRRLGKYPPYFYMIYLNMQHHNLKTVLTESKHIHD
ECCCCEEEEHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHCCHHHHH
ILVQHLSPKAYVLGPAPSPIARINNQHRFQILIKFKSEPELLKALNYLDDRYHELFVKDK
HHHHHCCCCEEEECCCCCHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHEEEECC
FSLHIDINPYMMM
EEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]