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The map label for this gene is ctaB

Identifier: 222150981

GI number: 222150981

Start: 792251

End: 793171

Strand: Direct

Name: ctaB

Synonym: MCCL_0731

Alternate gene names: 222150981

Gene position: 792251-793171 (Clockwise)

Preceding gene: 222150979

Following gene: 222150982

Centisome position: 37.68

GC content: 38.76

Gene sequence:

>921_bases
ATGCAGAATGAACACATTTTAAAAGGTGTTAAATCAGTTGAACGGCGTCTGACTTTTAAAGATGTAAAAGCGATTGTGAA
GCTTGGACTCGTACAAGGCAATTTAATACCGGCATTTGCAGGTGCGTTTATTGCAATTATGTTAAGCGGTCGTTCATTTT
TAAGTAGTATACCTGAACTTTTAACGATGCTTTTCGGCACTACACTAATTATGGCAGGAAGTTGTGCGCTGAATAACTTT
TACGACCAGGATATTGACAGTATTATGCCAAGTAAGCAAAATCGACCATCTGTAACCGGTAAAGTTTCTACAGCTTCAAT
CCTACAGCTCAGTCTTGTGCTTATGATCGTTGGAGAGATGCTTTTGTTCACGATTAACATCGAGACAGGGATCATTGGAT
TTCTTGGCATCTTTGGCTATGTTGTTTTATATTCGGTCTGGTCTAAACGACATCTTGTGTCCAACACAATAATAGGAAGT
TTTCCAGGTGCGATTCCACCGCTTGTTGGCTATGCAGCGATTGAACCATCTCTTAGCAGCACAGCGTGGATGCTTTTCGT
CATTATGTTTATATGGCAGCCTGCGCATTTCTACGCGCTTGCAATTAAACGTAGTGAAGAATATGCACTTGCTGGTATAC
CGATGCTACCTTCAGTTAAAGGGTTTAAACGTACAAGGCTTTCCATGCTGTTTTGGGTGATGCTGTTGTTACCGACACCA
TTTTTCATGCAGGAACTCGGCACGGTATTTATGGTACTTGCTTCGGTATTAAATCTAGGATGGCTGTTATTAGCGATTTC
TGGATTTAGATCAAATGTTAAAGAGAATAAATGGGCAATGACAATGTTTGTATATTCTTTAAACTATTTGATGATATTTT
TTGTTATGATAGTAGTAGTTACATTAATTCAAACGATTTGA

Upstream 100 bases:

>100_bases
GAAAAATGTCACAATTTAATTTCGAAAATGTATAGAAAATGTGACAGTTTTCATATATTATTGTTATTATATGCGTTAGT
ATTGTAAAAGGAGGGTGAAA

Downstream 100 bases:

>100_bases
TTATGAATGGTGAAAGAGGGGTTAGAATTTAATGAAGAACAATTTAAAAAATGTAAAGCTGATCGGTTTATTTTCATTAT
TGTTAATGACCTTAGCAGGT

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQNEHILKGVKSVERRLTFKDVKAIVKLGLVQGNLIPAFAGAFIAIMLSGRSFLSSIPELLTMLFGTTLIMAGSCALNNF
YDQDIDSIMPSKQNRPSVTGKVSTASILQLSLVLMIVGEMLLFTINIETGIIGFLGIFGYVVLYSVWSKRHLVSNTIIGS
FPGAIPPLVGYAAIEPSLSSTAWMLFVIMFIWQPAHFYALAIKRSEEYALAGIPMLPSVKGFKRTRLSMLFWVMLLLPTP
FFMQELGTVFMVLASVLNLGWLLLAISGFRSNVKENKWAMTMFVYSLNYLMIFFVMIVVVTLIQTI

Sequences:

>Translated_306_residues
MQNEHILKGVKSVERRLTFKDVKAIVKLGLVQGNLIPAFAGAFIAIMLSGRSFLSSIPELLTMLFGTTLIMAGSCALNNF
YDQDIDSIMPSKQNRPSVTGKVSTASILQLSLVLMIVGEMLLFTINIETGIIGFLGIFGYVVLYSVWSKRHLVSNTIIGS
FPGAIPPLVGYAAIEPSLSSTAWMLFVIMFIWQPAHFYALAIKRSEEYALAGIPMLPSVKGFKRTRLSMLFWVMLLLPTP
FFMQELGTVFMVLASVLNLGWLLLAISGFRSNVKENKWAMTMFVYSLNYLMIFFVMIVVVTLIQTI
>Mature_306_residues
MQNEHILKGVKSVERRLTFKDVKAIVKLGLVQGNLIPAFAGAFIAIMLSGRSFLSSIPELLTMLFGTTLIMAGSCALNNF
YDQDIDSIMPSKQNRPSVTGKVSTASILQLSLVLMIVGEMLLFTINIETGIIGFLGIFGYVVLYSVWSKRHLVSNTIIGS
FPGAIPPLVGYAAIEPSLSSTAWMLFVIMFIWQPAHFYALAIKRSEEYALAGIPMLPSVKGFKRTRLSMLFWVMLLLPTP
FFMQELGTVFMVLASVLNLGWLLLAISGFRSNVKENKWAMTMFVYSLNYLMIFFVMIVVVTLIQTI

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=160, Percent_Identity=33.125, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1786631, Length=276, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71998235, Length=155, Percent_Identity=30.9677419354839, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325085, Length=184, Percent_Identity=29.3478260869565, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921064, Length=152, Percent_Identity=34.2105263157895, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COXX_MACCJ (B9EB27)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560134.1
- GeneID:   7390525
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0731
- OMA:   VAVYYEL
- ProtClustDB:   PRK04375
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.- [C]

Molecular weight: Translated: 34035; Mature: 34035

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x218bf410)-; HASH(0x219a0be8)-; HASH(0x21b4bcbc)-; HASH(0x21481d48)-; HASH(0x21b179b4)-; HASH(0x2121f6ac)-; HASH(0x21b4becc)-; HASH(0x21b4bbd8)-; HASH(0x1c8c4dc4)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNEHILKGVKSVERRLTFKDVKAIVKLGLVQGNLIPAFAGAFIAIMLSGRSFLSSIPEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LTMLFGTTLIMAGSCALNNFYDQDIDSIMPSKQNRPSVTGKVSTASILQLSLVLMIVGEM
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLFTINIETGIIGFLGIFGYVVLYSVWSKRHLVSNTIIGSFPGAIPPLVGYAAIEPSLSS
HHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
TAWMLFVIMFIWQPAHFYALAIKRSEEYALAGIPMLPSVKGFKRTRLSMLFWVMLLLPTP
HHHHHHHHHHHHCCHHHHHEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCH
FFMQELGTVFMVLASVLNLGWLLLAISGFRSNVKENKWAMTMFVYSLNYLMIFFVMIVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TLIQTI
HHHHHH
>Mature Secondary Structure
MQNEHILKGVKSVERRLTFKDVKAIVKLGLVQGNLIPAFAGAFIAIMLSGRSFLSSIPEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LTMLFGTTLIMAGSCALNNFYDQDIDSIMPSKQNRPSVTGKVSTASILQLSLVLMIVGEM
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLFTINIETGIIGFLGIFGYVVLYSVWSKRHLVSNTIIGSFPGAIPPLVGYAAIEPSLSS
HHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
TAWMLFVIMFIWQPAHFYALAIKRSEEYALAGIPMLPSVKGFKRTRLSMLFWVMLLLPTP
HHHHHHHHHHHHCCHHHHHEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCH
FFMQELGTVFMVLASVLNLGWLLLAISGFRSNVKENKWAMTMFVYSLNYLMIFFVMIVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TLIQTI
HHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA