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The map label for this gene is capI [H]

Identifier: 222150924

GI number: 222150924

Start: 727324

End: 728334

Strand: Direct

Name: capI [H]

Synonym: MCCL_0674

Alternate gene names: 222150924

Gene position: 727324-728334 (Clockwise)

Preceding gene: 222150923

Following gene: 222150925

Centisome position: 34.6

GC content: 30.46

Gene sequence:

>1011_bases
ATGAAAATACTAGTTACAGGAATAGCTGGTTTTATTGGATCAAATTTAGCAAAAAAATTAAAAGAAAAAGGGCATGAAGT
ATTTGGAATCGATAATTTAAATAATTACTATTCTGTAGAACTGAAAAAAGACCGTTTATCGAAATTTTTAAATAATGAAT
TTAAAAACTATGAAATTAACTTAGAAAATTATGAAGCTGTGAAAAAAGTATTTGAACAAGAGAAACCTGAAGTAGTAATT
AATTTAGCTGCTCAAGCAGGAGTAAGATATAGTCTAGAGAATCCATTTACTTATATTCAAAGTAACGTAAATGGTTTTAT
GAGTATTTTAGAGGCATGTCGTCACAATAATGTTAAAAATTTGATTTATGCTTCGTCTAGTTCAGTGTATGGAGCAAACA
CATCTCTCCCATTTTCTACTTCAGACAATATCGATCATCCAATCAGTCTATATGCTGCCACTAAAAAATCAAATGAATTG
ATGGCACATACATATAGCCATTTATTTAATCTTCCTACCACAGGTTTAAGATTCTTTACTGTTTATGGACCATGGGGAAG
ACCTGATATGGCATTATTTAAATTCACAAAAAATATTCTAAACAACGAATCAATAGATGTATACAATAACGGTAATATGA
TGAGAGATTTCACTTATGTTGATGATATTGTTGAAGCTATCTCTCGCTTAGTAGAACGTCCAGCGCAACCAAATAAAGAG
TGGAGTGGAGATAACCCATCACCAGATTCTAGTTATGCCCCATATAAGGTATATAATATTGGGAACAATGCACCAGTTAA
ATTGATGGAATTTATTGAAGCAATTGAAACTAGAACAGGCATTGAAGCTAAAAAGAATTTTATGGAATTGCAAGCAGGTG
ATGTTCCTCAAACATATGCCAATGTCGATGATTTATTTAGAGATATAGATTTCAAACCACAAACGAACATTCAAGATGGA
GTTAATAATTTTGTTGATTGGTATATGAATTATTACGATATTAAAAAATAA

Upstream 100 bases:

>100_bases
CAAGAAGCGTCGTTGCTGCAAATGCTATTGTTAATAGAGATGTTATGAGTAATAATTTGGTTGGTGGAATACCTATAAAA
ATCATAAAGGAGATTTAAAA

Downstream 100 bases:

>100_bases
TTTTAAAGGATGTATATAATGAATAGAAAAATCGCAGTAGTAGGATTAGGTTATGTAGGTTTACCCGTAGCAGCTGCATT
TGGAAAATTACATGAAGTTA

Product: capsular polysaccharide biosynthesis protein CapI

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKILVTGIAGFIGSNLAKKLKEKGHEVFGIDNLNNYYSVELKKDRLSKFLNNEFKNYEINLENYEAVKKVFEQEKPEVVI
NLAAQAGVRYSLENPFTYIQSNVNGFMSILEACRHNNVKNLIYASSSSVYGANTSLPFSTSDNIDHPISLYAATKKSNEL
MAHTYSHLFNLPTTGLRFFTVYGPWGRPDMALFKFTKNILNNESIDVYNNGNMMRDFTYVDDIVEAISRLVERPAQPNKE
WSGDNPSPDSSYAPYKVYNIGNNAPVKLMEFIEAIETRTGIEAKKNFMELQAGDVPQTYANVDDLFRDIDFKPQTNIQDG
VNNFVDWYMNYYDIKK

Sequences:

>Translated_336_residues
MKILVTGIAGFIGSNLAKKLKEKGHEVFGIDNLNNYYSVELKKDRLSKFLNNEFKNYEINLENYEAVKKVFEQEKPEVVI
NLAAQAGVRYSLENPFTYIQSNVNGFMSILEACRHNNVKNLIYASSSSVYGANTSLPFSTSDNIDHPISLYAATKKSNEL
MAHTYSHLFNLPTTGLRFFTVYGPWGRPDMALFKFTKNILNNESIDVYNNGNMMRDFTYVDDIVEAISRLVERPAQPNKE
WSGDNPSPDSSYAPYKVYNIGNNAPVKLMEFIEAIETRTGIEAKKNFMELQAGDVPQTYANVDDLFRDIDFKPQTNIQDG
VNNFVDWYMNYYDIKK
>Mature_336_residues
MKILVTGIAGFIGSNLAKKLKEKGHEVFGIDNLNNYYSVELKKDRLSKFLNNEFKNYEINLENYEAVKKVFEQEKPEVVI
NLAAQAGVRYSLENPFTYIQSNVNGFMSILEACRHNNVKNLIYASSSSVYGANTSLPFSTSDNIDHPISLYAATKKSNEL
MAHTYSHLFNLPTTGLRFFTVYGPWGRPDMALFKFTKNILNNESIDVYNNGNMMRDFTYVDDIVEAISRLVERPAQPNKE
WSGDNPSPDSSYAPYKVYNIGNNAPVKLMEFIEAIETRTGIEAKKNFMELQAGDVPQTYANVDDLFRDIDFKPQTNIQDG
VNNFVDWYMNYYDIKK

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=25.8160237388724, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI56237023, Length=326, Percent_Identity=24.8466257668712, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI56118217, Length=326, Percent_Identity=24.8466257668712, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI189083684, Length=326, Percent_Identity=24.8466257668712, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI42516563, Length=343, Percent_Identity=25.9475218658892, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=24.0223463687151, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI48994969, Length=361, Percent_Identity=24.9307479224377, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=23.4285714285714, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982038, Length=358, Percent_Identity=23.1843575418994, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=356, Percent_Identity=23.314606741573, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=242, Percent_Identity=28.9256198347107, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=345, Percent_Identity=25.7971014492754, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=21.7008797653959, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=350, Percent_Identity=24, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19923002, Length=347, Percent_Identity=24.207492795389, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=26.0355029585799, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38385; Mature: 38385

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGIAGFIGSNLAKKLKEKGHEVFGIDNLNNYYSVELKKDRLSKFLNNEFKNYEIN
CEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECHHHHHHHHCCCCCCEEEC
LENYEAVKKVFEQEKPEVVINLAAQAGVRYSLENPFTYIQSNVNGFMSILEACRHNNVKN
CHHHHHHHHHHHCCCCCEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
LIYASSSSVYGANTSLPFSTSDNIDHPISLYAATKKSNELMAHTYSHLFNLPTTGLRFFT
EEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEE
VYGPWGRPDMALFKFTKNILNNESIDVYNNGNMMRDFTYVDDIVEAISRLVERPAQPNKE
EECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCC
WSGDNPSPDSSYAPYKVYNIGNNAPVKLMEFIEAIETRTGIEAKKNFMELQAGDVPQTYA
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHH
NVDDLFRDIDFKPQTNIQDGVNNFVDWYMNYYDIKK
CHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVTGIAGFIGSNLAKKLKEKGHEVFGIDNLNNYYSVELKKDRLSKFLNNEFKNYEIN
CEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECHHHHHHHHCCCCCCEEEC
LENYEAVKKVFEQEKPEVVINLAAQAGVRYSLENPFTYIQSNVNGFMSILEACRHNNVKN
CHHHHHHHHHHHCCCCCEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
LIYASSSSVYGANTSLPFSTSDNIDHPISLYAATKKSNELMAHTYSHLFNLPTTGLRFFT
EEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEE
VYGPWGRPDMALFKFTKNILNNESIDVYNNGNMMRDFTYVDDIVEAISRLVERPAQPNKE
EECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCC
WSGDNPSPDSSYAPYKVYNIGNNAPVKLMEFIEAIETRTGIEAKKNFMELQAGDVPQTYA
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHH
NVDDLFRDIDFKPQTNIQDGVNNFVDWYMNYYDIKK
CHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]