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The map label for this gene is purF [H]

Identifier: 222150943

GI number: 222150943

Start: 745296

End: 746735

Strand: Direct

Name: purF [H]

Synonym: MCCL_0693

Alternate gene names: 222150943

Gene position: 745296-746735 (Clockwise)

Preceding gene: 222150942

Following gene: 222150944

Centisome position: 35.45

GC content: 46.18

Gene sequence:

>1440_bases
TTGGAAAGGGGCAATTCACGCATGTATGACATCAGAGGTCTAAACGAAGAGTGTGGCGTGTTCGGTATCTGGGGTCACCC
AAATGCCGCGGAACTCACTTATATGGCGCTGCACTCATTGCAGCACCGTGGTCAGGAAGGTGCCGGTATCGTATGTTCTG
ATGAGAAGAATATCTATGGTGCGCGCGGCATGGGATTGTTACCTGAAGCAATCAGTGAGATGAAACTCGAATCATTAAGC
AGCTTTCATCATGCCATCGGTCATGTACGTTATGCAACGACGGGTGCATCTGAACTGTCCAATGTCCAGCCGTTTATCTT
CAAGCATTCTAAAGGTGATATCGGTCTTGCACATAATGGGAACTTGACGAACGCTGAACAGATTAAGCTTGCACTGGAAT
CTGAAGGTGCAATCTTCCAGACGTCCAGTGATTCTGAAGTGTTAGGACATCTGCTCTTAAAGGCGAAATCAGATAATAAG
CTGAATAATTTGAAAGCATCATTGAACCAGATTAAAGGTGCATTCTCATTTCTCGTATTACATCCTGAAAAGATGTTCGT
AGCCCGTGATTCTCACGGTGTACGTCCACTGATGCTCGGTAAGGTTGACGGAGCATACTGTGTGGCAAGTGAGACGTGTG
CATTTACAGCGGTCGGTGCAGAGTATATTCGTGACATCGAACCAGGTGAAGTGCTGACCCTATCTCATGATGGTCTGGCA
GAAGACGTCTATACAACGAATACACAGTCCAGCATGTGTTCTATGGAGTATGTCTACTTCGCACGTCCGGATTCAGAATT
CCGTAAGCATTCATTATATGAGATACGTAAAGCTTTAGGGCGTAAACTCTATGAGGAGATGAATATCGAAGCGGACGTTG
TAATCGGTGTACCGGACTCTTCATTACAGGCAGCGAAAGGCTTTTCTCTTGCAAGTGGCATTCCGAACGAACAAGGTCTG
CTCAAGAATCGCTACGTTGGCAGAACCTTTATTTCGCCGGACCAGAAGATGCGCGAACGTGCCGTACGCATGAAGCATGC
ACCGATTCGCGATGTCATTGAAGGCAAGCGCGTTGTCGTGATCGATGACTCTATCGTACGTGGTACGACGAGCAAGTTTA
TTGTGAAGACATTGAAGCAGGCAGGTGCGAAAGAAGTGCATATGGCCATCTCAAGTCCACCGCTCATTCATCCGTGTTAT
TACGGTATCGACGTATCAACGCACGCAGAACTTATCGCAGCGAACAAGAGTGTTGCAGAAATCGAAGCAGAGCTGGAAGT
AGATTCACTTACGTATCTATCGGTGGATGGTATGCATGAAGTGTTCCGTTCATTTGATTCTAAAGGCGAATGTAATGCCT
GCTTTACTGGGAACTATCCGATTGAAATCGTGGATCATGTATTACCAGAAGAAAAAGACAAGAAGAAGAAGGGAGTATAA

Upstream 100 bases:

>100_bases
TCGACGGTGCAGAAGCGATCGGTACATTCGGTACAGAATCATTTAAACTATCTACAAACCAAGGGGAAGTTACTTTAGAC
GTGAATCGTATGACGGAGGC

Downstream 100 bases:

>100_bases
TTATGGCTGAAAGTTATAAACAATCAGGCGTTGATATCCATGCTGGGTATGAAGCGGTTGAGCGCATGAAAAAGCATGTG
AACAAGACGATGCGTAAAGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MERGNSRMYDIRGLNEECGVFGIWGHPNAAELTYMALHSLQHRGQEGAGIVCSDEKNIYGARGMGLLPEAISEMKLESLS
SFHHAIGHVRYATTGASELSNVQPFIFKHSKGDIGLAHNGNLTNAEQIKLALESEGAIFQTSSDSEVLGHLLLKAKSDNK
LNNLKASLNQIKGAFSFLVLHPEKMFVARDSHGVRPLMLGKVDGAYCVASETCAFTAVGAEYIRDIEPGEVLTLSHDGLA
EDVYTTNTQSSMCSMEYVYFARPDSEFRKHSLYEIRKALGRKLYEEMNIEADVVIGVPDSSLQAAKGFSLASGIPNEQGL
LKNRYVGRTFISPDQKMRERAVRMKHAPIRDVIEGKRVVVIDDSIVRGTTSKFIVKTLKQAGAKEVHMAISSPPLIHPCY
YGIDVSTHAELIAANKSVAEIEAELEVDSLTYLSVDGMHEVFRSFDSKGECNACFTGNYPIEIVDHVLPEEKDKKKKGV

Sequences:

>Translated_479_residues
MERGNSRMYDIRGLNEECGVFGIWGHPNAAELTYMALHSLQHRGQEGAGIVCSDEKNIYGARGMGLLPEAISEMKLESLS
SFHHAIGHVRYATTGASELSNVQPFIFKHSKGDIGLAHNGNLTNAEQIKLALESEGAIFQTSSDSEVLGHLLLKAKSDNK
LNNLKASLNQIKGAFSFLVLHPEKMFVARDSHGVRPLMLGKVDGAYCVASETCAFTAVGAEYIRDIEPGEVLTLSHDGLA
EDVYTTNTQSSMCSMEYVYFARPDSEFRKHSLYEIRKALGRKLYEEMNIEADVVIGVPDSSLQAAKGFSLASGIPNEQGL
LKNRYVGRTFISPDQKMRERAVRMKHAPIRDVIEGKRVVVIDDSIVRGTTSKFIVKTLKQAGAKEVHMAISSPPLIHPCY
YGIDVSTHAELIAANKSVAEIEAELEVDSLTYLSVDGMHEVFRSFDSKGECNACFTGNYPIEIVDHVLPEEKDKKKKGV
>Mature_479_residues
MERGNSRMYDIRGLNEECGVFGIWGHPNAAELTYMALHSLQHRGQEGAGIVCSDEKNIYGARGMGLLPEAISEMKLESLS
SFHHAIGHVRYATTGASELSNVQPFIFKHSKGDIGLAHNGNLTNAEQIKLALESEGAIFQTSSDSEVLGHLLLKAKSDNK
LNNLKASLNQIKGAFSFLVLHPEKMFVARDSHGVRPLMLGKVDGAYCVASETCAFTAVGAEYIRDIEPGEVLTLSHDGLA
EDVYTTNTQSSMCSMEYVYFARPDSEFRKHSLYEIRKALGRKLYEEMNIEADVVIGVPDSSLQAAKGFSLASGIPNEQGL
LKNRYVGRTFISPDQKMRERAVRMKHAPIRDVIEGKRVVVIDDSIVRGTTSKFIVKTLKQAGAKEVHMAISSPPLIHPCY
YGIDVSTHAELIAANKSVAEIEAELEVDSLTYLSVDGMHEVFRSFDSKGECNACFTGNYPIEIVDHVLPEEKDKKKKGV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=36.5422396856582, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=37.6842105263158, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=39.0396659707724, Blast_Score=320, Evalue=7e-88,
Organism=Saccharomyces cerevisiae, GI6323958, Length=489, Percent_Identity=34.3558282208589, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24659598, Length=501, Percent_Identity=38.9221556886228, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=38.12375249501, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24659604, Length=470, Percent_Identity=39.5744680851064, Blast_Score=322, Evalue=4e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52714; Mature: 52714

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERGNSRMYDIRGLNEECGVFGIWGHPNAAELTYMALHSLQHRGQEGAGIVCSDEKNIYG
CCCCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
ARGMGLLPEAISEMKLESLSSFHHAIGHVRYATTGASELSNVQPFIFKHSKGDIGLAHNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCCCEEEEECCCCEEEECCC
NLTNAEQIKLALESEGAIFQTSSDSEVLGHLLLKAKSDNKLNNLKASLNQIKGAFSFLVL
CCCCHHHEEEEEECCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEE
HPEKMFVARDSHGVRPLMLGKVDGAYCVASETCAFTAVGAEYIRDIEPGEVLTLSHDGLA
CCCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEHHCHHHHHCCCCCCEEEEECCCCC
EDVYTTNTQSSMCSMEYVYFARPDSEFRKHSLYEIRKALGRKLYEEMNIEADVVIGVPDS
CCCEECCCCHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
SLQAAKGFSLASGIPNEQGLLKNRYVGRTFISPDQKMRERAVRMKHAPIRDVIEGKRVVV
CHHHHCCCHHHCCCCCCCCCHHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHCCCEEEE
IDDSIVRGTTSKFIVKTLKQAGAKEVHMAISSPPLIHPCYYGIDVSTHAELIAANKSVAE
ECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCHHHEECCCCHHH
IEAELEVDSLTYLSVDGMHEVFRSFDSKGECNACFTGNYPIEIVDHVLPEEKDKKKKGV
EEEEEEECEEEEEECCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MERGNSRMYDIRGLNEECGVFGIWGHPNAAELTYMALHSLQHRGQEGAGIVCSDEKNIYG
CCCCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
ARGMGLLPEAISEMKLESLSSFHHAIGHVRYATTGASELSNVQPFIFKHSKGDIGLAHNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCCCEEEEECCCCEEEECCC
NLTNAEQIKLALESEGAIFQTSSDSEVLGHLLLKAKSDNKLNNLKASLNQIKGAFSFLVL
CCCCHHHEEEEEECCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEE
HPEKMFVARDSHGVRPLMLGKVDGAYCVASETCAFTAVGAEYIRDIEPGEVLTLSHDGLA
CCCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEHHCHHHHHCCCCCCEEEEECCCCC
EDVYTTNTQSSMCSMEYVYFARPDSEFRKHSLYEIRKALGRKLYEEMNIEADVVIGVPDS
CCCEECCCCHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
SLQAAKGFSLASGIPNEQGLLKNRYVGRTFISPDQKMRERAVRMKHAPIRDVIEGKRVVV
CHHHHCCCHHHCCCCCCCCCHHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHCCCEEEE
IDDSIVRGTTSKFIVKTLKQAGAKEVHMAISSPPLIHPCYYGIDVSTHAELIAANKSVAE
ECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCHHHEECCCCHHH
IEAELEVDSLTYLSVDGMHEVFRSFDSKGECNACFTGNYPIEIVDHVLPEEKDKKKKGV
EEEEEEECEEEEEECCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]