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The map label for this gene is pdhD [H]

Identifier: 222150963

GI number: 222150963

Start: 769273

End: 770679

Strand: Direct

Name: pdhD [H]

Synonym: MCCL_0713

Alternate gene names: 222150963

Gene position: 769273-770679 (Clockwise)

Preceding gene: 222150962

Following gene: 222150964

Centisome position: 36.59

GC content: 39.37

Gene sequence:

>1407_bases
ATGGTAGTTGGAGATTTTCCTATTGAAACAGATACTATTGTAATTGGAGCAGGTCCTGGTGGATATGTTGCAGCAATTCG
TGCAGCACAATTAGGACAAAAAGTAACAATCGTAGAAAAAGGAAACTTAGGGGGCGTTTGTCTAAACGTAGGTTGTATTC
CTTCAAAAGCAATGCTTTCAGCTTCTCATAAATACGAAACAGCGAAACATTCTGAAGATTACGGAATTAAAGCTGAAAAC
GTAACTTTAGATTTCTCTAAAGTTCAAGAATTCAAAAACGGTGTTGTTGAACGTTTAACTGGTGGCGTTGGTTCTTTACT
TAAAGGCCGCAAAGTTGAAATCGTTCAAGGTGAAGCTTACTTCGTAGACCAAAACAACTTAAAAGTGATGACTGAAAAAG
CATCACAAACTTACACGTTTAAGAATGCGATTATCGCGACTGGATCTCGTCCAATCGAAATTCCAAACTTCAAATTCAAC
AAACGTGTGATCGATTCAACAGGTGCATTAGCACTTCCTGAAGTACCTAAACACTTAGTTGTTGTAGGTGGCGGATATAT
TGGTTCTGAGTTAGGAACAGCTTACGCGAACTTTGGATCTAAAGTTACAATTTTAGAAGGCGCTAAAGATATCTTAGGCG
GTTTCGAAAAACAAATGACACAGGTTGTTAAAAAAGGTCTTAAAGCTAAAGGTGTAGAAATCGTTACTGAAGCAATGGCG
AAATCAGCTGAAGAATCAGAAAACGGTGTTAAAGTTACTTATGAAGCTAAAGGCGAAACACATACAATCGAAGCGGACTA
CTGCTTAGTAACTGTAGGTCGTCGTCCAAATACTGATGAATTAGGTCTAGAAGGTCTAGGTATTAAAATGACTGATCGTG
GTATCATTGAAGTTGACGCTCAAAGCCGTACTTCTGTTGAGAACATCTACGCAATCGGTGATATCGTTCCTGGACCACCA
TTAGCACACAAAGCTTCTTATGAAGGTAAAGTTGCAGCTGAAGCTATCTCAGGTGAAAAATCAGAAGTAGATTACTTAGG
TATTCCTGCTGTATGTTTCACAGAACCAGAATTAGCGACAGTTGGTTATACTGAAGCTATGGCTAAAGAAGAAGGATTAG
ACTTTAAAGCTTCTAAATTCCCTTATGCAGCAAATGGTCGTGCATTATCGATCGGTGAAACTGACGGTTTCTTAAAACTG
ATCACACTTAAAGAAGATGGATTATTAATCGGTGCGCAAGTTGCCGGTGCAGGTGCATCTGATGTCATCGCGGAACTTGG
TTTAGCGATTGAAACAGGTATGACTGCAGAAGATATTGCATTAACAATCCACGCTCACCCAACATTAGGTGAAATTACGA
TGGAAGCAGCAGAAGTTGCAATGGGTCATCCAATTCACACAATGTAA

Upstream 100 bases:

>100_bases
GACCACCGTCAAATCGATGGCGCTACTGGACAAAATGCAATGAACCATATTAAACGCTTGTTAAATAATCCAGAATTATT
ATTAATGGAGGGGTAAACAG

Downstream 100 bases:

>100_bases
TAAACTTTACAATTTCAAAGTACTATTCATATTATATGAATAGTACTTTTTTTATGGAGGAATGTATATGAACTATAGTT
ATCCAATCGATACAGAATGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; Membrane-bound ribosome protein complex 50 kDa subunit [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKAMLSASHKYETAKHSEDYGIKAEN
VTLDFSKVQEFKNGVVERLTGGVGSLLKGRKVEIVQGEAYFVDQNNLKVMTEKASQTYTFKNAIIATGSRPIEIPNFKFN
KRVIDSTGALALPEVPKHLVVVGGGYIGSELGTAYANFGSKVTILEGAKDILGGFEKQMTQVVKKGLKAKGVEIVTEAMA
KSAEESENGVKVTYEAKGETHTIEADYCLVTVGRRPNTDELGLEGLGIKMTDRGIIEVDAQSRTSVENIYAIGDIVPGPP
LAHKASYEGKVAAEAISGEKSEVDYLGIPAVCFTEPELATVGYTEAMAKEEGLDFKASKFPYAANGRALSIGETDGFLKL
ITLKEDGLLIGAQVAGAGASDVIAELGLAIETGMTAEDIALTIHAHPTLGEITMEAAEVAMGHPIHTM

Sequences:

>Translated_468_residues
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKAMLSASHKYETAKHSEDYGIKAEN
VTLDFSKVQEFKNGVVERLTGGVGSLLKGRKVEIVQGEAYFVDQNNLKVMTEKASQTYTFKNAIIATGSRPIEIPNFKFN
KRVIDSTGALALPEVPKHLVVVGGGYIGSELGTAYANFGSKVTILEGAKDILGGFEKQMTQVVKKGLKAKGVEIVTEAMA
KSAEESENGVKVTYEAKGETHTIEADYCLVTVGRRPNTDELGLEGLGIKMTDRGIIEVDAQSRTSVENIYAIGDIVPGPP
LAHKASYEGKVAAEAISGEKSEVDYLGIPAVCFTEPELATVGYTEAMAKEEGLDFKASKFPYAANGRALSIGETDGFLKL
ITLKEDGLLIGAQVAGAGASDVIAELGLAIETGMTAEDIALTIHAHPTLGEITMEAAEVAMGHPIHTM
>Mature_468_residues
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKAMLSASHKYETAKHSEDYGIKAEN
VTLDFSKVQEFKNGVVERLTGGVGSLLKGRKVEIVQGEAYFVDQNNLKVMTEKASQTYTFKNAIIATGSRPIEIPNFKFN
KRVIDSTGALALPEVPKHLVVVGGGYIGSELGTAYANFGSKVTILEGAKDILGGFEKQMTQVVKKGLKAKGVEIVTEAMA
KSAEESENGVKVTYEAKGETHTIEADYCLVTVGRRPNTDELGLEGLGIKMTDRGIIEVDAQSRTSVENIYAIGDIVPGPP
LAHKASYEGKVAAEAISGEKSEVDYLGIPAVCFTEPELATVGYTEAMAKEEGLDFKASKFPYAANGRALSIGETDGFLKL
ITLKEDGLLIGAQVAGAGASDVIAELGLAIETGMTAEDIALTIHAHPTLGEITMEAAEVAMGHPIHTM

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential). Membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=42.2077922077922, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=30.5194805194805, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=29.1028446389497, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI33519430, Length=455, Percent_Identity=28.1318681318681, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI33519428, Length=455, Percent_Identity=28.1318681318681, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI33519426, Length=455, Percent_Identity=28.1318681318681, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI148277065, Length=455, Percent_Identity=28.1318681318681, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=29.9349240780911, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI148277071, Length=455, Percent_Identity=28.1318681318681, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI291045268, Length=449, Percent_Identity=27.6169265033408, Blast_Score=128, Evalue=9e-30,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=45.4148471615721, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=30.2961275626424, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87081717, Length=446, Percent_Identity=30.2690582959641, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082354, Length=457, Percent_Identity=28.6652078774617, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789065, Length=139, Percent_Identity=30.2158273381295, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=41.0480349344978, Blast_Score=330, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=30.3609341825902, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=31.0657596371882, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=31.0657596371882, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71982272, Length=494, Percent_Identity=27.5303643724696, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=195, Percent_Identity=29.7435897435897, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=39.2781316348195, Blast_Score=309, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=26.6666666666667, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=28.6021505376344, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=44.4444444444444, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=28.7234042553192, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24640553, Length=470, Percent_Identity=28.7234042553192, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=28.7234042553192, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=26.8498942917548, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49340; Mature: 49340

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKAMLS
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHH
ASHKYETAKHSEDYGIKAENVTLDFSKVQEFKNGVVERLTGGVGSLLKGRKVEIVQGEAY
CCCCCCCCCCCCCCCEEECEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCEE
FVDQNNLKVMTEKASQTYTFKNAIIATGSRPIEIPNFKFNKRVIDSTGALALPEVPKHLV
EEECCCEEEEEECCCCEEEECCEEEECCCCCEECCCCCCCCEEECCCCCEECCCCCCEEE
VVGGGYIGSELGTAYANFGSKVTILEGAKDILGGFEKQMTQVVKKGLKAKGVEIVTEAMA
EEECCEECHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KSAEESENGVKVTYEAKGETHTIEADYCLVTVGRRPNTDELGLEGLGIKMTDRGIIEVDA
HHHCCCCCCEEEEEECCCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEECCCEEEECC
QSRTSVENIYAIGDIVPGPPLAHKASYEGKVAAEAISGEKSEVDYLGIPAVCFTEPELAT
CCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEHHHCCCCCCCEEECCCEEEECCCCEEE
VGYTEAMAKEEGLDFKASKFPYAANGRALSIGETDGFLKLITLKEDGLLIGAQVAGAGAS
CCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCEEEEEEECCCCHH
DVIAELGLAIETGMTAEDIALTIHAHPTLGEITMEAAEVAMGHPIHTM
HHHHHHCHHEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKAMLS
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHH
ASHKYETAKHSEDYGIKAENVTLDFSKVQEFKNGVVERLTGGVGSLLKGRKVEIVQGEAY
CCCCCCCCCCCCCCCEEECEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCEE
FVDQNNLKVMTEKASQTYTFKNAIIATGSRPIEIPNFKFNKRVIDSTGALALPEVPKHLV
EEECCCEEEEEECCCCEEEECCEEEECCCCCEECCCCCCCCEEECCCCCEECCCCCCEEE
VVGGGYIGSELGTAYANFGSKVTILEGAKDILGGFEKQMTQVVKKGLKAKGVEIVTEAMA
EEECCEECHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KSAEESENGVKVTYEAKGETHTIEADYCLVTVGRRPNTDELGLEGLGIKMTDRGIIEVDA
HHHCCCCCCEEEEEECCCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEECCCEEEECC
QSRTSVENIYAIGDIVPGPPLAHKASYEGKVAAEAISGEKSEVDYLGIPAVCFTEPELAT
CCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEHHHCCCCCCCEEECCCEEEECCCCEEE
VGYTEAMAKEEGLDFKASKFPYAANGRALSIGETDGFLKLITLKEDGLLIGAQVAGAGAS
CCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCEEEEEEECCCCHH
DVIAELGLAIETGMTAEDIALTIHAHPTLGEITMEAAEVAMGHPIHTM
HHHHHHCHHEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA