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The map label for this gene is puuR [H]

Identifier: 222150964

GI number: 222150964

Start: 771121

End: 771663

Strand: Direct

Name: puuR [H]

Synonym: MCCL_0714

Alternate gene names: 222150964

Gene position: 771121-771663 (Clockwise)

Preceding gene: 222150963

Following gene: 222150965

Centisome position: 36.68

GC content: 38.31

Gene sequence:

>543_bases
ATGGATATCGGAAAAAAGATAAAAGATTTAAGGCGCCAGAAGAATTTGACACAAGAAGAGCTTGGCGAACGCACAGATCT
CTCGAAAGGTTATATTTCACAGCTGGAACGCAATCTATGTTCCCCTTCAATGGAAACATTCTTTAATATTTTGGAGGTCC
TCGGCTCTAAACCAAAAGATTTCTTCAGTGAACAGCAATATGAGCAGCGCATTCATTATCCAAAGTCAGAACAGACGATC
TATGATGAGTATGATGATGGCTATATGATTAACTGGCTTGTTCCAGATTCTAATGAATTTGACATGGAACCGGTCATTAT
AACGATTGAACCCGGTAAGAAGTATAAAAAGTTTCTGCCAAGTATGTCAGATAGTTTTATCTATGTGTTACAGGGAGAAG
CAAGCATTCATATTGGTAGAAAAGATTATCATGCGCAATGTGGGGAATCGTTCTATTTTAAGGCGAACGAATCTCATCAA
TTATTTAATCGAACAGACAAAGTGGCACGTGTACTGATTGTGGCGACAGAGTCTTACTTATAG

Upstream 100 bases:

>100_bases
AGTAAATTTTTAATGAATATTGAAATGTATATCAATTTTTGATACATTTAATACATTATTAAACTATTTGTTTATAATTA
TTAAACTAAGGTGTGAATGT

Downstream 100 bases:

>100_bases
AGGTGAACAATGAAACAATTTATTCAGTTTAACCATGTGACAAAGGCATATGGAGATAATACGATTCTTAATGATATCAG
CTTTGAAATTGAAAAAGGTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MDIGKKIKDLRRQKNLTQEELGERTDLSKGYISQLERNLCSPSMETFFNILEVLGSKPKDFFSEQQYEQRIHYPKSEQTI
YDEYDDGYMINWLVPDSNEFDMEPVIITIEPGKKYKKFLPSMSDSFIYVLQGEASIHIGRKDYHAQCGESFYFKANESHQ
LFNRTDKVARVLIVATESYL

Sequences:

>Translated_180_residues
MDIGKKIKDLRRQKNLTQEELGERTDLSKGYISQLERNLCSPSMETFFNILEVLGSKPKDFFSEQQYEQRIHYPKSEQTI
YDEYDDGYMINWLVPDSNEFDMEPVIITIEPGKKYKKFLPSMSDSFIYVLQGEASIHIGRKDYHAQCGESFYFKANESHQ
LFNRTDKVARVLIVATESYL
>Mature_180_residues
MDIGKKIKDLRRQKNLTQEELGERTDLSKGYISQLERNLCSPSMETFFNILEVLGSKPKDFFSEQQYEQRIHYPKSEQTI
YDEYDDGYMINWLVPDSNEFDMEPVIITIEPGKKYKKFLPSMSDSFIYVLQGEASIHIGRKDYHAQCGESFYFKANESHQ
LFNRTDKVARVLIVATESYL

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=173, Percent_Identity=22.5433526011561, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 21135; Mature: 21135

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIGKKIKDLRRQKNLTQEELGERTDLSKGYISQLERNLCSPSMETFFNILEVLGSKPKD
CCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FFSEQQYEQRIHYPKSEQTIYDEYDDGYMINWLVPDSNEFDMEPVIITIEPGKKYKKFLP
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHCC
SMSDSFIYVLQGEASIHIGRKDYHAQCGESFYFKANESHQLFNRTDKVARVLIVATESYL
CCCCCEEEEEECCCEEEECCCHHHHHCCCCEEEECCCCHHHHHHHHHHHEEEEEECCCCC
>Mature Secondary Structure
MDIGKKIKDLRRQKNLTQEELGERTDLSKGYISQLERNLCSPSMETFFNILEVLGSKPKD
CCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FFSEQQYEQRIHYPKSEQTIYDEYDDGYMINWLVPDSNEFDMEPVIITIEPGKKYKKFLP
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHCC
SMSDSFIYVLQGEASIHIGRKDYHAQCGESFYFKANESHQLFNRTDKVARVLIVATESYL
CCCCCEEEEEECCCEEEECCCHHHHHCCCCEEEECCCCHHHHHHHHHHHEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]